1.Application and progress of surgical nutrition
Chinese Journal of General Surgery 2001;0(08):-
Nutrition support is extensively used in many clinical fields. The rest energy requirement in Chinese is only 5021~5858kJ/d.In our country 40% to 50% of hospitalized patients need nutrition support.In nutrition support,attention should be paied to :(1)The energy giving to the patient should be appropriate;(2)hormone resistance;(3)immunonut rition.
2.Pseudomonas aeruginosa separation and resistance change analysis in our hospital from 2007 to 2011
Clinical Medicine of China 2013;(4):370-373
Objective To understand the distribution and drug resistance change trend of pseudomonas aeruginosa (PA) in hospital clinical infection in order to provide laboratory basis for rational clinical use of medications.Methods Statistical analyses was conducted retrospectively in 818 strains of PA separated in our hospital from January 2007 to December 2011.Results Of all the separation,PA number of the pathogen constituted a ratio of 15.2% (818/5365) ; The main source of specimens was sputum,accounting for 82.6% (676/818).There was good antimicrobial activity of Imipenem,Piperacillin/Tazobactam,Aztreonam,Ceftazidime,Levofloxacin and Ciprofloxacin to PA,with resistance rates of 14.9% (97/653),25.0% (179/716),26.8% (179/667),26.9% (208/773),28.6% (211/738) and 29.1% (220/757) respectively; The resistance of Ciprofloxacin and Levofloxacin did not change significantly during 2007-2010 year,but in 2011 year a significant decrease of resistance rates was found (ciprofloxacin:35.1% (175/498) vs.17.4% (45/259) ; Levofloxacin:38.0% (181/476) vs.11.5% (30/262)) ; During 2009-2011 year,the resistance of Imipenem and Amikacin increased year by year (Imipenem:7.4% (9/121) vs.7.7% (66/209) vs.19.5% (51/262) ; Amikacin:13.0% (2/23) vs.33.3% (69/207) vs.36.2% (94/260)).Conclusion PA detection rate and its resistance is rising from 2007 to 2011 year.We should make rational drug use clinically according to antimicrobial susceptibility test results.Laboratories shall strengthen the bacterial resistance monitoring.
3.NADPH oxidase and atherosclerosis and ischemic cerebrovascular disease
International Journal of Cerebrovascular Diseases 2012;20(6):468-471
Reactive oxygen species (ROS) caused oxidative stress plays an important role in the occurrence and development of atherosclerosis,and it is associated with the occurrence of ischemic cerebrovaseular diseases.This article reviews the mechanism of action of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase in atherosclerosis and ischemic cerebrovascular disease,and the neuroprotective effects of NADPH oxidase inhibitors.
4.THE GLASS CAPILLARY GAS CHROMATOGRAPHIC METHOD FOR THE ANALYSIS OF FATTY ACIDS
Acta Nutrimenta Sinica 1956;0(01):-
A gas chromatographic method was established for the analysis of fatty acid composition of oils. After methlation of the samples, the retention time of the fatty acid esters in the glass capillary coated with crosslin-ked methyl silicone was closely related to the number of carbon atoms.The reproducibity of this method was satisfactory. The relative standard deviation was 0.4-1.5%. Six kinds of vegetable oils were analyzed by this method, and the results were consistent with those reported by Canadian Cereal Association. For analysis of the free fatty acid composition of becte-ria, the results were comparable with those obtained from thin layer chroma-tography and chemical analysis.
5.Brain blood vessel unexpected companion take place psychological research of obstacle and treat the countermeasure
Chinese Journal of Primary Medicine and Pharmacy 2006;0(03):-
Objective To probe into the relevant factors of unexpected psychological obstacle of blood vessel of brain and treat the countermeasure.Methods To adopt Chinese dense of anxious quantity form(HAMA) brain blood vessel unexpected patient in hospitalled to have illness coming on go on psychological obstacle evaluate for the first time to 132 and use supporting psychotherapy and routine medication mainly.Results Patient accounting for 63.3% had psychological obstacles of 132,the female's incidence of disease was higher than that of the male.The smaller the age was,the apter to present the psychological obstacle the age was.Every symptom had improvement in various degree before and after carrying on the psychotherapy.Conclusion The incidence of the unexpected patient's psychological obstacle of blood vessel of brain is relatively high.To take the effective psychological measure of rehabilitation is helpful to the whole recovery of the unexpected patient of blood vessel to brain.
