Clozapine ; adverse effects ; Haloperidol ; adverse effects ; Humans ; Infant, Newborn ; Male ; Neonatal Abstinence Syndrome ; etiology

OBJECTIVE:To study anti-arrhythmic effects of Lianshen lyuping dripping pills in rats,mice,guinea pigs. METH-ODS:48 SD rats were randomized into a normal control (isometric normal saline) group,a model (isometric normal saline) group,a propafenone (positive control,270 mg/kg) group and Lianshen lyuping dripping pills high-dose,medium-dose and low-dose(210,140,70 mg/kg)groups. The rats’tails were given the drugs iv at a time,10 min later,aconitine(10 mg/L,3 μg/min) iv. The doses of aconitine were recorded when ventricular premature beat(VP),ventricular tachycardia(VT)and ventricular fibril-lation(VF)developed in ECG. 48 guinea pigs were grouped and given drugs as above and 10 min later,were given uabain iv(1 mg/L,1 μg/min). The doses of uabain were recorded when VP,VT and VF developed in ECG. 120 KM mice were grouped and given drugs ip at a dose as above at a time,30 min later,were placed in the beaker containing 4 ml chloroform. When respiratory arrest occurred,ventricular fibrillation rate of mice were calculate.120 Wistar rats were grouped and given drugs at a dose as above,following typical arrhythmia reaction,given 0.8% BaCl2 (0.25 ml/kg) iv and sublingually at a time;48 Wistar rats were grouped and given drugs at a dose as above and,following typical arrhythmia reaction, given 5%CaCl2(0.28 ml/kg)iv and sublin-gually at a time. Recovery of sinus arrhythmia rate of rats were determined.48 SD rats were grouped and given drugs at a dose as above,after the administration ig once daily for 7 consecutive days,the rat models of myocardial ischemia reperfusion were estab-lished,and then VT,VF incidence rate and death were recorded. RESULTS:Compared to the normal control group,those in the model group were all abnormal in all indexes. Compared to the model group,those in Lianshen lyuping dripping pills high-dose, medium-dose and low-dose groups used more aconitine and uabain at the time of the occurrence of VP,VT and VF,had a lower occurrence rate of VF in mice and higher recovery of sinus arrhythmia rate in rats . The rats in Lianshen lyuping dripping pills high-dose and medium-dose groups which had myocardial ischemia reperfusion demonstrated a lower of VT,VF incidence rate and death rate. There were statistically significances (P<0.01 or P<0.05).CONCLUSIONS:Lianshen lyuping dripping pills have an anti-arrhythmic effects in animals to some degree.

Objective To investigate the protective effects of ischemic preconditioning on myocardial ischemia/reperfusion injury in diabetic rats.Methods Twenty-eight healthy male rats were injected streptozotocin at dose of 45 mg/kg by tail and be fed with normal diet for 4 weeks,then rats were randomly divided into iscbemia/reperfusion (I/R) group and ischemic preconditioning (IP) group.ST segment of electrocardiograph changes and arrhythmias of all rats were recorded before ischemia and 0,15,30 minuets after ischemia and 0.5,2 h after reperfusion.TTC staining was performed to determine myocardial infarct size.TUNEL assay was used to assesse cardiomyocyte apoptosis.The expression of antiapoptotic gene (Bcl-2) and proapoptotic (Bax) was determined by immunohistochemistry.Results Compared with I/R group,ST segment elevation of patients in IP group decrease from (0.675 ±0.150) mV to (0.489 ±0.161) mV at 30 min after ischemia(P ＜0.05).Meanwhile the onset of ventricular premature contraction(VPC) in IP group was (18.21 ± 5.36) min,later than that of control group ((6.47 ± 4.28) min,t =5.241,P =0.000).The duration of VPC was (6.07 ± 4.33) min,shorter than that of I/R group ((16.71 ± 5.48) min,t =4.924,P ＜ 0.01)).The incidence of ventricular tachycardia (VT) and ventricular fibrillation (VF) of lP group remarkably decreased compared with I/R group (VT:57.14％ (8/14) vs.14.29％ (2/14),x2 =5.600,P =0.018 ; VF:50.00％ (7/ 14) vs.14.29％ (2/14),x2 =4.094,P=0.043).The myocardial infarct size in IP group was (12.50 ± 9.45) ％,smaller than that of I/R group ((37.50 ± 11.40)％,t =3.211,P =0.006).Cardiomyocyte apoptotic index (AI) was attenuated in IP group than that of I/R group((24.31 ± 3.12)％ vs.(19.01 ± 4.32)％,t =3.227,P =0.006),which was correlate with increased the ratio of Bcl-2/Bax((0.103 ±0.045) vs.(0.221 ±0.101),t =2.670,P =0.015).Conclusion IP treatment for diabetic rats shows a protect effect on myocardial I/R injury through attenuating myocardial apoptosis,and increasing the ratio of Bcl-2/Bax.

