Introduction: One of the world’s leading causes of mortality, WHO projects that diabetes will be the seventh leading cause of death in 2030. Therefore, there is absolutely need for prevention and treatment of diabetes, and scientists are keen to introduce a variety of drug and non-drug treatment methods. Goal: To examine effect of Artemisia commutata Bess aqueous extract on Wistar rats with diabetes model type II induced by STZ with high fat diet. Material and Methods: Experimental was performed in Institute of Mongolian medicine and Chemistry, Inner Mongolia University. In study, 32 Wistar rats (body weight 180-200 g, healthy) were divided into 4 groups included Normal, Model, Metformin and Artemisia commutata Bess. Type II diabetes were induced by intraperitoneal single dose STZ 56.25 mg/kg with high fat diet except for Normal group. Then Metformin group was received by oral administration at a dosage of 50 mg/kg/day and Artemisia commutata Bess group was received by oral administration at a dosage of 55 mg/kg/day during 30 days. Blood glucose (GLU), triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), aspartate aminotransferase (ASAT) and alanine aminotransferase (ALAT) levels were tested at the end of the experiment. Pancreatic sections were stained with hematoxylin-eosin (HE). Results: The level of blood glucose was significantly increased in Model group (23.9±1.33 mmo/L) compare to Normal group (5.64±0.24 mmol/L). Oral amdinistration of Artemisia commutata Bess 55 mg/kg/ day to treated group (9.75±1.84 mmol/L), Metformin 50 mg/kg/day to treated group (9.04±2.75 mmol/L) resulted in significantly decreased of blood glucose level less Model group. Conclusion This study demonstrated that hypoglycemic effect of Artemisia commutata Bess on diabetes model type II induced by STZ with high fat diet in Wistar rats.