1.Significance of ARHI protein in adenocarcinoma of the uterine cervix
Chinese Journal of Postgraduates of Medicine 2011;34(24):1-3
ObjectiveTo detect the expression of ARHI protein in adenocarcinoma of the uterine cervix and normal uterine cervix tissue, and explore the pathogenesis of cervical cancer. MethodsThe expression of ARHI protein in 30 cases of adenocarcinoma of the uterine cervix tissue and 30 cases of normal uterine cervix tissue was detected by immunohistochemistry and analyzed the relationship between ARHI protein and adenocarcinoma of the uterine cervix. ResultsThe expression of ARHI protein in adenocarcinoma of the uterine cervix tissue[46.67%(14/30)]was lower than that in normal uterine cervix tissue[90.00%(27/30)],the difference was significant (P<0.05). There was intimate correlation between ARHI protein and histological grade, FIGO stage of adenocarcinoma of the uterine cervix tissue (P < 0.05), there was no correlation between ARHI protein and lymphatic metastasis (P>0.05). ConclusionsThe expression of ARHI protein in adenooarcinoma of the uterine cervix decreases which indicates the expression of ARHI protein take part in the carcinogenesis of adenocarcinoma of the uterine cervix. It can be used as a new diagnostic indicator of adenocarcinoma of the uterine cervix.
2.Application of responsibility system for holistic nursing in high-quality nursing service in the department of respiratory medicine
Chinese Journal of Practical Nursing 2013;(3):71-73
Objective To evaluate the application efficacy of responsibility system for holistic nursing system in high-quality nursing service in the department of respiratory medicine.Methods Department of respiratory medicine unit which was the quality nursing service demonstrated unit in our hospital was selected.The nursing responsibility-bound grouping system was first performed and the nurse-incharge would provide the patients with whole and continuous nursing service.Before and 6 months after the application,the nursing job satisfaction was conducted and the nursing grouping system satisfaction was also evaluated among nurses and doctors.Results Six months after the application of nursing responsibility-bound grouping system,the patient satisfaction with nursing job was raised obviously in the unit.Conclusions The application of responsibility-bound grouping system can not only promote the activity but also mobilize the enthusiasm of nurses,and then high-quality nursing service was resulted.So the service system was worthy of clinical application.
3.Expression of c-myc, c-myb and c-erbB-2 protein in breast cancer
Chinese Journal of Primary Medicine and Pharmacy 2013;20(5):641-643
Objective To investigate the roles of aberrant expression of c-myc,c-myb,and c-erbB-2 in the development of breast cancer and its clinical significance.Methods Immunohistochimical technique (S-P method)was used to detect the expression of c-myb,c-myc and c-erbB-2 protein in 150 cases of breast cancer and 30 cases of benign proliferative lesions.heir relation to clinical pathological parameters were analyzed.Results The protein expression levels of c-myc,c-myb,and c-erbB-2 in breast cancer were higher than those in benign proliferative lesions and the difference was significant(all P <0.05).Expression of c-myc protein had obvious relationship to histological grade,lymph nodes metastasis and pathological types(all P < 0.05).Expression of c-myb protein related significantly to histological grade (P < 0.05).c-erbB-2 was associated with the tumor size,grading,PR expression (all P < 0.05).The association among the three kinds of genetic proteins was observed.Conclusion Expressions of oncogenes c-myc,c-myb,and c-erbB-2 proteins are higher in breast cancer and lower in benign proliferative lesions.The difference is significant.They are related to the grading and some other prognostic factors such as tumor size,pathologic types,PR expression and lymph node metastasis.The results suggest that these oncogenes are activated in the development of breast cancer and provoke cancerous cells multiplication.Expressions of oncogenes c-myc,c-myb and c-erbB-2 mightbe considered to be the prognostic indicators of breast cancer.
4.Current research of Zevalin and Bexxar in the field of radioimmunotherapy
Fudan University Journal of Medical Sciences 2009;36(6):786-788
Radioimmunotherapy using monoclonal antibodies incorporated with radionuclide has been showed to be an effective agent for refractory non-Hodgkin's lymphoma (NHL). Many anti-CD20 antibodies labeled with radionuclide, such as 90Y-ibritumomab tiuxetan (Zevalin) and 131I-tositumomab tiuxetan (Bexxar), have been reported to be effective for the treatment of patients with relapsed or refractory low-grade, follicular, or transformed NHL. This review summarizes the current advance in clinical trials and studies of Zevalin and Bexxar for the treatment of NHL patients.
