Objective To compare the clinical effect of two ways of the closed reduction internal fixation and open reduction internal fixation in the treatment of joint injury.Methods According to the digital table,84 patients with joint injury were randomly divided into control group and treatment group,42 cases in each group.The control group was given closed reduction and internal fixation,and the treatment group was given open reduction and internal fixation.Results The total time of operation,postoperative hospitalization time,postoperative joint function recovery time of the treatment group were (78.51 ±10.20)min,(13.35 ±2.46)d,(88.43 ±5.27)d,those of the control group were (118.73 ±25.71)min,(19.72 ±3.57)d,(107.42 ±10.33)d,the differences were statistically signifi-cant(t =9.424,9.522,10.613;P =0.000,0.000,0.000).The excellent and good rate of the treatment group was 90.48%,which was higher than 71.43% of the control group,the difference was statistically significant(χ2 =4.941, P =0.026).The treatment group had 1 case of adverse reaction,the control group had 8 cases of adverse reaction, there was statistically significant difference between the two groups(χ2 =6.098,P =0.014).After treatment,the VAS pain score in the treatment group was (2.11 ±0.68)points,which was lower than (4.98 ±1.35)points in the control group,the difference was statistically significant(t =12.305,P =0.000).Conclusion The application effect of closed reduction and internal fixation for patients with joint injury disease is very obvious.

Objective To investigate the protective effects of ulinastatin on the lungs in patients undergoing lung resection after chemotherapy for lung cancer.Methods Thirty ASA Ⅱ or Ⅲ patients with lung cancer(Ⅲ a)aged 54-71yr weighing 55-74 kg undergoing lung resection after chemotherapy were randomly divided into 2 groups(n=15 each):Ⅰ ulinastatin group received ulinastatin 10 000 U?kg~(-1) after induction of anesthesia and Ⅱ control group received normal saline instead of ulinastatin.The patients were premeditated with intramuscular pothidine 70 mg and scopolamine 0.3 mg or atropine 0.5 mg.Anesthesia was induced with midazolam 0.05 mg?kg~(-1),fentanyl 4 ?g?kg~(-1) propofol 0.5-1.0 mg?kg~(-1) and vecuronium 0.1 mg?kg~(-1) and maintained with 1%-2% isoflurane inhalation and vecuronium infusion at 0.06-0.08 mg?kg~(-1)?h~(-1).Blood samples were taken after induction of anesthesia(T_1,baseline),at 40 and 90 rain of one-lung ventilation(T_2,T_3) for determination of serum IL-6,IL-8,IL-10 and TNF-? concentrations.Lung specimen was taken from the operated lung at 90 min of one-lung ventilation for microscopic examination with light and electron microscopo.Results Serum IL-6,IL-8,IL-10 and TNF-? concentrations were all significantly increased during one-lung ventilation as compared to the baseline values at T_1 in both groups.Serum IL-6,IL-8 and TNF-? concentrations were significantly lower while Serum IL-10 concentration was significantly higher in ulinastatin group than in control group during one-lung ventilation(P＜0.05).The histopathologic changes of lung tissue were significantly less in group utinastatin than in group control.Conclusion Ulinastatin can effectively protect the lung in patients with lung cancer after chemotherapy by reducing systemic inflammatory response.

OBJECTIVE: Intraocular lens are applied in trigeminal operations of cataract extraction and comeal transplantation, and the selection of artificial lens is modified. This paper evaluated the application of colodzed polymethyl methacrylate (PMMA) intraocular lens in interlaced non-phacoemulsification cataract extraction with intraocular lens implantation and keratoplasty (trigeminal operation).METHODS: A computer-based online search of CNKI (200312007) and Medline database (1974/2004) was performed for related articles with the key words "colorized intraocular lens, corneal transplantation, cataract, combination operation" in Chinese and "PMMA intraocular lens, cataract extraction, corneal transplantation" in English. Articles related to PMMA intraocular lens in cataract and corneal transplantation trigeminal operation was selected. Meta analysis and repetitive articles were excluded. A total of 11 articles were included and summarized.RESULTS: A total of 21 cases (21 eyes) of corneal leukoma and cataract caused by various trauma or ophthalmopathy underwent colorized PMMA intraocular lens implantation. During the follow-up for 10 months, visual acuities of naked eyes in 92% cases were significantly improved. Two weeks after operations, visual acuities of 9 cases (42.9%) were over 4.7, 10 cases over 4.5, and 2 cases (9.5%) over 4.0. At days 7 to 14 after operation, corneal grafts of 21 cases were completely transparent; 10 months after operation, corneal grafts of 16 cases were completely transparent, 3 cases were semitransparent, and 2 cases were turbid, who underwent second penetrable corneal transplantation.CONCLUSION: The application of colodzed PMMA intraocular lens in penetrable corneal transplantation combined cataract extraction can cure the refracting media turbidity of forepart eye. Compared with traditional operations, trigeminal operation reduces complications, increases safety and improves visual acuity.

