Objective To analyze the correlation of discomfort experience and factual recollection (FR) in patients of intensive care unit (ICU). Methods Fifty-five ICU patients and 63 ward patients par-ticipated in the study. Standardized questionnaires were used to evaluate all patients" FR and the results underwent analysis. Results The prevalence of recollection of any type of discomfort in the ICU patients was 64%. The presence of an endotracheal tube, pain and hallucinations were identified as sources of dis-comfort.The median score for FR in the ICU patients was 7.25. The median score for FR in the ward pa-tients was 13.50. The difference between them was highly significant (t =3.34, P < 0.01 ). Logistic analysis revealed that discomfort, especially discomfort caused by the presence of an endotracheal tube, pain and hallucinations, was positively related to FR, 0R95%CI (1.2～2.1), P < 0.01. Conclusions The presence of an endotracheal tube, pain and hallucinations were most frequently reported as sources of discomfort in ICU patients and their memory was often impaired. FR and recollection of discomfort appear to be related significantly in ICU patients.

ObjectiveTo study the expression of adhesion mol ecules (CD 54 、CD 62p ) on diabetic neuropathy in the STZ induced rats and the effect of cilostazol treatment.MethodsSD rats were divided into four groups: normal control, diabetes control, insulin and cilostaz ol treated group. Sciatic nerve conductive velocity, the level of CD 54 /CD 62p on the surface of mononuclear cell/platelet were examined, and ultrast ructure of sciatic nerve was observed.ResultsCilostazol incr eased sciatic nerve conductive velocity significantly [DM=(20.3?2.2) m/s vs ci lostazol=(28.9?7.9)m/s,(P

Peripheral artery disease is a cardiovascular risk factor. Exercise training may benefit patients with peripheral artery disease from preserving or improving functional capacity and reducing cardiovascular events. Accordingly, this article reviewed the effects of exercise training on peripheral artery disease, and the possible mechanisms

Objective To compare the application of three diagnostic criteria for metabolic syndrome(MS) among the elderly in community. Methods 773 elderly people in the community were diagnosed for MS by the Chinese Medical Association Diabetes Society (CDS),International Diabetes Federation (IDF) and revised National Cholesterol Education Prongram-Adult Treatment Panel(ATP Ⅲ* ) criteria,respectively.The diagnosis accordance rates were compared among the different diagnostic criteria. Results The MS prevalence rates in the elderly were 32.5％ (251/773),53.4％ (413/773) and 47.2％ (365/773) according to CDS,IDF and ATP Ⅲ* criteria,respectively and there were significant difference among the criteria (Q=173.10,P＜0.01 ).The MS prevalence was higher in women than in men especially by IDF [57.3％ (284/487)vs.45.1％ (129/286),x2=12.64,P＜0.01]and ATPⅢ* criteria [53.0％(258/487)vs. 37.4％ (107/286),x2=17.52,P＜0.01].The coincidences in the diagnosis of MS were 88.6％ (Kappa=0.773,P＜0.01)using IDF and ATPⅢ * definitions,75.7％ using CDS and ATPⅢ *,and 73.7％ using CDS and IDF.Conclusions The agreement in the diagnosis of MS using IDF and ATPⅢ* definitions is higher than using CDS and IDF or ATPⅢ * definitions.

Objective:To study the clinical application of laparoscopic combined with multi-video debridement in treatment of complicated infectious pancreatic necrosis (CIPN).Methods:The clinical data of 34 patients with CIPN who were treated at the Department of General Surgery, Xuanwu Hospital, Capital Medical University from August 2017 to December 2019 were retrospectively studied. Based on the different video methods used, these patients were divided into 3 groups: the laparoscopic combined with intraoperative ultrasound group, the laparoscopic combined with choledochoscopy group and the laparoscopic group. The number of operations, operation time, blood loss, postoperative complication rates, mortality rates and total length of hospital stay were compared.Results:There were 13 patients in the laparoscopic combined with intraoperative ultrasound group, with age of (56.4±13.4) years. There were 7 patients in the laparoscopic combined with choledochoscopy group, with age of (48.0±8.4) years. There were 14 patients in the laparoscopic group with age of (51.4±15.6) years. The number of operations of the laparoscopic combined with intraoperative ultrasound group, the laparoscopic combined with choledochoscopy group and the laparoscopic group were (2.2±1.1), (1.6±0.8), (2.9±1.4), respectively. The number of operations of the laparoscopic combined with choledochoscopy group were significantly less than that of the laparoscopic group ( P<0.05), but there were no significant differences among the other groups ( P>0.05). The operation time of the laparoscopic combined with intraoperative ultrasound group, the laparoscopic combined with choledochoscopy group and the laparoscopic group were (70.5±22.9) min, (65.7±19.9) min, (51.5±15.4) min, respectively. The operation time of the laparoscopic combined with intraoperative ultrasound group was significantly longer than that of the laparoscopic group ( P<0.05), but there were no significant differences among the other groups ( P>0.05). There were no differences in blood loss, postoperative complication rate, mortality rates and total lengths of hospital stay among the three groups ( P>0.05). Conclusion:Laparoscopic combined with multi-video debridement after making full use of the advantages of each of the video methods, can be used to improve treatment outcomes of patients with CIPN.