5.All-trans retinoic acid-induced nerve cell differentiation of rat bone marrow mesenchymal stem cells inhibits T lymphocyte proliferation
Chinese Journal of Tissue Engineering Research 2009;13(40):7889-7892
BACKGROUND:Several studies have demonstrated that bone marrow mesenchymal stem cells (BMSCs) inhibit the proliferation and activation of T lymphocytes, exerting a role of negative immunological regulation. OBJECTIVE: To induce rat BMSCs to differentiate into neuron-like cells in vitro using all-trans retinoic acid and to investigate the effects of differentiated cells on T lymphocyte proliferation.DESIGN, TIME AND SETTING: A cytological in vitro observation was performed in Guangdong Medical College in March 2008.MATERIALS: Two male 4-week-old Sprague Dawley rats were provided by the Laboratory Animal Center of Guangdong Medical College. Fresh healthy human blood was provided by the Department of Clinical Laboratory, Guangdong Medical College. METHODS: Rat BMSCs were isolated by adherent method and were passaged in vitro. Cells of passage 5 were pre-induced with Low glucose-dulbecco's modified eagle's medium (LG-DMEM) containing 0.3 mg/L all-trans retinoic acid and 10% fetal bovine serum. Twenty-four hours later, aforementioned LG-DMEM was discarded and LG-DMEM containing 0.6 mg/L all-trans retinoic acid was added to induce cells differentiation into neuron-like cells. Fresh healthy human blood was taken to prepare responding cells. Rat BMSCs, as stimulator cells, were included for one-way mixed lymphocyte reaction. Four groups were designated. Control group: 100 μL of responding cells at a concentration of 1×109/L; Experimental group 1:1×104 neuron-like cells + 100 μL of responding cells; Experimental group 2:1×105 neuron-like cells + 100 μL of responding cells; Experimental group 3:1×106 neuron-like cells + 100 μL of responding cells.MAIN OUTCOME MEASURES: Morphological observation of induce-differentiated cells, identification of nerve cells, and one-way mixed lymphocyte reaction results.RESULTS: Fifty minutes after addition of inducing medium, under the optical microscope, BMSCs exhibited a typical morphology of perikaryon. Three hours later, most of cells became into bipolar or multipolar neuron-like cell appearance with cell bodies and processes. Immunocytochemical staining results showed that majority of cells exhibited neuron specific enolase- and nestin-positive expression and glial fibrillary acidic protein-negative expression. Compared with the control group, radionuclide counts per minute were significantly reduced in each experimental group (P<0.05). There was significant difference in radionuclide counts per minute between experimental groups (P<0.05). With increasing BMSCs amount, the inhibitory effects on T lymphocyte proliferation were more evident.CONCLUSION: AII-trans retinoic acid can induce neuron-like cell differentiation of rat BMSCs in vitro. The neuron-like cells can inhibit human T lymphocyte proliferation in a dose-dependent manner.
6.The expression and significance of ARHI and COX-2 protein in tissues of the adenocarcinoma of the uterine cervix
Clinical Medicine of China 2012;28(3):241-244
Objective To study the role of ARHI protein and COX-2 protein and to explore their correlation in pathogenesis of adenocarcinoma of the uterine cervix.Methods The expression of ARHI and COX-2 protein were detected both in 40 cases of adenocarcinoma of the uterine cervix and in 30 cases of normal cervix by immunohistochemistry.Results The positive rate of the expression of ARHI protein in adenocarcinoma of the uterine cervix was 50.0% (20/40),which lower than that in normol cervix controls 90.0% (27/30),the difference was significant ( x2 =12.43,P < 0.05 ) ; The positive rate of the expression of COX-2 protein in adenocarcinoma of the uterine cervix was 82.5 % (33/40),which higher than that in normol cervix controls 0 (0/30),the difference was significant (x2 =46.16,P < 0.05 ) ;The positive expression rate of ARHI was negatively correlated with the clinical stage of cervical adenocarcinoma ( x2 =3.96,P < 0.05 ) disease and pathological grade (x2 =7.04,P < 0.05 ).COX-2 protein was positively correlated with pathological grade (x2 =7.04,P <0.05 ),but had no correlation with the clinical stage of cervical adenocarcinomacervical adenocarcinoma stage of diseas(P > 0.05) ;The expression of ARHI protein and COX-2 protein wasn't correlated with lymph nodeatic meatastasis(P > 0.05);The expression of ARHI protein had correlation with the expression of COX-2 protein (r =0.31,P < 0.05 ).Conclusion Inactivation of ARHI gene and the abnormal expression of COX-2 protein are involved in the origin of cervical adenocarcinoma,their synergistic reaction may play an important role in tumor origin of cervical adenocarcinoma.
