Objective　To monitor KBD prevalence rate in Changdu of Tibet.Methods　The aged 7～12 year children are tested with X- ray and epidemiological investigation.Results　Xizang are still high yet,the 3 of 4 porints X-rates are more than 20% and the highest one is 55.34%.Conclusions　The Changdu is the severest point of KBD in our country.

In order to investigate the affinity of aptamers to Bacillus anthracis spore, a custom synthesized 78 mer random DNA library was subjected to 15 rounds of selection against spores of vaccine strain A.16R by using SELEX method. The selected aptamers were cloned and sequenced. Macaw 2.05 and DNAsis 2.5 package were employed to analyze the conserved sequences and second structure of the aptamers, respectively. Affinities of aptamers to the spores were visualized by biotin streptavidin horseradish peroxidase system. The results showed that affinities of the aptamers were different. The highest OD at 450nm was 1.2, and the lowest was 0.25. The second structure analysis revealed possible stem loops for binding to the spores. The conserved sequences, AGGGG, CCCCG, GGGTT and ACACT, were found and the aptamers having same conserved sequence demonstrated similar affinity to the spores.

Objective To Observe the effects of tumor necrosis factor ? (TNF ?) on cell proliferation and Intracellular free calcium concentraction ([Ca 2+ ]i) in endothelium of human umbilical vein endothelial cells (HUVEC) and investigate the pathogenesis of pregnancy induced hypertension syndrome (PIH). Methods Confluent monolayer of HUVEC was directly incubated with TNF ? at following final concentrations: 500, 1 000, 2 000 U/ml for 24 hours. The percentages of different cellcycles and [Ca 2+ ]i were measured by flow cytometry and fluorospectrophotometry. Results Incubated with TNF ?, the endothelial cells were elongated and transformed into fibroblast like cells. Border of nucleus was sharp, clarity, and cells were in regular shape. But there were abnormal granules in cytoplasma and some cells detached at the concentrotion of 2 000 U/ml of TNF ?. Stimulated by TNF ?, the percentage of cellcycles from phase G 0+G 1 to S and G 2+M decreased significantly and it was dose dependent. [Ca 2+ ]i increased significantly and dose dependent as well. Conclusion TNF ? may injure endothelium directly and inhibit its proliferation and repair through the changes of [Ca 2+ ]i level. It may play an important role in the pathogenesis of PIH.

This paper described the existing problems of transformation benefit from a target,two ideas,three questions,four reasons,and five measures.Through establishing clear objectives,combing the status of the achievement trasformation some problems were raised.Also,it Was analyzed for the reasons of deficient transformation benefit and suggested the taken measures.

Objective To study the expression of RhoA protein in colorectal carcinoma. Methods The expression of RhoA protein in 92 cases of colorectal ccarcinoma and 30 cases of normal rectal tissue was detected by SP immunohistochemical technique. Results RhoA protein were negative in 40 cases of normal colorectal tissue. In 92 cases of colorectal carcinoma, the positive rate of RhoA protein was 70.7%. It was significantly higher than that in the normal colorectal tissue. The positive rate of RhoA protein in high-differentiated 、moderate-differentiated and lowdifferentiated rectal carcinoma were 43.5% 、74.4% and 86.7% ,respectively(P ＜0.05). The positive rates of RhoA in phase Ⅰ、phase Ⅱ、phase Ⅲ and phase Ⅳ were 48.6% 、77.3% 、83.3% and 94.1%, respectively (P ＜ 0.05).The positive rate of RhoA protein in patients with lymphnode mestastasis and non-lymphnode mestastasis were 82% and 57.1% (P ＜ 0.05). Conclusion RhoA protein could be related with the differentiation、invasion、metastatsis and prognosis of colorectal carcinoma.

Adolescent ; Adult ; Child ; Child, Preschool ; Female ; Graft vs Host Disease ; prevention & control ; Hematopoietic Stem Cell Transplantation ; methods ; Humans ; Male ; Middle Aged ; Nutritional Status ; Nutritional Support ; Young Adult

