1.Analysis on internationalization status of traditional Korean medicine
International Journal of Traditional Chinese Medicine 2011;33(12):1090-1092
This article reviewed the internationalization history of the Traditional Korean Medicine (TKM).It expounded the current situation of TKM's international standard application for WHO and International Standard Organization (international organization for standardization,ISO),and the competition between Traditional Chinese Medicine (TCM) and Traditional Korean Medicine.The authors presumed the essence content of TCM should be included in multiple international standards as early as possible to promote the intemationalization of TCM.
2.Cognition and Self-Rated Health in Military Personnel in High Altitude Area
Jingbo ZHAO ; Yaning XIE ; Jun XU
Chinese Mental Health Journal 2002;0(11):-
Objective: To investigate the relationship between the cognition and the self-rated health in military personnel in high altitude area. Methods: 490 military personnel in high altitude area were assessed with Self-rated Health Measurement Scale and the questionnaire of cognitive appraisal to environment and service. Results: The scores of the self-rated health in military personnel in high altitude area were higher than average(6.5~7.9, full score is 10); The main factors perceived by soldiers as having negative influence on health were poor communication with outside (53.6%), lack of opportunity of contact with opposite sex(51.8%), lack of amusement(48.4%)and service of hygiene(46.2%). Correlation analysis showed the cognitive appraisal factors were correlated with the self-rated health significantly(P
3.Expression and polyclonal antibody preparation of human XTP3 gene
Hao XU ; Longfeng ZHAO ; Jun CHENG
Medical Journal of Chinese People's Liberation Army 2001;0(07):-
Objective To construct prokaryotic expression vector of XTP3 gene and induce the expression of fusion protein in E.coli,and prepare XTP3-specific rabbit polyclonal antibody,detect the specificity of the antibody in hepatic carcinoma tissue and normal liver tissue.Methods DNA fragment of XTP3 amplified by PCR was inserted into pET-32a(+) to construct prokaryotic expression vector pET-32a(+)-XTP3.After identified by sequencing,pET-32a(+)-XTP3 was transformed into E.coli BL21 and induced with IPTG.After analyzed by SDS-PAGE and Western blotting,the induced expression product was purified and renatured by Ni+ affinity column chromatography.The purified protein was used to immunize New Zealand rabbits to gain polyclonal antibody,and the polyclonal antibody was then detected by ELISA,immunohistochemistry and Western blotting.Results Prokaryotic expression vector pET-32a(+)-XTP3 was successfully constructed,and the XTP3 fusion protein of about 52kD was highly expressed in E.coli.DS-PAGE showed that the protein product was mainly in inclusion body.The purified protein and polyclonal antibody were obtained successfully.It was manifested by ELISA that the titer of polyclonal antibody was over 1∶128 000.Immunohistochemistry showed that XTP3 antibody presented membrane-positive in hepatic carcinomous tissue.Conclusions The recombinant XTP3 protein and polyclonal antibody have been obtained successfully.These results lay a foundation for studying the immuneogenicity and bionomics of XTP3 protein.
4.Analysis of the Reliability and Validity of SRHMS V1.0 Applied to Measuring Person's Self-rated Health in Shenzhen
Jun XU ; Lexuan LUO ; Jingbo ZHAO
Chinese Journal of Clinical Psychology 2000;0(04):-
Objective: To assess the reliability and validity of SRHMS V1.0 applied to measuring person's self-rated health in Shenzhen.Methods: 5940 individuals received SRHMS V1.0 assessment in Shen Zhen.Results: Cronbach ?琢 of SRHMS V1.0 was 0.924.Cronbach ?琢 of SRHMS V1.0's each dimension of three subscales was from 0.665 to 0.877.The correlation coefficient was 0.604 of split-half scores of SRHMS V1.0.There was a high correlation between each item of SRHMS V1.0 and its dimension,but a low correlation between items and other dimension.Dimension scores correlated significantly with its subscale,and low with other subscale.Factor analysis produced nine main factors that were basically consistent with original theoretical conception about SRHMS V1.0.The correlation of global scale score?three subscales scores and each item were also statistically significant,respective.Conclusion: The results show that SRHMS V1.0 is reliable and valid.
