Objective To investigate the influenee of the consistency of resin composite and insertion techniques on the homogeneity of the Class I restorations.Methods Standardized Class I cavities were prepared in polymethyl methyacrylate(PMMA) blocks and restored with three resin composites (Prodigy,Tetric EvoCeram and Tetric Ceram HB)using either a packing or an injection technique by six operators.Then the restorations were sectioned longitudinally and inspected for the presence of porosities and voids with microscope.The consistence of the three resins was tested using an area method.Results There is little porosity in original resin.After insertion,large numbers of porosities were observed in restorations,with Tetric EvoCeram presented much more porosities(1137.1±365.0 for packing and 566.1 ±206.4 for injection)than Prodigy(241.0±116.1,195.8±28.7)and Tetric Ceram HB(193.1±35.8,156.3±33.0).Tetric Ceram HB showed the highest consistency,followed by Tetric EvoCeram and Prodigy.No linear correlation was found between the consistency of the composite and the porosity of their restorations.For Tetric EvoCeram.the restorations inserted with packing showed significant more porosity than that with injection.Contrastively,the restorations of Prodigy or Tetric Ceram HB presented no apparent difference for the two filling techniques.Conclusions The porosity in restoration was primarily created during the insertion.There was no linear correlation between the consistency of the composite and the porosity of their restorations.The porosity of composite resin is material-brand dependent.The influence of filling techniques on the porosity of restoration is depending on the composite used.

Intracranial haemorrhage (ICH) in a patient with relapse of idiopathic thrombocytopaenic purpura (ITP) can be lethal. The site of haemorrhage, compounded by low platelets in this disease, makes its management extremely challenging, especially when a neurosurgical procedure is warranted. We report a case report of an unconventional way of increasing platelet counts in ITP rapidly in an emergency setting.

Animals ; Anticonvulsants ; pharmacology ; Drug Evaluation, Preclinical ; methods ; GABA-A Receptor Antagonists ; pharmacology ; Motor Activity ; drug effects ; Pentylenetetrazole ; pharmacology ; Valproic Acid ; pharmacology ; Zebrafish

The arachidonic acid (AA) metabolic pathway participates in various physiological processes as well as in the development of malignancies. We analyzed genomic alterations in AA metabolic enzymes in the Cancer Genome Atlas (TCGA) prostate cancer (PCa) dataset and found that the gene encoding soluble epoxide hydrolase (EPHX2) is frequently deleted in PCa. EPHX2 mRNA and protein expression in PCa was examined in multiple datasets by differential gene expression analysis and in a tissue microarray by immunohistochemistry. The expression data were analyzed in conjunction with clinicopathological variables. Both the mRNA and protein expression levels of EPHX2 were significantly decreased in tumors compared with normal prostate tissues and were inversely correlated with the Gleason grade and disease-free survival time. Furthermore, EPHX2 mRNA expression was significantly decreased in metastatic and recurrent PCa compared with localized and primary PCa, respectively. In addition, EPHX2 protein expression correlated negatively with Ki67 expression. In conclusion, EPHX2 deregulation is significantly correlated with the clinical characteristics of PCa progression and may serve as a prognostic marker for PCa.