Cholestasis ; etiology ; physiopathology ; therapy ; Female ; Humans ; Liver Diseases ; drug therapy ; etiology ; physiopathology ; Male ; Neonatology ; Parenteral Nutrition, Total ; adverse effects ; S-Adenosylmethionine ; therapeutic use ; Ursodeoxycholic Acid ; therapeutic use

The trophoblast is fetal-derived and in direct contact with maternal tissues. The expression of the various types of MHC antigen in trophoblast cells is very important for successful pregnancy. Among nonclassical MHC class Ⅰ antigens, HLA-G is expressed specifically on cytotrophoblast cells and its expression protects the fetal from maternal immune attack. The expression of the distinct subtypes of classical MHC class Ⅰ antigen is different in different subtypes of trophoblast cells. The expression of MHC class Ⅱ antigen is inhibited in trophoblast during pregnancy. It is suggested that C Ⅱ TA plays an important role in regulation of MHC class Ⅱ gene expression.

The paper first discusses the concept of hospital performance management systems and their relationship with the business systems and then dwells on the framework of the performance management systems, including extended business systems, database systems of shared business, subject driven systems and commercial intelligence systems. It also gives an account of the results of actual application of the Hospital Operational Performance Management Systems developed by the hospital the authors work with.

Objective To observe the expression of fractalkine (FKN or CX3CL1) in serum and lung tissue in early phases after paraquat (PQ) poisoning in rats,and to analyze the effect of FKN on acute lung injury induced by PQ.Methods A total of 66 SD rats were randomly (random number) divided into two groups,namely PQ group (n =36) and control group (n =30).Through intra-peritoneal route,PQ (22 mg/kg) was administered to PQ group,and an equal volume of normal saline to control group instead.Rats were separately sacrificed at 6 h,12 h,24 h,72 h and 120 h after poisoning.Lung coefficient was determined.The levels of FKN in serum and lung tissue homogenate were detected by ELISA.Lung pathological changes were observed by HE staining.FKN changes were investigated by immunohistochemistry staining.Data was analyzed by SPSS 19.0.Results From 6 h to 120 h after poisoning,parameter determined in PQ group had great changes,compared with the control group.At 6 h,12 h,24 h,72 h and 120 h,lung coefficients in PQ group were 5.03 ±0.07,5.17 ±0.10,5.46 ±0.10,5.68 ±0.15 and 5.83 ±0.11,respectively,which were significantly higher than those (4.49 ± 0.20,4.28 ± 0.13,4.45 ± 0.17,4.31 ±0.19 and 4.31 ±0.16) in control group (P＜0.01).Levels of FKN (pg/mL) in serum were 140.9 ± 15.8,157.9 ± 17.6,188.8 ± 24.8,224.4 ± 18.1 and 229.9 ± 10.0,respectively,significantly higher than those (121.7 ± 12.8,121.6 ± 12.1,118.3 ± 14.0,122.8 ± 12.4 and 120.5 ± 8.8) in control group (6 h P ＜0.05,others P ＜0.01).Levels of FKN (pg/mL) in lung tissue homogenate were 4 222.0 ± 641.1,5 021.0 ± 514.5,5 911.6 ± 478.1,7 092.2 ± 652.9 and 7 639.3 ± 666.6,respectively,significantly higher than those (2 860.2 ± 477.3,3 068.9 ± 446.0,3 168.7 ± 728.5,3 178.0 ±488.2 and 3 147.3 ±426.6) in control group (P ＜0.01).In PQ group,pathological changes manifested themselves in inflammatory cell infiltration,congestion,edema,structural damage,etc.The lung injury aggravated gradually from 6 h to 120 h.In control group,there was no significant change.FKN expressed mainly in bronchial cells,alveolar epithelial cells and pulmonary artery endothelial cells.Where there was higher expression of FKN,there were more inflammatory cells infiltrated.The level of FKN in lung tissue homogenate was positively correlated with lung coefficient (r =0.937).The level of FKN in serum was positively related to that in lung tissue homogenate (r =0.968).Conclusion There is correlation between FKN and acute lung injury induced by PQ in rats.

