15-deoxy-△12,14-prostaglandin J2(15d-PGJ2) is one of the natural ligands of the peroxisome proliferator-activated receptor γ.It plays an important protective role during cerebral ischemia.This article reviews the recent progress in research on the neuroprotective mechanism of 15d-PGJ2.

· AIM: To investigate the possibility of FLK-1 as a therapeutic agent for choroidal neovascularization (CNV) in mouse by FLK1 monoclonal antibody.· METHODS: CNV was induced in C57BL/6 mouse eyes by krypton laser photocoagulation. Preoperatively and on the 3rd, 6th and 9th day after photocoagulation, the animals were intraperitoneally injected with 500μg FLK1 monoclonal antibody. Choroidal fluorescein angiography and histopathological examination were used to assess the development of experimental CNV quantitatively 10 days after laser treatment.· RESULTS: Laser-induced CNV developed in all lesions on the 10th day after the operation. The development of CNV was significantly inhibited in experimental group, indicated by choroidal fluorescein angiography and histopathological examination (P＜0.001).· CONCLUSION: Our findings suggest that FLK-1 may be a promising agent for anti-angiogenesis therapy for CNV.

AIM: To investigate the expression of vascular endothelial growth factor receptor 2 (VEGFR-2, also known as FLK-1) in laser-induced choroidal neovascularization (CNV) in mouse.METHODS: CNV was induced in C57BL/6 mouse eyes by krypton laser photocoagulation. Choroidal fiuorescein angiography and histopathological examination were used to assess the development of experimental CNV. Cryostat sections from lesions on day 10 after laser treatment and normal eyes were prepared for Immunohistochemistry for FLK-1.RESULTS: Laser-induced CNV developed in all lesions on day 10. The expression of FLK-1 was detected in endothelial cells, retinal pigmented epithelium (RPE)-like cells and fibroblast-like cells in neovascular lesions. In normal adult mouse retinas, FLK-1 expression was mainly observed in RPE cells, inner nuclear and ganglion cell layers.CONCLUSION: Our findings demonstrated that expression of FLK-1 may play a role in the formation of laser-induced CNV in mice, which suggest that FLK-L may be a promising potential target for antiangiogenesis therapy for CNV.

Adult ; Aged ; Biomarkers, Tumor ; analysis ; Carcinoma, Non-Small-Cell Lung ; blood ; genetics ; pathology ; Cell Differentiation ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Keratin-19 ; genetics ; Leukocytes ; metabolism ; Lung Neoplasms ; blood ; genetics ; pathology ; Male ; Middle Aged ; Neoplasm Metastasis ; Neoplasm Staging ; RNA, Messenger ; biosynthesis ; genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Survival Analysis

Adult ; Dust ; analysis ; Female ; Humans ; Male ; Middle Aged ; Neural Networks (Computer) ; Occupational Exposure ; Silicosis ; diagnosis ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; methods

Objective To study the content of taxol in different parts of Taxus Yunnanensis and the effects of different growth time on taxol content. Method A HPLC method was developed for the detetmination of taxol content in bark, needle, twig, root from Taxus Yunnanensi, and the effects of different growth time. Results Taxol content in bark was the highest, that in root was the lowest and that in needle and twig were higher. Taxol content was higher with the growth time in needle and twig of Taxus Yunnanensi. Conclution It was significant to take taxol from needle and twig of Taxus Yunnanensis which is more than five years growth time.

Objective To control the quality of Herba Leonuri by studying the content of stachydrine hydrochloride in different parts of it. Method A HPLC method was developed for the content detetmination of stachydrine hydrochloride in flower, stem, leafage from Herba Leonuri. Spherisorb SCX colum was used with mobile phase of 20 mmol/L sodium dihydrogen phosphate (containing 0.04% triethylamine and 0.15% phosphoric acid). The colum temperature was 25 ℃, the detective wavelength was 192 nm. Results Stachydrine hydrochloride content was the highest in leafage and the lowest in stem. Conclusion To ensure the quality of Herba Leonuri, it is significant to choose medical material with more leafage.

