Humans ; Multiple Myeloma ; blood supply ; therapy ; Neovascularization, Pathologic ; therapy ; Prognosis

Humans ; Maintenance Chemotherapy ; Multiple Myeloma ; therapy

BACKGROUND:Due to quantity and quality deficiencies, chondrocytes from microtia are difficult to act as seed cells to construct an ear cartilage scaffold with the normal human auricle size. OBJECTIVE: To test the hypothesis that auricular chondrocytes from microtia can promote chondrogenic differentiation and chondrogenesis of human adipose tissue-derived stem cells (ADSCs) at non-chondrogensis sitein vivo, which is the preparatory work for preparation of human tissue-engineered auricle cartilage scaffold. METHODS: Human ADSCs at passage 3 and auricular chondrocytes at passage 2 were mixed at a ratio of 7:3 and 5.0×1010/L mixed cells were suspended in 0.2 mL of 30% Pluronic F-127, and then the mixture was injected subcutaneously into Balb/c nude mice as experimental group. Auricular chondrocytes or ADSCs at the concentration of 5.0×1010/L were mixed with 0.2 mL of Pluronic F-127 and injected respectively as positive and negative control groups. 1.5×1010/L auricular chondrocytes were mixed and injected as low-concentration chondrocyte control group. All specimens were collected at the 8th week post-injection. Newborn tissues in nude mice were taken out for morphological examination, wet weight measurement, determination of glycosaminoglycans, histological and immunohistochemical staining. RESULTS AND CONCLUSION:The wet weight of specimens in the experimental group was over 80% of that in the positive control group, and the wet weight of specimens in the low-concentration chondrocyte control group was less than 30% of that in the positive control group. The average wet weight and glycosaminoglycan content were significantly higher in the experimental and positive control group than in the negative control and low-concentration chondrocyte control groups (P < 0.05). In all the groups except for the negative control group, mature cartilage lacunas could be observed by histological staining and collagen type Ⅱ could be detected for expression by immunohistochemistry to different extents. In the low concentration chondrocyte control group, cartilage lacunas were incompact and inhomogeneous, and the extracellular matrix was slightly stained. In the negative control group, mature cartilage lacunae and collagen type Ⅱ could not be detective. To conclude, auricular chondrocytes from microtia can promote chondrogenic differentiation and chondrogenesis of ADSCs at the non-chondrogenesis sitein vivo.

OBJECTIVE:To explore the ways of preventing errors in the process of drug treatment.METHODS:Related overseas literatures were consulted;causes of errors in the drug treatment were analyzed,which combined with prac-tice.RESULTS&CONCLUSIONS:Errors can be found effectively and reduced by establishing independent checking and error reporting system.

Objectives To study the correlation between social emotion and temperament in infants and toddlers. Me-thods The Brief Infant-Toddler Social and Emotional Assessment (BITSEA) and Toddler Temperament Questionnaire (TTQ) were used to evaluate the social emotional problems and temperament of 657 infants and toddlers 1 to 3 years old. Results The temperament dimensions were correlated with behavior and competence factors. The infants and toddlers in dififcult type group had more behavior problems or deifcits/delays in competence than those in easy type group (χ2=26.49, 10.48, P<0.01). Conclu-sions Infants and toddlers with negative temperament traits have more behavior problems and weaker social emotional compe-tences. The temperament should be considered in dealing with emotional problems.

Rectal cancer is a common malignant tumor in the gastrointestinal system.For the patients with resectable but high-risk rectal cancer,neoadjuvant and adjuvant chemoradiation plus surgery may improve local control.Compared to adjuvant chemoradiation,neoadjuvant chemoradiation showed the advantage of sphincter preservation.When the goal of preoperative chemoradiation was sphincter preservation,conventional radiotherapy was recommended.Through the analysis of the patients with 5-year survivals,the radiation had a similar impact on the survival as the chemotherapy alone for the patients with the intermediate risk factors.The impact on local control need to be further investigated before recommending chemotherapy alone for those subset patients.Advances in preoperative irradiation,postoperative irradiation and the comparison of preoperative irradiation with postoperative irradiation are also reviewed in this article.

Objective To investigate the significance of blood donation in preventing metabolic syndrome (MS)by analyzing the changes of the blood pressure and the related biochemical indexes before and after blood donation in the blood donors with high risk of MS.Methods The blood specimens of 102 blood donors,aged 45 -55 years old with the systolic blood pressure of 110 -140 mmHg and the diastolic blood pressure of 75 -90 mmHg,the body mass index> 24 kg/m2 ,were selected as the subjects.The blood pressure,heart rate,iron reserves,blood glucose,blood lipids,and the other indicators were determined before blood donation. All the above indicators were tested again on 42 d after blood donation.Results The blood pressure,iron reserves,blood glucose and LDL/HDL ratio after donation showed significant changes compared with before blood donation(P <0.05 ).Conclusion The changes of the MS related blood indexes exist before and after blood donation,blood donation has a certain significance for preven-ting MS in the blood donors with high risk of MS.

Usually individualized comprehensive treatments are applied to children with cerebral palsy. Among them, Kinesio taping is a simple treatment compared to orthosis, which allows greater rang of motion (ROM) and can more easily be accepted by patients, however, its effectiveness is controversial. This article introduced the mechanism that mainly includes relieving pain, improving joint mobility, increasing strength, relieving edema and increasing proprioception. The application of it in upper limbs, lower limbs and trunk of children with cerebral palsy was discussed. Finally, the advantages and limitations of Kinesio taping, suitable types of cerebral palsy and the therapeutic evaluation were analyzed.

Adolescent ; Adult ; Child ; Ear Auricle ; surgery ; Female ; Humans ; Male ; Postoperative Complications ; surgery ; Reconstructive Surgical Procedures ; Skin Transplantation ; Surgical Flaps ; Young Adult

Objective: To assess the relationship between pre-transplantation α-fetoprotein messenger (AFP) RNA expression in peripheral blood with tumor recurrence and survival rate in HCC patients. Methods: Thirty-one HCC patients receiving in situ liver transplantation from Feb. 2003 to Feb. 2004 were included in this study. Real-time quantitative RT-PCR was used to determine the expression level of AFP RNA in peripheral blood of all patients. All the cases were followed up for tumor recurrence and survival rate. The SPSS 12.0 software was used to analyze the relationship between AFP mRNA expression with tumor recurrence and survival rate. Results: Ten of the 31 patients were positive of AFP mRNA, with the quantity being 57 to 10 400 copies/μg RNA (median value: 360 copies/ μg RNA). Patients' Child-pugh grade, tumor size, tumor number, and tumor Edmood grade were not related to pre-transplantation AFP mRNA expression. Patients of pTNM III-IV stages had a significantly higher AFP mRNA expression than those of pTNM I-II stages (P=0.001); patients with vascular invasion had a significantly higher expression than those without (P=0.029). Patients who were negative of AFP mRNA before transplantation had a significantly higher survival rate than those positive of AFP mRNA. Conclusion: The results suggest that the pre-transplantation levels of AFP mRNA in peripheral blood may be a risk factor for recurrence and/or metastasis after transplantation in HCC patients; it may also be an indicator for extra-hepatic micrometastasis before transplantation and can be used for predicting prognosis.