Objective To study the gene expression patterns in human lung squamous cell carcinoma and adenocarcionma with cDNA chip.Methods The mRNA was extracted from cancer tissue and normal lung tissue,and then was labeled by Cy5-dCTP or Cy3-dCTP.The mixed probes were hybridized to cDNA chip containing 4096 genes and to study the gene expression patterns in lung squamous cell carcinoma and adenocarcionma.Results Seventeen genes were up-regulated and 19 genes were down-regulated in both lung squamous cell carcinoma and adenocarcinoma,the expression of 20 genes were remarkable higher in squamous cell carcinoma than that in adenocarcinoma and the expression of 14 genes were remarkable lower in squamous cell carcinoma than that in adenocarcinoma.Conclusion Many genes are possibly involved in the initiation and progression of human lung cancer;cDNA chip technique might be a useful method in screening lung cancer implicated genes.

Objective:To discuss management and use for electrocardiogram (ECG) monitor during detection and repairing.Methods: To solve the problems in the process of management and use of electrocardiogram (ECG) monitor through detection and repairing of this equipment, and will discuss the management measures of ECG monitor. Furthermore, the effective quality control measures for ECG monitor will be raised.Results: Qualified rate of ECG monitor is 80.47%.It is useful to improve measure of quality control by PDCA.Conclusion: Quality control will improve the utilization rate of medical resources, and reduce investment costs of these resources.

Recently,with the recognition of blood supply and anatomy of the liver caudate lobe,the intervention therapies of hepatocellular carcinoma in caudate lobe were enriched.However,because of the complex anatomy,the treatment of hepatocellular carcinoma in caudate lobe is still controversial.The interventional methods for hepatocellular carcinoma in caudate lobe were mainly included transcatheter arterial chemoembolization (TACE),percutaneous ethanol injection (PEI),transcatheter arterial embolization (TAE),radioembolization,radiofrequency ablation (RFA) and endoscopic ultrasound-guided ethanol injection.The present statues and progresses of interventional therapy of hepatocellular carcinoma in caudate lobe were reviewed in this article.

Acupuncture Therapy ; Adult ; Alcoholism ; therapy ; Humans ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Peripheral Nervous System Diseases ; therapy

Vesicants are the main agents used to fill chemical weapons, and chemical weapons abandoned by the Japa-nese Army in China.The mustard-lewisite mixture, which was developed for cold weather or high-altitude use due to its lower freezing point, is a special and important agent.The toxicology, emergency treatment and clinical management of mustard-lewisite mixture poisoning are introduced in this paper.

Adolescent ; Adult ; Child ; Female ; Humans ; Male ; Middle Aged ; Moxibustion ; Musculoskeletal Manipulations ; Snoring ; therapy ; Young Adult

BACKGROUND: Drug therapy can improve the clinical symptoms and extend the gestational age through the maternal way to treat intrahepatic cholestasis of pregnancy (ICP), however, fetal hypoxia and meconium-stained amniotic fluid can not clearly improved. OBJECTIVE: To explore the effect of replacement of amniotic fluid that contains high concentrations of bile acids by artificial amniotic fluid through amniocentesis in the rat model of ICP. DESIGN, TIME AND SETTING: Random control animal experiments were completed in the Laboratory (first-degree laboratory) of Animal Experimental Center in Chongqing Medical University from March to September 2008. MATERIALS: Forty SD pregnant rats with 15 days of pregnancy were randomly divided into intracavitary injection of S-Adenosyl-L-methionine (SAMe) (IUS), intracavitary injection of sodium chloride (IUN), intravenous injection of SAMe (IVS) and blank control (IC) groups, with 10 rats in each group. METHODS: On day 15 of pregnancy, ICP rat models were induced by daily injection of estradiol benzoate. On day 17 of pregnancy, 1 mg SAMe and 0.3 mL sodium chloride were injected into amniotic cavity of in the IUS and IUN groups through amniocentasis. Meanwhile, 30 mg SAMe were injected though tail veins in IVS group. No treatment was performed in the IC group. MAIN OUTCOME MEASURES: The meconium-stained amniotic fluid rate and the still birth rate were calculated. Total bile acid (TBA) level in amniotic fluid was measured. In addition, the expression of hypoxia-inducible factor 1 ?(HIF-1?) mRNA in the placenta of rats were examined by immunohistochemistry and reverse transcription PCR at the days 20 of pregnancy. RESULTS: The meconium-stained amniotic fluid rate in the experimental group was lower than that of the IC group (P

Some human leucocyte antigens(HLA),espeually class Ⅱ genotypes such as HLA DQ/DR are closely linked to type 1A diabetes mellitus(T1ADM).But the genetic susceptibility of HLA DR/DQ are different between Caucasian and Asian populations.The aspartic acid at position 57 of DQ? can not confer resistance to T1ADM in Asians.DQ2 and DQ8 are the well-known susceptible genotypes in Caucasians while DQ4 and DQ9 are in Asians.An unexpected high risk of T1ADM is observed for the linkage disequilibrium between DRB1*0301/ DRB1*0401 and DQ2/DQ8 in both the populations.The DQB1*0302,DQA1*0501,DRB1*0401,*0402,*0407 are associated with T1ADM in Caucasians,but it is not the case in Asians.In contrast,DQB1*0401 shows a positive association to T1ADM in Asians,has a protective effect against T1ADM in Caucasians.Additionally DRB1*09 could be another susceptible gene in Asians.

Objective To study the chemical constituents in the mature fruit of Momordica Charantia. Methods M. charantia was extracted by alcohol and then extracted by chloroform; isolation and purification were carried out by silica gel. Compounds were identified and elucidated by spectral and chemical methods. Results Five compounds were obtained and identified as aglycone of momordicoside F_1 (Ⅰ), 5, 25-stigmastadien-3-ol (Ⅱ), aglycone of momordicoside Ⅰ (Ⅲ), charantin (Ⅳ), and ?-sitosterol (Ⅴ). Conclusion For the first time, compound Ⅰ is found as the novel natural product and compound Ⅱ is found in the fruit of M. charantia in China.

Objective To explore the effect of anshinone II A (Tan II A) on induction of apoptosis in human nasopharyngeal carcinoma CNE1 cells and one xpression of Bcl-2 gene and COX-2 promoter. Methods CNE1 cells were Cultured in vitro and treated with various concentrations of Tan II A. The cytotoxicity effect of Tan II A against CNE1 cells was evaluated by MTT assay. The cell cycle distribution, apoptotic rate and Bcl-2 expression were investigated by flow cytometry. COX-2 promoter activity was detected by the dual luciferase assay. Results Tan II A treatment inhibited CNE1 cell proliferation In a concentration- And time-dependent manner (P<0.01). Compared with the control group, Tan II A groups had significantly higher apoptosis rates (P<0.01), and the apoptosis rates increased with the increase of Tan II A concentration; the cells were blocked in the G2/M phase. Tan II A inhibited Bcl-2 protein expression in a concentration- dependent manner with in 6 h after Treatment (P<0.05). Bcl-2 expression was initially increased and then decreased with the increase of T an II A concentrations (P<0.05) 24 h after treatment. Activity of COX -2 promoter decreased with the increase of Tan II A concentration (P<0.01). Conclusion Tan II A can inhibit the growth and promote the apoptosis of CNE1 cells; inhibition of Bcl-2 expression and COX-2 activity might be one of the mechanisms for the Tan II A-induced CNE1 cell apoptosis.