BACKGROUND: It is discovered by administrating different emetics to vomiting animals, like cats, that there are a large amount of Fos positive neuronal expressions in the arc region from nucleus of solitary tract, lateral tegmentum to ventrolateral area. And it has been viewed that the arc region from area postrema, nucleus of solitary tract to ventrolateral reticular structure is the main emetic region. Whether do the non-vomiting animals reflect in response or not after emetic injection?OBJECTIVE: To observe the distribution of Fos positive neurons in relevant emetic regions of brain and spinal cord in rats after abdominal injection of emetic, cisplatin.DESIGN: Randomized controlled experiment based on animals.SETTING: Neural Physiological Research Room of Life Science College in Hebei Normal University and Physiological Room of Basic Medicine Institute in Hebei Medical University.MATERIALS: The experiment was performed in Neural Physiological Research Room of Life Science College in Hebei Normal University and Physiological Room of Basic Medicine Institute in Hebei Medical University from March to August 2003. Twelve SD male rats were employed, body weighted varied from 220 to 250 g, of clean-grade. They were randomized into experimental group of 6 rats and the control of 6 rats.INTERVENTIONS: In experimental group, the emetic, cisplatin, was injected abdominally 10 mg/kg. In the control, the physiological saline of same dose was injected. Afterwards, the activity changes in rats were observed at room temperature, quiet and light-avoided environment. Six hours later, the brain tissue was collected for frozen continuous sectioning. Immunohistochemical staining method was used to observe the distribution of Fos positive neurons in brainstem and forebrain nuclei and to count positive cell.MAIN OUTCOME MEASURES: ① Behavior observation in rats after emetic injection. ② Counts of Fos positive cell in relevant regions of brain in rats.RESULTS: Twelve rats all entered result analysis. ① In 20 minutes after injection, the rats in both groups were in tranquilizing state, lying prone with body curled, almost without any movement. In 60 minutes after injection, the rats in the control were recovered to normal, free of eating or drinking. The rats in experimental group were in prone-lying state with body curled. They rose up or shook the heads occasionally, and they breathed fast and uneven.In 2 hours after injection, in experimental group, the rats were still in abdominal prone tightly in the cage, with heads lowed and irregular shaking of noses. In 5 hours, the rats in experimental group began standing up and moving, with normal breathing, but they still did not eat or drink. ② Fos positive neurons in solitary tract, area postrema and lateral parabrachial nucleus and paraventricular nucleus, supraoptic nucleus and arc nucleus in hypothalamus (64.3 ±9.6, 83.4 ±15.0, 148.8 ±19.9, 80. 2 ± 11.8, 20.7 ±3.8, 86. 6 ± 10.8) were remarkably higher than those in the control(56. 2 ±6.3,73.5±9.9,136.9±17.8,66. 1±10.3,17.3±3.4,78.8±10.5).CONCLUSION: Emetics induce discomforts in internal organs of rats, due to which, there probably exist emetic regions similar to vomiting animals in central neural system. But it is probably lack of vomiting-related adjusting mechanism. Emetics irritate the increase of Fos positive neurons in relevant regions in the brain of rat, which suggests that there exist relevant neural chemical pathways similar to nausea in the brain of non-vomiting rats.

