Objective To investigate the value of expressed prostatic secretion (EPS) leukocyte count in the diagnosis of chronic prostatitis. Methods EPS was obtained from 75 out patients with chronic prostatitis,pH and WBC per high power microscopic field being studied.Transabdominal prostatic ultrasonography was performed. Results Mean pH of the EPS was 6.9?0.4.pH of the EPS and the frequency of ultrasound abnomalities were significanly correlated to the leukocyte count in EPS.There was no correlation between age of symptom duration and the pH of EPS, frequency of ultrasound abnormalities or white blood cell counts in EPS. Conclusions In chronic prostatitis,the leukocyte count in EPS was positively correlated with the ultrasound abnomalities and the pH elevation of EPS.There was no correlation between age or symptom duration and pH of the EPS,frequency of ultrasound abnormalities or leukocyte count in the EPS.

Objective:To study the expression of NF-?Bp65 and bcl-x L gene in pancreatic carcinoma and their clinical significance.Methods:The expressions of NF-?Bp65 and bcl-x L protein in 52 pancreatic carcinoma were studied by immunohistochemistry,and their relationship to clinical pathology were analyzed.Results:The positive rates of NF-?Bp65 protein and bcl-x L protein in pancreatic carcinoma were 63.5% and 73.1% respectively,and which were higher than those of normal pancreatic tissues.The expression of NF-?Bp65 was related to tumor diameter,TNM stage and lymph node metastasis,and had no relation with the extent of differentiation and type of pathology.There was positive correlation between NF-? Bp65 and bcl-x L expression.Conclusion:NF-? Bp65 may be an important oncoprotein,and may have positive regulation on bcl-x L,then promote tumorgenesis.

A high sensitive and selective imprinted electrochemical sensor based on cobalt nanoparticles/gra-phene modified carbon electrode was prepared by electropolymerization technqiue with p-tert-octylphenol as the template and pyrrole as the functional monomer. The electrochemical behaviors of the imprinted sensor were evaluated with cyclic voltammetry and differential pulse voltammetry. The response currents of the imprinted electrochemical sensor exhibited a linear relationship toward the negative logarithm of p-tert-octylphenol concentrations ranged from 1. 0í10-13 mol/L to 1. 0í10-7 mol/L (R2=0. 994) with the detection limit of 3 . 7í10-14 mol/L ( S/N=3 ) . The imprinted electrochemical sensor was respectively applied to the determina-tion of p-tert-octylphenol in rain water, lake water and drink with the recoveries of 92. 4%-102. 0%.

2 67kPa) after MVR were enrolled in the study Parameters in hemodynamics and oxygenation were measured with Swan Ganz technique , 5min before NO inhalation (T 1), 5,15 and 30min following NO 20ppm inhalation (T 2,T 3,T 4), 15min after stopping NO 20ppm inhalation (T 5), 15min and 5h following NO 6ppm inhalation (T 6,T 7) and 15min before and after weaning from mechanical ventilator (T 8,T 9) respectively Results Compared with those at T 1, at T 2 pulmonary artery pressure, pulmonary vascular resistance, alveolar arterial oxygen difference and intrapulmonary shunting significantly decreased, but arterial oxygen partial pressure, oxygen content and oxygen availability index increased significantly (P0 05) Conclusions The persistently inhaled low dose NO is effective and safe for the pulmonary hypertension induced with heart surgery

[Objective] To investigate the influence of Chitosan fiber combined with gelatin on the mechanical property of calcium phosphate cement.[Methods]Calcium phosphate cement composite by the incorporation of chitosan fiber and 5 wt% gelatin was investigated on flexural strength,phase composition,scanning electron microscopy observation and cytotoxicity assays.[Results]Fiber volume fraction had a significant effect on the flexural strength,and the post hoc test revealed that significant difference in the flexural strength was recognized(P

To investigate the protective effects of OLA-PENa on experimental hepatic injury in rats and compare it with OLA in the dose-response relationships. METHODS: Biochemical indexes and histopathological examination of hepatic injury in rats caused by toxicant chemicals ［D-galactosamine (D-Galn) and CCl4］ were determined. RESULTS: OLA-PENa obviously inhibited the rising of serum alanine transaminase (ALT) caused by D-Galn and CCl4 and dramatically decreased liver fat storage as well in a dose-dependent manner. Histopathological examination showed that OLA-PENa can evidently alleviate the condition of the degeneration of hepatic cells and that of necrosis. CONCLUSION: The protective effects of OLA-PENa on experimental liver injury in rats, all the mentioned effects, are more powerful than those of OLA with the same dosage.

