Objective To identify the effect of decreasing the rejection using the biological semipermeable membrane combined with the privileged site xenotransplantation. Methods The rat ICCs encapsulated by biological semipermeable membrane were xenotransplanted into dog's brain. The pathological changes of implants and surrounding cerebral tissues were observed under light and electric microscopy. The ?-cells of implants were identified by immunohistochemistry. Results After 2 months of transplantation, The ?-cells were observed and the lymphocytes were dispersed in the grafts.The brain tissues near the grafts showed slight edema and glial hyperplasia. Conclusion The method using the biological semipermeable membrane in combination with the privileged site xenotransplantation have a beneficial effect on the inhibition of the rejection of heterogeneous ICCs implant.

Objective To study the mechanism of arachnoidal fibrosis after subarachnoid hemor- rhage. Methods Rats were divided into control group, experiment group and treatment group. Radioim- munoassay (RIA) was employed to detect the levels of hyaluronic acid, laminin,type Ⅲ precollagen and type Ⅳ collagen in the arachnoid membrane. In the meantime, arachnoid cell's morphology and collagen distribution in the subarachnoid space were investigated by electron microscope. Results Results of RIA detection showed increase of Type Ⅲ precollagen level (peak at the second week), obvious higher levels of LN and HA but insignificant change of type IV collagen after subarachnoid hemorrhage. However, dexam- ethasone treatment decreased type Ⅲ precollagen level. Electron microscope found that arachnoid cells pres- ented accentruated bioactivity after subarachnoid hemorrhage, with significant increase of arachnoidal colla- gen fibers from one week after suharachnoid hemorrhage, continuing for 3 weeks. Dexamethasone treatment resulted in low density of mitochondria and sparsed arachnoidal collagen fibers. Conclusions Extracellu- lar matrix (ECM) increases in arachnoid membrane after subarachnoid hemorrhage and participates in a- rachnoid fibrosis. Dexamethasoue can relieve arachnoidal fibrosis after subarachnoid hemorrhage, as pro- rides fresh way for prevention and treatment of post hemorrhagic hydrocephalus.

Objective To learn about the status and demand for geriatric nursing talents in nursing home of Zhejiang province , and to explore the evidence for cultivating geriatric nurse in nursing home . Methods A total of 253 nursing homes of Zhejiang province were selected and surveyed using a self -designed questionnaire .Results Nursing homes in Zhejiang province did not have enough geriatric nurses , and the nurses in the nursing homes were with low education , within which the nurses with technical secondary school qualification accounted for 62.4%, and the nurses with college degree accounted for 28.5%.All nursing homes needed geriatric nurse with different education background .Long term care and other 12 fields’ knowledge were demanded .Health education and other 10 kinds of core ability were required .More than 90% respondents believed that it was necessary to set the major of geriatric nursing .Conclusions Nursing homes are lack of nursing talents , which are in great need of geriatric nurses with different education backgrounds .Based on the demand for knowledge and core competence , we should cultivate geriatric nursing talents through changing the courses system, the way of practice and setting the major of geriatric nursing to meet the needs for the aging of population in China .

Objective To study the status and need for geriatric nursing talents in community , so as to explore the evidence for cultivating community geriatric nurse .Methods Totals of 284 community health service centers of Zhejiang province were surveyed with a self -designed questionnaire .Results Among 284 community health service centers , 72.5 percent investigators believed that the number of geriatric nurse in community can not met the need of elderly care , 67 .6 percent investigators believed that the professional quality of geriatric nurse can not met the need of elderly care .Geriatric nurses with college degree account for 44.5 percent of all the community nurses, technical secondary school level account for 39.8 percent, 61.2 percent of the nurses recruited in future years are demanded with college degree .Geriatric nursing knowledge and skill and other 11 courses should be designed to cultivate community geriatric nurse , the ability of care for elderly with common disease and drug reaction and other 8 kinds of abilities are the core ability of geriatric nurse in community . Conclusions Both the number and quality of geriatric nurse can not meet the need of elderly care now .Guiding by elderly care knowledge and core abilities , we should cultivate community geriatric nurses through setting the major of geriatric nursing , changing the courses system , the way of teaching and practice to meet the needs for the aging of population in China .

The incidence of intracranial aneurysms is high, which is the first cause of spontaneous subarachnoid hemorrhage. The preparation of animal models for intracranial aneurysms is becoming increasingly mature, and has played an important role in research fields of etiology and intervention materials for intracranial aneurysms. This article reviews preparation methods and animal selection of animal model for intracranial aneurysms.