Objective To explore the prevalence and epidemic factors of dental fluorosis among population in Ganhetan in-dustrial area.Methods The contents of fluoride in2392urine samples.20drinking water samples of centralized water supply systems,2spring water samples,12well water samples,18tea water samples,wheat,Chinese cabbage,soil and air samples were determined.The surveillene on prevalence of dental fluorosis among27225-60aged residents was conducted according to the unique national standard.Results The concentrations of fluoride in urine of2392residents[(1.91?1.39)mg /L]was high-er then that(about 0.5mg /L)in non-endemic fluoride poisoning area.The prevalence rate of dental fluorosis2722residents(86.4%)was higher than the rate(30%)ruled by Standards for Endemic Fluoride Poisoning Area Control.The contents of flu-oride were(1.39?0.08)mg /L for2well water samples,and0.05-0.30mg /L for the other32water samples.The contents of flu-oride in wheat,Chinese cabbage and soil didnt exceed the related standard s or background value.The concentration of fluoride in air samples[3.12-11.20?g /(dm 2 ?d)]exceeded the Grade II Standard[3.0?g /(dm 2 ?d)]ruled by Environment Air Quality Standard by0.04-2.73times.The contents of fluoride in tea water samples were0.22-2.96mg /L.Higher prevalence rate s of dental fluerosis were found in some counties with lower concentrations of fluoride in tea water samples.Conclusion Air pollu-tion by fluoride and drinking tea water containing higher concentration s of fluide might result in the higher prevalence of dental fluorosis among population in Ganhetan industrial area.

Objective To explore the prevalence of dental fluorosis among children in an industrial area of aluminum electrolysis. Methods 2 156 8-12-year old children were selected in a certain industrial area of aluminum electrolysis for investigation of their prevalence of dental fluorosis and the contents of fluoride in urine. At the same time, the contents of fluoride in the environmental media were investigated also. Results The prevalence of dental fluorosis was 51.11% for children, the content of fluoride in urine samples of children was (1.44

Objective To analyze the status of Huangshui River water pllution by chromium(Cr6+)and define the pollution source. Methods Water samples were collected from 5 monitoring points closely located at the bank of Huangshui River from the upper reaches to the lower reaches including A spring,Huanghai canal,Duoba water source well,Zhamalong,Xigangqiao,and also from the location of the upper reaches of Halejian River in the area of source of Huangshui River established as the control monitoring point during 1996-2003. The contents of Cr6+ in the water samples from above sampling points were determined. Results During 1999-2003,the average content of Cr6+ in the water samples from A spring was 45.192 mg/L,and those from Huanghai canal in the upper reaches of Huangshui River Zhamalong and Xigangqiao increased year by year gradually.By the end of 2003,Cr6+ hadn't been found in the water samples from the upper reaches of Halejian River,but Cr6+ had been found first in the water samples from Duoba water source well with a concentration of 0.007 mg/L. Conclusion Haibei Chemical Industrial Works near A spring was the main source for the Cr6+-pollution of water body of the upper reaches of Huangshui River.

ve To understand the impacts of industrial fluoride-induced pollution on environment and human health. Methods The contents of fluorides in environmental media, such as air, soil, vegetables, weeds, branches and leaves in area around a certain chemical factory (polluted area) were monitored. The prevalance of dental fluorosis, the contents of fluorides in hair and nail were investigated among 87 individuals without history of exposure to industrial fluorides, living in polluted area for more than 5 years, and 132 individuals in control area. Results Higher fluoride levels in environmental medias, higher prevalance rates of dental fluorosis, high contents of fluorides in hair and nails of population were found in polluted area compared with those in control area. Conclusion The in-dustrial fluoride-induced pollution from this chemical factory had resulted in the hazardous impacts on environmental quality and human health.

Objective To evaluate the preventive effect of BCG-PSN on respiratory infection of patients with chronic obstructive pulmonary disease.Methods 48 cases with COPD were randomly divided into two groups:the BCG-PSN group and the control group.The BCG-PSN group received BCG-PSN,0 5mg,im,twice a week for 18 times injection in addition to the routine therapy,and the control group only received routine therapy.Both groups were followed up every two weeks for six months.The serum IgA,IgG,IgM levels were determined before and 4,24 weeks after the treatment.Results Cases of infection and their lasting days,infective rate in the BCG-PSN group were significantly lower than that of the control group(P

