Objective:To investigate the effects of U0126 of different doses on the ability of low frequency magnetic stimulation promoting astrocyte migration and to select suitable dose of U0126.Method:Twenty-four adult healthy SD rats were selected to receive Injection of 0.5ml of 1% ethidium bromide(EB) in PBS into the dorsal spinal cord funiculus on the left side at T10-11 level to make located spinal cord injury models and randomly divided into four groups.The four groups were exposed to magnetic stimulation(1Hz,1.52T.30pulses)at the following dose respectively:Omg/kg U0126(control group).0.1mg/kg U0126(low-dose group), 0.2mg/kg U0126(middle-dose group),0.4mg/kg U0126(high-dose group).On the day 14 after stimulation,the rats were sacrificed and the expressions of glial fibfillary acidic protein(GFAP),microtubule associated protein-2(MAP-2),extracellular signal-regulated kinase1/2(ERg1/2)and the volume of holes were detected with immunohistochemistry.Quantitative analysis 0f the expressions of GFAP,MAP-2 and ERK1/2 were performed with the image analysis system.Result:With the increase of U0126 dose,the volume of hole increased on day 14(p<0.05).In the lesion area,the expressions of GFAP and ERK1/2 could be found,while MAP-2 could not.Significant differences were revealed in the expressions of GFAP、ERK1/2 among the four groups,it Was significantly lower in U0126 groups than that in control greup(P<0.05).while the middle-dose group had similar effect with the high-dose group(P>0.05).Conclusion:U0126 of different doses all could resupinate astrocyte migrations which were coused by low frequency magnetic stimulation,and 0.2mg/kg was the suitable dose.

Objective To investigate the effect of magnetic stimulation on astrocyte migration and its mech-anism. Methods Twenty-four adult, healthy Spragne-Dawley rats were injected with 0.5 μl of 1% ethidium bro-mide (EB) in the left side of the dorsal spinal cord funiculus at the T_(10-11) level to make a local spinal cord injury mod-el. They were then randomly divided into four groups and exposed to 30 pulses of magnetic stimulation at 1 Hz and the following intensities: O T (Group A);1.9x40% T (Group B); 1.9x80% T (Group C); 1.9x100% T (Group D). On the 14th day after stimulation, the rats were sacrificed and the expression of glial fibrillary acidic protein (GFAP), microtubule associated protein-2 (MAP-2) and extracellular signal-regulated kinase 1/2 (ERK 1/2) were detected, and the volume of holes in the injured area of the spinal cord was measured. Quantitative analysis of the GFAP, MAP-2 and ERK1/2 expression was performed using immunohistochemistry and an image anal-ysis system. Results The volume of holes in the injured area of the spinal cord decreased with increasing stimula tion intensity. In the reduced area of the holes, the expression of GFAP and ERK 1/2 could be seen, but not MAP-2. Significant differences were revealed in the expression of GFAP and ERK 1/2 among the four groups, but it was always significantly higher in the magnetic stimulation groups than in the controls. Conclusions After magnetic stimulation, astrocytes migrate into the injured spinal cord's holes. Astroeyte migration increases with increased mag-netic stimulation intensity, which may be associated with high expression of ERK 1/2.

Objective To find out about the current health situation of the elderly in the Beijing area and their health care needs so as to provide basis for the formulation of health care policies for the elderly by the government and relevant departments and for the development of health care programs for the elderly in accordance with their needs. Methods A sample survey, covering such topics as the health situation, medical treatment, nursing, rehabilitation and health care needs of the elderly and community health service, was made of 2 487 elderly people aged 60 and above in different parts of Beijing, including the city proper, the part linking city and country, and the suburbs. An analysis was then made of the difference in the health situation and health care needs of the elderly between various parts and populations. Results Chronic diseases constituted the major health problem for the elderly, with hypertension, coronary heart disease, osteoarthrosis, and cerebrovascular disease ranking in the first four places and the incidence of diabetes increasing rapidly. About 10 percent of the elderly, who suffered from somatic dysfunction, had reduced daily life skills and needed assistance from others. An increased number of elderly people also meant a greater demand for medical and nursing services. Community health service, the advantages of which bacame increasingly manifest, was thus popular with the elderly. Financial difficulty was the chief factor affecting the elderly in their seeking medical service. Conclusion The elderly is a weak population in health. With the aging of the general population, the health care needs of the elderly are on the rise. As health is the major factor affecting the life quality of the elderly, enhanced efforts in the prevention and treatment of chronic diseases ought to be taken as the central task in the health care of the elderly. They are also the key to improving the life quality of the elderly.

