Objective:To discuss management and use for electrocardiogram (ECG) monitor during detection and repairing.Methods: To solve the problems in the process of management and use of electrocardiogram (ECG) monitor through detection and repairing of this equipment, and will discuss the management measures of ECG monitor. Furthermore, the effective quality control measures for ECG monitor will be raised.Results: Qualified rate of ECG monitor is 80.47%.It is useful to improve measure of quality control by PDCA.Conclusion: Quality control will improve the utilization rate of medical resources, and reduce investment costs of these resources.

Objective To study the gene expression patterns in human lung squamous cell carcinoma and adenocarcionma with cDNA chip.Methods The mRNA was extracted from cancer tissue and normal lung tissue,and then was labeled by Cy5-dCTP or Cy3-dCTP.The mixed probes were hybridized to cDNA chip containing 4096 genes and to study the gene expression patterns in lung squamous cell carcinoma and adenocarcionma.Results Seventeen genes were up-regulated and 19 genes were down-regulated in both lung squamous cell carcinoma and adenocarcinoma,the expression of 20 genes were remarkable higher in squamous cell carcinoma than that in adenocarcinoma and the expression of 14 genes were remarkable lower in squamous cell carcinoma than that in adenocarcinoma.Conclusion Many genes are possibly involved in the initiation and progression of human lung cancer;cDNA chip technique might be a useful method in screening lung cancer implicated genes.

Recently,with the recognition of blood supply and anatomy of the liver caudate lobe,the intervention therapies of hepatocellular carcinoma in caudate lobe were enriched.However,because of the complex anatomy,the treatment of hepatocellular carcinoma in caudate lobe is still controversial.The interventional methods for hepatocellular carcinoma in caudate lobe were mainly included transcatheter arterial chemoembolization (TACE),percutaneous ethanol injection (PEI),transcatheter arterial embolization (TAE),radioembolization,radiofrequency ablation (RFA) and endoscopic ultrasound-guided ethanol injection.The present statues and progresses of interventional therapy of hepatocellular carcinoma in caudate lobe were reviewed in this article.

Acupuncture Therapy ; Adult ; Alcoholism ; therapy ; Humans ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Peripheral Nervous System Diseases ; therapy

Objective To explore the effect of anshinone II A (Tan II A) on induction of apoptosis in human nasopharyngeal carcinoma CNE1 cells and one xpression of Bcl-2 gene and COX-2 promoter. Methods CNE1 cells were Cultured in vitro and treated with various concentrations of Tan II A. The cytotoxicity effect of Tan II A against CNE1 cells was evaluated by MTT assay. The cell cycle distribution, apoptotic rate and Bcl-2 expression were investigated by flow cytometry. COX-2 promoter activity was detected by the dual luciferase assay. Results Tan II A treatment inhibited CNE1 cell proliferation In a concentration- And time-dependent manner (P<0.01). Compared with the control group, Tan II A groups had significantly higher apoptosis rates (P<0.01), and the apoptosis rates increased with the increase of Tan II A concentration; the cells were blocked in the G2/M phase. Tan II A inhibited Bcl-2 protein expression in a concentration- dependent manner with in 6 h after Treatment (P<0.05). Bcl-2 expression was initially increased and then decreased with the increase of T an II A concentrations (P<0.05) 24 h after treatment. Activity of COX -2 promoter decreased with the increase of Tan II A concentration (P<0.01). Conclusion Tan II A can inhibit the growth and promote the apoptosis of CNE1 cells; inhibition of Bcl-2 expression and COX-2 activity might be one of the mechanisms for the Tan II A-induced CNE1 cell apoptosis.

QIU Fa-zu points out that medical creation is very urgent and importance should be attached to fundmental knowledge,frontier knowledge,connecting the theoretical konwledge with the phical kowledge,analyzing experiences and intertional interactions.Through analyzing these important thoughts of QIU Fa-zu,the passage draws the conclusion that these thoughts are valuble to China' s medical management and scientific policy.

BACKGROUND:No ideal drugs can be used in the prevention and treatment of aseptic loosening of artificial joints. Some researchs showed that erythromycin has strong inhibitory effects on periprosthetic osteolysis. Its antibacterial activity, however, limits its application in artificial joint loosening prevention. LY267108 is a new type of erythromycin derivatives, eliminates the antibacterial activities, and retains the anti-inflammatory activity.
OBJECTIVE:To evaluate inhibitory effect of LY267108 on nuclear factor kappa B during osteoclast activation.
METHODS:RANKL and macrophage colony-stimulating factor were added to RAW264.7 cellline of a mouse model induced by osteoclasts. Simultaneously, different concentrations of alendronate sodium, erythromycin and LY267108 were cocultured for 48 hours. The activity of nuclear factor kappa B and content of intracytoplasmic inhibitory subunit of nuclear factor kappa B alpha were measured by electrophoretic mobility shift assay and western blot assay.
RESULTS AND CONCLUSION:LY267108 has a strong inhibitory effect on nuclear factor kappa B. 10 mg/L LY267108, 25 mg/L erythromycin and 10 mg/L alendronate sodium had similar inhibitory effects on nuclear factor kappa B, which was obviously stronger than 10 mg/L erythromycin. However, 25 mg/L LY267108 had strongest inhibitory effects. No significant difference in intracytoplasmic inhibitory subunit of nuclear factor kappa B alpha levels was detected among 10 mg/L LY267108, 25 mg/L erythromycin and 10 mg/L alendronate sodium groups, but was stil apparently higher than 10 mg/L erythromycin group. Levels of intracytoplasmic inhibitory subunit of nuclear factor kappa B alpha were highest in the 25 mg/L LY267108 group. Results indicated that LY267108 in the process of osteoclast activation had stronger inhibitory effects on nuclear factor kappa B compared with erythromycin, and its safety was higher than alendronate sodium. Simultaneously, LY267108 did not have antimicrobial activity, and became a potential ideal drug for prevention and treatment of aseptic loosening of artificial joints. However, the inhibitory effects of LY267108 on the degradation of inhibitory subunit of nuclear factor kappa B alpha would be a mechanism of inhibiting the activation of nuclear factor kappa B.

