Objective:To investigate the changes of the protein expression in heart tissue of rats after ischemia-reperfusion(I/R) injury and pre-treatment of carvedilol, and to elucidate the new protective mechanism of carvedilol pre-treatment to I/R heart injury. Methods:18 rats were randomly divided into sham-operation group, I/R injury group and carvedilol pre-treated group. The model of myocardial I/R injury was established by 30min ligation of the left anterior descending branch of the coronary artery and 2h reperfusion. Serum malondiadehyde (MDA) was determined, and the cytosolic fraction of myocardial tissue homogenate was separated applying two-dimensional gel electrophoresis technique. Differentially expressed protein spots were figured out with PDQuest software, while preliminary protein-identification was carried out by means of the two-dimensional gel electrophoresis database on the web. Results:Compared with the sham-operation group, after I/R injury, serum MDA was increased(P

OBJECTIVEThis study aimed to evaluate the effect of resin infiltration and fluoride solution on masking different demineralized white spot lesions by assessing color change.

METHODSArtificial white spot lesions were produced on 60 human molars. Each sample had at least two enamel surfaces opened (named A and B). The samples were randomly divided into groups 1, 2, and 3 according to their time of demineralization (24, 48, and 72 h). After demineralization, the A spot of each sample was treated by resin infiltration. The B spot was treated with 0.1% fluoride solution daily for 30 days. After the remineralization of the B spot, resin infiltration was used again on the B spot of each sample. Color assessment was performed by a spectrophotometer in five distinct stages: baseline, after the production of artificial caries, after resin infiltration of A spots, after 30 days of fluoride solution treatment of B spots, and after resin infiltration of remineralized B spots.

RESULTSBefore demineralization, the L* values of spots A and B in all groups were not significantly different (P > 0.05), whereas the L* values of spots A and B were significantly increased after demineralization. The L* values of A spots recovered significantly after treatment by resin infiltration (P<0.05), but only groups 1 and 2 reached the baseline. The L* values of B spots had no significant differences (P > 0.05) after fluoride treatment compared with that after demineralization. After resin infiltration on B spots, the L* values recovered but could not reach the baseline nor the level of A spots treated by resin infiltration only.

CONCLUSIONResin infiltration is a more effective treatment for masking white spot lesions than traditional fluoride treatment. The effect of masking white spot lesions has certain relationships with the degree of demineralization and activity of the lesion.

Objective To investigate the status quo and characteristics of medical students'job choosing view,to analyze the reasons and to propose countermeasures.Methods Questionnaires on job choosing view concerning job choosing cognition,ideals,method,motivation and values were conducted among 1700 medical students in Shandong University.Data entry and statistics were done by SPSS 17.0.Difference was analyzed by t-test and ANOVA.Results Significant differences were showed in sex,whether being the only-child in the family,father's education and family's economic status(P＜0.05).Conclusions Medical students'job choosing views are characterized by professional,highly expected,diversified and utilitarian,which is caused by society,school and family.Therefore,much importance should be attached to improving educational environment,making educational objective clear and constructing educational content system.

Objective To discuss incidence and related risk factors of vascular cognitive impairment (VCI) after acute subcortical infarction (SI).Methods One hundred and eighty-two patients with acute SI were divided into two groups:VCI group (81 cases) and no-VCI group ( 101 cases) according to VCI diagnostic standard.Sex proportion,age,culture degree between two groups were compared,and correlation was analyzed between the quantity of risk factors and the incidence of VCI.Results The incidence of VCI after acute SI was 44.5％(81/182).According to age stratification,the incidence of VCI in the patients of 56-65 years was 35.4％ (23/65),in the patients of 66-75 years was 45.3％ (48/106),and in the patients of 76-85 years was 90.9％ (10/11 ).The age in VCI group was significantly higher than that in no-VCI group [(69.93 ±6.91) years vs. (67.62 ±5.56) years,P =0.014],and culture degree was significantly lower than that in no-VCI group (P =0.028).The sex proportion between two groups had no significant difference (P =0.876).The more accompanying risk factors,the higher incidence of VCI.Conclusions Along with the increase of age,the incidence of VCI after acute SI is higher.The more accompanying risk factors,the higher incidence of VCI.