7.Clinical value of combined detection of CA72-4,CA19-9 in gastric juice in diagnosis of gastric carcinoma
Chinese Journal of Primary Medicine and Pharmacy 2008;15(11):1828-1829
Objective To explore the possibility of united test of CA72-4, CA19-9 in gastric juice in diagnosis of gastric carcinoma.Methods The gastric juices were respectively obtained by liquid collector made by ourselves through gastreecope in study group(30 cases of gastric cancer) and control groups(32 cases of gastratrophia,30 cases of gastric ulcer,and 30 cases of chronic superficial gastritis).The gastric juice content of CA72-4, CA19-9 were determined synchronously with ES-300 auto-enzymoimmuno-analyzer.Results The gastric juice contents of CA72-4,CA19-9 were (83.54±143.70) U/ml and (47.05±29.64) U/ml, and the positive rates were 76.66% and 43.33% in study group,respectively,and all higher than those of control groups(P <0.01 ).The level of CA72-4 was high in two of 32 patients with gastratrophia,and one was diagnosed as gastric carcinoma after nine months follow-up.Conclusion The combined detection of CA72-4 and CA19-9 provide main evidence for the diagnosis of gastric cancer and diagnostric significance of advanced gastric cancer.
8.Research progress of in-stent magnetic-mediated hyperthermia for tumor treatment
Journal of International Oncology 2008;35(11):858-860
Possessing the unique feathers of high targeting and inducing the active immunity of patients,magnetic-mediated hyperthermia(MMH)has been regarded as a very promising cancer-treatment approach.Researches about in-stent MMH for esophagus cancel have been widely carried out with focusing on heating mechanism,feasibility studies and clinical trials.
9.Research progress in diagnosis of intensive care unit patients with invasive pulmonary aspergillosis
Chinese Journal of Postgraduates of Medicine 2016;(2):186-189
Invasive pulmonary aspergillosis is valued in intensive care unit (ICU) patients, but in ICU patients, the diagnosis standard of the European Organization for Research on the Treatment of Cancer/Mycosis Study Group (EORTC/MSG) could easily lead to misdiagnosis and missed diagnosis. It’s imaging manifestations are diverse: halo sign, nodules or micro nodules, nodular shadow, pulmonary consolidation, wedge-shaped consolidation, voids, air crescent sign, ground glass shadow, and so on, and with the relative characteristic features. The sensitivity and specificity of galactomannan detection in bronchoalveolar lavage fluid (BALF) are high for diagnosis of invasive pulmonary aspergillosis in ICU patients, but the significance of the cultivation of lower respiratory tract specimens is limited.
10.Research progress of functional magnetic resonance imaging in self-referential processing of depression
Chinese Journal of Behavioral Medicine and Brain Science 2015;24(11):1049-1052
Objective To review the research progress of functional magnetic resonance imaging in Self -referential Processing (SRP) of patients with Depression based on the papers published from 2004 to 2014 at abroad and domestic,with an attempted to explore the neuromechanism of depression and to provide reference for treatment of depression.Methods Major online database including PubMed,EBSCO,Ovid,Medline,Clinicalkey,Wan Fang,CNKI,Cqvip,and CBM date bases were searched in Octobor 2014.The key words we used are depressionor depressive disorder,Self-referential Processing or rumination orautobiographical memory or Self-face recognition, Functional Magnetic Resonance Inaging(tMRI)and so on.Results 28 studies were adopted in this study,findings from the study showed:①rumination,autobiographical memory (AM) retrieval,and self-face recognition(SFR) are SRP paradigms,the cortical midline structures (CMS)and default mode network (DMN) were involved in the SRP of depression.②Both medication and cognitive behavioral therapy could reduce the abnormal activation of CMS and DMN in patients with depression.Conclusioins ①Both CMS and DMN may be the neuromechanism of SRP of depression.②Both CMS and DMN may be used as objective indicators to evaluate the treatment response in patients with depression.