Objective To observe the effect of shuanghuang liquid on bum wound healing. Meth-ods 120 cases of patients with moderate bums adopted shuanghuang liquid (the treatment group) and gentamyein (the control group) for wound dressing change, wound pain and wound healing effect were com-pared between the two groups. Results The two groups showed difference in reducing wound pain and shortening time of wound healing. Conclusions Shuanghuang liquid was superior to gentamycin in wound healing and wounds pain.

Objective To analyse effect of tripterygium glycosides on serum ion and β2-GPI/ox-LDL in patients with IgA nephropathy. Methods 54 patients who were diagnosed with primary IgA nephropathy in our hospital were collected.All patients were randomly divided into experimental group and control group, 27 cases in each group.Control group was given conventional Benazepri1 Hydrochloride Tablets 10 mg, 1 times per day orally;given Dipyridamole Tablets 50 mg, 3 times per day orally, patients in experimental group were given tripterygium glycosides 20 mg on the basis of control group treatment orally,3 times per day.All the patients were treated for 6 month.After the treatment, the serum levels of Ca,P,β2-GPI/ox-LDL and renal function related index were detected in all patients.Results Compared with control group post-treatment, the serum level of Ca was higher(P<0.05); and the serum level of P was lower in experimental group(P<0.05); the serum level of β2-GPI/ox-LDL was lower in experimental group(P<0.05);the serum levels of Scr and BUN were lower in experimental group(P<0.05).Conclusion The tripterygium glycosides can significantly reduce the serum levels of P,β2-GPI/ox-LDL,Scr and BUN,and increase serum Ca level in patients with IgA nephropathy, improve renal function.

Objective To establish osteosarcoma vaccine by the LM9 osteosarcoma derived from C3h mice hybridized with activated B lymphocytes and study its biological behavior and the antitumor efficacy. Methods The LM9 osteosarcoma derived from C3h mice was fused with LPS activated B lymphocytes by using 50%PEG. The fused cells was selected by HAT medium and cultured in vitro, and the biocharacter and efficacy of the fusion vaccine were investigated. Results In contrast to LM8, the fused cells grew significantly slowly in vitro. All the mice under the protection of fused vaccine survived without tumor (8/8), while all the mice in the control group succumbed to the tumor with no survival (8/8), 75%of the mice inoculated subcutaneously with cell fusion vaccine survived without tumor burden after implantation of LM8 cells subcutaneously. The mice in the control group developed tumors and died within 45 days without any exception. Conclusion It is possible that the LM9 osteosarcoma biology characteristics change after fused with LPS activated B lymphocytes. Cell fusion osteosarcoma vaccine could produce prophylactic and therapeutic effects in mice.

OBJECTIVE:To establish the method for assaying bacterial endotoxins in levofloxacin hydrochloride injec?tion.METHODS:The interference test of3batches of levofloxacin hydrochloride injection with2kinds of limulus test agent was studied.RESULTS:The interference between the samples and limulus test agents was eliminable(below1.0mg/ml).The detection results were up to standard.CONCLUSION:The bacterial endotoxin in the sample can be examined by limulus test instead of pyrogen test in rabbits.

Electroencephalography ; Epilepsies, Myoclonic ; diagnosis ; therapy ; Female ; Follow-Up Studies ; Humans ; Infant ; Male ; Prognosis ; Spasms, Infantile ; diagnosis ; Time Factors

BACKGROUND: In recent years, acel ular human cadaveric dermis has been applied for repairing dermal defects and fil ing soft tissues, but this repair material is expensive and difficult to obtain. OBJECTIVE: To explore the repair effect of tissue-engineered acel ular dermis on wound healing of skin ulcer. METHODS: Sprague-Dawley rats were randomly divided into autologous reticular dermis and acel ular dermis groups. The tissue-engineered acel ular dermis was prepared by hypotonic solution, 2.5 g/L trypsin, 0.5% Triton X-100 and PBS solution, and in the meanwhile, a rat model of skin ulcer was established. Then, autologous skin graft and tissue-engineered acel ular dermis were transplanted into the autologous reticular dermis group and acel ular dermal group, respectively. Subsequently, comparative study about the repair effect and relative mechanism between two groups was performed. RESULTS AND CONCLUSION: The tissue-engineered acel ular dermis showed a white and grainy shape at room temperature. And hematoxylin-eosin staining showed that: the internal structure of tissue-engineered acel ular dermis exhibited a dendritic distribution, and the gap between col agen fiber bundles was relatively large. At 2, 3, 4 and 8 weeks after dermal transplantation, the survival rate of skin graft in the acel ular dermis group was significantly higher than that in the autologous reticular dermis group (P < 0.05). And the wound contraction rate in the acel ular dermis group was significantly higher than that of the autologous reticular dermis group at 3, 4 and 8 weeks after dermal transplantation (P < 0.05). In addition, the average diameter and clearance rate of col agen fibers in the normal skin around wound in the acel ular dermis group were significantly higher than those of autologous reticular dermis group (P < 0.05). To conclude, it is relatively simple to prepare tissue-engineered acel ular dermis under mild conditions, and the prepared tissue-engineered acel ular dermis presents a regular shape. Moreover, this acel ular dermis achieves desired outcomes in repairing dermal defects, which can promote wound healing by reducing the intradermal DNA residual and significantly improving some biomechanical properties in vivo.

