Objective:To investigate the changes of the protein expression in heart tissue of rats after ischemia-reperfusion(I/R) injury and pre-treatment of carvedilol, and to elucidate the new protective mechanism of carvedilol pre-treatment to I/R heart injury. Methods:18 rats were randomly divided into sham-operation group, I/R injury group and carvedilol pre-treated group. The model of myocardial I/R injury was established by 30min ligation of the left anterior descending branch of the coronary artery and 2h reperfusion. Serum malondiadehyde (MDA) was determined, and the cytosolic fraction of myocardial tissue homogenate was separated applying two-dimensional gel electrophoresis technique. Differentially expressed protein spots were figured out with PDQuest software, while preliminary protein-identification was carried out by means of the two-dimensional gel electrophoresis database on the web. Results:Compared with the sham-operation group, after I/R injury, serum MDA was increased(P

Objective To investigate the flora of pathogens and antibiotic resistance of urinary tract infection after spinal cord injury. Methods From June, 2014 to June, 2015, 145 inpatients with urinary tract infection after spinal cord injury were reviewed. Results The main pathogenic germs were found as E. coli (48.9%), K. pneumonia (19.7%) and P. mirabilis (9.0%), and they were resistant to the second or third generation cephalosporins and quinolones moderately or severely, but sensitive toβ-lactamase inhibitor combinations and aminogly-cosides. The strategy of antibiotics need more reasonable. Conclusion The main pathogens in urinary tract infection after spinal cord injury are Gram-negative bacilli, that are resistant to antibiotics. The antibiotics should be selected and used rationally according to bacterial culture.

It is a challenge in diagnosis of hemophagocytic lymphohistocytosis (HLH).Rapid diagnosis is the key to make decisions in clinical management of HLH.The timely and reliable diagnosis of HLH can be assisted by laboratory tests including nature killer (NK) cell activity, sCD25, hemophagocytosis, ferritin, surface CD107a, and genetic testing.Here is introduced lab assays and evaluate the clinical applications in HLH.The aim is to raise awareness of the values of laboratory evaluations in diagnosing HLH.

Objective To investigate the distribution and drug resistance of pathogen for pulmonary infection in cervical spinal cord inju-ry (CSCI) patients, and analyze the application of antibiotics. Methods From January, 2014 to June, 2015, 156 patients with CSCI were re-viewed. The bacteriology and drug resistance were investigated, as well as the application of antibiotics. Results There were 45 cases with nosocomial infection of 68 times, and 106 strains of bacteria in total. The top four Gram-negative pathogens were Klebsiella pneumonia (17.9%), Pseudomonas aeruginosae (15.1%), Encherichia coli (11.3%) and Acinetobacter baumannii (8.5%). The main Gram-positive cocci was Staphylococcus aureus (9.4%), and the main fungi was Candida tropicalis (0.7%). Gram-negative bacteria was resistant to the second or third generation cephalosporins and quinolones moderately or severely, but sensitive toβ-lactamase inhibitor combinations and aminoglyco-sides. The main Gram-positive cocci were sensitive to vancomycin. The application of antibiotics needed to be improved. Conclusion The Gram-negative bacteria are the chief pathogen for pulmonary infection in CSCI patients, which show multiple drug-resistant. It is significant to monitor the drug resistance of bacteria and optimize antibiotics.

Objective:To detect the effects of porcelain-fused-to-metal(PFM)crown on the periodontal tissue of the restored teeth,and the relationship between the levels of intedeukin(IL)-1β,aspartate transaminase(AST)and alkaline phosphatase(ALP)in gingival crevicular fluid(GCF)and the health condition of periodontal tissue.Methods:Among 28 patients,34 maxillary incisors with PFM crowns were selected for the study.There were 19 Ni-Cr PFM crowns and 15 Au-Pt PFM crowns.The contralateral homonymy natural teeth were healthy and treated as control teeth.The patients were followed up 6 to 8 months after restoring crowns.The values of IL-1β,AST and ALP in GCF were analysed.Results:The concentrations of IL1β and ALP were significantly higher in GCF of Ni-Cr PFM compared with those of control,whereas no significant difference in Au-Pt PFM group compared with that of control.The level of GCF-AST was higher in Ni-Cr PFM and Au-Pt PFM group than that of healthy control.Conclusion:Ni-Cr PFM crowns have negative influence on periodontal tissue,but Au-Pt PFM have little influence on periodontal tissue of the restored teeth.The activity of IL-1β,AST and ALP of GCF are sensitive to reflect the effect of PFM crowns of different materials on periodontal tissue.

