Objective To investigate the effects of substance P (SP) pretreatment on the expression of β1-adrenoreceptor(β1-R) induced by norepinephrine (NE) in cultured neonatal rat cardiomyecytes.Methods The cardiomyocytes obtained from 1-3 day old SD rats were cultured for 72 h.The experiment was performed in 2 parts.In part 1 the cells were seeded in 15 well plate and randomly divided into 5 groups (n=3 wells each):control group (C1)and 4 NE groups were exposed to NE 10-9, 10-8,10-7,10-6 mol/L respectively (NE1,2,3,4).In part Ⅱ the.cells were seeded in 12 well plate and randomly divided into 4 groups (n=3 wells each): group I control (C2): group II NE 10-7 tool/L; group Ⅲ (SN)was pretreated with SP 10-6 mol/L 30 minbefore NE 10-7 mol/L and group IV (NSN)was treated with NK-1 receptor antagonist (NK-1 tachykinin receptor antagonist,S3144) 30 min before SP pretreatment.After exposure to NE for 3 h the expression of β1-R in the rat cardiomyecytes was detected using flow cytometry.Results In part Ⅰ the expression of β1-R was significantly higher in group NE1-3 than in control group (C1),with the highest expression in group NF3.In part Ⅱ the expression of β1-R was significantly higher in group Ⅱ (NE) than in control group (C2) while lower in group Ⅲ (SN) than in control group Ⅱ.(NE).There was no significant difference in the expression of β1-R between group Ⅰ (C2) and group Ⅲ and Ⅳ (NSN).Conclusion Substance P pretreatment can inhibit the up-regulation of β1-R expression in cultured rat cardiomyocytes induced by norepinephrine through activating NK-1 receptor.

Objective To analyze the clinical characteristics, diagnosis, therapy and prognosis of acute pulmonary embolism (APE) following surgery so as to advise clinicians to pay more attention to and prevent postoperative APE. Method Thirty-one APE patients following surgery treated in the recent 8 years were analyzed retrospectively. The clinical features, diagnosis, therapy and prognosis of patients were analyzed. Results (1) Post-operative APE patients accounted for 21.9% of all APR patients during that period. The mortality of patients after surgical operation was 3.2%. (2) APE following surgery often occurred in patients after operations of spine, abdomen, gynecological surgery and point replacement as well as in patients with malignant tumor. The APE following surgery often occurred in the first week after surgery. The APE after surgery for malignant tumor occurred sooner. (3) Among many clinical manifestations, dyspnea (90.3%) was the commonest one. Other manifestations included chest pain, palpitation and syncope. The typical triad of dyspnea, hemoptysis and cheat pain was rarely seen. (4) The venous thrombolysis was a absolute contra-indication for massive PE after surgery. Catheter embolectomy and fragmentation, and surgical embolectomy were the alternative treatment. Conclusions Surgery is the essential risk factor of APE. When patients present dyspnea, chest pain or syncope, clinicians should pay attention to APE. Anticoagulation and embolectomy can improve the prognosais of the patients.

Objective To explore the correlation between the family of p16 gene inactivation and prognosis of leukemia, and then to clarify, the pathogenesis of leukemia, and monitor process of leukemia. Methods We used polymerase chain reaction(PCR) to study p16, p15, p18, p19 gene homozygous deletion,by using methylation-sensitive enzyme and PCR technology to investigate p16, p15, p18, p19 gene methylation in leukemia. Results The effective rate with p16 and p15 gene activiation was 27 cases (84.38 %), the effective rate with p16 and p15 gene inactiviation was 11 cases (28.95%), and the total effective rate with p16 and p15 gene activiation was higher than p16and p15 gene inactivation. In case to use single and multi factor Logistic regression, effective rate in cases with p16 and p15 gene inactiviation was lower than that with p16 and p15 gene activation. Conclusion It might be one of parameters for forcasting progression, relapse and prognosis in AL.

The study of tumors' proteomics is a research hotspot in China and abroad in recent years.The development of theory and technology in proteomics has provided new ideas and research field for cancer research.This paper reviewed the commonly used technical means of proteomics and its research progress on diagnosis,therapy and other aspects of pancreatic carcinoma.

