Objective To observe the change of the concentration of plasma B-type natriuretic peptide (BNP) before and after treadmill exercise test (TET),and to judge the accuracy of BNP changes ( ABNP)in diagnosing coronary heart disease (CHD). Methods A total of 188 patients with suspected CHD underwent TET and the levels of plasma BNP before and after TET was detected. All the patients were divided into CHD group( group A, n = 96) and non-CHD group(group B, n = 92) according to coronary ar-teriography. Meanwhile, 26 healthy volunteers were enrolled (group C). Results The levels of plasma BNP after TET increased significantly (P<0.01 ). The levels of ABNP of group A were obviously higher than those of group B and C(P<0.01 ). When the levels of △BNP was 8.5 pg/ml, the sensibility, specificity, pos-itive predictive value and negative predictive value of diagnosing CHD was 93.5%, 57.1%, 70.5% and 88.9% respectively. Area under curve (AUC) was 0.815, and 95% confidence interval was 0.768-0.896.Conclusions The levels of plasma BNP after TET increase obviously. It exists higher sensibility and speci-ficity in diagnosing CHD by △ABNP set 8.5 pg/ml.

ObjectiveTo explore the effect of age on clinical outcomes of patients with massive infarction after decompressive craniectomy.MethodsClinical data of 103-sufferers,who have been operated by decompressive craniectomy in our hospital from January 1999 to January 2010 were summarized.Based on age,these data are divided into the research group( ≥60 years old,number =48) and the control group( ＜ 60 years old,number =55 ).Then two groups were studied and discussed by mortality,the ratio of postoperative complications and cases of preoperative comorbidities.ResultsComparing with the control group,it was evident that patients of the research group had higher mortality and ratio of postoperative complications,and moat of them suffered from preoperative manifold comorbidities ( x2 =11.097,24.915,14.404,all P ＜ 0.01 ).Besides,the percentage of patients with good prognosis was significantly lower( x2 =9.821,P ＜ 0.01 ).ConclusionPostoperative clinical outcomes of aged patients with the massive infarction were affected by preoperative manifold comorbidities and postoperative complications.However,younger patients could correspondingly achieve the preferable treatment effect after surgery.Hence,age should possess an imporrant impact for clinical outcomes of patients with massive cerebral infarction after decompressive craniectomy.

Objective:To investigate the syndromes distribution in patients with dysfunctional uterine bleeding(DUB) related to kidney deficiency, and to provide the basis for standardizing the DUB syndrome differentiation of kidney deficiency. Methods: DUB Patients with kidney deficiency were chosen and accepted epidemical survey through the questionnaire, then the frequency and constituent ratio of each syndrome were calculated. Results: In 612 cases of DUB patients with kidney deficiency, there were 102 patients with simple syndromes covered 16.67%, and 510 patients with compound syndromes covered 83.33%. In patients with simple syndromes, the common syndromes were kidney-yin deficiency(45,44.12%) and kidney-qi deficiency(45,41.18%). And the syndromes of kidney-qi deficiency and kidney-yin deficiency were often respctively complicated with liver stagnation(68,11.09%; 113,18.43%), blood defi ciency(57,9.30%; 99,16.15%) and blood stasis(57,9.30%; 99,16.15%), the syndrome of kidney-yang deficiency usually complicated with spleen-qi deficiency(75,12.23%). The syndrome of kidney-qi deficiency(186,57.23%) was commonly-seen in the patients with ovular DUB, while the syndromes of kidney-qi(146,50.69%) and kidney-yin deficiency(111,38.54%) were frequently-seen in the patients with anovular DUB. Conclusion: In clinic, the compound syndromes were commonly seen in patients with DUB due to kidney deficiency, the complicated syndromes of kidney deficiency were liver stagnation, spleen-qi deficiency, blood deficiency and blood stasis..

OBJECTIVETo evaluate its mechanism of inducing the maternal-fetal immune tolerance by studying the effects of Shoutai pills on the expression of Th1/Th2 cytokine and pregnancy in maternal-fetal interface of mice with recurrent spontaneous abortion (RSA).

METHODThe normal pregnancy and RSA model were respectively induced with CBA/J x BALB/c and CBA/J x DBA/2. The mice with RSA were randomly divided into model group and low, middle and high dose groups of Shoutai pills. The mice were killed in 14 days after administration and embryo resorption rate was counted and their decidual and placental tissues were co-cultured to detect the expressions of IL-4, IL-10, IFN-gamma and TNF-alpha with ELISA.

RESULTThe embryo resorption rate of the model group was significantly higher than the normal pregnancy, middle and high dose groups of Shoutai pills could decreased the embryo resorption rate of the mice with RSA (P < 0.05). All the doses in 3 groups of Shoutai pills could decreased the expression of IFN-gamma and TNF-alpha (P < 0. 05) and there was no obvious difference between normal pregnancy group and all groups of Shoutai pills. Middle and high doses of Shoutai pills could increased the expression of IL-4 and IL-10 (P < 0.05) and there was no obvious differences between normal pregnancy and high dose group of Shoutai pills.

CONCLUSIONThe mechanism about Shoutai pills can change Th1 /Th2 cytokine towards Th2 bias, which induced the maternal-fetal immune tolerance.

Abortion, Habitual ; drug therapy ; immunology ; pathology ; Animals ; Cytokines ; immunology ; Drugs, Chinese Herbal ; administration & dosage ; Embryo Loss ; Female ; Humans ; Male ; Maternal-Fetal Exchange ; drug effects ; Mice ; Mice, Inbred BALB C ; Mice, Inbred CBA ; Mice, Inbred DBA ; Pregnancy ; Pregnancy Outcome ; Th1 Cells ; drug effects ; immunology ; Th2 Cells ; drug effects ; immunology

Exposure to particulate matter 2.5 (PM2.5) potentially triggers airway inflammation by activating nuclear factor-κB (NF-κB). Sirtuin 2 (SIRT2) is a key modulator in inflammation. However, the function and specific mechanisms of SIRT2 in PM2.5-induced airway inflammation are largely understudied. Therefore, this work investigated the mechanisms of SIRT2 in regulating the phosphorylation and acetylation of p65 influenced by PM2.5-induced airway inflammation and bronchial hyperresponsiveness. Results revealed that PM2.5 exposure lowered the expression and activity of SIRT2 in bronchial tissues. Subsequently, SIRT2 impairment promoted the phosphorylation and acetylation of p65 and activated the NF-κB signaling pathway. The activation of p65 triggered airway inflammation, increment of mucus secretion by goblet cells, and acceleration of tracheal stenosis. Meanwhile, p65 phosphorylation and acetylation, airway inflammation, and bronchial hyperresponsiveness were deteriorated in SIRT2 knockout mice exposed to PM2.5. Triptolide (a specific p65 inhibitor) reversed p65 activation and ameliorated PM2.5-induced airway inflammation and bronchial hyperresponsiveness. Our findings provide novel insights into the molecular mechanisms underlying the toxicity of PM2.5 exposure. Triptolide inhibition of p65 phosphorylation and acetylation could be an effective therapeutic approach in averting PM2.5-induced airway inflammation and bronchial hyperresponsiveness.
Animals ; Inflammation ; Mice ; NF-kappa B/metabolism* ; Particulate Matter/toxicity* ; Signal Transduction ; Sirtuin 2/metabolism* ; Transcription Factor RelA/metabolism*