Objective To explore the relationship of metabolic syndrome(MS)with body mass index(BMI)and waist circumference(WC)in residents of urban and rural in Guangxi.Methods According to the method of multistage stratified randomized cluster sampling confirmed in ″The Nutrition and Health Status of the Chinese People,2002″,2 970 urban and rural people(aged ≥18 years)were selected from 4 cities and 4 countries in Guangxi and their body height,weight,waist circumference and laboratory tests were investigated.Results The prevalence rate of MS was 3.77%,and the urban rate(5.56%)was higher than that of the rural(2.05%),male's rate(4.99%)was higher than female's(2.68%).The prevalence rates of MS in groups with BMI

Objective To explore the relationship between nutrients intake and the prevalence of metabolic syndrome(MS) in urban and rural residents in Guangxi.Methods 3026 people aged 18 years and above were sampled at random from 4 cities and 4 countries in Guangxi with a multistage stratified sampling.All subjects took the diet investigation with 24-hours review method,medical examination and blood assay.Results 107 subjects(3.54%) suffered from MS,and the prevalence rate in city(5.21%) was significantly higher than that in countryside(1.90%,P

Objective To analyze the drug resistance of Escherichia coli isolated from urinary tract infection and risk factors of quinolone resistance strains.Methods A total of 705 cases (strains) with Escherichia coli drug resistance isolated from urine specimens were divided into quinolone sensitive group [474 cases(strains)] and quinolone resistance group [231 cases(strains)].The risk factors of the quinolone resistance strains were analyzed.Results The sensitivity rate of amoxicillin/clavulanic acid,cefalotin,ceftazidime,aztreonam,piperacillin,amikacin,compound sulfamethoxazole,ciprofloxacin,gentamicin,levofloxacin,cefepime in quinolone resistance group was higher than that in quinolone sensitive group [50.2％(238/474) vs.78.8％(182/231),11.6％(55/474) vs.48.5％(112/231),17.9％(85/474) vs.63.2％(146/231),15.0％(71/474) vs.57.6％(133/231),3.2％(15/474) vs.27.7％(64/231),80.8％(383/474)vs.93.1％(215/231),16.0％(76/474) vs.49.8％(115/231),0 vs.100.0％(231/231),32.5％ (154/474)vs.70.6％ (163/231),3.8％ (18/474) vs.98.7％ (228/231),18.6％ (88/474) vs.63.2％ (146/231),P ＜0.05].Logistic regression analysis showed history of using the third generation cephalosporins and quinolones,urinary drainage and bacterium producing extra-broad spectrum beta-lactamase was independent risk factor for quinolone resistance Escherichia coli (P ＜ 0.05).Conclusions The epidemic of quinolone resistance Escherichia coli isolated from urine specimens is extremely serious.The quinolone resistance is strong,and infection patients have a high medical cost and average length of stay.The quinolone resistance Escherichia coli infection has multiple independent risk factors.To strengthen the control of the independent risk factors can effectively prevent quinolone resistance strains infection spread.

Objective To establish and apply the multiple probe RT-PCR analysis for H5N1 pathogen detection methods. Methods Used the gene sequences published in GenBank,which were designed and synthesized two pairs of specific primers (P1/P2,P3/P4)accorded the conserved regions.Through the optimization of amplification conditions to establish the rapid detection of two viruses duplex PCR method.Results The suitable primer concentration:P1/P2 was 0.32μmol/L,P3/P4 was 0.96μmol/L.The amount of the minimum virus nucleic acid was 2 ng/μl by the double detect,H5N1 pathogen mixed were occurred specific bands in the corresponding location,and other viruses and blank control strips had not bands.Conclu-sion A variety of probes for RT-PCR analysis H5N1 pathogen detection methods in a reasonable application of primer con-centration under has good sensitivity and specificity,and it is worth the inspection application.

Objective To investigate folic acid combined with Qizhi Weitong granule on serum gastrin, sIL-2R and immune function in chronic atrophy gastritis patients of Hp positive.Methods 46 cases with Hp positive chronic atrophy gastritis were selected and randomly divided into two groups according to different treatment, 23 cases in each group.The control group were given folic acid tablet on the basis of conventional treatment, and the experimental group were treated on the basis of control group combined with Qizhi Weitong granule.The patients in two groups were all treated for three months.Serum gastrin, sIL-2R and T cell subsets levels were detected after treatment.ResuIts Compared with control group after treatment, the serum gastrin level of experimental group was higher (P<0.05), sIL-2R level was lower (P<0.05), CD4 +T, CD4 +T/CD8 +T were higher (P<0.05), and CD8 +T level was lower ( P<0.05 ) .ConcIusion Folic acid combined with Qizhi Weitong granule could significantly increase serum gastrin and CD4 +T level and reduce sIL-2R and CD8 +T level in chronic atrophy gastritis patients of Hp positive, as a guidance for clinical.