Objective To evaluate the effects of different intensities of treadmill running on changes in tibial bone composition in a rat model.Methods A total of 24 female Wistar rats were randomly assigned into 4 even groups (n =6) of free movement (FM),low-intensity running (LR),medium-intensity running (MR),and high-intensity running (HR).Rats in LR,MR,and HR groups underwent treadmill running with respective intensities (15 m/min and a 0° gradient,20 m/min and a 5° gradient,25 m/min and a 10° gradient) for 8 weeks (1 h/d and 5 d/week).FM group were taken as controls.The cortical and trabecular bone at the left tibial metaphysis was harvested after 8 weeks in each group for Raman spectroscopy to analyze mineral-matrix ratio,carbonate-phosphate ratio and crystallinity.Results In MR group,the mean mineral-matrix ratio was 4.883 ± 0.128 for cortical bone and 4.216 ±0.213 for trabecular bone,both significantly higher than those in FM group (4.113 ±0.132 and 3.773 ±0.122) (P ＜ 0.05).The mean mineral-matrix ratio was 3.222 ± 0.329 for trabecular bone in HR group,significantly lower than that in FM group (P ＜ 0.05).In MR group,the mean carbonate-phosphate ratio was 0.166 ± 0.013 for trabecular bone,significantly lower than that in FM group (0.177 ± 0.011) (P ＜ 0.05).In HR group,the mean carbonate-phosphate ratio was 0.195 ± 0.012 for cortical bone and 0.187 ± 0.010 for trabecular bone,both significantly higher than those in FM group (0.183 ± 0.014 and 0.177 ± 0.011) (P ＜ 0.05).In HR group,the mean crystallinity was 0.0521 ± 0.0012 for cortical bone and 0.0522 ± 0.0017 for trabecular bone,both significantly higher than those in the other 3 groups (P ＜ 0.05).Conclusions Running with medium intensity may increase bone mineralization and turnover,thus enhancing bone quality.However,running with high intensity may decrease bone mineralization and thus bone quality.

For comprehensive ability's cultivation of clinical graduate students,this article presents new and feasible methods in placing emphasis on cultivating teaching ability,in order to improve clinical graduate student's teaching ability.It also provides feasible experience and lessons for many other medical schools on graduate cultivating and management.

Objective By studying the acute brucellosis patients' blood culture and the bacteria strains,we tried to confirm the bacteria strains,and to further explore the symptoms and the results of the treatment.Methods Totally 122 acute brucellosis patients were selected,who were in hospital in Heilongjiang Provincial Nongken General Hospital from 2012 to 2013.The patients aged 6-73 years old,whose average age was 36.5(including 83 males and 39 females).All of the subjects were detected in the aspects of blood culture and brucellosis serology,all of whom were treated with aminoglycoside,4-quinolones and cephalosporins.Results All the subjects were Brucella melitensis biovar,of which 104 were Brucella melitensis biovar 3,18 were Brucella melitensis biovar 1.All of them had positive results in rose bengal plate agglutination test (RBPT).In serum tube agglutination test (SAT),the valence of Brucella melitensis biovar 1 and 3 were uniformly distributed,Brucella melitensis biovar 1 was distributed mainly in 1∶800 valence zone,Brucella melitensis biovar 3 was distributed mainly in 1∶400 valence zone.The distribution trends in Coombs test and complement fixation test (CFT) were similar.In Coombs they were distributed mainly in 1∶800 valence zone,and in CFT were distributed mainly in 1∶40 zone.All of the subjects had typical symptoms of brucellosis,and had various kinds of complications (spondylitis,toxipathic hepatitis,et al).Totally 112 of them were recovered,and 10 were improved.Conclusion The human brucellosis in Heilongjiang Province is caused by Brucella melitensis biovar 1 and 3,with severe symptoms and various kinds of complications,earlier diagnose and treatment will have a better effect.