OBJECTIVEThe assessment result of microcirculatory perfusion provides significant evidence for the detection of microcirculatory alterations in sepsis patients, and functional capillary density based on vessel segmentation can reflect microcirculatory perfusion effectively.

METHODSFirst, preprocessing comprise operations to improve the quality of images in order to maximize the contrast between background and vessels. Secondly, adaptive local thresholding is used for initial segmentation to get the general vessel region. Finally, post-processing technique reduces the image artifacts and links the vessel fragment, and then functional capillary density is calculated based on the segmentation results.

RESULTSSeveral sublingual microcirculatory data have been tested, and the agreement between the results by AVA and the proposed algorithm reached up to 92%.

CONCLUSIONSThe method can realize automated assessment of sublingual microcirculatory perfusion rapidly and accurately, and has strong clinical value.

Objective To explore the value of serum progesterone level in the early diagnosis of ectopie pregnancy. Method Serum progesterone levels were measured prospectively in 36 patients with ectapic pregnancy and 30 women with normal intrauterine pregnancy. Results The serum progesterone level in ectopic pregnancy [(5.12± 3.98) μg/L] was lower than that in normal intrauterine pregnancy [(25.23±6.81) μg/L]. Recommended 11 μg/L as the cutoff point of progesterone concentrations in diagnosis of ectopie pregnancy, the sensitivity rate was 91.7%(33/36), specificity rate was 93.3%(28/30),missed diagnosis rate was 8.3% (3/36) in the diagnosis of eetopie pregnancy. Conclusion The serum progesterone detection can be considered as an early diagnostic method of eetopie pregnancy because it can be measured easily and quickly.

Objective To develop a kind of system of simulation and training on ECG for studying ,training and assessing of biomedical engineer. Methods The simulation training system was designed based on PC. In the hardware system, ADuC848 SCM was adopted as the primary controller, QuickStart exploration platform was used to develop firmware, and intelligent assessing system was explored by C++ Builder. Results Biomedical Engineer could be trained, checked and evaluated by the system. Conclusion The system, combining practice and examination, pays attention to basic skill training and thus improves studying efficiency.

Objective To provide references for improving the quality of TCM decoction pieces through the analysis on sample inspection of TCM decoction pieces of Huguosi TCM Hospital of Beijing University of Chinese Medicine (hereinafter referred to as our hospital). Methods In our hospital from January 2014 to December 2015, TCM decoction pieces with problems in this sample inspection were categorized according to reasons. Results Quality problems found in the sample inspection were categorized into 3 types, i.e. collection and preparation (69.79%), storage (20.93%) and other problems (9.31%). Cutting in collection and preparation (13.95%), worming in storage (9.30%) and breaking/crumbing (6.98%) were the most obvious problems. Conclusion Strengthening the management of procurement acceptance, strict control of storage and storage conditions, and paying attention to the use of other aspects of management can improve the quality of TCM decoction pieces.

Objective:Objective To investigate the clinical effect of Misoprostol promoting cervical mature for before abortion . Method:8 patients of induced abortion operation were enrolled in our hospital from January 2013 to January 2012 , and were randomly divided into observation group and control group, each group had 124 cases, the observation group was given misoprostol which placed in cvaginal fornix preoperative , and control group without prompting cervical ripening drug. Observation of cervical dilatation, degree of pain, operation time and bleeding volume in two groups of patients. Result: The efficiency rate of observation group was 96. 8%, significantly higher than 82. 3% in the control group, the difference was statistically significant (P<0. 05);there were 100 cases of painless in the observation group , and 51 cases in control group, the degree of pain in the observation group were obviously improved than the control group , the difference was statistically significant (P<0. 05);,operation time of treatment group was shorter than the control group, the amount of bleeding surgery was significantly lower than the control group, the differences were statistically significant (P<0. 05). Conclusion:Misoprostol is given before induced abortion , can relax cervical effectively, shorten operation time, reduce the patients' pain, reduce the difficulty of operation, increase the safety of operation , is worth the clinical promotion.

Polymyositis (PM) and dermatomyositis (DM) are inflammatory myopathies and slowly advanced of unknown etiology that affect the skeletal muscles.With the advancement of the constant research on the disease and use of new technology,people have a penetrating realization about the pathogenesis. The disease is thought to be associated with autoimmune as well as nonimmune mechanism. By now, there is no overall summary on the newest advancement of the pathogenesis.We summarized the progress on the pathogenesis of the disease in order to make it more clear to physicians.

