Objective To explore the effect of emodin on the apoptisis and intracellular Ca2+ concentration of Hepatic Stellate Cell-T6.Methods The immortalized rat hepatic stellate cell line HSC-T6 was commonly allocated into normal control group and treated groups with different concentrations emodin(the extreme concentration are 10?mol/ml, 20?mol/ml, 40?mol/ml respectively). The cell apoptosis rate were analyzed by flow cytometry, and intracellular Ca2+ concentration of HSC-T6 were detected by Furo-3/Am fluorescence assay and confocal laser microscopy.Results After intervention of different concentration emodin,the apoptosis rate of HSC-T6 is 4.0?0.5%, 4.3?0.6%, 6.9?1.1% respectively,and there are statistical significance compared with the control group(P

Objective To investigate the effects and toxic side effects of concurrent radiotherapy and chemotherapy combined with adjuvant chemotherapy in nasopharyngeal carcinoma.Methods 141 patients of nasopharyngeal carcinoma divided into two groups randomly.All patients treated two-dimension exact radiotherapy and concurrent chemotherapy which combined DDP and 5-Fu intravenously for two circles,then experiment group started adjuvant chemotherapy 3 weeks later after above treatment,which intravenous DDP and 5-Fu for other three circles.Results All patients completed concurrent radiotherapy and chemotherapy,63 patients of treatment group completed adjuvant chemotherapy.The mainly toxic side effect was mucositis,leukopenia and low-platelet,and gastrointestinal reaction during therapy,there is no statistical significance in two groups.The regression rate of lymph nodes is 94.4 ％(n=67)and 87.3 ％(n=62)in nasopharynx and neck after 3 month of radiotherapy and concurrent chemotherapy.All patients followed up 3 years.The total survival rate of two groups were 94.3 ％,84.8 ％,78.6 ％ and 90.1％,75.0 ％,62.5 ％ respectly in 1,2 and 3 year,the mean survival period was 36 months and 27 months,and this differece was statistical significance(x2=7.356,P=0.007).The total survival rate of Ⅲ stage patients of two groups were 98.2 ％,92.5 ％,83.7 ％ and 93.0 ％,83.2 ％,68.2 ％ respectly in 1,2 and 3 year,the both mean survival period was 36 months,and this differece was statistical significance(x2=8.081,P=0.005).The total survival rate of Ⅳ stage patients of two groups were 81.3 ％、53.8 ％、42.9 ％ and 78.6 ％、36.4 ％ 、22.3 ％ respectly in 1,2 and 3 year,the meat survival period were 22 month and 14 month respectly,and this differece was statistical significance(x2=3.903,P=0.048).The progression-free survival rate of two groups were 90.0 ％、76.2 ％、68.8 ％,and 81.7 ％、60.3 ％、34.3 ％ respectly in 1,2 and 3 year,the meat progression-free survival period was 30months and 22months,and this differece was statistical significance as well(x2=13.616,P=0.000).Conclusion The therapy of concurrent radiotherapy and chemotherapy combined with adjuvant chemotherapy was obviously increased total survival rate and progression-free survival rate in nasopharyngeal carcinoma,especially to Ⅲ and Ⅳ stage patient of nasopharyngeal carcinoma.But the long period effects still remains unclear.

Objective To measure the organ weights , blood physiological and biochemical parameters , and immune function of Specific Pathogen Free ( SPF) Rag2 knockout ( KO) mice.Methods Rag2 knockout mice were selected at five and ten weeks , and the organ weights , blood physiological and biochemical parameters were observed .The percentages of CD3+, CD4+, CD8+, CD19+, B220+, NK1.1+, and CD11b+were checked by FCM in Rag2 KO mice at six week of age in terms of its T , B lymphocyte function and NK cell activity .Results Among the same sex group of NOD/SCID mice, the weights of brain, lung, spleen, liver, heart, kidney, and the levels of TBIL, WBC in 10 weeks of age are higher than 5 weeks.At the same age, the weights of heart, kidney, liver, spleen, and the levels of AST, ALP, A/G, GLU, PLT, PCT, WBC, and LYM%in male mice are higher than females.The Rag2 KO mice lacks T cells(0.36 ±0.15)%、CD4+T cells(0.21 ±0.06)%、CD8+T cells(0.23 ±0.07)%、CD19+B cells(0.28 ±0.04)%、B220+B cells(2.03 ±0.42)%).The percentage of NK cells is(24.13 ±3.62)%, and the percentage of granulocytes is (57.20 ±3.85)%.Conclusion The study suggests that the organ weights , blood physiological and biochemical parameters are affected by age and gender in Rag2 KO mice, which main biological characteristics are similar with C 57BL/6J mice.The Rag2 KO mice show the deficiency of T , B cells function .

