Objective To discuss clinical factors associated with high serum bilirubin level postoperatively in patients with liver cancer. Methods In this study,130 postoperative cases of primary liver cancer were divided into two groups of high serum bilirubin and of normal bilirubin according to serum bilirubin level during two weeks of postoperation. Results Preoperative serum bilirubin、 Child grade、 operation time、 blood loss and types of hepatic vascular exclusion were correlated with the level of postoperative serum bilirubin. Types of hepatic vascular exclusion was a independent factor predicting postoperative elevation of serum bilirubin level. Conclusions Preoperative liver function, operation time, types of hepatic vascular exclusion and blood loss are all among factors influencing postoperative serum bilirubin level.

Objective To explore mimic antigen based ELISA for detecting serum anti-acquaporin4(AQP4) antibody in neuromyelitis optica(NMO) patients. Methods Three polypeptides: AQP456-69,AQP4135-155, AQP4209-230 were designed to simulate antigen epitopes of AQP4 through biological information and structure biology analysis, the peptides was used as antigen in ELISA to detect serum anti-AQP4 antibody in 9 NMO patients and 7 other miscellaneous neurological disorders which hayed been detected by immumofluorescence method. Results The mean value of A of anti-AQP4 antibody postive patients which have been determined by immumofluorescence method were higher than the controls in ELISA with AQP4135-155,AQP4209-230 as antigen (P<0.05). When the patients serum were diluted at 4 and 8 times, the A values were higher than controls significantly ( P<0.05 ). Conclusion Outmembrane polypeptides AQP4135-155,AQP4209-230 may be the main antigen epitope or main part of antigen epitope, they could be used to detect serum anti-AQP4 antibody in NMO patients.

Chronic Disease ; Humans ; Sinusitis ; therapy ; Treatment Outcome

OBJECTIVE:To compare the efficacy and safety of metoprolol and bisoprolol in the treatment of diastolic heart fail-ure. METHODS:150 patients with diastolic heart failure were randomly divided into group A(75 cases) and group B(75 cases). All patients received aspirin,angiotensin-converting enzyme inhibitors,angiotensin receptor antagonists,calcium antagonists,di-uretic and cardiac drugs and other conventional treatment;based on it,group A received Metoprolol tartrate tablet with initial dose of 6.25 mg,orally,twice a day,then increased to 100-200 mg based on improvement and tolerability;group B received 1.25 mg Metoprolol tartrate tablet,orally,once a day,then increased to 10 mg based on improvement and tolerability. The treatment course for 2 groups was 12 weeks. Clinical efficacy,and left ventricular end systolic diameter(LVESD),left ventricular ejection fraction (LVEF)and left ventricular end-diastolic diameter(LVEDD),myocardial performance index(LVTei index),brain natriuretic pep-tide(BNP),interleukin(IL)-1,IL-6,tumor necrosis factor(TNF)-α before and after treatment,and incidence of adverse reactions in 2 groups were observed. RESULTS:The efficacy in group B was significantly higher than group A,incidence of adverse reac-tions were significantly lower than group A,the differences were statistically significant (P<0.05). Before treatment,there were no significant differences in the LVESD,LVEF,LVEDD,LVTei index,BNP,IL-1,IL-6 and TNF-α in 2 groups(P>0.05). Af-ter treatment,LVESD,LVEDD,LVTei index,BNP,IL-1,IL-6 and TNF-α in 2 groups were significantly lower than before,and group B was lower than group A,LVEF was significantly higher than before,and group B was higher than group A,the differenc-es were statistically significant(P<0.05). CONCLUSIONS:Based on the conventional treatment,bisoprolol is significantly superi-or to metoprolol in terms of efficacy,relieving clinical symptoms and improving cardiac functions in the treatment of diastolic heart failure,with better safety.

Objective To investigate the relationship between antiendothelial cell antibodies(AECA)and anticardiolipin antibodies(ACA)in patients with lupus nephritis. Methods 58 sera from patients with lupus nephritis were studied. ELISA technique were used to detect both AECA and ACA, and immunoblotting was performed to determine specific endothelial targets. Results The prevalence of IgG-AECA and IgG-ACA positive were 36.2% and 39.7% respectively in the patients with lupus nephritis. 17 out of 23 patients with ACA had higher titers of AECA, while only 4 out of 35 patients without ACA were AECA positive(P

It is well known that oligosaccharides in glycoproteins and glycolipids play crucial roles in a variety of cellular functions, such as proliferation, differentiation, and intercellular communications. The oligosaccharides of cell are increasingly being recognized as one of the most prominent biochemical alterations associated with malignant transformation and tumorigenesis. Glycosylations in different cell cycle and cell growth periods are significantly distinct, and these differences affect the cell cycle progression. In the past decades, along with the advances in genomics and proteomics, the functional significance of cancer-associated changes in glycosylation has been revealed. Eukaryotic organisms depend on an intricate and evolutionary conserved cell cycle to control cell devision. Mistakes in cell cycle process lead to cancer. This review highlights the relations between cell cycle and glycosylation changes in cancer cells.

