Objective:To clone human interleukin-33(hIL-33)and express it in E.coli efficiently.Methods:The primers were synthesized according to the hlL-33 cDNA sequence in GeneBank.The hIL-33 was amplified by RT-PCR from human fibroblast cell line(L929),the PCR product was inserted into pUC19 vector.The IL-33 cDNA confirmed by sequencing was inserted into expressing vector PQE30 and expressed in E.coli M15 strain.IL-33 protein expression was induced by IPTG and purified by Ni-NTA affinity chromatography.The recombinant IL-33 was identified by Immunoblot and its biological activity was analyzed.Results:DNA sequencing confirmed that the cloned cDNA was identical to the published sequence of hIL-33.The recombinant plasmid PQE/hIL-33 was transformed into M15.An expected 18KD protein of hIL-33 found mainly in the induced host strains about 25% of total bacteria lysis by SDS-PAGE and coomassie blue staining.The 18 KD protein could be recognized by anti-IL33 antibody in western blot.The recombinant protein was purified to more than 95% of total protein and induces the production of IL-4 and IL-5 in human peripheral blood mononuclear cells.Conclusion:We have successfully expressed hIL-33 protein in E.coli and the expressed product has IL-33 specific bioactivity.

AIM: To investigate the pentanucleotide repeat(PNR) polymorphism of apolipoprotein(a) in patients with coronary heart disease(CHD) in Hubei area, and evaluate the association of polymorphism of apo(a) PNR with the level of serum lipid. METHODS: Objects examined were composed of two groups: 88 patients with CHD and 153 healthy controls. Lp(a), TC,TG, HDL-C, LDL-C, ApoAⅠand ApoB of two groups were tested. Meanwhile,the PNR in the 5' control region of the Apo(a) was detected by using polymerase chain reaction (PCR) and high voltage polyacrylamid gels electropherosis. RESULTS: The serum Lp(a), TC, TG and LDL-C levels were remarkably higher in the CHD than that in control( P

BACKGROUND: Apolipoprotein(a) [Apo(a)] plays some role in promoting the formation of atherosclerotic plaque, and contains pentanucleotide repeats(PNR), which has a key value in genic research and in forecast on the increased risk of early atherosclerosis cerebral infarction (ACI). But the relationship between ACI and Apo(a) PNR in different races needs to be further investigated.OBJECTIVE: To investigate the relationship between the Apo(a) PNR polymorphism and ACI.DESIGN: A case-control study based on the ACI patients and normal people of Han nationality in Hubei.SETTING: Department of Laboratory in a hospital of a university.PARTICIPANTS: From February 1998 to March 1999, 82 ACI patients (ACI group) and 153 healthy controls(control group) were selected from the Department of Neurology, Central South Hospital and Yatai Hospital of Wuhan University. All patients were Han nationality in Hubei without any relatives.METHODS: Serum lipoprotein(a) [Lp(a) ], total cholesterol(TC), high density lipoprotein cholesterol(HDL-c), low density lipoprotein cholesterol (LDL-c), triglyceride (TG), apolipoprotein A Ⅰ (ApoA Ⅰ) and apolipoprotein B (Apo B) were tested respectively. Meanwhile, the PNR in the 5' control region of the Apo(a) was detected with polymerase chain reaction(PCR) and high voltage polyacrylamide gels electrophoresis. The results were analyzed with controlled analysis.RESULTS: The levels of serum Lp(a) [ (239.9 ±225.4) mg/L], TC [(4.76±0.74) nmol/L], TG[(1.74±0.60) mmol/L] and LDL-C [ (2.84 ± 0. 63) mmol/L] were remarkably higher in ACI group than those in control group, which were(133.5 ±97.7) mg/L in serum Lp(a), (4. 29±0.72) mmol/L in TC, (1.05±0.52) mmol/L in TG and(2.84±0.63) mmol/L in LDL-C, however, the level of HDL-C[ (0.88± 0.17) mmol/L] was remarkably lower in ACI group than that in control group [ ( 1.03 ± 0. 35 ) mmol/L], the differences were all significant( t = 3.65to9.18, P ＜ 0.01) . The levels of ApoA Ⅰ [(1.13±0.15) mmol/L,(1.25±0.19) mmol/L] and ApoB[(0.93±0.12) mmol/L, (0.89± 0. 15 ) mmol/L] were no significant difference compared with those in control group. The duplicated frequency of the allele(TTTTA) 5(0. 098) in the ACI was remarkably higher than that in control(0. 026) (x2 = 5.62, P＜ 0. 05), The frequency of the allele(TTTTA) 9 in the ACI(0. 073) was remarkably lower than that in control (0. 213 ) (x2 = 7.83, P ＜ 0.01 ), The frequency of the allele(TTTTA) 5 was also associated with low TC and high Lp(a) levels.CONCLUSION: It is suggested that the Apo(a) PNR polymorphism are associated with the susceptibility to ACI, and involved in the development of ACI.

