The event of “diagnosing mental disorder but actually not” violates patients′right of informed con-sent and refused to health , showing some problems of “diagnosing mental disorder but actually not” and mandatory admission process and psychiatric judicial authentication , and also have some ethical problems .Therefore, relevant departments should perfect the laws and regulation , establish a hospitalized psychiatric patients appeal , the appeal channels , at the same time , physicians should follow a correct understanding , respect patients rights , disinterest-ed, the ethical principles of self -supervision and also use constraints should be regulated .

OBJECTIVE:To evaluate the effect of temozolomide(TMZ)in combination with dexamethasone(DXM)on the proliferation of human gliocytoma U251 cells in vitro.METHODS:Human U251 cells were assigned to 1 of the 3 groups: TMZ(10,25,50,100,200,400?mol?L~(-1),respectively)alone or in combination with 40?mol?L~(-1)DXM(TMZ+DXM group)or control group(none drug).After treatment for 72 hours,the cell morphology,cell inhibition ratio,cell cycle and the apoptotic rate were detected.RESULTS:When TMZ concentration was greater than 100?mol?L~(-1)the cell inhibition ratio was higher in TMZ-treated group than in TMZ+DXM-treated group(P

There was an early view that the injured neurons and axons in central neural system couldn't regenerate after injury. However, it was found by recent researches that the injured neurons and axons can recover in different degrees after spinal cord injury of adult animals. In proper environment, some injured axons of central nervous system can regenerate slightly and form functional synaptic connections with target cells. Based on that, application of neurotrophic factors and cellular transplant therapy might be developed to treat spinal cord injury in the future.

ObjectiveTo observe the effect of early rehabilitation on patients with severe closed traumatic brain injury in intensive care unite (ICU).Methods118 patients with severe closed traumatic brain injury were divided randomly into the treatment group and control group with 59 cases in each group. The patients of control group recieved respiration rehabilitation, bedsore prevention and urinary system nursing; while the patients of treatment group added with motor and cognitive rehabilitation. Scores of Glasgow coma scale (GCS), Fugl-Mayer assessment (FMA), mini-mental status examination (MMSE), disability rating scale (DRS) and activities of daily living (ADL) of all patients were recorded before and after treatment.ResultsThe scores of GCS, FMA (upper limbs), ADL and DRS of the treatment group patients were significantly difference with that of the control group after treatment (P<0.05). The MMSE scores of two groups had no significantly difference after treatment (P>0.05).ConclusionEarly rehabilitation on the patients of severe closed traumatic brain injury in ICU can improve brain function and reduce the disability rating scale. The improvement of cognitive ability is not obviously in a shot period.

Poly ADP-ribose polymerase(PARP)inhibitor is a targeted biological agent for the treatment of ovarian cancer, which is mainly suitable for platinum-sensitive ovarian cancer patients. However, data from clinical trials showed that PARP inhibitors have certain therapeutic effect on platinum-resistant or platinum-refractory ovarian cancer patients. It may become a new hope for treatment of ovarian cancer patients in the future.

Chinese compound priscriptions of traditional herbs are widely used in the alternative therapy for all kinds of diseases It remains unclear how the compounds in different herbs interact each other and which exacting mechanism can complain their effects The components and pharmacological effects of the combination of Chinese herbs are not due to the simple mixture of single herb's The substance basis research of combination herbs is one of the important issue for understanding their pharmacological mechanism Here the recent progress on the substance basis research of combination Chinese traditional herbs is reviewed, including the combinative effects and quantity ratio relation study, herbs serum study, chemical compounds research, the methods and techniques in chemical components analysis, and application foreground of Chinese compound priscription

OBJECTIVE: The goal of this study was to study the mechanism of substance P (SP)-mediated the neural control of mast cell (MC) degranulation. METHOD: Bone marrow mast cells from mice were cultured with stem cell factor (SCF), IL-3 and IL-4 (group A) and SCF, IL-3 (group B) for four weeks. Then the cells were harvested and reserved for studies. Western Blot hybridization technique was used to detect the expression of FcεR I α and NK-1R on MCs from the two groups. Then such cells were activated with SP (0, 0. 01, 0. 10, 1. 00, 10. 00 µg/ml, respectively) for 30 min. The histamine released into the supernatant and stored in the protoplasm was quantified by enzyme linked immunosorbent assay (ELISA). And the percentage of histamine release was calculated as a percent of total histamine content. RESULT: The expressions of FcεR I α and NK-1R on these mast cells in group A were statistically higher than in group B (P<0. 05). The MCs from two groups can be actived when stimulated by SP, but the level of MC degranulation in group A was higher than group B (P<0. 05). CONCLUSION Neuropeptide may stimulate MC degranulation through immunological and non-immunological pathways. In summary, the current study provides us with better understanding of the mechanism of neuropeptide-controlled MC deranulation, and this should be helpful for the further research involved in the mechanism and treatmemt of airway hyper-reactivity.
Animals ; Bone Marrow Cells ; Cell Degranulation ; Cells, Cultured ; Culture Media ; chemistry ; Histamine ; metabolism ; Interleukin-3 ; pharmacology ; Interleukin-4 ; pharmacology ; Mast Cells ; cytology ; metabolism ; Mice ; Stem Cell Factor ; pharmacology ; Substance P ; pharmacology

The Toll like receptors(TLRs)are essential transmembrane signaling receptors of the innate immune system that alert the host to the presence of a microbial invader.The recent discovery of the TLR has rapidly expanded our knowledge of molecular events that initiate host pathogen interactions.These functional attributes of the cellular receptors provide insights into the nature of pattern recognition receptors that activate the human antimicrobial defense systems.The fundamental significance of the TLR in the generation of systemic inflammation and the pathogenesis of septic shock is reviewed. The potential clinical implications of therapeutic modulation of these recently characterized receptors of innate immunity are also discussed.

Objective To analyze the differentially expressed proteomics on T lymphocyte in mice immunized with 14‐3‐3 recom‐bined vaccine and injected with phosphate buffer solution (PBS) .Methods Mice immunized with 14‐3‐3 recombined vaccine were separated in the experiment group and those injected with PBS were selected in the control group .Two weeks after immunization with 14‐3‐3 recombined vaccine ,total protein of T lymphocyte isolated from the two groups was collected and separated by using the two dimensional electrophoresis(2‐DE) .The differentially expressed proteomics were compared .Results The 2‐DE map shown 11 differentially expressed protein plots ,including 7 protein plots spotted in the 2‐DE map of T lymphocyte from 14‐3‐3 recombined vaccine immunized‐mice and 4 protein plots spotted in the 2‐DE map of T lymphocyte from PBS injected‐mice .Conclusion There are differentially expressed proteomics of T lymphocyte in PBS injected‐mice and 14‐3‐3 recombined vaccine immunized‐mice ,which could contribute to clarifing the mechanism on protective immunity of 14‐3‐3 recombined vaccine and optimizing vaccine strategy for improving vaccine effect .