This investigation was made on 30 adult female rats weighing approximately 150～200g. during estrus as judged from vaginal smear examination. Ovaries which investigated with electron microscope, were fixed in 3％ glutaraldehyde. Other ovaries were embeded in paraffin and examined under light microscope.Theca folliculi of large antral follicles were examined with electron microscope. Three kinds of theca cells were distinguished. They were secretory cell, fibroblast and smooth muscle cell. The secretory cell showed the characterastics of the steroidproducing cell, having smooth endoplasmic reticulum, mitochondria and lipid droplets. The fibroblast contained rough endoplasmic reticulum, free ribosomes and mitochondria in the cytoplasm. The smooth muscle cell was located in the outer layer of theca folliculi, and its structural features were typical according to the criteria of Somlyo and Somlyo: (1) pinocytotic vesicles of the cytoplasmic membranes; (2) myofilaments in the cytoplasm; (3) dense bodies intimately related to the myofilaments; and (4) location of mitochondria and endoplasmic reticulum at the nuclear poles.The ultrastructure of smooth muscle cell, its differentiation and other problems were discussed.

The report of ultrastructure of dog ovarian follicle wall has not been seen yet. This investigation was made on 5 adult dogs in the estrus stage of the cycle and examined with electron microscope. There are granular cells, fibroblasts and secretory cells in the ovarian follicle wall, The typical smooth muscle cell is absent.

Objective To observe the manifestations of MSCT and CT virtual endoscopy (CTVE) images of pleural windows in spontaneous pneumothorax.Methods MSCT data of 73 patients of spontaneous pneumothorax were analyzed.Taking pneumatized sac as the center,thin cross sectional planer (1 mm thickness) MPR and CTVE were reconstructed.Then the size and location of pleural windows,form of pleural surface defect or niche and the relationship with the chest were observed.Results In all 73 patients of spontaneous pneumothorax,27 pleural windows were detected in 15 patients with MSCT thin cross-sectional planer reconstruction image.Pleural windows were observed on the left side in 11 patients,while on the right side in 4 patients.Most of pleural windows located at apex (15/27,55.56％),followed by anterior chest wall (7/27,25.93％) and mediastinum (5/27,18.52％).The connection of pleura window with thorax was shown in 23 (23/27,85.19％).Small defection on parietal pleural with pneumatized sac bulging was seen in all pleural windows,with circular,elliptic,short columnar or hockey shapes.On the front view of CTVE,pleural windows showed niche on parietal pleural surface with round or oval form,and the bottom of the pneumatized sac could be straightly seen in 19 pleural windows.The walls were flat,tunnel shaped in 6 pleural windows,while cavernous shaped in other pleural windows.Conclusion Pleural windows often displayed as small diverticulum like shadows on parietal pleural surfaces,and the pathogenesis might be associated with negative pressure increased in local pleural cavity and pleural damages.

OBJECTIVE: To design and construct a graphene oxide (GO)/silver nitrate (Ag₃PO₄)/chitosan (CS) composite coating for rapidly killing bacteria and preventing postoperative infection in implant surgery. METHODS: GO/Ag₃PO₄ composites were prepared by ion exchange method, and CS and GO/Ag₃PO₄ composites were deposited on medical titanium (Ti) sheets successively. The morphology, physical image, photothermal and photocatalytic ability, antibacterial ability, and adhesion to the matrix of the materials were characterized. RESULTS: The GO/Ag₃PO₄ composites were successfully prepared by ion exchange method and the heterogeneous structure of GO/Ag₃PO₄ was proved by morphology phase test. The heterogeneous structure formed by Ag₃PO₄ and GO reduced the band gap from 1.79 eV to 1.39 eV which could be excited by 808 nm near-infrared light. The photothermal and photocatalytic experiments proved that the GO/Ag₃PO₄/CS coating had excellent photothermal and photodynamic properties. In vitro antibacterial experiments showed that the antibacterial rate of the GO/Ag₃PO₄/CS composite coating against Staphylococcus aureus reached 99.81% after 20 minutes irradiation with 808 nm near-infrared light. At the same time, the composite coating had excellent light stability, which could provide stable and sustained antibacterial effect. CONCLUSION GO/Ag₃PO₄/CS coating can be excited by 808 nm near infrared light to produce reactive oxygen species, which has excellent antibacterial activity under light.
Chitosan ; Silver Nitrate ; Titanium ; Anti-Bacterial Agents/pharmacology* ; Coloring Agents