Objective To investigate the relationship between serum uric acid (SUA) and metabolism syndrome (MS) in community-based elderly population in Beijing.Methods A community-based and cross-sectional survey on 1821 elderly person was performed in Beijing in 2000.Questionnaire survey,physical examination and blood biochemical tests were conducted.Relationship of MS and its components with SUA was analyzed.Results Among 1821 subjects,there were 365 cases with MS (20.0％) and 1456 cases without MS (80.0％).SUA level was higher in MS group than in non-MS group [(5.20±2.27) μmol/L vs.(4.50±1.44) μmol/L,t=-7.34,P=0.000].All subjects were divided into 4 groups according SUA level:goup 1 (≤3.5μmol/L,n=443),group 2 (3.6 μmol/L-4.4 μmol/L,n=465),group 3 (4.5 μmol/L-5.4 μmol/L,n=446) and group 4 (≥5.5 μmol/L,n=467).SUA level was increased along with the increased BMI,TG,TC,LDL-C levels,and decreased along with the decreased HDL-C level,which had significant differences (F=43.07,9.32,22.36,8.19,9.39,all P＜0.001).There were significant distribution differences in MS components numbers in the 4 groups (x2=73.62,P=0.000).After adjusting for age,sex,residential,education level,marriage,smoking,drinking and exercise habits,logistic regression analysis showed that MS was related with SUA level,and the risk of MS was increased along with the increased SUA level.Conclusions MS is correlated with SUA level in elderly people.SUA level should be observed and controlled effectively in the elderly.

This study is to determine the preventive effect and mechanism of targeting IL-17A on pulmonary inflammation and fibrosis after acute lung injury. Mice were treated with anti-IL-17A antibody on the day 7 and sacrificed on the day 14 after bleomycin lung injury. The pulmonary inflammatory status and the deposition of collagen were measured by HE and Sirius stains staining. The contents of hydroxyproline and collagen were measured by using commercial kits. The survival rate of mice was calculated by Kaplan-Meier methods. The inflammatory cytokines in bronchoalveolar lavage fluid were measured by ELISA and the expressions of inflammation-related molecules were detected by Western blotting assay. Targeting of IL-17A could prevent the development of lung inflammation, decrease collagen deposition and the contents of hydroxyproline, and protect against the development of pulmonary fibrosis, which together led to an increase in the animal survival. Moreover, blocking IL-17A decreased the expression ofpro-fibrotic cytokines such as IL-17A, TGF-beta1 and IL-13; increased the expression of anti-fibrotic or anti-inflammatory factors such as IFN-gamma, COX-2, 5-LOX, 15-LOX. Indeed, IL-17A antagonism suppressed the activation of pro-inflammatory p65NF-kappaB but enhanced the activation of pro-resolving p50NF-kappaB. In conclusion, that blockade of IL-17A prevents the development of pulmonary fibrosis from acute lung injury, is because blocking IL-17A may prevent acute inflammation converting to chronic inflammation.

Objective:Comparing the effect of different doses of simvastatin in lowering the serum lipid.Methods:79 patients were randomized into group A and group B,and were given simvastatin 10 mg*d-1 (group A) and 20 mg*d-1 (group B),respectively for a total of 8 weeks.Results:Comparing with baseline,in group A,TC,TG,LDL-C were decreased by 23.4%,20.0% and 30.7%,respectively (P＜0.01); HDL-C was increased by 17.5%.The content of serum TC,TG and LDL-C was decreased to the normal range in 12.8%,28.2% and 15.4% of the patients in group A.For the group B,TC,TG,LDL-C were decreased by 32.7%,22.8% and 42.8%,respectively (P＜0.01); HDL-C was increased by 13.7%.The content of serum TC,TG and LDL-C was decreased to the normal range in 65.0%,57.5% and 65.0% of the group B patients.Conclusion:Oral intake of 20 mg of simvastatin once a day can effectively reduce the serum lipid.The patients can well tolerate and no obvious side effect was observed in our study.

ObjectiveTo explore the clinical effect of omeprazole combined with octreotide in treatment of patients with non-variceal upper gastrointestinal hemorrhage.Methods96 patients with non-variceal upper gastrointestinal hemorrhage were randomly divided into two groups.All the cases were received basic treatment of fluid infusion,transfusion and nutritional support.The control group( n =48) was treated with omeprazole alone,and the treatment group(n =48) was treated with omeprazole and octreotide.The course of treatment was 3 days.The vital signs,24h urine output,the number of cases of rebleeding in 72 h and adverse drug reactions was observed and recorded.ResultsThe total effective rate in treatment group and control group was 91.7％ and 72.9％,respectively.The difference between the two groups was statistically significant( x2 =5.79,P ＜0.05 ).The time of hemostasis and blood transfusion volume in treatment group were significantly less than those in control group(t =7.69,9.91,all P ＜0.05).The rebleeding rates after 72 hours of hemostasis between the two groups(8.3％ vs 25.0％ ) was significantly different ( x2 =4.80,P ＜ 0.05 ).In the course of treatment,the side effects weren' t found in both groups.ConclusionOmeprazole combined with octreotide was more effective and safe than omeprazole alone in fast stopping bleeding and reducing rebleeding rate.

