Objective To evaluate the safeness of this treatment by comparing the serum IGF-Ⅰ and IGFBP3 concentration level before and after receiving rhGH treatment in pediatric patients.Methods 30 pediatric patients in our hospital receiving rhGH treatment were subjected to monitor serum IGF-Ⅰ and IGFBP3 level before and after the treatment and calculate the IGF-Ⅰ and IGFBP3 ratio.At the same time the serum T3,T4 and TSH are also assayed.Results The average increase of booly heights was(8.77?3.01)cm /y(t=7.773,P0.05),but the serum IGFBP3(t=3.759,P0.05).However,there was statistical significance of the serum IGFBP3 level between subgroups(f=22.964 P0.05).Conclusion The serum IGF-Ⅰ and IGFBP3 level are able to be used as one of clinical evaluation benchmarks to detect the risks of inducing tumor during the rhGH treatment in the pediatric patients.

Objective To study the relationship between overweight, obesity and blood pressure among children living in urban Beijing. Methods A total of 4 267 pupils aged 9～10 years were randomly selected. Overweight and obesity were defined by age and sex specific BMI classification reference for Chinese children. Hypertension was defined by age, sex and height specific reference for US children. Results The prevalence of overweight and obesity in boys was 19.4% and 21.2%, in girls was 10.9% and 12.1%, respectively. Mean levels of systolic blood pressure (SBP) and diastolic blood pressure (DBP) have significant different between all study group. The total prevalence of high blood pressure (HBP) was 16.1% for boys and 15.2% for girls, while it was 36.4% and 41.3% among obese boys and girls, respectively. The prevalence of high systolic blood pressure (HSBP), high diastolic blood pressure (HDBP)and HBP gradually increased as weight increased. Compared with the normal weight group, OR(95%CI) for HBP of overweight and obese children were 2.6 (2.1～3.3) and 5.8 (4.7～7.0), respectively. Conclusion Obesity is positive correlation with hypertension in children aged 9～10years of Beijing, and the risk of hypertension was increased with their body weight.

Objective To analyze the determinants of television viewing among people in China,and to provide basic information for developing intervention strategies.Methods The data of 66 601 subjects aged 6 years and over(male 32 254,female 34 347) on television viewing from 2002 China National Nutrition and Health Survey was used.Results The prevalence of watching TV 2h and over among Chinese residents was 60.6%.Female had a decreased likelihood of watching TV 2h and over(adjusted odds ratio,AOR: 0.78).Children aged 13～17 years had a decreased likelihood of watching TV 2 h and over(AOR: 0.71) compared with younger children,however,adults had an increased likelihood(AOR: 1.34 for 18~44 yr,1.16 for 45～59 yr,1.04 for 60 yr and over).Light intense occupations were associated with an increased likelihood of watching TV 2 h and over(AOR: 7.18).Higher family income had an increased likelihood of watching TV 2 h and over(AOR: 1.12,1.07).Highest education level was associated with a decreased likelihood of watching TV 2h and over(AOR: 0.91).Engaging in exercise was associated with a decreased likelihood of watching TV 2h and over(AOR: 0.74).Conclusion Age,gender,socioeconomic status and exercise can influence the time spending on TV among Chinese residents.

Objective To describe the status of sleeping time among Chinese population.Method The data of 2002 China National Nutrition and Health Survey was used for the analysis.197 954 subjects aged 6 years and above were involved.Results The average daily sleeping time of the domestic Chinese was 8.3 hrs.In groups of 6～12 yrs,13～17 yrs,18～44 yrs,45～59 yrs,60 yrs and above,the daily sleeping time were 9.1 hrs,8.5 hrs,8.2 hrs,7.9 hrs and 7.8 hrs,and the rate of insufficient sleeping was 69.0%,58.5%,4.1%,9.2% and 17.1%,respectively.The rate of excessive sleeping in groups of 18～44 yrs,45～59 yrs and 60 yrs and above were 27.1%,20.2%,24.3%,respectively.Conclusion The status of insufficient and excessive sleeping existed in Chinese at the same time,and further research should be developed on the association between sleeping time and health.

Objective To study the characteristics of peripheral nerve conduction (PNC) in advanced-stage amyotrophic lateral sclerosis (ALS) patients undergoing mechanical ventilation.Methods The sensory conduction velocity (SCV),motor conduction velocity(MCV),distal motor latency(DML) and amplitude of compound muscle action potential(CMAP) were determined in three ALS patients undergoing mechanical ventilation.The clinical data and PNC characteristics of the patients were analyzed.Results The 12 motor nerves were abnormal in all the three patients,including absence of responsive wave (66.7%,8/12),decreased amplitude of CMAP(33.3%,4 /12),prolongation of DML(16.7%,2 /12) and reduction of MCV(16.7%,2 /12).The CMAP amplitude of patients gradually decreased with the progression of disease,and finally led to adverse reactions.The SCV and amplitude of sensory nerve action potential were normal in all the three patients.Conclusion ALS patients undergoing mechanical ventilation may have severely damaged motor nerve conduction,and their sensory nerve is generally not affected,with the specific reason remains to be further studied.