5.The cause and prevention of positive stump in gastric cancer
Jun ZHAO ; Yougang MA ; Peihong XU
Journal of Clinical Surgery 2001;0(02):-
Objective To discuss the cause and preventive measure of positive stump in gastric cancer.Method The clinical data of 426 patients with residual cancer after resection of gastric cancer were retrospectively analyzed.Result In these cases 37(8.7%,37/426) were found to have residual cancers at the margin of resection.Conclusion The incidence of residual cancer at the cut edge is closely related to gross type,size of cancer,tumor stage and degree of differentiation.
6.Application of OCT measurement of macular GCC and RNFL thickness around optic disc in the diagnosis of early glaucoma
Jun, ZHAO ; Man, XU ; Ying, ZHOU
International Eye Science 2017;17(7):1289-1292
AIM: To study the application of macular ganglion cell complex (mGCC) and peripapillary retinal nerve fiber layer thickness (pRNFL) measured by optical coherence tomography (OCT) in the early diagnosis of glaucoma.METHODS: Case-control study.Eighty-six subjects, including 30 eyes in normal subjects, 27 eyes in suspected primary open angle glaucoma, 29 eyes in primary open angle glaucoma were enrolled in this study.The thickness of mGCC and pRNFL were measured by OCT.The area under the receiver operating characteristic(AROC) curve at fixed specificities were calculated for each parameter.RESULTS: There were significant differences in mean pRNFL thickness, superior pRNFL thickness and inferior pRNFL thickness between normal group, suspected glaucoma group and early glaucoma group (P=0.001, 0.004, 0.011).The mean mGCC thickness, the thickness of the top mGCC, the thickness of the lower mGCC were statistically significant (P=0.008, 0.002, 0.003);the difference of general loss of volume (GLV) and focal loss of volume (FLV) between the three groups was statistically significant (P=0.002).Compared with the normal group, all the pRNFL and the mGCC parameters were higher in the suspected glaucoma group, and the FLV had the highest AROC (0.801), all the remaining AROC was >0.700 except above Prnlf (0.688).Compared with the normal group and the early glaucoma group, all the pRNFL and the mGCC had higher AROC, average mGCC was hightest(0.804), all parameters AROC were >0.700 except mean pRNFL (0.683).In suspected glaucoma group, 58% patients had abnormal mGCC thickness and 23% had abnormal pRNFL thickness;in early glaucoma group, 98%patients had abnormal mGCC thickness and 90% had abnormal pRNFL thickness;in normal group, 93%patients had abnormal mGCC thickness and 93%had abnormal pRNFL thickness, the correlation between the three groups was statistically significant (x2=12.11, P<0.05).CONCLUSION: OCT measurement of mGCC thickness and pRNFL thickness in early glaucoma have good diagnostic ability;mGCC thickness measurement can be used as an effective method for early diagnosis of glaucoma.
7.STUDY ON CELL MEDIAED-IMMUNITY INDUCED BY PORIN OF SALMONELLA TYPHIMURIUM
Yuewu ZHAO ; Fenglan ZHAO ; Jun HU ; Youmei XU
Chinese Journal of Zoonoses 2000;(6):45-47
AimTo investigate cell mediated-immunity induced by porin of Salmonella typhimurium(STM). Methods Level of delayed-type hypersensitivity(DTH), IL-2 of immunized BALB/c mice were determined by footpad swelling,MTT and transfered protective rate of T cells purified by neloy wool were studied with 500LD50 of the bacteria(intraperitoneally). Results A marked level of DTH(3. 6±0. 2,P<0. 01),IL-2(26.2±4.9,P<0. 01) and the immuinized T cells transfer protec tive level (42.9% ,P<0. 01) was induced by porin from STM. Conclusion These results indicated that porin of STM might in duced stronger CMI in BALB/c mice.