Objective To discuss the clinical characteristics for central compartment lymph node metastasis in stage cNO papillary thyroid microcarcinoma and significance and feasibility of preventive dissection,aimed to provide reference for clinical treatment.Methods Reviewed the clinical data of 277 patients with stage cNO papillary thyroid microcarcinoma from Jul.2011 to Dec.2015 underwent surgery in the Department of General Surgery of Lianyungang East Hospital.Evaluated the necessity of prophylactic central lymph node dissection.Adopted chi square test and Logistic regression to analyze its relationship with patients' gender,age,tumor number,tumor size,enveloped infiltration,single and bilateral tumor.All 277 patients underwent primary radical resection with ipsilateral central lymph node dissection.The specimen of resection was analyzed by routine pathology.Results The positive rate of thyroid papillary microcarcinoma lymph node metastasis was 36.8 ％ (102/277).The elements of male patients (P =0.023),age ＜ 45 years (P ＜ 0.001) and tumor diameter ＞ 0.5 cm (P =0.019)had high positive rate.The multivariable analysis showed that male patients (OR =2.63,P ＜ 0.001),age ＜ 45 years (OR =2.25,P =0.016),tumor diameter ＞ 0.5 c m (OR =2.13,P =O.009) were independent risk factors for CLN metastasis.Forty-three (15.5％) cases had transient parathyroid function.No Permanent recurrent nerve paralysis and hyperparathyroidism occurred in this group.Conclusions Prophylactic central lymph node dissection is helpful for accurate staging of tumor classification and risk assessment,has important significance,on the follow-up of patients after treatment of choice.For male patients,age ＜ 45,tumor diameter ＞ 0.5 cm,enveloped infiltration,the central compartment lymph node dissection may be necessary.

Objective To evaluate the effect of double stapling technique for sphincter preservation operation in mediate-low rectal carcinoma.Methods The resection through double stapling technique for sphincter preservation approach was performed on 30 patients with mediate-low rectal carcinoma from January 2004 to November 2007.The distance between the anal verge and the lower margin of the tumor was 4～8cm(averaged 6.5 cm),including 22 patients in Dukes A stage,6 Dukes B and 2 Dukes C.Results Fecal continences were preserved successfully in all patients.2 cases had got anastomotic narrowing(6.6%) after operation.At a median follow-up of 46 months,two patients had local recurrences(6.6%).There was no urinary dysfunction in this series.Conclusion Double stapling technique for sphinceter preservation operation was feasible and safe,provide a original operative style for mediate-low rectal carcinoma.

Objective To evaluate the effect of procaine on prevention of skin slap necrosis after modified radical mastectomy in breast cancer.Methods 106 breast cancer patients were randomly divided into treatment and control groups.In the treatment group,procaine(1% 100ml diluted in 300ml mormal saline,42℃)were compressed under the skin flap in operation,the control group received same amount of normal salin instead.The rates and sizes of skin flap necrosis were obeserved and compared.Results There were 13 cases suffured from skin flap necrosis among 106 breast cancer cases who received surgery operation.The rate of the flap necrosis in treatment group was5.76%(3/52),and the control group was 18.51%(10/54),there was significant difference(P

ObjectiveTo study the effect of berberine on diabetes or insulin resistance accompanied with reduction of skeletal moscle and wasting in db/db mice.Methods db/db mouse-a model of diabetes/insulin-resistance was studied,with the wild type mouse as control.After being treated with berberine ( 5 mg · kg-1 · d-1 ) for 3 weeks,the muscle size of tibia anterior (TA) of the animals was measured after staining with laminine/Hyosin Heavy chain-Slow using immunochemistry,then observed under fluorescent microscope and calculated with software.The rate of [ 14 C ] -Phenalanine incorporation into the uuscle was measured to analyze the protein synthesis,and the [ 3 H ] -Tyrosine released into the medium was determined in order to analyze protein degradation.The mRNA expressions of muscle atrophy Fbox-1 ( Atrogin-1 ) and muscle ring finger-1 ( MuRF-1 ) were measured by Northern blot.Results With berberine treatment,blood glucose and fat levels were lowered [ ( 18.55 ± 3.79 vs 26.32 ± 4.02 ) mmol/L,P＜0.01 ; ( 2.75 ± 0.30 vs 3.77 ± 0.52 ) g,P＜0.05 ],but tibia anterior muscle weight/length ratio and cross-section area were decreased,rates of protein synthesis in isolated muscles of db/db mice were decreased by 18％ -22％,and the rates of degradation were significantly raised by 24％ -26％ after berberine treatment.There also was increased the transcription and translation of Atrogin-1 and MuRF-1,accompanied with decreased eukaryotic initiation factor 3 subunit (eIF3-f)protein level simultaneously. ConclusionBerberine improves hyperglycemia and insulin resistance by down-regulating blood sugar and body fat,but it causes reduced protein synthesis and minimally enhanced protein degradation.The mechanism might be related to berberine-induced up-regulating Atrogin-1 、MurF-1 and down-regulating eIF3-f.

To provide a reference basis for the structural design and optimization of endovascular stent by analyzing the flow resistance of the new stent with triangle cross-section using numerical simulation method, and finding out the stent structure which will influence hemodynamic parameters. Four kinds of models of the triangle cross-section bare stent were constructed in infinite flow field using solidworks software. Numerical simulations of the four models were performed respectively using ANSYS finite element software. Steady flows and transient flows in these models were studied. Hemodynamics data in the four models were collected, such as the flow patterns, the distribution of pressure and the flow resistance of the new stents. The stent with triangular wire cross-section can be applied to cover aneurysm cavity because that resistance of blood inflow to aneurysm was large and outflow from aneurysm was small. Thus, it was easy to outflow than inflow. Thereby blood perfusion and flow of aneurysm cavity was restrained, and the pressure of aneurysm cavity was reduced, which plays a certain effect on the treatment of aneurysm. This result provides some instructions for the design of stent structure.