BACKGROUND: Proximal femoral anti-rotation intramedullary nail for treating intertrochanteric fracture is widely used in clinical practice, but there are still some cases of failure of internal fixation after operation, and osteoporosis of proximal femur is considered as an important reason. Singh index is an important index to evaluate the severity of osteoporosis in the proximal femur. Based on the Singh index, it is of great significance to explore the effect of different degrees of osteoporosis on the treatment of intertrochanteric fracture with anti-rotation intramedullary nail in the proximal femur, so as to reduce the failure rate of internal fixation and increase the success rate of operation. OBJECTIVE: To explore the effect of different osteoporosis on the treatment of intertrochanteric fracture with proximal femoral anti-rotation intramedullary nail, so as to provide new ideas and experimental basis for clinical treatment of intertrochanteric fracture. METHODS: CT data of one patient with intertrochanteric fracture of the left femur were imported into Mimics 19.0 and Geomagic studio 2017 software to extract and optimize the three-dimensional model of the right femur. SolidWorks 2017 software was used to draw the internal fixation model and assemble it with the femur model according to the standard operation technology, and import it into HyperMesh 14.0 software in STEP format to cut the bone according to AO-2.1 type of intertrochanteric fracture. Trabecular bone based on Singh index 1-6 was established to obtain six models of A-F. Material property parameters, boundary conditions and applied loads were set. Finally, the data were stored as K files and imported into LS-DYNA software for solution. RESULTS AND CONCLUSION: (1) When the femoral head was stressed, the spiral blade in the bone block of the femoral head and neck of Singh 6-Singh 1 was cut, the common bone trabecula disappeared, the stress bone trabecula wrapped with the spiral blade did not disappear, but loaded with certain stress, so that the spiral blade still had a large contact area and holding force, maintained the reduction of the fracture, and reduced the varus and rotation of the bone block of the femoral head and neck. (2) From Singh 6-Singh 1, with the disappearance of stress bone trabecula, the more serious osteoporosis is, the more likely failure will be in the treatment of intertrochanteric fracture with proximal femoral anti-rotation intramedullary nail. (3) The spongy bone trabeculae, especially the stress bone trabeculae, play an important role in maintaining the elastic stability of the proximal femur by resisting and buffering the bending strain.

·AIM: To investigate the efficacy and security of the silicone membrane implant through observing intraocular pressure, filtering blebs and histopathologic results.·METHODS; Forty rabbits were randomly divided into 4 groups. Each group consisted of 10 rabbits. Trabecu-lectomy and silicone membrane implantation were performed in one eye of each rabbit, while the other eye was only performed trabeculectomy as control. The change of intraocular pressure, filtering blebs were observed postoperatively. And each eye had undergone histopathologic examination.·RESULTS:The duration of low intraocular pressure and existence of filtering blebs in implanted eyes was longer than that in controlled eyes. Light microscopy revealed that patent drainage tract and biting site could be seen in silicone membrane implanted eye. The activity and regularity of fibroblast proliferation in implanted eyes and controlled eyes were similar. Excessive expression of fibroblast proliferation was not induced by silicone membrane.·CONCLUSION: Characterized by safety, effectiveness and simplicity, silicone membrane implantation can act as a new drainage surgery.KEYWORDS: silicone membrane; drainage surgery;intraocular pressure

Abstract?AlM:To compare the effects of different adjustable suture in glaucoma trabeculectomy on early tear film function.?METHODS:Sixty-eight cases of primary glaucoma ( 76 eyes) during January 2012 to June 2014 in our hospital were selected and divided into exposure conjunctival suture group ( 34 cases, 36 eyes ) and embedded conjunctival suture group ( 34 cases, 40 eyes ) according to treatment. Adjustable suture exposed conjunctival suture and embedding conjunctival suture was given to two groups, respectively. lntraocular pressure ( lOP ) before and after treatment 7, 14, 30d were observed and Schirmer test, tear break-up time, No Hikaru sensitivity and the occurrence of adverse reactions after treatment 1, 30d were recorded.?RESULTS: After the treatment, the mean lOP of two groups were decreased significantly ( P < 0. 05 ). The average lOP after treatment of 1d in the two groups were not statistically different (P>0. 05), after treatment 7, 14, 30d embedded conjunctival suture group was significantly higher than that of exposure conjunctival suture group ( P<0. 05). After 1d of treatment, Schirmer test, tear break-up time, No Hikaru sensitivity of two groups compared no significant difference (P<0. 05). After treatment 7, 14, 30d embedded conjunctival suture group Schirmer test, tear break- up time, was significantly superior to expose conjunctiva suture group (P<0. 05). The 30d package after treatment of conjunctival suture group buried adverse reaction rate was significantly lower than that of exposed conjunctival suture group (P<0. 05).? CONCLUSlON: Trabeculectomy operation with adjustable thread embedding conjunctival suture has few effects on the tear film function in patients with early postoperative complications, lower, and operation effect is better than that of exposed conjunctival suture.