BACKGROUND:The extracelular matrix with removal of cels and soluble proteins can maintain the normal shape of organs and matrix components. OBJECTIVE:To prepare the acelular matrix of lung tissue using perfusion method. METHODS:Forty Wistar rats were randomly divided into two groups: under routine anesthesia, the chest was open to obtain complete lung tissue for construction of rat lung acelular matrix scaffold using Langendorff perfusion model in experimental group, and there was no treatment in control group. Lung tissue color and shape were observed and recorded dynamicaly. A smal tissue from different sites of the lung was taken from each group and observed histologicaly under electron microscope. The elastic fibers and connective tissues were highlighted by a Weigert or von Gieson staining, respectively. Hematoxylin-eosin staining was also employed. RESULTS AND CONCLUSION:After perfusion with 1% sodium deoxycholate, the lung tissue of the experimental group gradualy appeared with a piecewise and lobulated translucent appearance in white color from the inside to the outside, and the lung structure was clear. Eventualy, the lung tissue became uniform white translucent. The Weigert-Von Gieson staining and hematoxylin-eosin staining showed that there were a large amount of cels in fresh lung tissue sections of the control group, including capilaries, fibroblasts and endothelial cels; the cels were arranged neatly, and had complete alveolar structure; the elastic fiber structure was clear, and the colagen fiber was arranged neatly and compactly. In the experimental group, the lung cels nearly disappeared, and the alveolar structure was stil intact but in a loose state; the elastic fibers were preserved wel and the colagen fibers were loosely arranged. These findings indicate that the perfusion method can be used to effectively construct the lung matrix scaffold in rats.

The flavin-containing monooxygenase 3 (FMO3) is an important hepatic microsomal enzyme. Numerous mutations of FMO3 gene have been reported, and polymorphic varients of the gene have been identified. Several studies indicated that variability in the expression of FMO3 involved in some nitrogen, or sulfur-containing durg metabolism. This review summarizes the genetic polymorphism of FMO3 and its role in drug metabolism and toxicity.
Drug-Related Side Effects and Adverse Reactions ; Humans ; Oxygenases ; genetics ; Polymorphism, Genetic

OBJECTIVE To investigate the application of risk management in the practice of the sterilization supply department.METHODS Through the risk management theory to identify and evaluate the latent risk factor and achieve the purpose of preventing and controlling.To improve the awareness and ability of risk guarding among nurses by strengthening professional training and establish and perfect the regulations,we insisted on "zero fault" management idea and adopted irregular spot-checking and quality overruled system.We also bought the results into bonus-penalty system and continue for the quality improvement.RESULTS It strengthened the risk and quality cognition and the responsibility of the nurses and reduced the occurrences of the small defects and defaults.CONCLUSIONS The risk and quality management is binding with each other.Through the applications of risk management the most high quality sterilized goods are offered to our clinical practice due to the decreasing in risk factors.

Objective:To analyze and compare pharmacology cognizing between five-year and three-year systems students in medicine to provide materials for teaching innovation.Method:To gather feedbacks from the students and to analyze and induce the cases by EXCEL software.Results:The effectiveness of medicament is the focus.But five-year and three-year systems students concern different problems.57.1% questions raised by three-year system students are about the effectiveness and the effective factors of medicament,while 53.2% by five-year system students are about the effectiveness and the mechanism of medicament.Conclusion: There have been certain differences of pharmacology cognizing between five-year and three-year systems students.

Objective To evaluate the efficacy of superselective renal artery embolization in the treatment of iatrogenic renal hemorrhage. Methods The clinical data of 31 iatrogenic renal hemorrhage patients who had underwent superselective renal artery embolization were retrospectively analyzed. Results All patients were found to have bleeding site by contrast examination, including pseudoaneurysm in 21 cases, renal arteriovenous fistula in 3 cases, extravasation of contrast media in 4 cases, and pseudoaneurysm and renal arteriovenous fistula in 3 cases. Microcoil embolization was used in 20 patients, and microcoil embolization combined with gelatin sponge was used in 12 patients. Thirty patients got successful embolization once, and success rate was 96.8%(30/31). One patient got successful embolization in the second time. There were no serious complications and no recurrence of renal hemorrhage. Conclusions Superselective renal artery embolization in the treatment of iatrogenic renal hemorrhage has the advantages of exact hemostasis, less trauma and fewer complication, and can retain the normal renal tissue maximumly.

