Objective To establish and apply the multiple probe RT-PCR analysis for H5N1 pathogen detection methods. Methods Used the gene sequences published in GenBank,which were designed and synthesized two pairs of specific primers (P1/P2,P3/P4)accorded the conserved regions.Through the optimization of amplification conditions to establish the rapid detection of two viruses duplex PCR method.Results The suitable primer concentration:P1/P2 was 0.32μmol/L,P3/P4 was 0.96μmol/L.The amount of the minimum virus nucleic acid was 2 ng/μl by the double detect,H5N1 pathogen mixed were occurred specific bands in the corresponding location,and other viruses and blank control strips had not bands.Conclu-sion A variety of probes for RT-PCR analysis H5N1 pathogen detection methods in a reasonable application of primer con-centration under has good sensitivity and specificity,and it is worth the inspection application.

Objective To investigate folic acid combined with Qizhi Weitong granule on serum gastrin, sIL-2R and immune function in chronic atrophy gastritis patients of Hp positive.Methods 46 cases with Hp positive chronic atrophy gastritis were selected and randomly divided into two groups according to different treatment, 23 cases in each group.The control group were given folic acid tablet on the basis of conventional treatment, and the experimental group were treated on the basis of control group combined with Qizhi Weitong granule.The patients in two groups were all treated for three months.Serum gastrin, sIL-2R and T cell subsets levels were detected after treatment.ResuIts Compared with control group after treatment, the serum gastrin level of experimental group was higher (P<0.05), sIL-2R level was lower (P<0.05), CD4 +T, CD4 +T/CD8 +T were higher (P<0.05), and CD8 +T level was lower ( P<0.05 ) .ConcIusion Folic acid combined with Qizhi Weitong granule could significantly increase serum gastrin and CD4 +T level and reduce sIL-2R and CD8 +T level in chronic atrophy gastritis patients of Hp positive, as a guidance for clinical.

Objective To explore the relationship of metabolic syndrome(MS)with body mass index(BMI)and waist circumference(WC)in residents of urban and rural in Guangxi.Methods According to the method of multistage stratified randomized cluster sampling confirmed in ″The Nutrition and Health Status of the Chinese People,2002″,2 970 urban and rural people(aged ≥18 years)were selected from 4 cities and 4 countries in Guangxi and their body height,weight,waist circumference and laboratory tests were investigated.Results The prevalence rate of MS was 3.77%,and the urban rate(5.56%)was higher than that of the rural(2.05%),male's rate(4.99%)was higher than female's(2.68%).The prevalence rates of MS in groups with BMI

Objective To explore the relationship between nutrients intake and the prevalence of metabolic syndrome(MS) in urban and rural residents in Guangxi.Methods 3026 people aged 18 years and above were sampled at random from 4 cities and 4 countries in Guangxi with a multistage stratified sampling.All subjects took the diet investigation with 24-hours review method,medical examination and blood assay.Results 107 subjects(3.54%) suffered from MS,and the prevalence rate in city(5.21%) was significantly higher than that in countryside(1.90%,P

Objective To analyze the drug resistance of Escherichia coli isolated from urinary tract infection and risk factors of quinolone resistance strains.Methods A total of 705 cases (strains) with Escherichia coli drug resistance isolated from urine specimens were divided into quinolone sensitive group [474 cases(strains)] and quinolone resistance group [231 cases(strains)].The risk factors of the quinolone resistance strains were analyzed.Results The sensitivity rate of amoxicillin/clavulanic acid,cefalotin,ceftazidime,aztreonam,piperacillin,amikacin,compound sulfamethoxazole,ciprofloxacin,gentamicin,levofloxacin,cefepime in quinolone resistance group was higher than that in quinolone sensitive group [50.2％(238/474) vs.78.8％(182/231),11.6％(55/474) vs.48.5％(112/231),17.9％(85/474) vs.63.2％(146/231),15.0％(71/474) vs.57.6％(133/231),3.2％(15/474) vs.27.7％(64/231),80.8％(383/474)vs.93.1％(215/231),16.0％(76/474) vs.49.8％(115/231),0 vs.100.0％(231/231),32.5％ (154/474)vs.70.6％ (163/231),3.8％ (18/474) vs.98.7％ (228/231),18.6％ (88/474) vs.63.2％ (146/231),P ＜0.05].Logistic regression analysis showed history of using the third generation cephalosporins and quinolones,urinary drainage and bacterium producing extra-broad spectrum beta-lactamase was independent risk factor for quinolone resistance Escherichia coli (P ＜ 0.05).Conclusions The epidemic of quinolone resistance Escherichia coli isolated from urine specimens is extremely serious.The quinolone resistance is strong,and infection patients have a high medical cost and average length of stay.The quinolone resistance Escherichia coli infection has multiple independent risk factors.To strengthen the control of the independent risk factors can effectively prevent quinolone resistance strains infection spread.

Objective To explore the clinical significance of the union between marrow smear and marrow biopsy in the myelodysplastic syndrome(MDS) diagnosis. Methods Bone marrow aspirate and smear were initially abtained, then bone tissues encircled drill and section at the same point which is called as. easy one-step technology to 86 MDS patients were analysed. Results In 86 cases of MDS patients, there were 30 cases of hyperplasia extreme degree of reduction by 34.88 %, 56 eases of active, obvious and extremely active active (65.12 %), 43 cases for red RCMD (50.00 %), 32 cases for the granulocyte dysplasia (37.21%), 22 cases for megakaryocyte RCMD (25.58 %) in bone marrow aspiration smears; compared with 15 cases of hyperplasia extreme degree of reduction and the reduction (17.44 %), 71 eases of active, obviously active and extremely active (82.56 %); 16 cases for red RCMD (18.61%), 52 cases for the granulocyte dysplasia (60.47 %), 56 cases for megakaryocyte RCMD (65.12 %) in bone marrow biopsy sections. 66 cases in 86 cases of bone marrow biopsy and bone marrow smear of WHO classification were in line with the rate of 76.74 %.Conclusion The biopsy slide and the puncture smear synchronization observation is more advantageous than the conventional puncture smear morphology observation and combining two method may increase the accuracy in the MDS diagnosis.