Objective To understand the characteristics of High-density lipoprotein cholesterol (HDL-C) distribution through analyzing serum HDL-C levels in healthy checkup subjects among local urbanese.Methods The checkup results of 36 454 cases were collected from 2009 to 2013 in Tangshan Gongren hospital (male:n =20 343,female:n =16 111).The cases with liver injury,abnormal blood glucoses,kidney injury and defined cerebrovascular diseases and metabolic disease were excluded.25 197 cases were analyzed as normal subjects including 11 114 males and 14 083 females.Kolmogorov-Smimov test,kruskal-wallis test,and Dunn multiple comparison test was performed using Bioconductor software 3.0.2 for testing normality distribution and comparing the difference of two or multiple groups,respectively.Results were analyzed statistically with R 3.0.2.Results The results showed that the average level (1.22-± 0.31) mmol/L of HDL-C among the overall population is lower than that of national average level(1.30 mmol/L).The median serum HDL-C level in female is higher than in male (1.27 and 1.08,x2 =2 606.34,P ＜0.01).HDL-C levels in male continuously increase from 1.06 mmol/L to 1.11 mmol/L with aging,especially in groups of over 50 years old than in groups of below 50 years old (x2 =75.19,P ＜ 0.01).Conclusions Based on 2007 guidance on prevention and treatment by national health bureau,this study showed that there are 29.69％ of the apparent healthy subjects,especially about 42.94％ of the male,representing low HDL-C level under the low limit of 1.04 mmol/L.These results showed that serum HDL-C level in Tangshan urbanese is lower than that of national average level,and HDL-C level in male is tended to increase with aging.

Objective To investigate the features of epileptic seizures and eletroencepalogram (EEG) in patients with mitochondrial myopathy encephalopathy,lactic acidosis and stroke-like episodes (MELAS).Methods Forty-four patients with MELAS were diagnosed at the First Hospital of Peking University from November 2007 to August 2013.EEG and head MRI were performed on all patients.The types of epileptic seizure and EEG changes were compared between patients in and outside stroke-like episodes.Results Epileptic seizures occurred in 39 of 44 patients (88.6％) with MELAS,while multitype epileptic seizures were presented in 26 cases (66.7％).In stroke-like episodes,22 patients presented with partial seizures,30 with generalized seizures and 17 with status epilepticus.In nonstroke-like episodes,7 patients presented with partial seizures,14 with generalized seizures and 2 with status epilepticus.The frequency of partial seizures,generalized tonic-clonic seizures,status epilepticus were 47.7％ (21/44),68.2％ (30/44),38.6％ (17/44) in stroke-like episodes and 13.6％ (6/44),27.3％ (12/44),4.5％ (2/44) in nonstroke-like episodes,which have statistical significance (x2 =12.022,14.758,15.103;P =0.001,0.000,0.000,respectively).Abnormal EEGs appeared in all patients.The rates of slow alpha frequency,diffuse δ or θ wave,epileptic discharges were 6.8％ (3/44),43.2％ (19/44),25.0％(11/44) in stroke-like episodes and 31.8％ (14/44),59.1％ (26/44),22.7％ (10/44) in nonstroke-like episodes,respectively.Slow alpha frequency were significantly different between patients in and outside stroke-like episodes (x2 =8.822,P =0.003).Conclusions Epileptic seizures with different types are more common during stroke-like episodes in patients with MELAS.While the rates of epileptic discharges are also common outside the stroke-like episodes.

