BACKGROUND:Studies have shown that theβ-tricalcium phosphate can be combined with other macromolecular compounds to improve the mechanical strength and histocompatibility, meeting the requirements of clinical application. ＜br> OBJECTIVE:To evaluate the selection and preparation methods of composite scaffold in bone tissue engineering, and to describe the problems we are facing. ＜br> METHODS:The first author retrieved PubMed, Google Scholar, CNKI, Wanfang, and VIP databases by computer using the keywords of“β-tricalcium phosphate,β-TCP, PLGA, PLA, scaffold, prepare”in English and Chinese, respectively. ＜br> RESULTS AND CONCLUSION:β-tricalcium phosphate combined with polylactic acid-glycolic acid/polylactic acid as bone tissue engineering scaffolds has achieved a great progress. At present, the organic polymer composite scaffolds can be prepared by microsphere sintering, fiber bonding, solvent casting/particulate leaching, emulsification/freeze drying technology, gas foaming method, phase separation technology, rapid prototyping technology and electrostatic spinning method.β-tricalcium phosphate composite scaffolds with polylactic acid-glycolic acid/polylactic acid can be customizable so as to meet different requirements for bone tissue engineering, which can improve the porosity, mechanical properties and biodegradation by improving fabrication techniques and processings, ratio of raw materials and fil er proportion.

AlM:To compare the results of lOL Master, contact and immersion A-scan ultrasound measurements for anterior chamber depth ( ACD ) , and evaluate the difference and consistency.METHODS:Fifty-eight cases (98 eyes) with age-related cataract during July to October in 2013 did the A-scan ultrasound with contact and immersion measurements and lOL Master to get the results of ACD. Difference in measurements between methods was assessed using the variance analysisi. Consistency was assessed using Bland-Altman.RESULTS:The ACD measured by lOL Master was 2. 31~3. 90mm, the mean was 3. 03 ± 0. 38mm. The ACD measured by contact A- scan ultrasound was 1. 51 ~4. 06mm, the mean was 2. 88 ± 0. 56mm. The ACD measured by immersion A-scan ultrasound was 1. 99 ~4. 17mm, the mean was 3. 17±0. 38mm. The results of lOL Master and contact A - scan ultrasound had statistical differences (P=0. 022<0. 05). The results of lOL Master and immersion A - scan ultrasound had statistical differences (P=0. 031<0. 05). The results of contact A-scan ultrasound and immersion A-scan ultrasound had statistical differences (P=0. 000<0. 05). The consistency between three methods was poor. CONCLUSlON: The rank of ACD of patients with cataract is immersion A-scan ultrasound, lOL Master and contact A-scan ultrasound. The consistency is poor, and the three methods can’t be interchanged clinically.

Objective To explore the characteristics of uhrasonography of hepatobiliary cystadenoma. Methods The ultrasonographic characteristics of 5 cases with hepatobiliary cystadenoma were retrospectively analysed, and compared with the operation and pathology. Results The ultrasonography of 5 cases was multilocular cystic mass,which seems to be,but not indeed communicating with the biliary tree,in which irregular septation was demonstrated in two-dimensional structure and also with perforator vessels along in color Doppler flow imagineg. And in some cases small papillary tuft and small loci of calcification was noted on the septation. Conclusions Muhilocular cystic mass,locating along the biliary tree, however not communicating with the the biliary tree, is an important diagnostic basis of biliary cystadenoma. And it should be distinguished with other cystic tumor in the liver.

Objective:To observe therapeutic effect of captopril treatment on aged patients with essential hyperten‐sion (EH) and complications and evaluate its safety .Methods :A total of 116 aged EH patients ,who were compli‐cated with dyslipidemia ,diabetes mellitus etc . were selected from our hospital .According to random number table , they were randomly and equally divided into atenolol control group and captopril group (received captopril based on treatment of atenolol control group) .Blood pressure ,heart rate ,fasting plasma glucose (FPG) and blood lipids were observed and compared between two groups ,the therapeutic effect and adverse reactions were assessed .Re‐sults:After treatment ,total effective rate of captopril group was significantly higher than that of atenolol control group (98.28% vs .87.93% ,P<0.05);compared with atenolol control group ,there were significant reductions in levels of blood pressure [ (131.20 ± 11.02/82.01 ± 6.75) mmHg vs .(115.62 ± 10.27/75.68 ± 5.21) mmHg] ,heart rate [ (65.14 ± 6.32) beats/min vs .(57.21 ± 4.02) beats/min] ,FPG [ (4.75 ± 1.36) mmol vs .(3.65 ± 1.24) mmol] ,total cholesterol [ (2.69 ± 0.58) mmol/L vs .(2.10 ± 0.41) mmol/L] ,triglyceride [ (0.96 ± 0.41) mmol/L vs .(0.72 ± 0.35) mmol/L] and low density lipoprotein cholesterol [ (1.95 ± 0.57) mmol/L vs .(1.71 ± 0.40) mmol/L] in captopril group ,P<0.05 or <0.01 .There were no obvious adverse reactions occurred in both groups . Conclusion:Compared with atenolol ,captopril treatment possesses better therapeutic effect without adverse reac‐tions in aged patients with essential hypertension and complications ,which is worth extending .

