Objective To investigate the clinical effect of edoravone combined with escin monosodium on the treatment of acute cerebral hemorrhage .Methods 60 patients suffered from acute cerebral hemorrhage were randomly divided into control group and observation group .Each group had 30 patients.The control group was conventionally treated by the internal medicine .On the basis of the research on the control group ,the observation group were treated by edoravone combined with escin monosodium intravenous drip .After the treatment of 14 days,28 days,the recovery of neural function,focus volume,clinical effect were evaluated.Results Comared with the control group,after the treatment of 14 days,28 days,NIHSS score was obviously lower than that of the control group ,the difference was sig-nificant (t=2.136,t=4.117,P<0.05,P<0.01).After the treatment of 14 days,hematoma and edema of the differences were not statistically significant (t=1.395,t=0.737,all P>0.05).After the treatment of 28 days,hema-toma and edema of the differences were statistically significant (t=3.140,t=2.367,P<0.01,P<0.05).The com-parison of the clinic effect of the two groups ,the total effect was statistically significant (χ2 =6.650,P <0.01). Conclusion Edaravone combined with escin monosodium on the treatment of cerebral hemorrhage can significantly improve neurologic impairment and cerebral hematoma .Clinical effect is definite and safe .

Osteoporosis is a major health concern and treatment of primary osteoporosis with anti-osteoporosis medications is needed to reduce fracture risk and burden. Before initiating anti-osteoporosis medications, secondary causes of osteoporosis should be considered and satisfactorily excluded. However, it can be challenging to differentiate primary osteoporosis from secondary osteoporosis, especially in patients with paucity of symptoms or who have less common clinical presentation. Hence, practical tips like Appropriate Care Guide on Osteoporosis forms the basis of initial secondary osteoporosis workup for primary care physicians. Snapshots of secondary osteoporosis are briefly discussed to facilitate “pattern recognition”.

Cannabinoid Receptor Modulators ; Endocannabinoids ; Humans ; Metabolic Syndrome ; metabolism

Objective To study the effects of staphylococcal enterotoxin B(SEB) on the proliferation of tumor infiltrating lymphocytes(TILs) from rectum adenocacinoma.Methods The TILs from patients with rectum adenocacinoma were stimulated with SEB and interleukin 2(IL-2) respectively,and then the proliferation of TILs,the secretion of IL-2 and tumor necrosis factor-?(TNF-?) were determined.Results SEB presented profound stimulating effect on the TILs from rectum adenocarcinoma both the proliferation of TILs and the secretion of cytokines.Compared with the IL-2,SEB stimulated TILs more quickly,and SEB acted more effectively in the early stage but weakly in the late stage.Conclution SEB was an effective TIL stimulator.

[Objective]To assess the protective effects of Xianlinggubao on articular cartilage and subchondral bone in experimental osteoarthritis induced by anterior cruciate ligament transaction (ACLT)in rabbits. [Methods]Thirty healthy Japanese white rabbits were divided randomly into three groups:sham group,ACLT+XLGB group and ACLT+ NS group. Rabbits in ACLT+XLGB group and ACLT+NS group received anterior cruciate ligament transection(ACLT)on the right knee. Rabbits in sham group received untreatment as control. ACLT+XLGB group received a daily administration of XLGB at a dose of 125 mg/kg of body weight for 6 weeks.In contrast,ACLT+NS group received saline at the same dose. All rabbits were sacrificed at 6 weeks after operation. The right femora were removed,measured for bone mineral density (BMD),and then were observed for the macro-pathologic changes with HE staining and graded by Mankin's scale. Expression levels of BMP-2 and MMP-13 were detected by immunostaining. The subchondral bone of right tibial plateau was cut and stained for the measurement of bone histomorphometry.[Results](1)The Mankin's score in ACLT+ XLGB group was significantly lower than that in ACLT+ NS group (P

Objective To establish a method for the fake indentification of Fengliaoxing Fengshi Dieda medicinal wines by TLC and GC. Methods The column was Supelco SE-30 (30 m?0.25 mm?0.25 ?m). The flow rate was 1.4 mL/min, and gas was N2. The inlets temperature was 210 ℃;the detectors temperature was 300 ℃;the column temperature use program rising temperature. Results TLC can identify whether ephedra and erycibe were contained in the medicinal wines. GC can identify whether diclofenac sodium was contained in the medicinal wines. Conclusion The method is accurate and reproducible, and can be used for the identification of Fengliaoxing Fengshi Dieda medicinal wines.