Objective:To investigate the impacts of hirudin(HRD)on neuronal apoptosis and inflammatory response in rats with cerebral infarction by regulating Ras homolog gene family member A(RhoA)/Rho-associated coiled-coil-forming protein kinase(ROCK)signaling pathway.Methods:SD rats were grouped into Ct group,Model group,low-dose HRD group(HRD-L group,13.33 mg/kg),high-dose HRD group(HRD-H group,26.66 mg/kg),positive control Nimodipine group(NMDP group,40 mg/kg),U-46619(RhoA agonist,0.03 mg/kg)group and HRD-H+U-46619 group(26.66 mg/kg+0.03 mg/kg),with 24 rats per group.Except for the Ct group,rats in other groups constructed the rat model of cerebral infarction by the modified suture method.The rats in the Ct group only exposed the blood vessels without incision and suture insertion.After 1 hour of successful modeling,drug treatment was car-ried out,once a day for 4 weeks.Zea-Longa method was applied to evaluate the neurological function of rats;dry and wet weight meth-od was applied to detect the water content of rat brain tissue;2,3,5-triphenyltetrazolium chloride(TTC)staining was applied to deter-mine the volume of cerebral infarction in rats;TUNEL staining was applied to detect the apoptosis of neurons in the CA1 region of the hippocampus of rats.The levels of TNF-α,IL-1β and IL-6 in rat hippocampus were detected by ELISA;the protein expressions of cleaved-Caspase-3 Cleaved-Caspase-3,Bcl-2-associated X protein(Bax),RhoA,ROCK1 and ROCK2 in rat hippocampus were de-tected by Western blot.Results:Compared with Ct group,the neurological function scores,brain tissue water content,cerebral in-farction volume percentage,neuron apoptosis rate,levels of TNF-α,IL-1β,IL-6,the protein expressions of Cleaved-Caspase-3,Bax,RhoA,ROCK1 and ROCK2 increased in the Model group(P<0.05).Compared with Model group,the neurological function scores,brain tissue water content,cerebral infarction volume percentage,neuron apoptosis rate,levels of TNF-α,IL-1β,IL-6,the protein expressions of Cleaved-Caspase-3,Bax,RhoA,ROCK1 and ROCK2 decreased in HRD-L group,HRD-H group,NMDP group,however,the corresponding index changes in the U-46619 group showed an opposite trend(P<0.05).Compared with HRD-H group,the neurological function scores,brain tissue water content,cerebral infarction volume percentage,neuron apoptosis rate,levels of TNF-α,IL-1β,IL-6,the protein expressions of Cleaved-Caspase-3,Bax,RhoA,ROCK1 and ROCK2 were increased in the HRD-H+U-46619 group(P<0.05).Conclusion:HRD may inhibit neuronal apoptosis and inflammatory response in rats with cerebral infarction by inhibiting RhoA/ROCK signaling pathway.

Objective:To investigate the clinical efficacy and safety of domestic bortezomib in the treatment of patients with multiple myeloma (MM).Methods:The data of 60 MM patients treated with domestic bortezomib as the basic chemotherapy regimen in 5 medical centers of Qilu Hospital of Shandong University, Heze Municipal Hospital, Weifang People's Hospital, Binzhou Medical University Hospital, Zibo Central Hospital in Shandong Province from January 2018 to June 2019 were retrospectively analyzed, 52 of which were newly treated patients and 8 were relapsed and refractory patients. The patients received at least 2 courses of combined chemotherapy based on domestic bortezomib, and the efficacy was assessed and evaluated every 2 courses.Results:Follow-up until June 30, 2019 showed that some patients were unable to return to the hospital for regular treatment. All patients completed at least 2 courses of treatment, with an overall effective rate (ORR) of 76.7% (46/60); 42 patients completed 4 courses of treatment, with an ORR of 78.6% (33/42); 30 patients completed 6 courses of treatment, with an ORR of 86.7% (26/30); there was no significant difference in ORR of 2, 4 and 6 courses ( P > 0.05). The complete remission+very good partial remission rates of 2, 4 and 6 courses were 16.7% (10/60), 47.6% (20/42) and 66.7% (20/30), respectively, and the difference was statistically significant ( P < 0.01). During the treatment, the adverse events mainly included infection, peripheral neuropathy, herpes, digestive tract symptoms, hematologic toxicities and so on, which were light and moderate mostly, and most of them can be reversed. The total incidence of adverse events in patients who completed 2, 4 and 6 courses of treatment were 91.7% (55/60), 66.7% (28/42) and 36.7% (11/30), respectively. Conclusions:The domestic bortezomib-based chemotherapy regimens have good efficacy in the treatment of MM. The incidence of adverse events is similar to that of the original drug, and patients can tolerate the adverse events.