Objective] Observing Cortex Lycii Radicis' effect(Cortex Lycii) on rat insulinoma cells(INS-1) proliferation and apoptosis. To explore the mechanism of Cortex Lycii on Pancreatic Beta Cell Proliferation and apoptosis.[ Methods] After primary culture, cells were randomly divided into blank control group:control,11.1mmol·L-1 glucose,HG,30mmol·L-1 glucose, HG+Cortex Lycii(1g·L-1), HG+Cortex Lycii(2g·L-1), HG+Cortex Lycii(4g·L-1), the survival rate of cells was observed by cell counting kit-8(CCK-8);the apoptosis rate was observed by Annexin V stammg. [Results] ①Compared with HG the better effect of cell proliferation groups of Cortex Lycii(P<0.01), the best is group of Cortex Lycii(2g·L-1)(P<0.05) ②Compared with HG group the higher survival rate is group of Cortex Lycii(P<0.01), the lower apoptosis rate of Cortex Lycii(2g·L-1) compared with Cortex Lycii(1g·L-1). [Conclusion] Cortex Lycii can promote the proliferation of pancreatic beta cell, inhibit the apoptosis to protect the pancreatic beta cell. The optimal concentration is 2g·L-1.

Objective Evaluated NOS expression in bladder tissue from the patients with transitional cell carcinoma (TCC) of the bladder and studied its relationship with angiogenesis. Methods Bladder carcinoma tissue specimens were procured from 58 patients with TCC and 14 cases of benign bladder tissue as contrast group. NOS immunohistochemistry was performed on all tissue specimens and microvessal density (MVD) was counted by endothelial cells immunostained. Results Inducible NOS specific proteins were found in 47 of 58 bladder cancer specimens but not in control bladder tissue. The malignant cells and inflammatory cells within the carcinomas were highly iNOS positive whereas specimens of bladder mucosa outside the malignant regions showed only a weak positive iNOS immunostaining. The endothelial cells in both normal urothlium and tumor tissue showed a highly positive eNOS immunostaining but its immunoreaction was not detected in either malignant or benign epithelium. MVD was (39.3?19.5)/HP and (29.3?10.5)/HP in iNOS positive and negative tissues respectively (P

[Objective] To observe imaging changes of Lumbar Intervertebral Disc Protrusion(LIDP)treated by Leverage Location manipulation.[Methods] 33 patients with lumbar intervertebral disc protrusion were observed(male=19,female=14),4 cases of expand type,25 cases of the outstanding type,4 cases of the free type.Using lever to observe the CT imaging of the cases.[Results]The 24 cases had no change in degree of Intervertebral Disc Protrusion while 9 cases had the changes.29 cases changed the compression relation between the nerve root and the lumbar intervertebral protrusion.Another 4 cases had nothing changed.[Conclusion] Leverage location manipulation has a little effect on changing the degree of the lumbar intervertebral protrusion,but can change compression relation between the nerve root and the lumbar intervertebral protrusion which proves that leverage location manipulation has effect on curing the LIDP.

AlM:To evaluate the value of color Doppler ultrasound in the diagnosis of traumatic retinal detachment.
METHODS: All 50 cases ( 56 eyes ) of suspicious traumatic retinal detachment from January to June in 2013 were enrolled in this study. All first received the two-dimensional ultrasound, then the color Doppler ultrasound, finally, these results were compared with the results of surgery.
RESULTS: All 56 eyes were observed intravitreal abnormal spots by the two-dimensional ultrasound, and 39 eyes were observed color blood signal by the color Doppler ultrasound. Forty eyes were approved retinal detachment by surgery.
CONCLUSlON: lt is better to display the intravitreal abnormal spots and color blood signal by the color Doppler ultrasound than by the two - dimensional ultrasound, so there is important clinical significance of the color Doppler ultrasound in the diagnosis of traumatic retinal detachment.

Objective To study the expression and clinical significance of BFGF factor(BFGF) and basic fibroblast growth factor receptor(BFGFR) in papillary thyroid carcinoma (PTC).Methods 80 casesof PTC and 10 cases of nodular goiter underwent surgical treatment from Mar.2001 to May.2005 in Shengjing Hospital of China Medical University.Samples were collected and immunohistochemistry staining was performed to detect the expression of BFGF and BFGFR.Results The expression rate of BFGF and BFGFR was 72.5％ (58/80)vs 70.0％ (56/80) in PTC,and 0.0％ (0/10) vs 0.0％ (0/10) in nodular goiter.The difference had statistical significance(P ＜0.05).The expression level of BFGF and BFGFR in PTC had no relation with sex,age or tumor size(P ＞0.05),but has relation with lymph node metastasis and UICC stage(P ＜0.05).The expression of BFGF was positively correlated with the expression of BFGFR (r =0.330,P =0.003).Conclusions BFGF and BFGFR are highly expressed in PTC but have no expression in nodular goiter or normal thyroid tissues.The expression of BFGF and BFGFR in PTC is closely related to lymph node metastasis and UICC stage,indicating the 2 may be involved in tumorigenesis,invasion,and metastasis.

Objective:To study if L-arginine cardioplegia can protect myocardium from ischemia/reperfusion injury. Method:Fourteen patients undergoing valve replacement were randomly divided into two groups:control group (crystal cardioplegia,C group),and test group(crystal cardioplegia+L-arginine,E group). Before aortic-clamping and after aortic unclamping,blood samples were taken to measure the concentrations of NO_2~-/NO_3~-, lactic acid (LA), malonylaldehyde (MDA), superoxide dismutase (SOD), xanthine oxidase (XOD). Immediately after aortic clamping and before aortic-unclamping, tissues were taken from the left atrial and examed with electron microscopy. Result: In C group NO_2~-/NO_3~- level decreased at aortic unclamping, 30 minutes later decreased significantly compared with that before aortic-clamping(P