OBJECTIVE To summarize and analyze of various consequences caused by clinical vacuum blood sampling. METHODS The data taken from 4000 cases after with vacuum blood sampling were analyzed. RESULTS The unqualified rate of vacuum blood sampling apparatus was 0.1%. There were 12 defect blood sampling cases, and there was no puncture hurt, and infection. CONCLUSIONS Internationally standardized vacuum blood sampling is safe and reliable. A strict guideline applied in vacuum blood sampling is able to control medical cross infections.

Two famous historical events which lead to the establishment of the medical ethics committee are presented in this article. We discuss the significance of the medical ethics committee and put forward 5 propositions to enhance the developing of the committee. Firstly, overcome the scruple and accelerate the construction of the medical ethics committee. Secondly, seek for the ethics spirit from the traditional culture, and establish the principles and the conception correspondingly. Thirdly, be creative and try to adapt to the promotion of medicine and society. Fourthly, strengthen the training of bioethics. Finally, promote the communication between ethics review committees.

To observe the therapeutic effect of Naohuandan Capsule (mainly composed of Radix Ginseng, Radix Rehmanniae Preparata,etc.) in treating mild and moderate cases of vascular dementia (VD).Forty five cases of VD, which are subjected to the diagnostic standard of American Association of Psychiatry (DSM Ⅳ), were randomly allocated to two groups for a 3-month treatment course. Group A (n=22) was treated with Naohuandan Capsule and Group B (n=23) with Naofukang Tablet. Therapeutic effect was evaluated by scoring of mini-mental state examination (MMSE) and activity of daily living (ADL) and serum levels of estradiol (E 2) and testosterone (T) were also observed.Scores of MMSE and ADL were increased, E 2 and T levels were elevated and the ratio of E 2/T was decreased in Group A(P0 05). [Conclusion]Naohuandan Capsule has a definite effect in preventing and treating VD and its mechanism may be related to the increase of E 2 and T levels, decrease of E 2/T ratio and the regulation of neuroendocrine function.

DNA methylation is one of the most common epigenetic events in eukaryotic cell.Aberrant DNA methylation is tightly related to tumorigenesis.Recent evidence indicates that DNA methylation is a promosing biomarker for cancer.The cancer-associated DNA methylation events could be detected from serum(plasma),urine,stool and bronchoalveolar lavage,providing a new choice for noninvasive cancer diagnosis.In this review,we summarized the advances on DNA methylation for noninvasive cancer diagnosis.

Objective To determine and compare the complete sequence of COII gene from Anopheles sinensis, Aedes albopictus and Culex quinquefasciatus and to identify the molecular homology among these mosquitoes. Methods The recombinant plasmids including COII gene from the three species of mosquitoes were obtained by the T A cloning technique,and the COII genes were sequenced and analyzed. Results The homology of the COII gene nucleotide sequence and the deduced amino acid sequence of COII among the three species of mosquitoes varied from 84.1% to 87.9% and from 85.1% to 89.5%, respectively. The C+G content of COII gene was 23.2%-24.9%, the frequency of transversion higher than that of transition. Conclusion Aedes albopictus and Culex quinquefasciatus are closely related species at their COII molecular level according to COII gene.

Objective To study the characteristics of peripheral nerve conduction (PNC) in advanced-stage amyotrophic lateral sclerosis (ALS) patients undergoing mechanical ventilation.Methods The sensory conduction velocity (SCV),motor conduction velocity(MCV),distal motor latency(DML) and amplitude of compound muscle action potential(CMAP) were determined in three ALS patients undergoing mechanical ventilation.The clinical data and PNC characteristics of the patients were analyzed.Results The 12 motor nerves were abnormal in all the three patients,including absence of responsive wave (66.7%,8/12),decreased amplitude of CMAP(33.3%,4 /12),prolongation of DML(16.7%,2 /12) and reduction of MCV(16.7%,2 /12).The CMAP amplitude of patients gradually decreased with the progression of disease,and finally led to adverse reactions.The SCV and amplitude of sensory nerve action potential were normal in all the three patients.Conclusion ALS patients undergoing mechanical ventilation may have severely damaged motor nerve conduction,and their sensory nerve is generally not affected,with the specific reason remains to be further studied.