OBJECTIVE:To study inhibitory effects of propofol on PC12 cells injury induced by glutamic acid via mitogen-acti-vated protein kinase/extra-cellular regulated kinase (MAPK/ERK) signal pathway. METHODS:PC12 cells were randomized into normal control group,model group(10 mmol/L glutamic acid),propofol low-concentrations,medium-concentrations and high-con-centrations groups(12.5,25,50 μmol/L+10 mmol/L glutamic acid). The optical density of cells,cell apoptosis,the phosphoryla-tion of ERK1/2 and the expression of c-fos,Bax,Bcl-2 were detected after treated with relevant medicine for 48 h. RESULTS:Compared with normal control group,optical density of cells,the phosphorylation of ERK1/2 and Bcl-2 decreased in model group (P<0.01);apoptotic rate,the expression of c-fos and Bax increased (P<0.01). Compared with model group,optical density of cells,the expression of Bcl-2 and the phosphorylation of ERK1/2 increased in propofol group (P<0.01);apoptosis rate,the ex-pression of c-fos and Bax decreased (P<0.05 or P<0.01). There were statistical significant between the different concentrations (P<0.01). CONCLUSIONS:Propofol can inhibit the apoptosis of PC12 cells induced by glutamic acid,which is associated with the up-regulation of ERK1/2 phosphorylation.

ive] To observe the effects of Shengjiang Capsule (SC) on gastric evacuation and to study its mechanism. [Methods] Eight-week-old SD rats were randomly allocated to five groups. Group A served as normal control, and Group B were treated with high dosage of SC, Group C with low dosage of SC, Group D with Zhike Baizhu Decotion and Group E with cisapride. After ten days of treatment, gas tric evacuation of rats was detected by isotope tracing method, erythrocyte-acetycholinesterase activity by micro-hydroxylamine method and plasma motilin (Mot) and somatostatin (SS) levels by radioimmunoas-say. [Results] Thirty-minute gastric evacuation rate was (51.44?.38)%, (40.82 + 7.24)% and (40.22?.16)% in Group B, Group C and Group D respectively, which was higher than that in Group A [ (33.18?.32)%]. Erythrocyte-acetycholinesterase activity, Mot and SS levels were (0.856?.128) umol/h, (124.26?5.94) ng/L and (39.42?.96) ng/L, and (0.726?.164 ) umol/h, (119.86 ?9.38) ng/L and (38.33 ?.64) ng/L in Group B and Group C respectively, which were higher than those in Group A [ (0.576 ?.150)/umol/h, (91.28 + 26.84) ng/L and (28.22 ?7.68)ng/L]. [Conclusion] SC can promote gastric evacuation, and its mechanism may be re lated to the increase of the cholinergic nerve function and plasma Mot and SS levels.

Objective: To establish the identification method of Gegen Qinlian Preparation(Radix Puerariae, Radix Scutellariae, Rhizoma Coptidis, and Radix Glycyrrhizae) by three dimensional HPLC. Methods: Puerain, baicalin and berberine were dissolved with 70% methanol as a reference solution. Gegen Qinlian preparation was analysed by three dimensional HPLC, methanol water (27∶73 containing 0.7‰ sodium lauryl sulfate and 0.7‰ triethylamine) as a mobile phase was used by linear gradient elution. Conclusion: The scientific and well defined identification of three dimensional HPLC can be showed by Gegen Qinlian Preparation research and this method is accurate and reliable.

Objective:To study the effects of phentolamine and dopamine combined with cardiopulmonary resuscitation on the patients with cardiac arrest.Methods:60 patients with cardiac arrest who were treated from February 2015 to April 2016 in our hospital were selected and divided into the control group and the observation group according to different treatment methods.The control group was treated with routine treatment.while the observation group was treated with phentolamine and dopamine based on the control group.The changes ofcTnI,CK,CK-MB levels,MDA,SOD concentration and hemodynamics were observed before and after treatment.Results:After treatment,the total mortality was 20％ in the observation group,which was significantly lower than that of the control group (33.33％,P＜0.05).The levels of cTnI in the observation group were significantly lower than those of the control group at 2 h and 24 h after the cardiopulmonary resuscitation(P＜0.05).After the cardiopulmonary resuscitation,the serum levels of CK and CK-MB in the observation group were significantly lower than those in the control group (P＜0.05).The concentration of MDA and SOD in the observation group were significantly higher compared with the control group [(1.86± 1.65) μg/L vs(3.81± 1.24) μg/L、(6.58± 0.95)μg/L vs(3.74± 0.56) μg/L](P＜0.05).After the cardiopulmonary resuscitation,the MAP value of observation group was significantly higher than that of the control group (P＜0.05).The CO value and CI value of observation group were significantly increased from the 6th day to the control group (P＜0.05),and the PCWP values of observation group were significantly lower than those of the control group (P＜0.05).Conclusion:Phentolamine combined with dopamine could effectively improve the cardiac hemodynamics and prognosis of patients with cardiac arrest,reduce the myocardial injury and enhance the survival with high safety.