OBJECTIVE: Analyze the ABR testing results of local infants who failed the hearing screening and summarize its characteristics. METHOD: Carry on the first ABR test to 140 infants who failed the newborns' hearing screening when they are about 3-month age, the unusualities carry on the second ABR test at about 6-month age, and carry on the statistical analysis. RESULT: One hundred and forty infants with the first ABR diagnosis, 65 infants are normal, accounting for 46.43%; 75 infants have single or binaural ear hearing disorder, accounting for 53.57%, single ear 17.14%, binaural ear 36.43%, of which mild hearing disorder takes most, accounting for 27.14%, the profound hearing loss is also not extremely rare, accounting for 14.29%. Between male and female hearing disorder disease incidence rate difference is not remarkable statistics significance (P > 0.05). Between the number of high-risk in the hearing injury and non-high-risk after the comparison difference is not remarkable statistical significance (P > 0.05). With the population of above severe loss in hearing high-risk has the remarkable statistical significance with the non-high-risk comparison difference (P < 0.05). The second ABR subsequently visit 38 infants, 8 infants normal, total normal rate 52.14% (73/140), 16 infants mild hearing disorder 10 infants transfer normal either the change for the better, 6 infants do not have the change or the aggravation, 3 infants moderate hearing disorder 1 infant changes for the better, 2 infants do not have the change, 9 infants severe hearing disorder 3 infants have the change for the better, 3 infants do not have the change, 3 infants aggravate, 10 infants profound hearing disorder 9 infants do not have the change or the aggravation extremely, 1 example brain paralysis profound hearing disorder extension converges normally extremely. The non-high-risk whose hearing changes for the better is obviously higher than high-risk (P < 0.05). CONCLUSION Should develop newborns' hearing screening comprehensively with key attention to high-risk loss of hearing. The severe hearing disorder has the variability, and so on exceptional child hearing situation appraisal should be prudent to the brain paralysis; should establish perfect tracing, revisiting and managing system as soon as possible.
Evoked Potentials, Auditory, Brain Stem ; physiology ; Female ; Follow-Up Studies ; Hearing Disorders ; prevention & control ; Hearing Tests ; Humans ; Infant ; Infant, Newborn ; Male ; Neonatal Screening

Objective To study the cognition status of the sex attitude and contraceptive knowledge among nurses of low qualification , so as to provide evidence for maintain or promote their reproductive health level.Methods Totals of 120 nurses with work time less than 3 years from 2 grade III hospitals were investigated with self-designed questionnaire .Results Among 120 nurses, 38.8%nurses were much against or against "One night stand".59.0% nurses were opposed to multiple sex partners .44.6% nurses were much against or against early sex involvement or cohabiting .54.7% nurses mastered the contraceptive knowledge . 64.5%nurses knew how to use contraceptive correctly .67.5%nurses knew that they should take contraceptive measures during safe period .74.8%nurses considered that coitus interruptus was not safe .65.5%nurses had not received the special education or training of contraceptive knowledge , and 79.8% nurses were willing to receive the education .93 .4% nurses required the sexual knowledge and would use books or the internet to obtain, while 3.0%nurses would consult others .Conclusions Low qualification nurses have a higher support for the premarital sex , and lack the contraceptive knowledge , so nursing managers should pay more attention to promote their reproductive health .

