BACKGROUND:With the development of tissue engineering technology, repairing large-area bone defects using tissue-engineered bone has become a hot spot.
OBJECTIVE:To introduce the bone tissue engineering seed cel s, cytokines, as wel as the characteristics of scaffold materials and their vascularization.
METHODS:With the key words of“bone tissue engineering, scaffold, vascularization”in Chinese and in English, respectively, a computer-based search of articles published from January 2000 to January 2012 was performed in CNKI and PubMed databases. Articles with the summary of bone tissue engineering, bone tissue engineering scaffolds and scaffold vascularization were included.
RESULTS AND CONCLUSION:The selection of seed cel s, application of cytokines, scaffold material performance and degree of vascularization in bone tissue engineering has an important influence on the repair of bone injuries. Appropriate seed cel s is the study foundation in bone tissue engineering, cytokines serve as catalysts, and scaffold materials with good three-dimensional structure can promote cel growth and proliferation, tissue ingrowth, osteogenesis and vascularization. Each scaffold has its own inadequacies, so the combination of a variety of materials can reach a combined effect to meet the clinical demand. In addition, it is important to actively seek new material preparation technology and improve the existing methods, in order to create a more excel ent scaffold. But the vascularization is stil a major test for bone tissue engineering. Current methods to promote vascularization of tissue-engineered bone have some defects. For examples, the use of growth factors to promote vascularization can lead to disease progression in patients with metabolic abnormalities during;microsurgical techniques for tissue engineering bone vascularization are easy to cause trauma and deformity at other parts, which is not conducive to the patient’s physical rehabilitation.

Objective To study the influence of the operation through tubular stomach plus mechanical anastomosis on the life quality of patients with esophageal cancer.Methods Seventy patients with esophageal cancer were treated by operation,tubular stomach plus mechanical anastomosis were used.They were followed up for six months.The quality of life was evaluated.Results The average time for the operation was 120 min and the average amount of bleeding was 291.3ml.Compared with before surgery,there was significant difference in emotion,activity,sleep and diet ( P ＜ 0.05 ).Conclusion The operation through tubular stomach plus mechanical anastomosis could significantly improve the quality of life of patients with esophageal cancer.

With the understanding of the function mechanism of RNAi,it has been widely applied in various fields.RNAi is a powerful tool to study gene functions and provides a new technical method for the specific gene treatment as well.The further development of RNAi technique opens up a new way for tumor gene treatment.

Objective To study the effects of treating irritable bowel syndrome (IBS) with modified TongXieYaoFang. Methods 83 IBS patients were randomly recruited into a treatment group (45 patients) and a control group (38 patients). In the control group, Trimebutine, 200mg, and Bifid triple viable capsule, 420mg, 3 times/day was given orally to the patients, while on the basis of that, the treatment group was administrated with modified TongXieYaoFang in addition. Both groups were treated for one course of 4 weeks. Results The total effective rate of the treatment group and the control group was 93.3% and 71.1% respectively, showing significant difference between the two groups (X2=7.2938, P<0.01 ). Conclusion TongXieYaoFang combined with western medicine is effective in treating IBS.

Objective To explore the clinical characteristics of multiple primary carcinoma (MPC) in elderly patients and the effects of postoperative adjuvant chemotherapy on the prognosis and advanced cancer genesis. Methods The study population consisted of 220 elderly patients with malignant tumor. Thirty-seven elderly patients with MPC were selected in the study. The characteristics including onset age, carcinoma sites, effects of postoperative adjuvant chemotherapy, interval and survival time between first and advanced cancer were analyzed. Results of the 220 subjects,37(16.8%) patients were MPC, among whom 2 patients were synchronous multiple primary carcinomas (SMPC) and 35 patients were metachronous multiple primary carcinomas(MMPC). The median ages at onset were 70,77 and 77.5 years old for the first,second and third tumor respectively. Among a total of 82 tumor focus, the most predilection site of MPC was gastrointestinal tract, with a 34.2%(28 cases) in colorectum and a 13.4%(11 cases) in stomach. The postoperative patients were divided into two groups: 23 accepted adjuvant chemotherapy (chemotherapy group) and 14 accepted no adjuvant chemotherapy (no chemotherapy group). The survival time of chemotherapy group was significantly longer than no chemotherapy group (x2= 4.63, P = 0.0427 ). The median intervals between the first and the second cancer were 6 years and 4 years in chemotherapy group and no chemotherapy group, respectively(x2=1.63, P = 0.207), while the median intervals between the second and the third cancer were 7 years and 2 years, respectively(x2 = 4.255,P=0.043). The mediansurvival time of the third cancer postoperative patients were 3.5 years and 7 months in chemotherapy group and no chemotherapy group, respectively (x2 = 4.62, P = 0.0316 ) . ConclusionsPostoperative adjuvant chemotherapy may prolong the survival time and delay the advanced cancer genesis in elderly patients with MPC.