Objective To evaluate the toxic effects of different concentrations of Coptidis Rhizoma extract on Caenorhabditis elegans. Methods Coptidis Rhizoma was extracted by water, and then selected some indicators to evaluate the toxic effects of Coptidis Rhizoma by using C. elegans as model organism. The effects of different concentrations of water extracts of Coptidis Rhizoma on lethality, maximum lifespan and median lethal time, individual development, spawning number and locomotion behavior were measured to evaluate the toxic effects on C. elegans. Results Compared with the control group, the lethality of C. elegans was significantly increased by water extracts of Coptidis Rhizoma at 0.5, 1.0, and 2.5 mg/mL (P < 0.01), and maximum lifespan and median lethal time were significantly decreased (P < 0.01). In the aspect of individual development, Coptidis Rhizoma extract at 1.0 and 2.5 mg/mL significantly inhibited the growth of C. elegans (P < 0.01). Three concentration extracts could significantly reduce the number of spawning in a certain dose-dependent manner in the aspect of reproductive behavior (P < 0.001), and the head swing frequency of C. elegans (P < 0.01) in the aspect of locomotion behavior, respectively. There was no significant difference in body bending frequency between the 0.5 and 1.0 mg/mL exposed group, of which the lowest bending frequency was 2.5 mg/mL exposed group (P < 0.01). There was no significant difference in the frequency of forward swing, backward swing and Omega/U swing of three exposed groups. Conclusion The water extracts of Coptidis Rhizoma had obvious toxic effects on C. elegans, which provided the basis for the biological evaluation of the toxicity of different processed products of Coptidis Rhizoma by using C. elegans as a model organism, and provided new ideas and methods for the biological assessment of toxicity of Chinese medicinal materials.

OBJECTIVE: To develop and validate an HPLC-MS/MS method for the measurement of 11 antipsychotics in human plasma samples for TDM(therapeutic drug monitoring) or other objectives. METHODS: Protein precipitation method with acetonitrile was used to extract the drugs in plasma. Agilent Poroshell 120 EC-C18 column(2.1 mm×50 mm, 2.7 μm)was used for chromatographic separation. The mobile phase was acetonitrile with 0.1% formic acid-water with 0.1% formic acid gradiently eluted at a flow rate of 0.3 mLmin-1. The injection volume was 5 μL. The deprotonated ions of analytes were ionized by electrospray(ESI) and detected in positive ionization by multiple reaction monitoring mode(MRM). RESULTS: The total run time of the chromatographic separation and mass detection was 6 min. Excellent linear relationship with correlation coefficient of 0.99 was obtained from 2 to 200 ngmL-1 for risperidone, paliperidone and olanzapine, from 10 to 1 000 ngmL-1 for aripiprazole, quetiapine, amisulpride, clozapine, chlorpromazine and sulpride, from 0.4 to 40 ngmL-1 for haloperidol, and 0.2 to 20 ngmL-1for perphenazine, respectively. The precision and accuracy varied from 2.7% to 10.8% and from 90.7% to 102.7%, respectively. CONCLUSION: This established method is simple, rapid, and economic, fulfilling all criteria for TDM, and could be successfully applied in the routine TDM of antipsychotics in clinic.

Objective To analyze the advantages of combined analysis of allergen skin prick test(SPT)and phadiatop/specific IgE antibody on the allergen diagnosis in asthmatic children.Methods Inhalant allergen SPT and Phadiatop test were done in 57 asthmatic children.Thirty-three cases of those asthmatic children were measured serum specific IgE antibody against dermatophagoids pteronyssinus.Results Dermatophagoids,molds and pets were the main inhalant allengens in asthmatic children.The positive rates of SPT and Pha-(diatop) in 57 asthmatic children were 86% and 79%,respectively,and the consistence rate between SPT and Phadiatop was 86%.Five cases with negative Phadiatop were confirmed to have molds allergy via SPT and molds specific IgE test.The consistence rate of dermatophagoids pteronyssinus SPT and specific IgE was 97%.Conclusion It is helped to improve the sensitivity and specificity of allergen diagnosis in asthmatic children when doctors combined analyze the results of allergen skin prick test and specific IgE test.

There has been a series of teaching innovation carried out to improve the clinical teaching of seven-year program students in the department of gynecology and obstetirics of Xinqiao hospital.By using case as a guide and problem as foundation,with multimedia and other advanced teaching plan,we paid more attention to the Chinese-English teaching to improve mixed diathesis of these medical students and find out a better teaching model.

Objective:To:investigate the curative effects and cost of sequential treatment of children communityacquired pneumoni(CAP) with azithromycin. Method:118 cases of CAP from this hospital were divided into two groups: sequential azithromycin therapeutic group (Group A) and iv ceftazidime group (Group B). Their clinical effect was observed and cost minimization analysis was carried out. Result:The total costs were 612?7. 3 yuan and 819?8. 2 yuan(P 0.05) in Group A and Group B respectively. Conclusion: The sequential azithromycin scheme is a better one to treat CAP.