To establish a xenografted tumor strain of human osteosarcoma in nude mice and a homologous cell line, the osteosarcoma speciments from patients were inoculated into the subcutaneous tissue of nude mice. After the transplanted tumor had grown to about 2cm in diameter, the tumor tissue was cultured in vitro and the continuously growing cells were analyzed by morphology, histochemistry, immunohistochemical straining, cell cycling analysis ,chromosome analysis and heterotransplantation. A newly established cell line designated PL 1 was thus obtained in this laboratory in continuous culture for over 100 passages. Under light and electron microscopes, the cells assumed the main characters of malignancy.Morphological observation, histochemistry analysis and BMP immunohisto chemical straining showed that they had the features of osteosarcoma.The cell cycle analysis showed 53 2% of the cells were in G 1. This cell line could produce ALP and BMP.Chromosome analysis confirmed that they had retained a karyotype of human cancer cells and a hypotriploid feature with a mode number of around 64～66.The tumor formation rate after heterotransplantation was 100%. It had lung metastasis characters. Therefore, PL 1 cell line derived from the xenograft in nude mice has been established and maintained for over 9 months trough 100 passages, and this cell line can provide material and model for further investigation of human osteosarcoma.

Objective To investigate clinical outcomes and side effects of 103Pd seed brachytherapy for malignant tumors. Methods Twenty patients with residual or recurrent unresectable malignancies were treated with 103Pd seed implantation under the guidance of ultrasonigraphy or CT scans. Three patients were given a local anesthesia and 17 patients, general anesthesia. The match peripheral doses ranged from 97.3 Gy to 182.78 Gy (mean, 123 Gy). The activity of each seed ranged from 1.4 mCi to 1.8 mCi. The planning target volume (PTV) included a 1 cm isotropic expansion margin around the clinical target volume (CTV). The seeds were retrogradely placed with a Mick applicator. External beam radiation was required 3～4 weeks after seed implantation in 6 patients, with a total dose of 45～50 Gy and 2 Gy each fraction. All of the patients received CT scanning after implantation for quality evaluation and underwent routine chest X-ray examination at 24～48 hours for seed observation. Results A complete response was achieved in 5 patients and a partial response in 12 patients. Two patients were assessed as having stable disease. In 1 patient with prostatic cancer, the serum PSA level was decreased significantly. The local control rate was 90% (18/20). The 20 patients were followed for 2～25 months (median,11 months).Two patients were lost to follow-up at 6 and 12 months after operation, respectively. Twelve patients died and 6 patients survived.No severe complications were recorded postoperatively. Conclusions 103Pd brachytherapy for malignant tumors gives a high local control rate and satisfactory reliability.

Objective To study the clinical application oflaparoscopic-assisted operation to colorectal neoplasms. Methods 28 cases of colorectal neoplasms underwent laparoscopic surgery from July 1997 to November 2000. The mean age was 65.3 years old(33～89)years.3 cases underwent right hemicolectomy.1 case of sigmoid colon adenoma was given partial colectomy. Sigmoid-rectal anterior resection was conducted in 21 cases. 3 cases underwent abdominal peritonieal resection. Results 5 cases were converted to open laparotomy.The mean operative time was 178(150～300)min for 23 cases given laparoscopic surgery with 135(30～1000)ml of average intraoperative bleeding. Neither postoperative complications nor intraoperative deaths occurred.1 case of low portion rectal cancer showed abdominal metastasis 12 months after surgery.Neither port site nor incision metastasis happened. Conclusions Laparoscopic assisted surgery has the advantages of less surgical trauma,less gastrointestinal interference and quicker recovery. Under the circumstances of radical resection and selected candidate,laparoscopic assisted surgery can be applied to colorectal neoplasms.

Objective To study on the diagnosis methodology of breast cancer using integrated analysis of proteomics and metabonomics approach.Methods The serum samples from the 14 breast cancer patients and 7 healthy people were analyzed by two-dimensional electrophoresis in order, to find differential expressed proteins. On the other hand, an HPLC-based urinary metabonomics profiling was employed to pick out chemical patterns between breast cancer patients and healthy people.Results Glutathione S-transferase M5 was found highly expressed in the breast cancer patients′serum.The concentration pattern of S-Adenosylmethionine, Orotidine, 1-Methyladenosine and N2-Methylguanosine in patients′ urine were peculiarly different from that in the healthy people.Conclusion There are peculiar chemical pattern differentiae between the body fluid of breast cancer patients and the body fluid of healthy people. Synthetic study of cancer proteomics and metabonomics can contribute not only to prognosis of cancer but also to the pathogenesis of cancer.