Nrf2/Keap1/ARE is an important signaling pathway in maintaining the balance between peroxide and antioxidants. Once oxidative stress happened, Nrf2/Keap1/ARE would be ac-tivated to regulate the expression of downstream antioxidant pro-teins that could reduce the injury from oxidative stress and the level of oxidative stress. Recent researches show that Nrf2/Keap1/ARE signaling pathway is closely related to refractory re-spiratory diseases such as pulmonary fibrosis (PF), lung canc-er, chronic obstructive pulmonary disease (COPD), suggesting that Nrf2/Keap1/ARE signaling pathway may be a potential ther-apeutic target. This review focuses on the role of Nrf2/Keap1/ARE signaling pathway in refractory respiratory diseases in order to further understand the related mechanism and provide evidence for the diagnosis and treatment of refractory respiratory diseases.

Objective To explore the clinical application of mirabilite abdominal hot compression bag designed by ourselves for the moderately severe acute pancreatitis ( MSAP ) or severe acute pancreatitis ( SAP) .Methods Seventy MSAP and SAP patients were randomly divided into the observed and control groups according to the admission date.Each group included 35 patients.All the patients received the routine treatment.The patients in observed group received the treatment of compression action on abdomen by mirabilite abdominal continual hot compression bag designed by ourselves, while the traditional mirabilite hot compression action was used in the control group.The APACHEⅡscore and the levels of plasma CRP, IL-1, IL-6 and TNF-αof all patients on the 1st, 3rd, 6th, and 9th day after being admitted, the rate of complications including the intra-abdominal hypertension (IAH), pancreatic pseudocysts (PPC) and pulmonary infection, the recovery time of intestinal function and mean hospitalization time were all recorded and the therapeutic efficacy was compared between the two groups.Furthermore, the therapeutic effects of MSAP and SAP were compared between two groups.Results The APACHEⅡscore and the levels of plasma CRP, IL-1, IL-6 and TNF-αwere not statistically different between the two groups on the 1st day after hospitalization.The APACHEⅡscore and the levels of plasma CRP, IL-1 and TNF-αon the 3rd day in the observed group were significantly lower than those in the control group(P<0.05 or <0.01).The APACHEⅡ score and the levels of plasma CRP, IL-1 , IL-6 and TNF-αon the 6th day in the observed group were significantly lower, comparing to those in the control group.The levels of serum IL-1 and TNF-αin the observed groups were significantly lower than those in control group on the 9th day.All the differences above were statistically significant ( P<0.05 or <0.01).In the observed group, 4 cases (11.4%) had IAH and 7 cases (20.0%) developed other complications.The time of intestinal recovery and mean hospitalization was (4.9 ±0.8)d and (13.1 ±2.1)d, respectively, in the observed group.In the control group, 11 cases (31.4%) had IAH and 10 cases (28.6%) had other complications, and the time of intestinal recovery and mean hospitalization was (5.5 ±0.8) d and (15.4 ±3.9)d,respectively,.The rate of IAH, intestinal function recovery time and hospitalization time in the observed group were significantly less than those in control group (P<0.05 or <0.01).There was no statistical difference on other complications between the two groups.The intestinal function recovery time and hospitalization time of MSAPs in the observed group were significantly less than those in control group [(5.0 ± 0.8)d vs (5.5 ±0.8)d, P=0.03; (12.7 ±1.7)d vs (15.2 ±3.6)d, P=0.02].The APACHEⅡ score and the levels of plasma CRP, IL-1, IL-6 and TNF-αof SAPs were not statistically different between the two groups.Conclusions The application of the mirabilite abdominal continual hot compression bag could alleviate inflammatory response of MSAP and SAP, decrease the rate of IAH, improve the intestinal function recovery time,and shorten the hospitalization time.

Vesicants are the main agents used to fill chemical weapons, and chemical weapons abandoned by the Japa-nese Army in China.The mustard-lewisite mixture, which was developed for cold weather or high-altitude use due to its lower freezing point, is a special and important agent.The toxicology, emergency treatment and clinical management of mustard-lewisite mixture poisoning are introduced in this paper.