6.Establishment of an orthotopic autologous liver transplantation model with bile ducts ischemia-reperfusion injury in rats
Academic Journal of Second Military Medical University 1981;0(04):-
Objective:To establish an orthotopic autologous liver transplantation model with bile duct ischemia-reperfusion injury in rats,so as to make it easy for the operation.Methods: Eighty SD rats were used to establish the models.The method used for this model was the same as the one for orthotopic liver transplantation,except for the blood vessel anastomosis and the constant pressure perfusion of portal vein and abdominal aorta.The portal vein and the hepatic artery reperfusion were controlled by artery clamp.Results: The successful rate of the operation for establishing the model was 95%(76/80) and the anhepatic phase was(16?2) min.The periods of hot and cold ischemia and reperfusion could be accurately controlled and the effect of double constant pressure perfusion was good.There was no erythrocyte in the capillary of bile ductal wall under the light microscope after the reperfusion.Conclusion: This technique,simulating the whole process of liver transplantation,is simple and has high successful rate.Besides,it rules out the influence of immune agent on bile ducts impairment,and better reflects the pathophysiological process of bile ducts ischemia and reperfusion injury,providing a novel approach for investigation of bile ducts injury during liver transplantation.
8.Correlation between gene HLA-class I polymorphism and susceptibility to leukemia
Journal of Xi'an Jiaotong University(Medical Sciences) 1982;0(04):-
Objective To explore the correlation between gene HLA-class I polymorphism and susceptibility to leukemia in Chinese Gansu Han people and search for the genes susceptible to leukemia.Methods HLA-A and B alleles polymorphism in 65 patients with leukemia and 48 normal subjects were determined by PCR with sequence specific oligucleotide probe(PCR-SSO).Results The allele frequencies of HLA-A01 and B38 were increased (P
9.Correlation between NADPH oxidase p22phox CYBA-A930G polymorphism and hypertensive cerebral hemorrhage in Chinese Han population in Shanghai area
International Journal of Cerebrovascular Diseases 2014;22(10):751-755
Objective To investigate the correlation between the NADPH oxidase p22phox-A930G polymorphism and hypertensive intracerebral hemorrhage in Chinese Han population in Shanghai area.Methods The patients with hypertensive intracerebral hemorrhage and normal controls were enrolled.The polymerase chain reaction and the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were used to detect the genotypes and alleles of NADPH oxidase p22phox-A930G.Results A total of 128 patients with hypertensive intracerebral hemorrhage and 151 healthy controls were enrolled.The levels of systolic blood pressure,diastolic blood pressure,glucose,and triacylglycerol,as well as the proportions of smoking and alcohol consumption in patients of the hypertensive intracerebral hemorrhage group were significantly higher than those of the control group (all P< 0.05).There were significant differences in AA,AG,and GG genotypes (42.2%,44.5%,and 13.3% vs.63.6%,27.8%,and 8.6%,x2 =12.757,P =0.002) and in A and G allele (64.5% and 35.5% vs.77.5% and 22.5% ;x2 =8.734,P=0.001) frequencies between the hypertensive intracerebral hemorrhage group and the control group.Multivariatelogistic regression analysis showed that systolic blood pressure ≥ 140 mm Hg (1 mm Hg =0.133 kPa) (odds ratio [OR] 13.952,95% confidence interval [CI] 7.242-26.879; P < 0.001),apolipoprotein A ≥ 0.99 mmol/L (OR 3.139,95% CI 1.012-9.733; P =0.048),and AG +GG genotype (OR 2.333,95% CI 1.253-4.342; P=0.008) were the independent risk factors for hypertensive intracerebral hemorrhage.Conclusions Among the Chinese Han population in Shanghai area,the NADPH oxidase p22phox-A930G polymorphism is an independent risk factor for hypertensive intracerebral hemorrhage.
10.Effect observation of using the self-made pressure reduction bilateral nasal oxygen tube to prevent the nasal oxygen tube ear pressure sore for the comatose patients
Chinese Journal of Practical Nursing 2017;33(21):1638-1640
Objective To observe and discuss the effect of using the self-made pressure reduction bilateral nasal oxygen tube to prevent the ear pressure sore for the comatose patients. Methods Totally 86 cases of comatose patients which needed permanent oxygen inhale admitted during Jan 2016 to Dec 2016 were divided into observation group and control group:Each group 43 cases. Observation group used self-made pressure reduction bilateral nasal oxygen tube to inhale the oxygen, control group used the conventional disposable bilateral nasal oxygen tube to inhale the oxygen and the pressure ulcer stage. Results There was no significant difference in Braden score in two groups, the difference was statistically significant (P>0.05) . Observed two groups patients′ nasal oxygen tube ear pressure sore situation, these were only 2 patients happened nasal oxygen tube ear pressure sore in observation group, on the contrary, these were 9 patients happened in control group, occulting probability was higher than observation group significantly. and the extend of the pressure sore is much serious than the observation. Difference was statistically significant (χ2=5.11, P<0.05). Conclusions During the privation of the nasal oxygen tube ear pressure sore for the comatose patients, self-made pressure reduction bilateral nasal oxygen tube can alleviate the ear skin pressure effectively, reduce the pressure sore occur, the effect is obvious, nursing quality is improved, deserve the clinical expansion.