Adoptive cell transfusion based on chimeric antigen receptor (CAR) is a new approach for treating malignant diseases and even advanced malignancies. More patients with advanced malignancies are expected to benefit from CAR T-cells once the suitable target molecules are selected, the safe method of gene transduction is applied, and the side effects of CAR T-cells are prevented.

Nrf2/Keap1/ARE is an important signaling pathway in maintaining the balance between peroxide and antioxidants. Once oxidative stress happened, Nrf2/Keap1/ARE would be ac-tivated to regulate the expression of downstream antioxidant pro-teins that could reduce the injury from oxidative stress and the level of oxidative stress. Recent researches show that Nrf2/Keap1/ARE signaling pathway is closely related to refractory re-spiratory diseases such as pulmonary fibrosis (PF), lung canc-er, chronic obstructive pulmonary disease (COPD), suggesting that Nrf2/Keap1/ARE signaling pathway may be a potential ther-apeutic target. This review focuses on the role of Nrf2/Keap1/ARE signaling pathway in refractory respiratory diseases in order to further understand the related mechanism and provide evidence for the diagnosis and treatment of refractory respiratory diseases.

Objective To discuss the application effect of TCM constitution recognition on TCM chronic disease refining health management for type 2 diabetes mellitus.Methods Totally 140 outpatients with type 2 diabetes mellitus from Chaoyangmen Community Health Center were selected and divided evenly into two groups after TCM constitution assessment. The study lasted for 2 years (1st August 2013–31st July 2015). The control group (70 cases) received diabetes community health management. In addition to routine community health management, TCM constitutional management was also administrated in the intervention group. The latter group received medicine, food, sport guidance according to the results of TCM constitution assessment. The blood glucose, blood lipid, BMI and waist-hip ratio of the two groups were monitored, and the self-management and life quality of patients were evaluated. Results After the intervention, significant improvement was found for fasting blood glucose, 2 h postprandial blood glucose, BMI, waist-hip ratio and blood lipid both in the control group and the intervention group, and the improvement in the intervention group was more obvious than the control group (P<0.05). The whole level of self-management and life quality was greatly increased in both groups, while improvement of the intervention group was much higher than the control group (P<0.05).Conclusion Integrated application of TCM constitutional theory in TCM chronic disease refining health management for type 2 diabetes mellitus will contribute to improving patients’ self-management, changing their clinical symptoms and finally improving their life quality.

The localization of the ?-subunits of human chorionic gonadotropin(HCG)in 40 cases of colorectal carcinoma was investigated with PAP method.It was found that 9 cases out of the 40 were positive,in which 2 were Dukes' stage A,4 Dukes' stage B and 3 Dukes' stage C,accounting for 7.7%,44.4% and 60.0% of the total number of the corresponding Dukes' staging of this series.The positive cells were mainly situated in the infiltrating portions and distributed in clusters or diffusely.It is suggested that immunohistochemical study of HCG in colorectal carcinoma can be one of the indices in the monitoring of the malignant biological behaviors of colorectal carcinomas.

Objective: To explore the Influence of ethics education on ethical beliefs of dual relationship of trainees who attend training of psychological consultation.Methods: Use of questionnaire,written and directed by "psychological counseling and treatment of ethical issues questionnaire(trainee attending psychological consultation training)" as a tool to evaluate,within the framework of Guangdong 368 trainees to investigate.Results: Compared with two groups of trainees,in refer to the ethical beliefs of dual relationship,the latter group who has a higher option of "not sure" shows varying degrees of decline in most of the items in the questionnaire.Two teams of trainees reach an identity of 80% or more(10 items in total) in the ethical judgment of dual relationship(choose "not").Two groups show significant differences in judging 5 items of the entries(p