8.Impact of magnetic attachment keepers on magnetic resonance imaging examination
Xuezhu ZHAO ; Yuhua LIU ; Jun XU ; Hongbin HAN ; Xiaojuan XU
Journal of Peking University(Health Sciences) 2004;0(01):-
Objective:To evaluate the effects of magnetic attachment keepers on MRI images.Me-thods:In in vitro part,keepers of MAGFIT EX 400,MAGFIT EX 600 and MAGNEDISC 800 magnetic attachment systems were cast into standard root-caps with different alloy respectively,including nickel-chromium alloy and gold-palladium alloy,or not be cast for contrast.In in vivo part,volunteers with keepers in different position and amount were included.All the specimens and volunteers were imaged by a Siemens SONATA 1.5-T MRI scanner.Extent of the artifacts in every slice was measured.The magnitude of the artifacts and the distortion of the anatomic structures were compared.Results:All kinds of keepers being studied produced obvious MRI artifacts.The extent of MRI artifacts induced by MAGFIT EX 400 keepers,MAGFIT EX 600 keepers and MAGNEDISC 800 keepers are 158.94 mm,168.52 mm and 173.00 mm,respectively.The images of mental region,tongue,palate,jawbone and sinus were evidently obscured by artifacts in all cases.When keeper was put in the molar region,the keeper-related MRI artifact may obscure the imaging of brain,spinal cord.SE sequence with right-left frequency-encoding direction was more desirable in MRI for the patients with magnetic attachment keepers.Conclusion:Keepers of magnetic attachment system did induce obvious artifacts in MRI examination.
9.Integrative treatment of multiple trauma patients with severe craniocerebral injury in emergency center (with 148 cases report)
Guangyu WU ; Hongbo XU ; Feng ZHAO ; Lijun TAO ; Jun FANG
Chinese Journal of Postgraduates of Medicine 2006;0(35):-
Objective To explore the significance and important measure of integrative treatment of multiple trauma patients with severe craniocerebral injury in emergency center. Methods One hundred and forty-eight multiple trauma patients with severe craniocerebral injury in emergency center from November 2002 to February 2006 were analysed retrospectively. Result In total 148 cases, 72 were cured and 26 dead, 7 were in status of plant man, 17 experienced severe deformity, and 26 did mild deformity. Conclusion Multiple trauma patients with severe craniocerebral injury usually experience a serious situation and rapid development. Therefore emergency doctors are required for organizing salvage of such patients, at the same time treatment and diagnosis are implemented. Firstly the most important key to successful salvage is appropriate disposal of fatal injury and early elimination of shock. Proper surgical choice, especially at the first time, inspection and protection of visceral functions, and attention to nutritional support are other vital methods to gain more successful salvage. ICU is also emphasized for its essentiality.
10.EFFECT OF TOTAL GLUCOSIDES OF PAEONY ON LEUKOTRIENE B_4 BY PERITONEAL MACROPHAGES IN RATS
Jun LI ; Weizhong ZHAO ; Minzhu CHEN ; Shuyun XU
Chinese Pharmacological Bulletin 1987;0(01):-
Total glucosides of paeony ( TGP ) , an extract of Paeonia Lacti-flora Pall were investigated for its effect on the synthesis of leukotriene B_4 ( LTB_4 ) by peritoneal macrophages ( PM?). LTB4, generated by 10~9~15 ? 10~10/L PM? with ionophore A_23187 , were measured with RP-HPLC. Suppression of releasing LTB_4 from PM? by TGP ( 100 mg/L ) was as much as the same dose of Flufenamic acid, but the action of TGP was slower. TGP significantly inhibited the synthesis of LTB_4 by PM? in a dose-dependent manner. The concentration of TGP required to obtain 50% inhibition ( IC_50 ) of formation of LTB_4 was 0.66mg/L. The data suggested antiinflam-matory and immunomodulatory action of TGP were related to its suppression of releasing LTB_4