Objective To compare the renal effects of dopamine and dobutamine in patients with sepsis.Methods 90 patients with sepsis were admitted to this study.After resuscitation,each patient was randomly given different vasoactive agent.The changes in urine output,fractional excretion of sodium(FeNa),and creatinine clearance(CCr)were observed.Results The urine output and FeNa in dopamine group were increased significantly as compared with control group and dobutamine group[(3072±480),(2038±515)and(362±522)ml/24h,(3.80±1.09),(2.06±1.14)and(2.10±0.95)%](P＜0.05).Compared with control group and dopamine group,CCr increased significantly in dobutamine group[(79.2±39.1),(50.6±21.8)and(47.4±16.7)ml/min](P＜0.05).Conclusion Dopamine infusion markedly elevates urine output and FeNa,but has no effect on CCr.Dobutamine treatment misht significantly increase CCr,but has no effect on urine output.

With the construction of hospital culture, Shenzhen people's hospital established a fine brand. In the construction of service culture, we take the patients interest in the first place. Through improving the quality of medical staff, and innovating the medical treatment and scientific research, we have got predicted accomplishment. In the paper, present hospital culture is analyzed, and how to develop the culture is discussed.

Objective The study aimed to explore the application of fast track surgery in nursing of patients with colon cancer in a primary hospital.Methods The 60 colon cancer patients were identified with numbers to indicate randomization into the fast tract surgery group (group A) and the conventional treatment group (group B) with 30 cases in each group.Group A used the concept of fast track surgery (FST) perioperative nursing care,whereas traditional perioperative nursing care for colon cancer was used in group B.Patients in both groups were observed for postoperative time for passage of gas by anus,time for first food intake,length of stay in hospital,fee for hospitalization as well as incidence of postoperative complications,and comparative analysis was carried out.Results Time for passage of gas by anus(t value was 3.571),time for first food intake (t value was 8.944),length of stay in hospital (t value was 3.397),fee for hospitalization (t value was 3.950) as well as incidence of postoperative complication in group A were lower than those of group B.The satisfaction degree of group A was 96.67％(29/30) while 83.33％(25/30) in group B and the satisfaction degree between the two groups was statistically significant.Conclusions In the primary hospital,the fast track surgery care used in colon cancer can not only speed up the recovery process of the patients,shorten hospital stay,reduce hospital cost and the incidence of postoperative complications,but also can reduce the workload of nurses and improve the satisfaction degree of patients.

Vitamin D adjusts the body′s absorption and utilization of calciumand phosphorus. Vitamin D deficiency is closely related to incidence of many disease. Several disorders,both systemic and those of the nerv-ous system,have been linked with vitamin D deficiency. Neurological disorders with a vitamin D link include but are not limited to multiple sclerosis,Alzheimer and Parkinson disease,as well as cerebrovascular disorders. In re-cent years,studies have found that the vitamin D may play a critical role in the pathogenesis of epilepsy and pos-sess certain anticonvulsant effects. This article summarized the role of vitamin D in the nervous system and epi-lepsy,the level of serum vitamin D and the correlation of the polymorphism of its receptor genes with epilepsy. The review also summarizes the interaction between antiepileptic drugs and vitamin D.

Objective Perioperative fluid therapy has always been the most basic and important measure to maintain the stability of patients'cyclic system.Colloid and crystalloid,as common perioperative fluid,play different roles in clinical application due to the different molecular sizes.In the recent years,besides their contribution to maintain the stability of cyclic system,scientists have begun to pay attention to the effect on coagulation,especially their comparison.This paper mainly discusses the study status of the influence on coagulation produced by different liquids.