Objective To evaluate the effect of irbesartan and nifedipine controlled-release tablets on morning blood pressure surge in patients with essential hypertension. Methods Ninety patients with essential hypertension were randomly divided into irbesartan group ( group A, n = 45) and nifedipine group (group B, n =45). Irbesartan tablets 150 mg/d and nifedipine controlled-release tablets 30 mg/d were respectively given in group A and group B for 4 weeks. The levels of diastolic blood pressure (DBP), systolic blood pressure (SBP), mean arterial pressure ( MAP ) and morning blood pressure surge ( MBPS ) before and after treatment were measured by ambulatory blood pressure monitoring. Results The DBP, SBP, MAP and MBPS levels of two groups after treatment for 4 weeks were all decreased (P < 0.01). All the indexes of group B decreased more significant compared to group A (P < 0.05 ). Conclusion To control blood pressure and prevent morning blood pressure surge, nifedipine controlled-release tablets are better than irbesartan.

Objective To study the probability safety assessment to analyze and evaluate radiation error risk in the external beam radiotherapy,so as to establish and strengthen the control and management of the radiotherapy process,continuous improvement of quality control and quality management.Methods To build the whole of radiotherapy flow chart and process tree,using the decision tree model to determine critical control points in the whole process,making risk assessment chart and analyzing 4 patients with potential safety hazards error.Results The whole process is divided into 22 missions in 3 functional areas,the entire cover 15 branches and 59 key and 11 key control point.The enumeration of error as risks and critical control points has certain correlation.Conclusions Probabilistic safety assessment method have strengthened manage,analyze and control to risk,and all these provide the basis for developing and improving radiotherapy process control management.Radiotherapy quality management for future multidisciplinary and high-level management personnel who take up provides a prospective study.

Objective To explore the Influence of different health education approaches to standard anticoagulation of non-valvular atrial fibrillation. Methods 400 patients with NVAF were randomly divided into the research group (n=200) and the control group (n=200). Baseline clinical information was recorded. Both groups were given regular education and treatment. The control group was randomly divided into two subgroups. One subgroup (patients or/and family members) was given knowledge lectures of atrial fibrillation, atrial fibrillation anticoagulant, and application of warfarin. The other was given the knowledge through booklets. Standard anticoagulation fulfillment rate, ischemic stroke, other events of artery embolism, major bleeding, minor bleeding and death in the two groups were observed after 1 year. The cost for different education approaches was also compared between the 2 subgroups. Results (1)In the research group, standard anticoagulation fullfillment rate (40.53%) was higher than that of the control group(15.96%) (P<0.01). The rate of ischemic stroke (2.63%) was also lower than the control group (4.49%) (χ2=4.49, P<0.05). The death rate was lower in the research group than those in the control group, but minor bleeding rate was higher, without statistically significance (P>0.05).Other artery embolism rate , major bleeding, minor bleeding, death rate had no statistical difference. (2)Knowledge lecture group (10.8±1.0 min) spend more time than the booklet group (1.0±0.5 min) (P<0.01). The booklet group (5.1±1.1 yuan) costed more in terms of money than the knowledge lecture group (1.8±0.5 yuan) (P<0.01). (3)The knowledge lecture group showed higher standard anticoagulation fulfillment rate (47.96%) than that of booklet group (32.60%) (χ2=7.33, P<0.01). There was no statistical difference in ischemic stroke rate, other artery embolism rate, major bleeding rate, minor bleeding rate and the death rate. Conclusions Detailed, correct anticoagulant education and guidance can improve NVAF patients with standard anticoagulation, and reduce the risk of ischemic stroke. Knowledge lecture consumes more time but can improve the standard anticoagulation fulfillment rate.

Objective:To study the diagnostic and therapeutic value of open food challenge in children with cow ′s milk protein allergy.Methods:It is a retrospective analysis of 55 children with suspected cow′s milk protein allergy ( CMPA ) who attended the clinic of the pediatric gastroenterology department ,the First Hospital of Jilin University from March 2014 to March 2015.These children were fed by a mino acid based formulae ( AAF) for 2-4 weeks and then open food challenge ( OFC) test was performed.Those children who tested positive , were diagnosed as having CMPA.They were then fed with AAF for further 3 months and OFC was performed again.Then discusse the diagnostic and therapeutic value of open food challenge in children with cow ′s milk protein allergy.Results:Out of 55 CMPA suspected children ,52 tested positive with OFC yielding a positive rate of 94.55%.These 52 children were fed with AAF for further 3 months and then tested with OFC ,9 children tested positive yielding a positive rate of 17.30%.These 9 children were again fed with AAF for 3 months and then tested with OFC.This time 2 children tested positive yielding a positive rate of 3.85%.Con-clusion:Open food challenge teats are of great significance in the diagnosis of CMPA ,and evaluation of tolerance to cow′s milk protein ( CMP).

