DNA methylation is one of the most common epigenetic events in eukaryotic cell.Aberrant DNA methylation is tightly related to tumorigenesis.Recent evidence indicates that DNA methylation is a promosing biomarker for cancer.The cancer-associated DNA methylation events could be detected from serum(plasma),urine,stool and bronchoalveolar lavage,providing a new choice for noninvasive cancer diagnosis.In this review,we summarized the advances on DNA methylation for noninvasive cancer diagnosis.

Two hundred and forty patients with primary hypertension in Weifang Community were randomly sampled as contract group and another 240 hypertensive patients were sampled as control group.Patients in contract group signed service contract with general practitioners (GPs),who provided individualized service for hypertension management.Patients in control group receive conventional outpatient service for hypertension management.The clinical data of patients before and one year after contracted services were documented and compared with those in control group.The results showed that in contract group the average systolic pressure decreased from 134.60 mm Hg (1 mm Hg =0.133 kPa) to 130.62 mm Hg (P =0.000),reaching-the-target-BP rate increased from 56.3％ to 85.5％ (P =0.000),serum total cholesterol and triglyceride decreased significantly one year after receiving contracted services (P =0.000) ; while these indicators were not significantly changed in control group (all P ＞ 0.05).The results indicate that the contracted services of GPs can improve the effectiveness of hypertension management in community.

We studied policies of traditional Chinese medicine (TCM) on four levels ( national,Shanghai city, Pudong New District and community health services (CHS)) from 2008 to 2009 with the method of literature review,questionnaire survey and group interview. 18 national, 5 Shanghai's, 4 Pudong New District's and 87 CHS' TCM related policies were identified. By defining the development target,financial capability, human resource and proper technology of TCM care, those policies ensured and prompted the progression of community TCM services. Yet TCM policy fulfill in community remained lagged,and talent cultivating, medical insurance support and TCM participating in public health needed to be improved.

Aim To explore the effect of atorvastatin on NF-?B/I?B signal pathway,P selectin and ICAM-1 expression in cholesterol-rich diet-induced rabbit atherosclerosis.Methods 24 New Zealand white rabbits were randomly divided into three groups: control group,high-cholesterol group and atorvastatin group.Nucleus NF-?B p65 and cytoplasma I?B? were detected with Western blot technique.Plasma P selectin was detected with Immunoradioassay.Immunohistochemistry was employed to detect the expression of ICAM-1.Results Compared with the control group,the expression of nucleus NF-?B p65,P selectin and ICAM-1 increased significantly and cytoplasma I?B? reduced in high-cholesterol group(P

Objective To study the relationship between overweight, obesity and blood pressure among children living in urban Beijing. Methods A total of 4 267 pupils aged 9～10 years were randomly selected. Overweight and obesity were defined by age and sex specific BMI classification reference for Chinese children. Hypertension was defined by age, sex and height specific reference for US children. Results The prevalence of overweight and obesity in boys was 19.4% and 21.2%, in girls was 10.9% and 12.1%, respectively. Mean levels of systolic blood pressure (SBP) and diastolic blood pressure (DBP) have significant different between all study group. The total prevalence of high blood pressure (HBP) was 16.1% for boys and 15.2% for girls, while it was 36.4% and 41.3% among obese boys and girls, respectively. The prevalence of high systolic blood pressure (HSBP), high diastolic blood pressure (HDBP)and HBP gradually increased as weight increased. Compared with the normal weight group, OR(95%CI) for HBP of overweight and obese children were 2.6 (2.1～3.3) and 5.8 (4.7～7.0), respectively. Conclusion Obesity is positive correlation with hypertension in children aged 9～10years of Beijing, and the risk of hypertension was increased with their body weight.

Normal perfusion pressure breakthrough is a serious complication that may occur in the treatment of cerebral arteriovenous malformations. This article reviews the progress in research on normal perfusion pressure breakthrough in recent years. It mainly investigates its pathogeneses, predictive methods and prevention and treatment strategies.