Objective:To explore the central mechanism of working memory impairments experienced by patients with schizophrenia.Methods:Using block-design,ten normal and twenty patients with schizophrenia perform digital recognition(remember two or five digitals simultaneous and make judgment about whether the digital presented latter is that appeared before)and direction judgment of arrows(toward to left or right)while proceeding blood-oxygen level dependent contrast functional magnetic resonance imaging(BOLD-fMRI).Results:On the condition of same cognitive load,abnormal activation of basal ganglia occurred in schizophrenics.Comparing to normal control,patients with schizophrenia exhibited a tendency of more activation in dorsolateral prefrontal cortex.Conclusion:Abnormal activity of basal ganglia and dorsolateral prefrontal cortex may be the critical cause of working memory deficit experienced by patients with schizophrenia.

Objective To systematically evaluate the safety of laparoscopic distal pancreatectomy (LDP) compared with open distal pancreatectomy (ODP).Methods Databases including Cochrane library,MEDLINE,EMbase,Google Scholar and Chinese National Knowledge Infrastructure were searched to enroll randomized clinical trials (RCT),controlled clinical trials (CCT) or retrospective case-control studies to compare LDP with ODP.All articles received quality assessment according to the inclusion and exclusion criteria,then the selected indices were analyzed using the Review Manager Version 5.0 software (The Cochrane Collaboration,Oxford,United Kingdom).Results 21 manuscripts with a total of 2 797 patients were enrolled.1 150 patients underwent LDP and the remaining 1 647 patients underwent ODP.In 20 studies (n =2 597),the total postoperative complication rates were 33.90％ for the LDP group versus 46.80％ for the ODP group [RR =0.76,95％ CI(0.69 ～ 0.84),P ＜ 0.01].In 8 studies (n =1 869) there was no significant difference [RR =0.51,95％ CI(0.21 ～ 1.24),P ＞0.05] in the perioperative mortality between LDP (4/703) and ODP (18/1 166).In 20 studies (n =2 757) there was no significant difference [RR =0.89,95％ CI(0.75 ～ 1.06),P ＞ 0.05] in the pancreatic fistula rate between LDP (168/1 132) and ODP (281/1 625).In 11 studies (n =1 840) the wound infection rate of LDP (3.24％) was significantly lower than ODP (10.85％) [RR =0.34,95％ CI(0.23 ～ 0.52),P ＜ 0.01].No significance was found between the two groups in the rates of pulmonary complications,peritoneal infection,urinary tract infection,postoperative bleeding,pseudocyst formation,intestinal obstruction and ascites formation between LDP and ODP.Conclusions When compared with the traditional open procedure,LDP has the advantages of significantlylower rates of postoperative complication and wound infection.There were no significant differences in postoperative mortality,and pancreatic fistula rate between LDP and ODP.This meta-analysis suggests that LDP is a safe and feasible operative method.

BACKGROUND:Hypoxic death limits application of cells in transplantation and tissue regeneration.
OBJECTIVE:To investigate the protective effects of mangiferin on bone marrow-derived mesenchymal stem cells against hypoxia injury-induced apoptosis resulted from cobalt chloride.
METHODS:Rat bone marrow-derived mesenchymal stem cells were in vitro cultured and hypoxia cellmodel was established by cobalt chloride. Model cells were treated with mangiferin. Protective effects of mangiferin were detected using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide;cellapoptosis and mitochondrial membrane potential were detected using flow cytometry.
RESULTS AND CONCLUSION:Cobalt chloride significantly inhibited growth of bone marrow-derived mesenchymal stem cells in a dose-dependent manner. The apoptosis rate of cells was (42.49±3.96)%after treated with 200μmol/L cobalt chloride for 12 hours, (46.37±4.49)%after treated for 24 hours. With increasing concentration of mangiferin, apoptosis of bone marrow-derived mesenchymal stem cells in hypoxic model was gradual y reduced (P<0.01), indicating that mangiferin has a protective effect in a concentration-dependent manner on rat bone marrow-derived mesenchymal stem cells in hypoxic injury. Cobalt chloride can induce hypoxic model successful y in bone marrow-derived mesenchymal stem cells. There are some advantages of accurate dose control, no special equipment requirements, and easy operation. Mangiferin can effectively inhibit bone marrow-derived mesenchymal stem cells apoptosis under hypoxic injury.