Objective To investigate the the quasispecies character of 3′ untranlated region(3′ UTR) in hepatitis C virus(HCV) by analysing the nucleotide sequence polymorphism and mutation features in 3′ UTR region.Methods Patients infected with genotype 1b HCV were identified by reverse transcription-nested polymerase chain reaction(RT-PCR) and restriction fragment length polymorphism(RFLP) assay.Fragments of the cDNA of 3′ UTR were amplified using semi-nested RT-PCR,and subjected to cloning.The 12-15 clones that contained HCV 3′UTR gene fragments amplified from each patients were sequenced.Results The full-length sequence of 1b genotype HCV 3′UTR in cDNA were obtained.The 3′UTR region consists of four elements: the 5′ region,the poly(U),poly(U/C) and 98-base region.The nucleotide sequence diversity ranged from 0.2%～2.1% and the mutation points were almost distributed in the the 5′region and poly(U/C).Conclusions The HCV has complex quasispecies character in 3′ UTR.

Some cultural practices of a new hybrid yuanhu were reported. The planting deusity of thc new variety wast using small seed tubers (2.8g/Per tuber ), 50~75 tubers/m2 for reprodu ction purpose, 100 tubers/m2 for high yield. 75 tubers/m2 was a suitable density when large or middle seed tubers was used. High reproduction coefficient and yield were obtained when the planting depth was 10cm. It was necessary to irrigate the hybrid yuanhu in North China.Adopting the negative pressure valuc of soil tumidometer as the guide of beginning to irrig ate and to obtain high reproduction cocfficient and to save water, 26.7kPa would be adopted as the initial point of irrigation, excecding 40.0 kPa led to a serious reduction of yield. The difference of yield between rubber tuber irrigaticn and drip irrigation treatment was not obvious, when planting with covering straw in level bed.

BACKGROUND:Total knee replacement is the main treatment for the end of the knee joint disease. How to choose prosthesis replacement according to the different situations of the patient is one of the focuses in the field of joint surgery.
OBJECTIVE: To evaluate middle or short term effects of rotating high-flexion prosthesis for the middle-aged patients with osteoarthritis of knee in total knee replacement.
METHODS: We retrospectively analyzed 48 osteoarthritis patients (55 knees) undergoing total knee replacement with rotating high-flexion prosthesis in the Liuzhou People’s Hospital from January 2009 to January 2013.
RESULTS AND CONCLUSION:(1) Forty-eight patients were folowed up for 23-42 months, mean (24±2.7) months. (2) Knee range of motion and Hospital for Special Surgery knee score were significantly increased after implantation. The excelent and good rate of Hospital for Special Surgery knee score was 85.4%. (3) No infection, loosening or dislocation occurred in al patients. (4) These findings suggest that curative effect of rotating high-flexion prosthesis is definite. After implantation, the range of motion was high, which was accorded with movement pattern of the human body. Prosthesis has a long life, and its repair rate is low. It is an ideal method for the middle-aged patients who want to perform high-flexion activities.

OBJECTIVETo obtain very end full-length cDNA of hepatitis C virus (HCV) 5' untranslated region (5' UTR), and analyse its primary and secondary structure.

METHODSBy reverse transcription-nested polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP), a patient infected with genotype 2a HCV was found. Total RNA isolated from the serum as template, the cDNA of 5' noncoding region was amplified using rapid amplification of cDNA ends methods (RACE), the fragments were recombined by A-T clone strategy, the recombinants were confirmed by RFLP and PCR then sequenced. Secondary structures were analysed by RNA draw.

RESULTSVery end full-length cDNA of 2a genotype HCV 5' UTR was obtained by RACE. In five clones obtained, three contained full-length 5' UTR cDNA, and A21G, G170A, T222C, T247C, C339T substitutions were found compared with HC-J6. he homologies with HCV-1,HC-J6,HC-C2, HC-J8 were 93.6%-94.4%, 92.1%-93.0%, 98.8%-99.7%, 96.2%-96.5%, respectively; however, the substitutions did not alter the secondary structure. Two out of five clones were deleted to have 53 and 144 bases at 5' terminus of HCV 5' UTR, respectively.

CONCLUSIONSRACE is rapid and effective, works well to obtain very end of virus genome. With that, Authors obtained full-length cDNA of genotype 2a of HCV 5' UTR. There are genes deleted at 5' terminus circulated in hepatitis C patients.

5' Untranslated Regions ; genetics ; Base Sequence ; DNA, Complementary ; genetics ; DNA, Viral ; genetics ; Hepacivirus ; classification ; genetics ; isolation & purification ; Hepatitis C ; virology ; Humans ; Molecular Sequence Data ; Nucleic Acid Amplification Techniques ; methods ; Polymorphism, Restriction Fragment Length ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Analysis, DNA

3' Untranslated Regions ; genetics ; Base Sequence ; China ; DNA, Complementary ; chemistry ; genetics ; Genetic Variation ; Hepacivirus ; genetics ; Humans ; Molecular Sequence Data ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Alignment ; Sequence Analysis, DNA ; Sequence Homology, Nucleic Acid

OBJECTIVEThe objective of this paper is to investigate the pharmacokinetics of Chlorogenic acid (CA)and CA-Baicalin compound after intravenous injection (iv) in rabbits.

METHODTen rabbits were randomly divided into two groups. One group were administered CA (6 mg x kg(-1)) by iv, while the other were treated with CA (6 mg x kg(-1))-baicalin (90 mg x kg(-1)) compound by iv. administration. The concentration of CA in plasma was determined by HPLC. The key parameters of pharmacokinetics were calculated and analyzed by kinetic software.

RESULTBoth Concentration-time courses of CA alone and CA-Baicalin compound were consistent with a two-compartment model after administration. The key pharmacokinetic parameters of CA alone were significantly different from that of CA-Baicalin compound (P < 0.05). Compared with the group treated with CA alone, the group treated with CA-Baicalin compound had dramatic increased in AUC(0-infinity), MRT and T1/2alpha.

CONCLUSIONThere are significant difference between the pharmacokinetics of CA and CA-Baicalin compound in rabbits. Baicalin has impact on the pharmacokinetics of CA in rabbits.

Animals ; Calibration ; Chlorogenic Acid ; administration & dosage ; pharmacokinetics ; Flavonoids ; pharmacology ; Injections, Intravenous ; Linear Models ; Male ; Rabbits ; Tissue Distribution ; drug effects

Objective To investigate the effect of scan table on size-specific dose estimate ( size-specific dose estimate, SSDE) in children's CT scan. Methods CT imaging data and CTDIvol of 44 children ( 15 heads, 13 chests, 16 abdomen-pelvis) who underwent Siemens SOMATOM Definition AS+ 64 row 128-slice CT scan were retrospectively collected. CTDIvol of each patient was recored, WED ( water equivalent diameter) was calculated by two different methods ( with or without table) , donated as WED-T and WED-NT, then the corresponding SSDEWED ( SSDEWED-T and SSDEWED-NT ) was calculated. And the SSDEWED-NT was used as reference to evaluate the difference between WED and SSDEWED obtained by two different methods. Results Including part of table will lead to the overestimate for WED, with mean differences of 0. 10％, 2. 82％ and 2. 54％ for head, chest and abdomen-pelvis, respectively, while SSDEWED will be underestimated by 0. 06％ ( head ) , 2. 70％ ( chest ) and 1. 59％ ( abdomen-pelvis ) . Conclusions Including par of the patient table has a certain effect on SSDEWED for children, more attention should be paid for the application of SSDEWED.