Superantigens are potent activators of T cells,causing rapid and massive proliferation of T cells and superantigen-dependent-cell-mediated cytotoxicity with extremely low doses.However such antitumor effects lack selectivity.Thus with the methods including monoclonal antibody targeting or binding superantigens to the surface of tumor cells as well as genetic engineering,scientists have done lots of work on targeted therapy of malignancies with superantigen.

Adolescent ; Adult ; Diverticulum ; diagnostic imaging ; Female ; Humans ; Jugular Veins ; diagnostic imaging ; Male ; Middle Aged ; Tomography, X-Ray Computed ; Young Adult

Objective To explore the corresponding relationship between isolated Q waves in lead Ⅲ on the electrocardiography(ECG) and echocardiographie segmental diagnosis of myocardial infarction(MI).Methods The shape magnitude of Q waves of 27 patients with MI and the relationship with eehocardiographie features were investigated. Results Twenty-four patients with inferior wall myocardial infarction had close relationship with isolated Q waves in lead Ⅲ on the ECG. Conclusions There is definite corresponding relation between isolated Q waves in lead Ⅲ and inferior wall myocardial infarction.

Adult ; Aged ; Angiomyolipoma ; diagnosis ; diagnostic imaging ; Carcinoma, Renal Cell ; diagnosis ; diagnostic imaging ; Diagnosis, Differential ; Female ; Fibroma ; diagnosis ; diagnostic imaging ; Humans ; Kidney Neoplasms ; diagnosis ; diagnostic imaging ; Leiomyosarcoma ; diagnosis ; diagnostic imaging ; Male ; Mesenchymoma ; diagnosis ; diagnostic imaging ; Middle Aged ; Tomography, Spiral Computed ; methods

Aim To investigate glutamate-induced [ Ca2 + ] i changes in cultured rat neonatal cortical astrocytes after hypoxia/reoxygenation, H2O2 or high concentration of L-glutamate injury. In the meantime, the effects of Gingko biloba extract (GbE) were examined. Methods [ Ca2+ ]i changes in astrocytes were monitored by laser scanning confocal microscopy with the Ca2+ sensitive fluorescent probe [ Ca2 + ] i, but decrease by ( 3.3 ± 1.6) %, (81 ± 11 ) % and ( 81 ± 7 ) %, respectively. Pretreatment with H2O2 or high concentration of L-glutamate injury were ( 135 ± 98 ) %, ( 117 ± 93 ) % and ( 89 ± 36) %,different injury. Conclusion Hypoxia/reoxygenation, H2O2 and high concentration of L-glutamate impaired astrocytes' response to exogenous L-glutamate, and then bidirectional communication between astrocytes and neurons could not take place. GbE could improve the abnormal responses and maintain the normal function of astroglical network. These effects support that GbE has potential beneficial actions against brain injury.

Medical imaging involves not only the multidisciplinary knowledge,but also the concurrent updating of the knowledge system related to the imaging technology development.In this case,it's an important issue how to guarantee the teaching quality of the medical imaging postgraduate education effectively and cultivate the professsionals with higher comprehensive quality in the limited teaching time available.Thus the implementation as well as the teaching effects of the reading report in the promotion of medical imaging postgraduates' research ability and comprehensive quality is needed to be discussed from the accumulation and update of knowledge system.

The microvasculature of the lung of three humans was studied with the replicascanning electron microscopic method. The pulmonary arteriole belongs to microvasculature at the level of the respiratory bronchiole and gives out some side branches to the alveoli. The diameter of the arteriole is about 30-50 ?m. The arteriole divided into terminal arterioles, which accompanied with primary alveolar ducts. The diameter of the terminal arteriole is about 10-20 ?m, 17.35?1.62 ?m in average (n=30). There are obvious imprints of smooth muscles and endothelial nuclei on the surface of the casts of arteriole and terminal arteriole. The diameter of the alveolar capillary is 5.87?0.90 ?m in average (n=500). The proportion of the capillary area arround the alveole is 66?1.4%. There are two patterns of capillary network, i. e. long mesh and round mesh in type. The diameter of the mesh is (9.24?2.02)?(5.65?1.40) and 5.75?0.84 ?m, respectively. The distance of the mesh center is 17.13?1.74 ?m (long) and 11.52?1.12 ?m (short) in long mesh, and 11.63?0.88 ?m in round mesh. The capillary network of the long mesh located at the entrance and the base of the alveoli; and the round mesh in the area between them. The metaarteriole supplying the alveoli enters the alveoli at their entrance and thev venule draining the alveoli at their base. The distance between them is about 330 ?m.According to the microvascular architecture, the pulmonary microvasculature may be divided into three ordered units: i. e. the alveolar, the alveolar duct, and the respiratory bronchiolar units.

