This paper introduces the design of automatic biochemistry analyzer based on CAN field bus. The hardware and software of a single network node and several problems are narrated. Some related flow charts and source codes are also given. The involvement of CAN bus in the design of automatic biochemistry analyzer enhances the efficiency and the degree of automatization.

Objective: To investigate the effects of fracture liaison service (FLS) model on the medication compliance of patients, quality of life, pain and re-fracture rate in patients with osteoporotic vertebral compression fracture (OVCF). Methods: A prospective case control study was conducted to analyze the clinical data of 117 OVCF patients admitted to Department of Pain of Guizhou Provincial People's Hospital from January 2017 to September 2017. According to the random table method, patients were divided into control group (58 patients) and intervention group (59 patients). Routine nursing was adopted in the control group, and fracture liaison service mode was adopted in the intervention group on the basis of the control group until 3 months after discharge. Morisky scale was used to compare the medication compliance of patients on admission, at discharge, 1 month, and 3 months after discharge, and SF-36 was used to evaluate the quality of life. The SF-36 scale contained eight dimensions including physiological function, physiological performance, physical pain, overall health, vitality, social function, emotional function and mental health. Visual analogue scale (VAS) was used to evaluate the pain of patients. The re fracture rates of the two groups were compared. Results: There were no significant differences between the two groups concerning gender, age, history of diabetes, history of hormone use, number of vertebral fractures, fracture site and cause of injury (P>0.05). In the intervention group, the medication compliance scores of patients in the first and third months after discharge were (5.5±2.0)points and (6.3±1.8)points, which were higher than those in the control group [(3.5±2.2)points and (3.3±1.8)points] (P<0.05). Within each group, there were significant differences among the medication compliance scores at the different time points of evaluation (on admission, at discharge, 1 month, and 3 months after discharge). The scores of the eight dimensions of the SF-36 scale in the intervention group were higher than those in the control group at 1st and 3rd months after discharge (P<0.05). Within each group, there were significant differences among the SF-36 scores at the different time points of evaluation. The pain scores of the intervention group at discharge and 1st and 3rd months after discharge were ( 3.1±1.7)points, (1.8±1.3)points, (1.4±1.3)points, all lower than those in the control group [(5.2±1.7)points, (3.6±1.6)points, (2.7±1.8)points] (P<0.05). Within each group, there were significant differences among the pain scores at the different time points of evaluation. At 1st and 3rd months after discharge, the re-fracture rates of the intervention group were both 2%, and the those of the control group were 3% and 9%, respectively, showing no significant difference between the two groups (P>0.05). Conclusion The FLS model can effectively alleviate the pain of OVCF patients, improve the medication compliance and quality of life of patients after discharge, but it cannot reduce the short-term re fracture rate after discharge.

Objective: To evaluate the effectiveness of eradication therapy based on Helicobacter pylori (Hp) susceptibility and CYP2C19 genotype in children with refractory Hp infection. Methods: In this prospective observational cohort study, 156 children with Hp refractory to amoxicillin+clarithromycin+omeprazole triple regimen in Baoding Children′s Hospital from December 2017 to May 2018 were enrolled. Ninety-two of them underwent Hp culture and CYP2C19 detection. Seventy-five cases with positive Hp culture were defined as culture successful group and were treated according to Hp susceptibility and CYP2C19 genotype. Seventeen cases with negative Hp culture were defined as culture failed group and were treated only based on the results of CYP2C19 genotype. Sixty-four children who did not have Hp culture and CYP2C19 gene testing were defined as the empirical eradication therapy group and were treated with quadruple regimen (amoxicillin+metronidazole+omeprazole+bismuth). Bacterial resistance, CYP2C19 polymorphism and therapeutic effectiveness between the three groups were compared using chi-square test. Results: Among the 75 positive Hp culture results, 72 (96%) were resistant to clarithromycin, 3 (4%) were resistant to metronidazole, 5 (7%) were resistant to levofloxacin, 5 (7%) were resistant to rifampicin, 1 (1%) was resistant to tetracycline, and none was resistant to amoxicillin and furazolidone. The CYP2C19 polymorphism in 92 patients showed that 43 (47%) were extensive metabolizer (EM), 9 (10%) were poor metabolizer (PM), and 40 (43%) were intermediate metabolizer (IM). In terms of the effectiveness, eradication rate in the culture successful group,culture failed group and empirical eradication therapy group were 99% (74/75), 88% (15/17) and 72% (46/64), respectively (χ²=21.325, P<0.05). The eradication rate in the culture successful group was significantly higher than that in empirical eradication therapy group (χ²=21.005, P<0.05), while there was no difference between empirical eradication therapy group and culture failed group (χ²=1.154, P=0.283). Conclusion Eradication regimen based on bacterial susceptibility and CYP2C19 genotype should be considered in children with refractory Hp infection.