Objective To study the influence of finasteride on the expression of hypoxia-inducible factor 1 α(HIF-1 α) and vascular endothelial growth factor (VEGF) with prostate cancer (Pca).Methods The patients diagnosed of Pca were selected,and which treated with finasteride for up to six months were finasteride group and untreated with finasteride were control group.The expression of HIF-1 α,VEGF and microvessel density(MVD) was examined.Results The positive expression of HIF-1α,VEGF and MVD in the finasteride group were 55.0％,57.5％,(56.9 ± 13.8),respectively,and were lower than those of the control group [87.5 ％,97.5 ％,(64.2 ± 13.3)] (x2 =10.313,18.122,all P ＜ 0.01 ; t =2.391,P ＜ 0.05).In the pathological grade,advanced grade of the finasteride group was in 15 cases(37.5％),and 10 cases(25.0％) was in the control group,and the difference was statistically significant between the two groups(x2 =1.455,P ＞ 0.05).Conclusion Long-term treatment with finasteride can reduce expression of HIF-1α and VEGF with Pca,thus inhibit angiogenesis and tumor growth and metastasis.

Objective To evaluate free or pedicled perforator flaps for repairing chronic osteemylitis with soft-tissue defect in the distal lower extremity. Methods From May of 2006 to October of 2007, 28 consecutive patients of chronic osteomylitis with soft-tissue defect in the distal lower extremity underwent surgical debridement and reconstruction with free or pedicled perforator flaps. There were 13 free flaps. The free anterolateral thigh flaps were used in 2 cases to repair the soft defects in the front of leg, 3 cases in the front of the malleolus, 2 cases in the dorsum of foot, 2 cases in the heel. The free lateral crural flaps nourished by perone al artery were used in 4 cases to repair the soft defects in the dorsum of foot. There were 15 pedicled flaps. Posterior tibial artery perforator flaps were used in 4 cases to repair the soft defects in the front of leg, and 2 cases in the medial malleolus. Lateral retromalleolar perforator flaps nourished by peroneal artery were used in 6 cases to repair the soft-defects in the heel, 1 case in the lateral malleolus and 1 case in the dorsum of foot, the first dorsal metatarsal artery perforator flap was used to repair the proximal dorsum of hallux. The wound was closed with irrigation-suction in 7 cases and with vancomycin-impregnated gelatin in 8 cases. Results All 27 flaps were successfully survived except insuffcient vein refluence in 1 posterior tibial artery perforator flap, which resulted in a superficial necrosis and healed spontaneously. The follow-up period from 6 months to 2 years revealed that recurrence developed in two diffuse type patients and both were treated once and twice with success, respectively. The others healed without any signs of recurrences. No debulking procedure was necessary in any case. Secondary bone graft was performed in 3 cases. All patients were ambulatory and fully weight-bearing with normal clinical parameters at the time of last review. According to the evaluating criteria for the treatment of foot disease, the mean score was 84.5. Conclusion Free or pedicled perforator flap has been shown to be well vascularised, and it is feasible for the treatment of chronic osteomyelitis with soft-tissue defect in the distal lower extremity.

Objective To study the expression of nuclear factor-?Bp65 (NF-?Bp65) in gastric carcinoma and its relationship with vascular endothelial growth factor (VEGF). Methods The expression of NF-?Bp65 and VEGF in 56 gastric carcinomas was detected with immunohistochemistry and compared with benign tissues. Results The positive rates of NF-?Bp65 and VEGF in 56 gastric carcinomas were 62.5% and 76.8% respectively,and which were higher than those of gastric mucosal atypical hyperplasia (33.3% and 44.4%) and the normal gastric mucosa(0 and 8.3%) (P0.05). There was positive correlation between NF-?Bp65 and VEGF expression (r=0.36,P

OBJECTIVE:To establish an assay method for Sustained-release palmatine tablet in beagle dog. METHODS:Sample separation was performed on Diamonsil ODS C18 column(250 mm?4.6 mm,5 ?m) with mobile phase consisted of acetonitrile-water (1∶1,containing NaH2PO4 3.4 g and SDS 1.7 g in every 1 000 mL water,pH was adjusted to 3 by H3PO4) with a flow rate of 1.0 mL?min-1.The column was kept at 35 ℃ and the detective wavelength was set at 342 nm.RESULTS:The linear serum concentration range was 0.005～0.5 ?g?mL-1(r=0.999 9) and the recovery rate was(102.22?2.48)%. CONCLUSION:The method was proved to be simple,accurate,sensitive and reproducible,and it was up to the standards for the study of pharmacokinetics and bioavailability of Sustained-release palmatine tablet in beagle dog.