Objective To explore the changes of cognition and sleep status in the youngsters with internet addiction disorder (IAD), and to clarify the relation between cognition and sleep-related indicators of IAD youngsters.Methods Event related potential (ERP) and Pittsburgh Sleep Quality Index (PSQI)test were used for forty-two IAD (case group)and forty non-IAD (control group) in youngsters, and the indexes of period latency (PL)of N1 ,P2 ,N2 ,P3 and amplitude of P3 (P3 amp)of ERP were analyzed,the factor scores of sleep quality (SQ), sleep latency (SL), sleep time (ST), sleep efficiency (SE), sleep dysfunction (SD), sleeping pills (SP), daytime dysfunction (DD)and total score of PSQI were analyzed. The correlation between the P3 PL, P3 amp of ERP and all the indexes of PSQI in case group was analyzed.Results The PL of N2 ,P3 of ERP in case group was significanly longer and the P3 amp was significantly lower than those in control group (P<0.01);the factor scores of the SQ,SL,ST,SE,SD,DD,and the total score of PSQI test had significant differences between two groups (P<0.01).The correlation analysis results showed that there were remarkable positive correlations between the P3 PL and the factor seores of the SQ,SL,ST,SE,SD,DD and the total score of PSQI test in case group (r=0.46,0.34,0.51,0.40,0.48,and 0.54;P<0.01),and there were remarkable negative correlations betweeb P3 amp and the indexes of PSQI test in case group (r=-0.42,-0.45,-0.49,-0.38,-0.38,and -0.50;P<0.01).Conclusion The IAD youngsters have cognition and sleep dysfunction,and the both factors can affect each other.

Objective To explore the relation between personality characteristics and event related potential P300 with internet addiction disorder(IAD) of youngsters.Methods Eysenck Personality Questionnaire and event related potential test were used for forty-two IAD and forty non-IAD in youngsters,and the results were compared between the two groups.Results The latency of N2,P3 ((267.2±24.2) ms,(374.5 ±22.0) ms) of event related potential in IAD group were significantly longer and the amplitude of P3 ((3.9± 2.5) μV) was significantly lower than those in non-IAD group((235.7±21.8) ms,(332.3±23.5) ms,(7.8±2.4) μV) (P＜0.01).And the indexes of P,N score (56.2±9.4,49.8±7.4) of Eysenck Personality Questionnaire were significant higher than those in non-IAD group(51.9±9.7,42.4±7.5)(P＜0.01),but the indexes of E,L score (45.7±9.0,44.3±7.9)of Eysenck Personality Questionnaire were significant lower than those in non-IAD group(51.2±9.4,50.9±8.3) (P＜0.01).Correlation analysis showed that there were remarkable positive correlation with latency of P300 and P,N score of Eysenck Personality Questionnaire respectively in IAD group(r=0.49,0.54,P＜0.01),and a significant negative correlation with E,L score(r=-0.43,-0.46,P＜0.01),there was a remarkable negative correlation with amplitude of P300 and P,N score of Eysenck Personality Questionnaire respectively in IAD group(r=-0.41,-0.50,P＜0.01),and a significant positive correlation with E,L score(r=0.45,0.47,P＜0.01).Conclusion IAD in youngsters has personality characteristics and cognitive dysfunction,and it has a relation with personality and event related potential P300.

Objective To define basic medical service items in Shanghai community health centers.Methods Two rounds of Delphi expert consultation were carried out with 36 experts specialized in general practice,general practitioner training,internal practice,public health and health management,then the scope of medical service system was set up based on experts' opinions.Results The newly-defined system consisted of three first-grade items:outpatient medical service,hospital medical service and medical service for home beds and temporary home visits.The first-grade items contained 22 second-grade items and the second-grade items consisted of 26 detailed items,including 17 baseline items,8 optional items and 1 restrained item.In two rounds of Delphi consultation the activity coefficient of experts was all 100％,authority coefficients of experts was 0.825,and harmonious coefficient of the importance of the evaluation was 0.361 and 0.488 (all P ＜ 0.01),respectively.Conclusion The preliminarily developed system of basic medical service items in Shanghai community health service can provide a reference frame for government policy-making.

We investigated health condition and evaluate the viability of Barthel's measuring scale for 1297 elder patients at 17 nursing homes.We nursed all patients by standards and performed a comparison study.The nursing percentages of highest and lowest levels were higher than the previous ones ( P ＜ 0.01 ),while the nursing percentage of normal level was lower than the previous one ( P ＜ 0.01 ).The cooperation between doctors and nurses became optimized through progressive patient care.Ideal nursing standard should be based upon the doctors' judgment and nurses' evaluation.The new standard will be more suitable for patients and easier to control for nurses.