Objective To summarize the clinical, electrophysiological, pathological characteristics of chronic inflammatory demyelinating polyradiculoneuropathy (CIDP). Methods From Jan 1990 to Dec 2003, 32 patients of CIDP were admitted by our hospital, 14 male and 18 female. The age range of onset of illness was 13 to 74 years old (averaging 42.56) with the prime period of 40 to 50. Before hospitalization, the course was from 2 months to 5 years. Among all patients, there were 8 cases of a relapsing course and 24 cases of a chronic progressive course. Twenty-four patients were treated with corticosteroids, while 7 received immuglobin (IG) and corticosteroids. The clinical data before and after the treatment was studied retrospectively. Results Most of the patients had subacute or chronic onsets. The common initial symptoms are numbness, paresthesia, and extremity weakness. Diplopia, decreased visual acuity, dysarthria, and dysphagia could also be found initially. It was usually a symmetric sensorimotor neuropathy with either a relapsing course or a chronic progressive course. It could be accompanied with autonomic dysfunction and cranial nerve involvement. Electromyogram demonstrated that the motive and sensory nerve conduction velocities were slow. The sural nerve biopsy showed demyelination and remyelination. `IG and corticosteroids were both effective. Conclusion CIDP might result in widespread peripheral nerve damages, in which autonomic dysfunction and cranial nerve involvement were common. The dominant electrophysiological changes showed peripheral nerve demyelination accompanied by axon degeneration. The sural nerve biopsy played an important role of diagnosis. The treatment with IG and corticosteroids was a most effective way for CIDP nowadays.

Objective To explore the expression of ErbB3 in osteosarcoma cell lines Saos-2 and its significance.Methods Real-time quantitative PCR was used to detected ErbB3 mRNA expression in osteosarcoma cell lines Saos-2 and normal human osteoblasts cell lines N704,and Western blotting was used to detected ErbB3 protein expression.Short hairpin RNA was used to construct ErbB3 knockdown cells and the cell count in 0-3 d was detected.The 48 h survival rates of ErbB3 knockdown cells and normal Saos-2 cells were detected in the presence of 0-100 μmol/L paclitaxel.Results Real-time quantitative PCR showed significant enhanced ErbB3 mRNA expression in Saos-2 cells compared with N704 [(4.15 ± 0.04) times,t =7.31,P ＜0.05],and Western blotting showed significant enhanced ErbB3 expression in Saos-2 cells compared with N704.As compared with normal Saos-2 cells,ErbB3 knockdown could reduce the Saos-2 cells proliferation,at the first three days in culture,the Saos-2 cell number of ErbB3 silent was (22.2 ± 2.9) thousand,and the control was (45.8 ± 4.1) thousand,with statistical significance (t =8.23,P ＜ 0.05).Moreover,ErbB3 knockdown could reduce the Saos-2 cells tolerance to paclitaxel,when treated with 20 μmol/L paclitaxel,surviving cells in ErbB3 silent group was (43.2 ± 4.7) ％,and the control was (61.4 ± 5.9) ％,with statistical significance (t =6.74,P ＜ 0.05).Conclusion ErbB3 highly expresses in Saos-2 cells,ErbB3 expression can enhance the proliferation of Saos-2 cells and the tolerance of Saos-2 cells to paclitaxel.

Objective To analyze the features of nerve conduction in patients with amyotrophic lateral sclerosis (ALS),and explore the correlation between compound muscle action potential (CMAP)amplitude and disease duration and revised amyotrophic lateral sclerosis functional rating scale (ALSFRSR).Methods Standard motor and sensory nerve conduction studies were performed in 154 patients with ALS.The following parameters were collected including CMAP amplitude,distal motor latency (DML),motor conduction velocity,sensory conduction velocity and sensory nerve action potential amplitude.Regression study was done to explore the correlation between CMAP amplitude and disease duration and ALSFRS-R.Results Motor nerve conduction abnormalities were presented in a majority of the patients with prolonged DML in the tibial nerve,median nerve and ulnar nerve as the most common form (61.06％-81.42％),followed by decreased CMAP amplitude (30.12％-53.98％),decreased MCV (12.05％-16.81％) and absence of CMAP (2.65％-9.73％).Sensory nerve conduction abnormalities were detected in a small proportion of patients and the decreased SCV,decreased SNAP amplitude and absence of SNAP in the sural nerve,median nerve and ulnar nerve were found in 1.22％-2.73％,0-1.82％ and 0-1.22％patients respectively.No correlation was found between CMAP of the common peroneal nerve,tibial nerve,median nerve and ulnar nerve and the disease duration (P ＞ 0.05),while significant positive correlation was established between CMAP amplitude of the median nerve and ulnar nerve and ALSFRS-R (r =0.273,P =0.016;r =0.357,P =0.001).Conclusions Motor nerve conduction is abnormal in a majority of ALS patients with prolonged DML as the most common form,while abnormal sensory nerve conduction is only found in a few of ALS patients.CMAP amplitude of the median nerve and ulnar nerve might be of certain clinical value in evaluating the severity of ALS.

Objective: To design the “one-year physical activity questionnaire” used in 2002 China National Nutrition and Health Survey. Methods: The intensity of each activity in the questionnaire was assessed from literature review and experts consultation. The energy intake was calculated from food frequency questionnaire for validation of the physical activity questionnaire. Results: The average daily energy expenditure per capita estimated from the questionnaire was 11.6MJ (male 11.9MJ, female 11.2MJ). The average physical activity level (PAL) was 1.95 (male 1.88, female 2.02). There was significant correlation between energy expenditure and energy intake (r = 0.13, P

Objective: To describe the physical activity level and its influencing factors of Chinese professionals, to provide basis for developing intervention strategies. Method: The information on physical activity was collected by one year physical activity questionnaire in "2002 China National Nutrition and Health Survey", and 29 783 professionals aged 18-59 years old(male 15 911, female 13 872)were involved. Metabolic equivalent (MET) was adopted to estimate the intensity level of physical activities. Physical activity level (PAL) was calculated to classify sufficient (PAL≥1.7) or insufficient (PAL