Objective To observe the effect of herbs of cleaning away heat,drying dampness and invigorating spleen on insulin resistance and TNF-? of the patients with early stage of type 2 diabetes mellitus.Method Eighty-eight patients with type 2 diabetes mellitus were randomly divided into 2 groups.Forty-four cases of the treatment group were treated with herbs of cleaning away heat,drying dampness and invigorating spleen,and 44 cases of the control group were treated with metformin.The data of their weight,glucose,insulin and TNF-? before and after treatment was compared.Results Glucose and insulin of two groups declined(P

Arterial embolization of thyroid gland is a new way to treat Graves' disease. However, there exist some problems about this treatment and no normalized procedure techniques have been stated. The authors reviewed many research papers with regards to the current status of this treatment and its existing problems.

Objective:To evaluate the alternation of homocysteine (Hcy),folic acid(FA),CA15-3 and vitaminB12(VitB12) in patients with breast cancer and explore the correlation of them.Methods:The levels of FA ,CA15-3 and VitB12 in serum of 55 breast cancer and 35 benign tumor of breast and 50 controls were measured by electro-chemiluminescence immunoassay ( ECLI ) , the Hcy levels were measured by colorimetric method.Results:Compared with the normal controls and benign tumor of breast cancer ,there were some significantly changes in breast cancer patients including Hcy ,CA15-3 increased and FA ,VitB12 reduced ( P＜0.05 ) ,there were no significant differences in Hcy,FA,CA15-3,VitB12 levels between benign breast tumors and normal controls (P>0.05).Companied with TNM stage increased ,the levels of FA breast cancer groups decreased ,the levels of Hcy,CA15-3 increased.The correlation analysis showed that Hcy was negatively correlated with FA and VitB 12,(r=-0.341,r=-0.540),CA15-3 was negatively correlated with FA, CA15-3 was postively correlated with Hcy (r=0.378,r=-0.361).Conclusion: Serum Hcy,CA15-3,FA and VitB12 detection in patients with breast cancer are helpful in the severity judgment and regimen prognosis .

Objective: To explore the Influence of ethics education on ethical beliefs of dual relationship of trainees who attend training of psychological consultation.Methods: Use of questionnaire,written and directed by "psychological counseling and treatment of ethical issues questionnaire(trainee attending psychological consultation training)" as a tool to evaluate,within the framework of Guangdong 368 trainees to investigate.Results: Compared with two groups of trainees,in refer to the ethical beliefs of dual relationship,the latter group who has a higher option of "not sure" shows varying degrees of decline in most of the items in the questionnaire.Two teams of trainees reach an identity of 80% or more(10 items in total) in the ethical judgment of dual relationship(choose "not").Two groups show significant differences in judging 5 items of the entries(p

According to the overview of historical development of ethical research in psycho-counseling and psycho-therapy in developed countries,this paper aims to summarize the research experience and outcome,in order to promote related Chinese ethical research based on China's realistic situation.

The localization of the ?-subunits of human chorionic gonadotropin(HCG)in 40 cases of colorectal carcinoma was investigated with PAP method.It was found that 9 cases out of the 40 were positive,in which 2 were Dukes' stage A,4 Dukes' stage B and 3 Dukes' stage C,accounting for 7.7%,44.4% and 60.0% of the total number of the corresponding Dukes' staging of this series.The positive cells were mainly situated in the infiltrating portions and distributed in clusters or diffusely.It is suggested that immunohistochemical study of HCG in colorectal carcinoma can be one of the indices in the monitoring of the malignant biological behaviors of colorectal carcinomas.