Adult ; Humans ; Male ; Nasal Cavity ; pathology ; Orbit ; pathology ; Paranasal Sinuses ; pathology ; Skull Neoplasms ; pathology ; surgery

BACKGROUND: Many scholars attempt to xenotransplantation because of shortage of human donor hepatocytes. In the field of hepatocyte xenotransplantation, two different mammalian species, i.e., pig-to-rat, and pig-to-rodent, are much reported. Hepatocyte transplantation between two different classes has been rarely reported. OBJECTIVE: To investigate the mechanisms of cell-mediated immune response in hepatocytes xenotransplantation between tilapia and rat. METHODS: The tilapia (donor) hepatocytes were isolated by collagenase cold digestion and adjusted to 2×107 /mL using physiological saline. Thirty-six SD (recipient) rats were randomly divided into transplantation and control groups (n = 18). Rats from the control group received injection of physiological saline into the spleen, and those from the transplantation group received injection of tilapia hepatocytes. At each time point (4 hours, 8 hours, 24 hours, 3 days, and 5 days) after transplantation, two rats were respectively sacrificed for histological changes examination using hematoxylin-eosin staining and detection of CD4+/CD8+ lymphocytes surrounding the grafts using immunohistochemical SABC method. RESULTS AND CONCLUSION: The transplanted hepatocytes were rejected a few hours after transplantation. Surviving hepatocytes in spleens were hardly seen 8 hours after transplantation. The aggregation of CD4+CD8+ lymphocytes was detected surrounding the grafts 4 hours after transplantation. These findings suggest that tilapia hepatocytes transplantation into rat spleen induces rejection within a few hours, cell-mediated immune response is involved in the rejection of hepatocytes xenotransplantation, and natural killer cells may play an important role in this kind of rejection.

Objective To determine the efficacy of coronary artery bypass grafting (CABG) in children with coronary obstructive disease subsequent to Kawasaki disease. Methods Between Feb 2005 and Sep 2009, 6 children with ischemic heart disease due to Kawasaki disease comprised the study group. The age of patients at operation was ranged from 6 to 12 years with a mean of ( 8.0 ± 2.3 ) years. The preoperative EF was ranged from 0.33 to 0.71 with a mean of 0.57 ± 0.15. There was one case with moderate mitral valve regurgitation. Results All patients survived the procedures. The mean number of bypass graft was 2.0 ±0.6,with 4 internal thoracic arteries and 7 radial arteries. Mitral valve repair was employed in 1 case. The cardisc function ( NYHA and EF) was significantly improved postoperatively. Postoperative CTA showed no graft disease in all cases. Conclusion CABG using the arterial grafts can provide attractive mid-term results in patients with obstructive coronary arteries associated with Kawasaki disease.