Zinc is essential for cell proliferation and differentiation,especially for the regulation of DNA synthesis and mitosis.On the molecular level,it is a structural constituent of a great number of proteins including enzymes of cellular signaling pathways and transcription factors.Zinc can modulate cellular signal recognition,second messenger metabolism,protein kinase and protein phosphatase activities.It may stimulate or inhibit activities of transcription factors,and it may as well be used as a second messenger involved in cell signaling regulation.

Objective To observe the change of sinus heart rate turbulence (HRT) and its relation to left ventricular systolic function (LVSF) in patients with acute coronary syndrome (ACS). Methods The 103 ACS patients and 62 healthy subjects were enrolled in this study, all of them received 24-hour Holter monitor. And the values of turbulence onset (TO), turbulence slope (TS)and heart rate variability (HRV) SDNN were calculated according the Holter records. The clinical information, left ventricular ejection fraction (LVEF) and left ventricular end diastolic diameter (LVEDD) were recorded. Then the ACS patients were divided into acute myocardial infarction (AMI)group and unable angina pectoris (UAP) group according to the cardiac enzymes and electrocardiogram. Results The value of TO was significantly higher in ACS group than in control group [(0.17±1.40)％ vs. (- 0.26±0.99)％, P＜0.05], and TS was significantly lower in ACS group than in control group [R-R interval:(0.88±2.51) ms vs. (2.60±2.76) ms, P＜0.01].There were significant differences in TS, LVEF and LVEDD between AMI group and UAP group (all P＜0.01), but there were no differences in HRV and TO between the two groups. TS was positively correlated with LVEF (r=0.21, P＜0.05), while TO was negatively correlated with LVEF (r=-0.26, P＜0.05) by Spearman correlation analysis. Conclusions HRT is significantly blunted in ACS patients and can evaluate cardiac autonomic function. It may be as a predictor of LVSF in ACS patients.

Objective To study the effect and mechanism of invigorating spleen, clearing heat and eliminating stasis drugs on QOUH of gastric mucosa and expression of EGF and EGFR in rat model of gastric ulcer. Methods The rat model of gastric ulcer was constructed by injection of acetic acid. The rats were randomly divided into model group, ranitidine group, invigorating spleen group, clearing heat group, eliminating stasis group, complex prescription group and normal control group. The ulcer index (UI), regenerate mucosal thickness, lamina muscularis mucosal width were observed by HE, and the level of EGF was detected by ELISA. The expression of EGFR in the mucosa around the ulcer was detected by SABC kit immunohistochemical method. Results Compared with model group, the regenerate mucosal thickness, lamina muscularis mucosae defect width and UI in invigoraing spleen, clearing heat, elimianting stasis and complex prescription groups were different significantly (P

Objective To explore the differences of blood biochemical indicators between normal and kidney Yang deficient rats administered orally different doses of white prepared lateral root of aconite.Method Eighty Sprague-Dawley(SD) male rats were divided randomly into normal group and kidney Yang deficient model group,forty rats in each group.After producing kidney Yang deficient model by injecting hydrocortisoni natrii succinas in intramuscular,normal and model rats were respectively and randomly divided into little,low,medium,great dose group and blank group,eight rats in each group.Those rats were administered orally with different concentrations of white prepared lateral root of aconite every day for two weeks.Twenty-four hours after the last oral administration,the blood were obtained and used for testing adrenocorticotrophic hormone(ACTH),cyclic adenosine monophosphate(cAMP),cyclic guanosine monophosphate(cGMP),glucose(GLU),cholesterol(CHO),alanine aminotransferase(ALT),urea nitrogen(BUN).Results Before taking medicine,model rats' cAMP/cGMP and ACTH were lower than normal rats',and they showed a trend of rise with adding dose.Before taking medicine,model rats' GLU was lower than normal rats'.With adding dose,model rats' GLU increased,however,normal rats' GLU reduced.When taken medium dose white prepared lateral root of aconite,model rats' CHO was higher than normal rats'.Taking medicine made both groups ALT rise,but normal rats' went up more quickly than model rats'.Model rats' BUN was higher than normal rats',and taking low dose or little dose medicine could lead to incline of reduction.Conclusion White prepared lateral root of aconite caused different blood biochemical effects on normal and kidney Yang deficient rats,and more remarkable toxicity effect on normal rats.