2.Thinking of improvement of transformation benefit in military scientific research achievements
Pei ZHEN ; Yibo LIU ; Decheng MA
Chinese Journal of Medical Science Research Management 2008;21(6):353-355
This paper described the existing problems of transformation benefit from a target,two ideas,three questions,four reasons,and five measures.Through establishing clear objectives,combing the status of the achievement trasformation some problems were raised.Also,it Was analyzed for the reasons of deficient transformation benefit and suggested the taken measures.
4.STRUCTURES AND AFFINITY ANALYSIS OF APTAMERS TO BACILLUS ANTHRACIS SPORES
Pei ZHEN ; Yajun SONG ; Ji WANG
Medical Journal of Chinese People's Liberation Army 1981;0(06):-
In order to investigate the affinity of aptamers to Bacillus anthracis spore, a custom synthesized 78 mer random DNA library was subjected to 15 rounds of selection against spores of vaccine strain A.16R by using SELEX method. The selected aptamers were cloned and sequenced. Macaw 2.05 and DNAsis 2.5 package were employed to analyze the conserved sequences and second structure of the aptamers, respectively. Affinities of aptamers to the spores were visualized by biotin streptavidin horseradish peroxidase system. The results showed that affinities of the aptamers were different. The highest OD at 450nm was 1.2, and the lowest was 0.25. The second structure analysis revealed possible stem loops for binding to the spores. The conserved sequences, AGGGG, CCCCG, GGGTT and ACACT, were found and the aptamers having same conserved sequence demonstrated similar affinity to the spores.
5.The Expression and clinical Significance of RhoA protein in Colorectal Carcinoma
Zhen YANG ; Xinhong PEI ; Shishi QIAO
Chinese Journal of Primary Medicine and Pharmacy 2010;17(17):2330-2331
Objective To study the expression of RhoA protein in colorectal carcinoma. Methods The expression of RhoA protein in 92 cases of colorectal ccarcinoma and 30 cases of normal rectal tissue was detected by SP immunohistochemical technique. Results RhoA protein were negative in 40 cases of normal colorectal tissue. In 92 cases of colorectal carcinoma, the positive rate of RhoA protein was 70.7%. It was significantly higher than that in the normal colorectal tissue. The positive rate of RhoA protein in high-differentiated 、moderate-differentiated and lowdifferentiated rectal carcinoma were 43.5% 、74.4% and 86.7% ,respectively(P <0.05). The positive rates of RhoA in phase Ⅰ、phase Ⅱ、phase Ⅲ and phase Ⅳ were 48.6% 、77.3% 、83.3% and 94.1%, respectively (P < 0.05).The positive rate of RhoA protein in patients with lymphnode mestastasis and non-lymphnode mestastasis were 82% and 57.1% (P < 0.05). Conclusion RhoA protein could be related with the differentiation、invasion、metastatsis and prognosis of colorectal carcinoma.
6.Effects of Tumor Necrosis Factor-? on Proliferation and Intracellular Free Calcium Concentration in Endothelium of Human Umbilical Vein Endothelial Cells
Zhen JIN ; Ling PEI ; Dezhi WANG
Chinese Journal of Obstetrics and Gynecology 2000;0(11):-
Objective To Observe the effects of tumor necrosis factor ? (TNF ?) on cell proliferation and Intracellular free calcium concentraction ([Ca 2+ ]i) in endothelium of human umbilical vein endothelial cells (HUVEC) and investigate the pathogenesis of pregnancy induced hypertension syndrome (PIH). Methods Confluent monolayer of HUVEC was directly incubated with TNF ? at following final concentrations: 500, 1 000, 2 000 U/ml for 24 hours. The percentages of different cellcycles and [Ca 2+ ]i were measured by flow cytometry and fluorospectrophotometry. Results Incubated with TNF ?, the endothelial cells were elongated and transformed into fibroblast like cells. Border of nucleus was sharp, clarity, and cells were in regular shape. But there were abnormal granules in cytoplasma and some cells detached at the concentrotion of 2 000 U/ml of TNF ?. Stimulated by TNF ?, the percentage of cellcycles from phase G 0+G 1 to S and G 2+M decreased significantly and it was dose dependent. [Ca 2+ ]i increased significantly and dose dependent as well. Conclusion TNF ? may injure endothelium directly and inhibit its proliferation and repair through the changes of [Ca 2+ ]i level. It may play an important role in the pathogenesis of PIH.
