Objective To explore the relationship between the protein expression of PCNA and biological behavior science in benignant pituita ry adenoma.Methods The protein expression of PCNA in 58 patien ts with pituitary adenoma were determined by ABC immunohistochemical method.Results The PCNA index was significantly higher in the patients with recurrent pituitary adenomas than in nonrecurrent ones(P<0.05).There was no significantly difference between bleeding and unbleeding group,cystic and noncystic group,large type and unlarge type group(P>0.05 respectively) .Conclusion The protein expressions of PCNA reflected the proli ferative activties of pituitary adenomas, and could be taken as one of the indic ators to evaluate recurrence and prognosis of the tumor.

Aged ; Apraxias ; physiopathology ; Gait Apraxia ; physiopathology ; Humans ; Parkinson Disease ; diagnosis ; physiopathology

Alzheimer Disease ; metabolism ; Animals ; Antioxidants ; metabolism ; Mice ; Polysaccharides ; pharmacology

Animals ; Curcumin ; pharmacology ; Diabetes Mellitus, Experimental ; drug therapy ; Rats

Animals ; Burns ; drug therapy ; metabolism ; physiopathology ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Female ; Male ; Mesentery ; blood supply ; Microvessels ; drug effects ; physiopathology ; Phytotherapy ; Rats ; Rats, Sprague-Dawley ; Superoxide Dismutase ; metabolism ; Trillium ; chemistry

Objective:To analyze neuropsychological characteristics of a Chinese patient with semantic dementia (SD)。Methods:A patient with SD was selected to finish 11 neuropsychological tests and MRI and XeCT。Results:(1)Selective impairment of semantic memory caused severe anomia, impaired spoken and written single-word comprehension, reduced generation of exemplars on category fluency tests and an impoverished fund of general knowledge; (2)relative sparing of other components of language output and comprehension; normal perceptual skill and non-verbal problem-solving abilities; relatively preserved episodic memory; (3)a reading disorder with pattern of surface dyslexia;(4) radiological investigations (MRI and XeCT) have shown severe left temporal neocortex atrophy.Conclusion:There are similar clinical and neuropsychological characteristics for SD between the Chinese patient and the sample of Western. Selective impairment of semantic memory may bring about by left temporal neocortex atrophy。

Objective To construct mouse granulocyte-macrophage colony stimulating factor (mGM-CSF) gene eukaryotic expressing plasmid pcDNA3-GM-CSF, to transfect the recombinant into erythroleukemia cell line FBL-3, and identify their biological activity.Methods GM-CSF gene eukaryotic expressing plasmid was constructed by subclone and recombinant was transfected into FBL-3 cells by electroporation. After screening by G418 and cloning by limiting dilution,we obtained positive cell clones(FBL-3-GM-CSF). PCR and RT-PCR were used to identify the integration and stable expression of GM-SF gene in FBL-3-GM-CSF cells. The biological activity was confirmed by the hematopoietic progenitor cell proliferative assay and hematopoietic progenitor cell colony formation assay. Results Mouse GM-CSF cDNA was amplified from the prokaryotic expressing plasmid PET-30a(+)-GM-CSF by PCR firstly and BamH Ⅰ and EcoRⅠrestriction sites were introduced. The inserted fragment was cut by BamH Ⅰ and EcoR Ⅰ digestion and ligated into pcDNA3 vector. The pcDNA3-GM-CSF eukaryotic expressing plasmid was constructed. The recombinant was cleared with appropriate endoneucleases and sequenced. The findings showed that the orientation of the insert was correct, while no rearrangement or mutation was found. PCR and RT-PCR assay showed that GM-CSF gene had integrated into FBL-3-GM-CSF cells and stably expressed. The hematopoietic progenitor cell proliferative assay and hematopoietic progenitor cell colony formation assay demonstrated that the cultured supernatant of FBL-3-GM-CSF cells of expressing GM-CSF should obviously stimulate proliferation of murine marrow mononuclear cells, and could stimulate hematopoietic progenitor cell colony formation. The number of colony formation was 54.67?4.83. The rate of colony formation was 0.547 %.Conclusions GM-CSF gene eukaryotic expressing plasmid is constructed successfully. A cell clone, which can express stably GM-CSF gene and possess biological activity,is obtained. Our studies have founded the base for the preparation of GM-CSF gene-modified vaccine of tumor cell and the study of feasibility of immune therapy of leukemia.

The G395R mutation of human sodium/iodide symporter gene was investigated by PCR-RFLP in 52 children with congenital hypothyroidism and 106 health children. The result suggested that G395R mutation may not be the main cause of congenital hypothyroidism in Qingdao.

Objective: To develop a grinding intrument for removing the remaining bonding material and polishing the dental enamel after orthodontic treatment. Methods: A dental griding instrument was made based on ultrasonic principle with the frequency of 25～45 kHz. 60 premolars extracted for orthodontic purpose were used in the experiment, before treatment the surface roughness was measured and recorded as Ra 1. Brackets were adhered to the premolars, and then debonded. The surface enamel of 30 teeth were polished by the ultrasonic grinding instrument (group of GI), that of another 30 by conventional technique(group of CT) , the roughness was measured and recorded as Ra 2. Results: In group GI Ra 1 and Ra 2 were 0.846 6?0.428 2 and 0.740 5?0.372 1(P0.05), respectively. After treatment Ra 2 in group GI was smaller than that in group CT(P

Objective:The aim of the present study was to examine the relationships between alexithymia, negative affect,and quality of life(QOL)of lung cancer patients receiving chemotherapy.Methods:51lung cancer patients receiving chemotherapy completed questionnaires of quality of life questionnaire for Chinese cancer patients receiving chemobiotherapy(QLQ-CCC),26-item Toronto alexithymia scale(TAS-26)and symptom checklist (SCL-90).Results:1)The patients had better score in QLQ(increased by 11%,P