Objective To identify the genotype of Toxoplasma gondii isolated strains from a congenital teras（KS strain）and an HIV⁃Toxoplasma co⁃infected patient in Jiangsu Province. Methods T. gondii DNA of tachyzoites of a isolate from a congenital teras（KS strain）and blood DNA of an HIV⁃Toxoplasma co⁃infected patient in Jiangsu Province were extracted，and 11 loci were identified for the genotype by polymerase chain reaction restriction fragment length polymorphism（PCR⁃RFLP）. Results The complete bands were obtained from the congenital teras（KS strain）and HIV⁃Toxoplasma co⁃infected patient in Jiangsu Province，and identified as T. gondii gene type I. Conclusion T. gondii gene type I may be the dominant genotype strain of T. gondii among the women who have the abnormal pregnant outcomes and HIV⁃Toxoplasma co⁃infected patients in Jiangsu Province.

Objective:To investigate the clinical efficacy of ultrasound-guided vacuum-assisted excision (VAE) in the treatment of breast benign phyllode tumor and the risk factors of local recurrence after operation.Methods:A total of 256 cases of patients with breast benign phyllode tumor admitted to Yuncheng Central Hospital from January 2012 to January 2020 were included in the retrospective study, they were all female, of which 160 cases received ultrasound-guided VAE surgical treatment and 96 cases received open surgical treatment. Measurement data were expressed as mean ± standard deviation ( ± s), and t-test was used for comparison between groups. The Chi-square test or Fisher exact probability method was used to compare the data groups. Univariate analysis was performed on the factors related to the local recurrence of benign phyllode tumor after surgery, and then the factors with statistically significant differences in the univariate analysis were further incorporated into multivariate Logistic regression analysis. Results:Univariate analysis results showed that the maximum diameter of tumor ≥25 mm and the history of ipsilateral breast fibroadenoma were associated with postoperative local recurrence of breast benign phyllodes tumor ( P<0.05). Multivariate Logistic regression analysis results showed that tumor diameter ≥25 mm was an independent risk factor for postoperative local recurrence of breast benign phyllodes tumor ( P<0.05). Conclusion:Ultrasound-guided VAE in the treatment of breast benign phyllodes tumor patients with maximum diameter less than 25 mm can reduce the postoperative local recurrence rate, and patients with tumor maximum diameter greater than 25 mm should have the higher local recurrence risk.

Objective:To analyze the articles in the research field of auricular acupoint therapy in the treatment of stroke sequelae in our country, understand the status of the articles and the research focus, and analyze the research trends in this field.Methods:Using China National Knowledge Infrastructure (CNKI) , Wanfang Data, VIP database, China Biology Medicine Literature Database, PubMed, Web of Science as data sources, we retrieved the research articles on the application of auricular acupoint therapy for the sequelae of stroke, the retrieval time was from January 1995 to November 2020. We performed data statistics on the retrieved documents and analyzed the external characteristics and internal attributes of the articles.Results:A total of 402 articles were included, including 371 journal papers and 31 dissertations. From 1995 to 2020, the average annual number of articles published on the treatment of sequelae of stroke by auricular acupoint therapy was 16 articles, and the number of articles increased significantly since 2008. The first author was located in 23 provinces, 4 municipalities, and 3 autonomous regions in my country. The top 3 regions in terms of publication volume were Guangdong, Zhejiang and Jiangsu. General hospitals were the majority of research institutions, accounting for 50.7% (204/402) . Articles were mainly published in Chinese medicine and comprehensive medical journals, and nursing journals only accounted for 10.8% (40/371) of the total publication volume. The average number of citations per article was 6.36 times, which was lower than the average number of citations for domestic articles of 8.14 times. A total of 80 (19.9%) articles were funded by the fund, and were relatively concentrated in the developed areas in the central and eastern regions. The research content of the articles was mainly on auricular acupoint therapy for post-stroke constipation, insomnia and depression, and the main acupoints commonly used were Shenmen, subcortical, heart, sympathetic, liver and kidney.Conclusions:Auricular acupoint therapy for the treatment of stroke sequelae is gradually receiving attention. The main research area is the economically developed areas in the central and eastern regions. Teamwork needs to be strengthened, and the number of documents and academic influence is low, and the quality of articles needs to be improved, and there is an imbalance in the distribution of dissertation funds. The focus of treatment is constipation, insomnia, and depression, which is 3 types of stroke sequelae. The application of auricular acupoint therapy combined with other therapies is the main trend of research.