Objective To investigate the correlation of serum periostin and severe degree of dilated cardiomyopathy(DCM) in children.Methods Thirty-two children with DCM from Jan.2009 to Jun.2013 in First Hospital Affiliated to Jilin University were selected as DCM group.Then these children were divided into 0-2 score group (n =6),3-6 score group (n =7),7-9 score group (n =11),and 10-12 score group (n =8) according to Ross standardization.Twenty healthy children in the same period were selected as control group.Meanwhile,the levels of serum periostin were detected by using enzyme-linked immunosorbent assay,the levels of serum creatine kinase-MB(CK-MB)were detected by adopting immunosuppressive method,while left ventricular ejection fraction (LVEF) and left ventricular end-diastolic diameter (LVEDD) were observed by using uhrasonography.The data in various groups were compared and the relationship between serum periostin and ROSS,LVEF was analyzed by performing linear regression analysis.Results 1.Compared with control group,the level of serum periostin in DCM group was significantly increased,and the difference was significant (P =0.00).The serum periostin level increased with the increase of ROSS score increase,and there were significant differences among the different ROSS score groups (all P ＜ 0.05).2.Compared with control group,the level of serum CK-MB in DCM group was significantly increased,the difference was significant (P =0.00) ;while the serum CK-MB levels had no significant difference among the different ROSS score groups(all P＞0.05).3.Compared with control group,the level of LVEF in DCM group was significantly decreased,the differences was significantly(P =0.00) ; and the LVEF level decreased with the ROSS score increase,there were significant differences among the different ROSS score groups(all P ＜ 0.05).4.Compared with control group,the level of LVEDD in DCM group was significantly enlarged,and the difference was significant(P =0.00) ; but the LVEDD had no significant differences among the different ROSS score groups (all P ＞ 0.05).5.Significantly positive correlation was found between the level of serum periostin and ROSS score (r =0.742,P ＜ 0.001).There was no obvious correlation between the level of serum periostin and CK-MB(r =0.247,P ＞ 0.05).There was negative correlation between the level of serum periostin and LVEF (r =-0.424,P ＜ 0.01).Conclusions The level of serum periostin is positively correlated with ROSS score and has negative correlation with LVEF.It may provide a useful index in the assessment of symptomatic state in children with dilated cardiomyopathy.

Objective To explore the preventive effect of Bailing capsules(BL) on myocardial fibrosis in the viral myocarditis(VMC) mice models and to clarify the possible mechanism.Methods The models of VMC were established by injecting Coxsackie virus B3 (CVB3) solution into 50 BALA/C mice.The 31 mice were divided into model group(n =11),large dose BL group(n =10)and small dose BL group (n =10).At the same time the control group (n =10) was established.The mice in model group and control group were fed with water 1 mL/d,the mice in large dose BL group were fed with BL at a dose of 7.5 g/(kg · d),and the mice in small dose BL group were fed with BL at a dose of 2.5 g/(kg · d).After 60 d,the levels of angiotensin Ⅱ (Ang Ⅱ) in blood serum of mice in various groups were detected by using ELISA.Myocardium tissue of mice was stained by Masson and collagen volume fraction(CVF)was accounted.At the same time,the content of periostin mRNA and transforming growth factor β1 (TGF-[β1) mRNA in myocardium tissue of mice were detected by adopting reverse transcription(RT)-PCR.Results Compared with the control group,the levels of serum Ang Ⅱ,CVF,TGF-β1 mRNA and periostin mRNA were increased (all P ＜ 0.01) in the model group.Compared with the model group,the levels of serum Ang Ⅱ,CVF and TGF-β1 mRNA were obviously decreased in large dose BL group and small dose BL group (all P ＜ 0.05).Compared with the model group,the level of periostin mRNA was obviously decreased in large dose BL group (P ＜ 0.05),but the level of periostin mRNA in small dose BL group was not decreased obviously (P ＞ 0.05).Conclusions BL can offer some protection to myocardial fibrosis in VMC mice.The possible mechanism may be performed through inhibiting the expression of serum Ang Ⅱ and TGF-β1 in myocardium tissue to reduce the expression of periostin.

AIM: The effects of selenium dioxide (SeO_2) on proliferation, apoptosis, intracellular reactive oxygen species (ROS) and Ca~(2+) levels in three leukemia cell lines NB4, K562 and HL-60 were investigated. METHODS: Three leukemia cell lines were treated with 3-30 ?mol/L SeO_2. Flow cytometry was used to detect apoptosis rate, and analyze the changes of ROS and Ca~(2+) level within cells. RESULTS: SeO_2 at 10 and 30 ?mol/L inhibited proliferation in three leukemia cell lines. Treatment with 30 ?mol/L SeO_2 for 48 h induced 54.0%, 46.5%, 49.6% apoptosis in NB4, K562, and HL-60 cells, respectively, and also markedly decreased ROS and Ca~(2+) levels among three cell lines. The rate of ROS positive cells in NB4 and HL-60 decreased with the increase in SeO_2 concentrations. ROS was clearly reduced with 30 ?mol/L SeO_2 in K562. Ca~(2+) levels were tardily declined with 10, 30 ?mol/L SeO_2 in NB4 and HL-60 cells. Ca~(2+) levels were clearly reduced with 30 ?mol/L SeO_2 in K562. CONCLUSION: SeO_2 induces apoptosis in three leukemia cells. The declines of intracellular ROS and Ca~(2+) levels are involved in apoptosis induced by SeO_2.