Objective To evaluate the effects of different time courses and depths of acute normovolemic hemodilution (ANH) with hematocrit (Hct) on injury to the mucous membrane of small intestines in rabbits.Methods Thirty-two adult rabbits,weighing 2.0-2.5 kg,were randomly divided into 4 groups (n=8 each) using a random number table:control group (group C),Hct 18％ group (H1 group),Hct 15％ group (H2 group) and Hct 12％ group (H3 group).The animals were anesthetized with iv 20％ urethane 4 ml/kg,tracheostomized and mechanically ventilated.Blood samples were taken from the left femoral artery.Blood withdrawn from the femoral artery =2 ×body weight× body fluid per kilogram × (initial Hct-target Hct) ÷ (initial Hct+target Hct),and was simultaneously replaced with the equal volume of 6％ hydroxyethyl starch 130/0.4 infused over 30 min via the left femoral vein until the target Hct was achieved in H1,H2 and H3 groups.At 30 min of stabilization after puncture (T0) and 2,4 and 8 h after ANH (T1-3),blood samples were obtained from the superior mesenteric artery and vein for blood gas analysis and for determination of plasma concentrations of tumor necrosis factor-α (TNF-α) in the superior mesenteric vein.Oxygen extraction rate (ERO2) was calculated.Small intestinal mucosal tissues were obtained at T3 to observe the pathological changes with light microscope.Results Compared with those at T0,the plasma concentrations of TNF-α at T3 and ERO2 at T1-3 were significantly increased in H1 group,and the plasma concentrations of TNF-α at T3 and ERO2 at T1-3 were increased in H2 and H3 groups.The injury to the mucous membrane of small intestine was mild in group H1,and was severe in H2 and H3 groups.Conclusion ANH performed with 6％ hydroxyethyl starch causes injury to the mucous membrane of small intestine when Hct is 18％ and the time course ≥ 8 h,and when Hct ≤ 15％ and the time course ≥ 2 h in the rabbits.

Three-dimensional (3D) laparoscopic surgery has been developed rapidly in China since 2012.Based on mature two-dimensional (2D) laparoscopic surgery,3D laparoscopic surgery has the same technique as 2D,with similar indication as 2D as well.However,3D laparoscopic surgery can offer better depth description,more accurate spatial location,and less error in operation.It might offer better visional experience if hybrid with virtual reality or with 4K in the future.But in current status,laparoscopy with 3D technique is only a technique with innovation,but not a revolutionary technique.

With the standardized training and spreading,laparoscopic total mesorectal excision for rectal cancer has been spread nationwide.The oncological adequacy and long-term outcomes have also been confirmed by series of randomized controlled trials such as COLOR Ⅱ in Europe.There were still some technical issues,such as safe surgical plane,surgical approach,lymph node dissection,and function-preserving,which were still under debate.In the current article,authors will discuss the issues with clinical experiences about preserving of Denonvilliers facial,preserving of pelvic autonomic nerve,strategy of surgical approach,dissection of lateral lymph nodes,and necessity of protective diverting stoma.

Objective To study the relationship of total serum testosterone and free testosterone with the age of men. Methods A total of 136 heathy men were classified into six groups. Enzyme immunoassay was applied to measure the serum level of testosterone and free testosterone, and then the relationship between the androgen change and the age was analyzed. Results The level of testosterone of 20-, 30-, 40-, 50-, 60- and 70-78 age group were (6.208?2.275),(4.960?1.719),(5.910?3.054),(4.964?1.531) ,(3.967?2.514) and (4.146?2.497) mg/L respectively.Free testosterone concertrations were:(23.300?9.530),(22.060?12.627),(16.290?6.858),(14.782?6.851) ,(14.005?8.724) and (12.256?7.462) ng/L respectively. The level of blood testosterone decreased in 60-year age groups and the difference between 20-, 30-year old group and 60-year old group was significant (P

Objective The purpose of this study was to determine if parafascicular nucleus of thalamus is involved in the nociceptive stimulation evoked by coronary artery occlusion-induced acute myocardial ischemia and to investigate the effect of fentanyl on this nociceptive stimulation. Methods Male SD rats weighing 260-300 g were operated upon under general anesthesia with intraperitoneal urethane (1.2 g ? kg -1 ) and local infiltration of the skin incision. The discharges of pain-sensitive neurons (PSN) were recorded for 20 seconds every 5 min using single-barrel glass electrode before and after the coronary artery occlusion ( CAO) . The study was divided into 3 groups: group Ⅰ CAO alone ( n = 9); group Ⅱ CAO + fentanyl ( n = 6) : fentanyl 0.01 mg ?kg-1 was administered iv 15 min after CAO; group Ⅲ CAO + fentanyl + naloxone ( n = 6) : naloxone 0.04 mg? kg-1 was administered iv 15 min after intravenous fentanyl administration. Results The discharge frequency was significantly increased following CAO and peaked within 5-10 min after CAO and maintained for 60 min. The increased frequency of nociceptive discharge was significantly inhibited within 10 min after fentanyl and intravenous naloxone could completely antagonize the inhibitory effect of fentanyl. Conclusion Visceral pain can be evoked by acute myocardial ischemia induced by coronary artery occlusion. Thalamic parafascicular nucleus is involved in the perception of nociception in CNS. Opioid receptors play a critical role in the modulation of the nociception.

In recent years,more and more Macrolide resistance of Mycoplasma pneumoniae becomes a hot research.The current studies suggest that the mechanisms of Macrolide resistance are drug-binding site mutations,ribosome methylation,drug efflux,and drug related fire-fighting,in which the drug target 23srRNA gene 2063,2064 mutation is significant.It has been reported that drug-resistant patients have the poor effect,including the long total febrile days and high dressing.For treatment of drug-resistant patients,most studies still use the macrolide,early use of corticosteroids may be effective.It is uncertain whether the increase in resistant strains is related with the use of macrolide.It is significant to carry out future researches in this area for clinical therapy and put off the prevalence of drug-resistant strains.