7.The monitor report of Kashin-Beck Disease prevalence ra te in Changdu of Tibet
Jiang, LI ; Zhen-Qun-Pei, DING
Chinese Journal of Endemiology 2001;20(2):130-131
Objective To monitor KBD prevalence rate in Changdu of Tibet.Methods The aged 7~12 year children are tested with X- ray and epidemiological investigation.Results Xizang are still high yet,the 3 of 4 porints X-rates are more than 20% and the highest one is 55.34%.Conclusions The Changdu is the severest point of KBD in our country.
8.How to train creative researchers in proteomics: our experience
Xue GAO ; Xueli ZHANG ; Xiaohong QIAN ; Pei ZHEN
Chinese Journal of Medical Science Research Management 2014;27(2):184-186
We here systematically summarize our practice and its effectiveness in the creation and development of the discipline proteomics in Academy of Military Medical Sciences,and the establishment of a system for identifying and training talented researchers in proteomics.The methods we used to train the researchers and to develop a vigorous team are discussed.These practices contributed much to the development of proteomics in China.
9.Effectiveness of electroacupuncture combined with rehabilitation exercises on swallowing function of dysphagic stroke patients
Zhen HUANG ; Hai-Xia YAN ; Yu MIN ; Pei-Shun CHEN ;
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(07):-
Objective To observe the therapeutic effect of electroacupuncture combined with rehabilitation exercises on swallowing function and cerebral perfusion in dysphagic stroke patients.Methods Sixty-two stroke pa- tients with dysphagia were randomly divided into a treatment group(n=32)and a control group(n=30).The treat- ment group received electroacupunture,rehabilitation exercise and conventional medical treatment,while the control group received only rehabilitation exercise and conventional medical treatment.They were treated once a day,6 times a week for 4 weeks.Water drinking test,stethocatharsis scores and swallowing fluorography were used to assess the swallowing function before and after treatment.Single photon emission computed tomography(SPECT)was also em- ployed to observe the status of cerebral perfusion before and after treatment.Results It was shown that the swallo- wing function and cerebral perfusion in the treatment group were significantly better than the control group after treat- ment.The effective rate in the treatment group was 96.88% while that of the control group was 66.67%.Conclu- sion Electroacupuncture combined with rehabilitation exercises is effective in treating the dysphagic stroke patients, and can significantly improve the brain perfusion of these patients.
10.Relationship between CIDE-B change and neuronal apoptosis under various injuring conditions
Hong-Zhen SUN ; Zheng-Guo WANG ; Pei-Fang ZHU ;
Chinese Journal of Trauma 2003;0(07):-
Objective To investigate the relationship between CIDE-B change and apoptosis by detecting expression of CIDE-B gene and protein under various injuring conditions(firearm injury and in- cision injury)and comparing neuronal apoptosis.Methods A total of 108 New Zealand rabbits were randomized into firearm injury group,incision injury group and control group.Samples were harvested at different time points after injury for measuring CIDE-B mRNA expression level by RT-PCR,demonstrating morphological distribution of CIDE-B mRNA by in situ hybridization and determining CIDE-B protein level by Western blot.Results Following nerve injury,CIDE-B mRNA expression in spinal cord was en- hanced markedly in the firearm injury group,increased from at day 1,reached peak at day 7,lasted for two weeks and declined after four weeks.CIDE-B mRNA expression was increased late,with small range in the incision injury group.The changes in CIDE-B protein level accorded with those in CIDE-B mRNA level.There was slight CIDE-B mRNA expression,distributing in spinal cord neuronal cytoplasma,in spinal cord tissues in the control group.Conclusions Following peripheral nerve injury,expressions of CIDE-B mRNA and protein are enhanced in spinal cord neurons and distributed in the grey matter of spi- nal cord.Such enhancement is more marked after firearm injury than incision injury.