ObjectiveTo study the effect of berberine on diabetes or insulin resistance accompanied with reduction of skeletal moscle and wasting in db/db mice.Methods db/db mouse-a model of diabetes/insulin-resistance was studied,with the wild type mouse as control.After being treated with berberine ( 5 mg · kg-1 · d-1 ) for 3 weeks,the muscle size of tibia anterior (TA) of the animals was measured after staining with laminine/Hyosin Heavy chain-Slow using immunochemistry,then observed under fluorescent microscope and calculated with software.The rate of [ 14 C ] -Phenalanine incorporation into the uuscle was measured to analyze the protein synthesis,and the [ 3 H ] -Tyrosine released into the medium was determined in order to analyze protein degradation.The mRNA expressions of muscle atrophy Fbox-1 ( Atrogin-1 ) and muscle ring finger-1 ( MuRF-1 ) were measured by Northern blot.Results With berberine treatment,blood glucose and fat levels were lowered [ ( 18.55 ± 3.79 vs 26.32 ± 4.02 ) mmol/L,P＜0.01 ; ( 2.75 ± 0.30 vs 3.77 ± 0.52 ) g,P＜0.05 ],but tibia anterior muscle weight/length ratio and cross-section area were decreased,rates of protein synthesis in isolated muscles of db/db mice were decreased by 18％ -22％,and the rates of degradation were significantly raised by 24％ -26％ after berberine treatment.There also was increased the transcription and translation of Atrogin-1 and MuRF-1,accompanied with decreased eukaryotic initiation factor 3 subunit (eIF3-f)protein level simultaneously. ConclusionBerberine improves hyperglycemia and insulin resistance by down-regulating blood sugar and body fat,but it causes reduced protein synthesis and minimally enhanced protein degradation.The mechanism might be related to berberine-induced up-regulating Atrogin-1 、MurF-1 and down-regulating eIF3-f.

To provide a reference basis for the structural design and optimization of endovascular stent by analyzing the flow resistance of the new stent with triangle cross-section using numerical simulation method, and finding out the stent structure which will influence hemodynamic parameters. Four kinds of models of the triangle cross-section bare stent were constructed in infinite flow field using solidworks software. Numerical simulations of the four models were performed respectively using ANSYS finite element software. Steady flows and transient flows in these models were studied. Hemodynamics data in the four models were collected, such as the flow patterns, the distribution of pressure and the flow resistance of the new stents. The stent with triangular wire cross-section can be applied to cover aneurysm cavity because that resistance of blood inflow to aneurysm was large and outflow from aneurysm was small. Thus, it was easy to outflow than inflow. Thereby blood perfusion and flow of aneurysm cavity was restrained, and the pressure of aneurysm cavity was reduced, which plays a certain effect on the treatment of aneurysm. This result provides some instructions for the design of stent structure.

To evaluate the effects of supplemental probiotics and xylo-oligosaccharide on performance,digestive enzyme activities,blood index and intestinal microflora.Two hundred and forty crossbred early weaned PIC piglets with an average initial weight of (6.83 + 0.9) kg,weaned at (21 ± 2) d of age,were divided into four groups.Control Ⅰ (positive diet,with no probiotics and xylo-oligosaccharide but more fish meal,whey powder and little soybean meal),Control Ⅱ (negative diet,with no probiotics and xylo-oligosaccharide but less fish meal,whey powder and more soybean meal),Negative diet + 0.035 % probiotics,negative diet + 0.002% xylo-oligosaccharide.The results of theses studies suggested that whey powder and fish meal from 5.50% to 2.50%,soybean meal from 22.50% to 26.50% could result in decreasing of alimentary canal enzyme activity,blood sugar,total serum protein and the quantities of Bacillus in appendix(P<0.05),and raising of serum urea nitrogen,the quantities of Escherichia coli in appendix,diarrhea indexes and Feed/Gain(P<0.05).Addition of probiotics and xylo-oligosaccharide could significantly improve the activities of alimentary canal protein,lipase and amylase(P<0.05),decrease Feed/Gain and the levels of blood sugar and decrease the levels of serum urea nitrogen and improve protein equilibrium in blood and energy metabolism.The quantities of bacillus were improved,Escherichia coli were decreased in appendix(P<0.05).Diarrhea indexes were decreased(P<0.05).Whether adding probiotics and xylo-oligosaecharide or not could not affect ADG and ADFI (P>0.05).Addition of probiotics and xylo-oligosaccharide could improve digestive function,beneficial microbial population of post-alimentary canal,Feed/Gain and prevent the diarrhea of piglets.

Objective In order to explore the mechanisms of the anti-keratin monoclonal antibody 5G5(MAbSG5)in psoriasis treatment.Methods The different groups of mouse were fed with 5G5.The effect on regulating keratinocyte proliferation was observed.Results The experimental study revealed that MAb5G5 has the function of restraining the karyokinesis of vagina epithelium and accelerating the formation of stratum granulosum in rat squamous epidermis.Conclusion The mechanism of MAb5G5 in psoriagis treatment may be related to regulate keratinocyte proliferation.