Objective To evaluate the efficacy and safety of cinacalcet on secondary hyperparathyroidism (SHPT) in patients with end-stage renal disease (ESRD). Methods Patients with ESRD and SHPT for the treatment with calcimimetic agents were included in this study. MEDLINE (1996.1-2014.9), OVID (1963.1-2014.9), Chinese Wanfang database (1996.1-2014.9), CNKI (1996.1-2014.9) and the clinical control test database of Cochrane Library were searched . Related literature, including published or unpublished papers, and meeting procedding were hand-searched. Quality assessment and data extraction were conducted by two independent investigators. Meta-analysis was conducted by RevMan 5.2. Results Nineteen randomized controlled trials involving 7 702 patients were included. The meta-analysis showed that compared with conventional therapy,cinacalcet can significantly decrease serum parathyroid hormone in dialysis patients [WMD=-301.54 μg/L, 95%CI:(-344.38)-(-258.7)μg/L, P<0.05], decrease serum level of calcium [WMD=-8.3 mg/L, 95%CI:(-9.1)-(-7.4)mg/L, P<0.05], and decrease serum level of phosphorus [WMD=-3.4 mg/L, 95%CI:(-4.6)-(-2.3) mg/L, P<0.05]. The total incidence of adverse events was similar (RR=1.03, 95%CI:0.98-1.09, P>0.05). Cinacalcet increased nausea (RR =2.05, 95%CI :1.53-2.75, P<0.05), vomiting (RR =2.00, 95%CI:1.78-2.23, P<0.05), diarrhea (RR=1.15, 95%CI:1.03-1.30, P<0.05), and asymptomatic hypocalcaemia (RR=7.60, 95%CI:5.61-10.30, P<0.05), but they were usually transient, and mild to moderate in severity. The mortality was similar (RR=0.97, 95%CI:0.89-1.05, P>0.05). Conclusion Results confirm that cinacalcet suppresses parathyroid hormone and decreases calcium and phosphorus in secondary hyperparathyroidism patients receiving dialysis. Cinacalcet increases risks of nausea, vomiting, diarrhea and hypocalcaemia,without increasing mortality.

Objective To assess the effect of hemoperfusion (HP) and hemodiafiltration (HDF) on elimination of para?thyroid hormone (PTH) from plasma in long-term hemodialysis patients.Methods MEDLINE (1966—2013.8), Embase (1974—2013.8), Chinese Wanfang database (1996—2013.8),CNKI(1979—2013.8)and the clinical control test database of Cochrane Library were searched. Published reports, unpublished studies and abstract from conference were retrieved manu?ally, all of which include studies of effect of PTH elimination through hemoperfusion and hemodiafiltration. Quality assess?ment and data mining were conducted by two independent investigators who performed meta-analysis using RevMan 5.2. Re?sults A total of 12 reports (400 patients) met the inclusion criteria and were included in this study. The meta-analysis showed that the elimination rate of PTH in long term hemodialysis patients was similar between using hemoperfusion and us?ing hemodiafiltration(WMD=-0.34,95%CI:-12.66-11.98,P=0.96). What’s more the elimination rate ofβ2-MG were also similar(WMD=-2.92,95%CI:-16.64-10.8,P=0.68)between those two treatments. However,The remission rate of pruritus was higher in patients using HP group than in HDF group(RR=1.33,95%CI:1.08-1.62,P=0.006). Conclusion HP and HDF can effectively clear PTH in chronic hemodialysis patients, but there was no statistically different between the two treat?ment methods.

Objective To investigate the clinical effect of treatment with local skin flap after primary surgical resection of non melanoma skin cancer.Methods A total of 176 patients with head and facial skin cancer who were admitted into our hospital from September 2008 to September 2015 were divided into three groups according to different repair plans.Namely,the suture group (41 cases),the local skin flap group (81 cases) and the skin graft group (54 cases).The repair effect of the three groups were compared after the operation.Results The good rate was 68.39%(28/41) in the suture group,98.77%(80/81) in the local skin flap group,and 83.33%(45/54) in the skin graft group,and it was significantly higher than any of the other two groups with statistically significant difference(P<0.05).The satisfaction rate of the local skin flap group was significantly better than that of the suture group,and the difference was statistically significant(P<0.05).But there was no significant difference in the satisfaction rate when compared with the skin graft group(P>0.05).The 3 groups were followed up for a period of 1 year,as a result,there was no recurrence and distant metastasis.Conclusion For patients with head and facial non melanoma skin cancer,repaired with local skin flap healing treatment after primary surgical resection can not only get high healing rate but also significant improvement of patient satisfaction rate.In addition,it can lower the rate of recurrence and distant metastasis after operation.