Objective To construct eukaryotic expression bicistron plasmid vector pcDNA3.1-hOPG,and to detect the expression of the plasmid in vitro.Methods hOPG cDNA fragment was extracted from plasmid MGC:29565,amplified by PCR,and inserted into pcDNA3.1(-)vector,then identified by restriction endonuclease digestion and nucleotide sequencing.C2C12 cells were transfected with the plasmid using Lipofectamine 2000.The expression of OPG protein was detected by immunohistochemistry techniques and bone wafer pit assay.Results The constructed recombinant plasmid contained the sequence of hOPG gene.After transfection with the plasmid,active OPG protein could be expressed in C2C12 cells.Conclusion The constructed eukaryotic expression bicistron plasmid vector pcDNA3.1-hOPG can express OPG in vitro,which is the basis for further study on the treatment of bone absorption caused by abnormal activity of osteoclasts.

Objective To investigate the influence of fixation of both lower limbs with negative pressure vacuum cushion and fixation of both ankles with self-made foam mat on setup errors in radiotherapy for rectal cancer.Methods A total of 12 patients with rectal cancer were enrolled in 2014 and randomly divided into group A (using negative pressure vacuum cushion) and group B (using self-made foam mat).An offline registration analysis was performed for the images of 108 times (A,B group of 54 times) of kilovoltage cone-beam CT (CBCT) before and after treatment.Grey scale translation error registration was used,and the results of registration were analyzed.The setup errors in x-axis (left-right direction),y-axis (cranial-caudal direction),and z-axis (anterior-posterior direction) were compared between the two groups.Results There was no significant difference in the absolute setup error in the y-axis between the two groups (2.13±0.64 mm vs.2.61±1.17 mm,P=0.399),while group A showed significantly lower absolute setup errors in the x-axis and z-axis than group B (x-axis:1.51±0.28 mm vs.2.70±1.05 mm,P=0.039;with an error rate of 7.41％ vs.42.59％;z-axis:1.10±0.29 mm vs.2.37±0.71 mm,P=0.002;with an error rate of 1.85％ vs.35.19％).Conclusions In the radiotherapy positioning for rectal cancer,fixation of both lower limbs with negative pressure vacuum cushion effectively avoids the translation and rotation of both lower limbs,reduces absolute setup errors,and has higher accuracy than fixation of both ankles with self-made foam mat.

This study is to investigate the effects of ubenimex on tumor cell invasion and apoptosis, dose relationship and mechanism. Immunofluorescence staining was performed to detect the expression of CD13 in HT-1080 cells. MTT assay was used to analyze the effect of ubenimex on cell proliferation. Annexin V-EGFP/PI was used to detect apoptotic cells by flow cytometry. Cell cycle was analyzed using flow cytometry. Ala-pNA was used as substrate to evaluate the effect of ubenimex on the aminopeptidase activity. Transwell assay was used to analyze the effect of ubenimex on cell invasion and migration ability. Western blotting was used to detect the expression level of CD13. MMP activity was analyzed using gelatin zymography. The results showed that ubenimex at high concentration inhibited the proliferation of HT-1080 cells (IC50: 3.8 mg x mL(-1)), and induced cell apoptosis. Cell cycle was blocked at G1 phase. Ubenimex at low concentration inhibited the aminopeptidase activity of HT-1080 cells (IC50: 8.3 microg x mL(-1)) and inhibited cell invasion, but it had no effects on the cell migration and proliferation. Ubenimex had no effects on CD13 expression and MMP activity. In conclusion, ubenimex at low concentration can inhibit the invasion ability of tumor cells by directly inhibiting the aminopeptidase activity; ubenimex at high concentration can inhibit the proliferation of tumor cells and induce cell apoptosis by a CD13-independent pathway.