Objective:To study the relationship between an interpersonal climate of universities and teachers' mental health by self-made Scale of Interpersonal Climate (SIC).Methods:532 teachers from 16 universities all over the country were randomly chosen and assessed with SRHMS and SIC.Results: ①The better interpersonal climates of universities covered around 68.8% ; the most harmonious interpersonal relationships covered 72.4% ; worse interpersonal attitude, worse interpersonal interaction and worse interpersonal distance covered 44.5%,44.5% and 46.1% respectively ; the general feelings towards mental health of teachers in universities were better (the bad self-assessment、the medium self-assessment and better self-assessment are 9.0%,56.1% and 34.9% respectively ) . ② There was prominent positive correlation between universities' interpersonal climate and teachers' mental health(r=0.44). Meanwhile there was prominent negative correlation between negative emotion and interpersonal interaction, interpersonal harmony and interpersonal attitude(r=-0.25,-0.30,-0.30), but there was prominent positive correlation between negative emotion and interpersonal distance(r=0.24). ③ There was very distinct regression between interpersonal interaction and mental health, interpersonal harmony and mental health(?=0.28、0.22).Conclusions: The general feelings towards mental health of teachers in universities are better;There are close relationships between interpersonal climates of universities and teachers' mental health, and interpersonal interaction and interpersonal harmony play a brilliant role in mental health regression , they are the positive predictable factors to teachers' mental health.

Objective To investigate clinical features of alopecia areata such as sex, age, and subjective symptoms, and relationships between these factors and disease severity. Methods Clinical materials of patients who were diagnosed as alopecia areata were collected. The role of sex was analyzed in the disease and the correlation between disease severity and sex, age or psychological factors was analyzed. Results 118 patients including 101 cases of localized alopecia areata (AA), 5 alpecia totalis (AT) and 12 alopecia universalis (AU) were collected. The ratio of male to female was 1.2:1 and there was no correlation between sex and disease severity. The patients were from 1 to 76 years old while average age was 31.3?1.3 years, and that of the female patients was 28.9?1.9 and the male was 33.2?1.8; The median course was 2.5 months, while AA was 2 months, and AT/AU was 18 months; 14.4% patients (17/118), including 11 cases of AA and 6 AT/AU had subjective symptoms; 20.3% patients (24/118) had psychological factors before onset, 60.2% patients (71/118) had difficulties in sleeping, nail changes were found in 15.2% patients (18/118), and ocular symptoms were found in 5.9% patients (7/118), 5.9% patients (7/118) had family history, 3.4% patients (4/118) were accompanied by disease of thyroid gland, 1.7% patients (2/118) were accompanied by vitiligo, TGA and TMA levels elevated abnormally in 4.8% patients (3/62). Conclusion Alopecia areata often affects young to middle-aged people, and there is no correlation between sex and disease severity; Female patients are younger than male; A few patients are induced by psychological factors, and some accompanied by other autoimmune diseases. AT/AU is easier to have subjective symptoms than AA.

Objective The objective of digitalized patient records control is to meet some new demands set on patient records control under the new circumstances. Methods A computer system for digitalized patient records control and supporting processing, management and applications setups were established. Results The system put the contents of the patient records into various categories with difierent standards and designed relatively strict means of authorization. It rendered patient records easier to use and their control more strict, making it possible to make general use of the records without access to the original documents. As a result, data loss was avoided, authority over the data was put under control, data sharing was provided, and full and convenient use of data was realized. Conclusion Digitalized patient records control, a form of shaping computer-based patient records before the latter fully comes into being, enhances the efficiency of patient records application and reduces the pressure of paper records storage. It is a practical and effective way of controlling patient records.

Objective To summarize the clinical and EEG characteristics of benign epilepsy of childhood with centro-temporal spikes(BECCT). Methods The clinical and EEG data of 78 children with BECCT were retrospectively analyzed. Results The onset age of this disease was 3 to 14 years, and 62 patients aged 5 to 10 years(62/78,80%). The seizures of 72 cases(72/78,92.3%)were associated with sleep. 63 cases(73/78,80.8%)were partial seizures, and 15 cases(15/78,19.2%) were generalized seizures. EEG demonstrated that there were sharp or spike waves of interior discharge in the centro-temporal area of one or two sides, 55.4% cases were positive in awake EEG, and 92% cases were positive in sleep EEG. 93.2% cases were responsive to antiepileptic drugs. Conclusion The characteristics of BECCT were as follows: partial epilepsy related to sleep, partial seizure types and sharp or spike waves of EEG in centro-temporal area. EEG sleep induction test could increase positive diagnostic rate of this disease, and the prognosis of this disease was fine. The early diagnosis is helpful for the patients' prognosis, and can alleviate psychological suffering of the patients and their families.