Objective To investigate the physical constitution and living habits of 800 hypertensive patients by using the current investigation method, and to further explore the relationship.Methods The TCM constitution types and living habits of 800 hypertensive patients who met the diagnostic criteria of hypertension in outpatient and inpatient department of Shaanxi University of Traditional Chinese Medicine from 2014 to 2015 were studied.Results Hypertension patients showed the highest frequency of mediocre, and partial physical constitutionincluded yin deficiency, yang deficiency, phlegm and dampness, qi stagnation, dampness and heat, special temperament and blood stasis. It showed significant difference among the TCM constitution in the fields of age, gender (χ2 values were 40.273, 56.457, 31.719,P<0.01); the coronary heart disease, cerebrovascular disease and blood pressure control were consistent with the distribution of physical types of population, and the difference was significant (χ2 values were 48.975, 8.321, 6.514,P<0.01). In terms of lifestyle, the differences in physical constitution between different tastes were statistically significant (χ2= 480.403,P<0.05); exercise time and sleep (P<0.05), and the difference was significant (P<0.001) the relationship between yin deficiency and exercise time (P<0.001); phlegm-dampness and exercise time and sleep time (P=0.009,P<0.001,P=0.001); qi stagnation and dietary hobby and sleep time (P<0.001); special temperament and diet and sleep time (P values were 0.001, 0.008, respectively).Conclusions The constitution type of TCM in hypertensive patients is related to sex, age, educational level and previous diseases, and is affected by diet, exercise time and sleep habits

Objective:To explore the application effect of PAC psychological resiliency theory combined with sensory integration training in rehabilitation nursing of patients with ischemic stroke.Methods:A total of 102 patients with ischemic stroke who received treatment from January 2017 to November 2019 were selected as research objects by convenient sampling. A total of 51 patients admitted from January 2017 to May 2018 were set as the control group, and 51 patients admitted from June 2018 to November 2019 were set as the observation group. The control group was given routine rehabilitation care, while the observation group implemented nursing intervention based on PAC psychological resiliency theory and sensory integration training at basis of it. The motor function score, activity of daily living score, anxiety score, depression score and nervous system recovery of the two groups before and after intervention were compared.Results:The motor function score of the observation group was higher than that of the control group [ (64.0±14.9) vs. (49.1±13.8) ], and the activity of daily living score was lower than that of the control group [ (13.5±4.4) vs. (17.3±4.0) ] , and the differences were statistically significant ( P<0.05) . The nervous system recovery effect of the observation group was higher than that of the control group, and the difference was statistically significant ( P<0.05) . The anxiety symptom score [ (7.6±2.8) vs. (12.1±2.7) ] and the depression symptom score [ (6.8±2.5) vs. (11.3±2.6) ] of the observation group were lower than those of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:Application of PAC psychological resiliency theory combined with sensory integration training in rehabilitation nursing of patients with ischemic stroke can improve motor function and activity of daily living of patients, reduce anxiety and depression symptoms of them and improve their quality of life.

There is are large number of patients with chronic hepatitis B virus (HBV) infection. HBV not only damages the liver, but also involves the kidney. Hepatitis B virus-associated glomerulonephritis (HBV-GN) is secondary glomerulonephritis caused by HBV infection, and it is one of the most common extrahepatic complications of HBV infection. HBV-GN is mainly observed in children and young and middle-aged adults, with varying degrees of proteinuria as the main clinical manifestation, and it may be accompanied by hematuria and hypertension. Membranous nephropathy is the most common pathological type, followed by membrano-proliferative glomerulonephritis and IgA nephropathy. HBV-GN has an insidious onset and lacks characteristic symptoms and pathological manifestations, and thus it may be easily confused with various types of glomerulonephritis, which may lead to missed diagnosis and misdiagnosis. HBV-GN has a complex pathogenesis involving various links such as immune disorders, direct viral damage, and genetics, among which the theory of immune complex deposition has been widely recognized. In recent years, some important advances have been made in the research on the pathogenesis, diagnosis, and treatment of HBV-GN. This article summarizes the above issues, so as to provide a reference for clinical diagnosis and treatment.