Acoustic Stimulation ; Animals ; Auditory Perception ; physiology ; Behavior, Animal ; physiology ; Noise ; adverse effects ; Rabbits ; Sound ; Swine

To explore the mechamisms of bactericidal neutralizing endotoxin peptide(BNEP), a synthetic peptide mimicking bactericidality/permeability-increasing protein (BPI). The affinities of BNEP for LPS and Lipid A were determined with biosensor technology, and the ability of BNEP neutralizing LPS in vitro was tested by quantitative limulus amoebocyte lysate assay. The results showed that BNEP had high affinities for both LPS and Lipid A. The Kd value for LPS was at the level between 25.8 and 48.8nmol/L and for Lipid A from 11.8 to 21.8nmol/L. When 8?g/ml of BNEP was used, it could completely neutralize the concentration of 2ng/ml of LPS in vitro. It is concluded that BNEP has high binding affinities for both LPS and Lipid A. Our results also suggest that the binding site of LPS is at the glucosaminyl-?1'-6-glucosamine disaccharide of Lipid A. The binding activity of BNEP for LPS is in accord with its neutralizing activity for LPS.

Objective To study the relationship between genetic polymorphisms of cytochrome P450 2E1(CYP2E1) and susceptibility to gastric cancer. Methods Genotype of CYP2E1 was determined by polymorphism (PCR-RFLP) analysis on DNA in 92 patients with gastric cancer and 92 controls in case-control study. Results The results showed that the frequency of the wild-type genotype (C1/C1) detected by RsaⅠ digestion was 66.3% and 48.9% in gastric cancer group and controls, respectively (P

Objective To evaluate the application effect of clinical nursing pathways in acute myocardial infarction patients by using the Meta-analysis.Methods Published randomized controlled trials (RCT) in acute myocardial infarction patients were searched and screened in CNKI,VIP,Wanfang database under present standards.The quality of the included studies was evaluated by certain standards.The Review Manager 5.0 software was taken for analysis.Results Totally 18 studies including 1877 cases were eligible to the criteria (894 in the experimental group and 983 in the control group) altogether.The Meta-analysis showed there was significant difference between the experimental group and the control group in hospitalization days,patients' satisfaction degree and rate of mastering knowledge.Significant difference existed between the experimental group and the control group in bedridden time,complication rate and the recurrence rate of myocardial infarction.Conclusions Application of clinical nursing pathway in acute myocardial infarction patients can shorten hospitalization days,increase patients' satisfaction degree and rate of mastering knowledge,decrease bedridden time,complication rate and the recurrence rate of myocardial infarction.

Objective:To explore the discrimination against the patients with mental disorders and current situation of awareness of mental health knowledge of medical postgraduates and their relationship.Methods:A questionnaire survey was conducted among 132 medical postgraduates in October 2016 using the assessment scale of discrimination against patients with mental disorders and mental health knowledge awareness questionnaire,which derived from mental health work indicators survey and evaluation program issued by the General Office of the Ministry of Health in 2010.Results:There was significant gender difference (P ＜0.01)in the overall score and the score of each dimension of assessment scale of discrimination against patients with mental disorders,and the score of mental health knowledge was (13.93-± 3.13).There was significant negative correlation between scores of assessment scale of discrimination against patients with mental disorders and mental health knowledge.Multiple linear regression analysis showed that awareness of mental health knowledge could predict the discrimination against the patients with mental disorders and all its factors.Conclusion:Awareness of mental health knowledge in medical post graduates can predict the discrimination against the patients with mental disorders.Medical universities should carry out corresponding education and publicity work with pertinence.