Aim To study the effect of echinacoside on behavior and proteins expression from substantia nigra and striatal tissue in MPTP mouse model of Parkinsons disease(PD)and discover the mechanism of its potential dopaminergic neuroprotective effect in the protein level.Methods The mouse model of PD was induced by 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)and the behavioral analysis of C57BL/6 mice was performed by using spontaneous movement and rotarod test.A proteomic approach based on 2-dimensional electrophoresis(2-DE),mass spectrometry(MS)and figure analysis was used to evaluate the effect of echinacoside on the behavior and the protein expression in substantia nigra and striatal tissue in C57BL/6 mice after MPTP administration.Results ① Compared with control,MPTP lesion significantly reduced the number of spontaneous movement and latent period of mice on the rotating rod(both P

OBJECTIVE： To explore and establish general testing methods for pigments in Chinese material medica, decoction pieces and Chinese patent medicine. METHODS： The approved supplementary testing methods and relevant literatures for pigments in Chinese material medica, decoction pieces and Chinese patent medicine were reviewed and summarized. Based on the practical experience and data, the general test methods were proposed and established by TLC, HPLC and LC-MS. The pigments were grouped according to color system. RESULTS： A large number of representative samples were used for the method validation. Fifteen pigments were detected in 923 batches of samples. CONCLUSION： The established general pigment test methods are feasible and could provide reference for the scientific supervision and the establishment of relevant standard method.

Objective To evaluate MRI in diagnosing brachial plexus preganglionic injury.Methods Twenty cases with brachial plexus preganglionic injury underwent M R scanning before operation.MR imaging was obtained by GE Signa EXCITE 1.5 T scanner.The scanning sequences included SE T1 WI,FSE T2 WI,T2 WI STIR and 3D Fast imaging employing steady state with phase cycled(3D-FIESTA-c).All the patients had exploration of the supraclavicular plexus and electrophysiology examination. And the accuracy, sensitivity and specificity of MRI in diagnosing preganglionic brachial plexus iniury were calculated with the standards of surgical and EMG results.Results Among the 73 pairs of i,jured roots.MR imaging detected the abnormalities in 63 pairs.The accuracy, sensitivity and specificity of MRI in diagnosing preganglionic brachial plexus injury were 86.5/(83/96),86.3/(63/73).87.0/(20/23),respectively.The direct signs of brachial plexus preganglionic injury included (1) lack or mutilation of netwe root in 54 pairs (85.7/),(2)coarsening,bending,stiff course and unable to be traced to the intervertebral foramen continuously in 9 pairs (14.3/).The indirect signs included (1)cystic cerebrospinal fluid gathering in the vertebral canal,posttraumatic spinal meningocele in 46 pairs (73.0/),(2)abnormal shape of nerve sleeve in 13 pairs(20.6/),(3)displacement and deformity of spinal cord in 50 pairs(79.4/),(4)abnormal signal of paravertebral muscles in 1 9 patients.Conclusion MRI can distinctly show the nerve rootlets within the vertebral canal,so it is helpful in making a correct diagnosis of brachial plexus preganglionic injuries.

Objective To observe the changes of calcitonin gene related peptide (CGRP) in rat spinal motoneuron after upper motoneuron injury. Methods Thirty six adult male Wistar rats were selected. Spinal cord transection was made at the lower thoracic segment. The lumbar regions of spinal cord were removed immediately, 1, 2, 4, 7 and 14 days after surgery to be divided into dorsal and ventral sections. The content of CGRP in ventral section was detected using radioimmunoassay method.Results The level of CGRP in spinal motoneuron of the lumbar region was decreased significantly after transection of spinal cord, reached the lowest level on the 2nd day after surgery and then maintained at the low level.Conclusions The upper motoneuron injury can result in the decrease of CGRP content in spinal motoneuron.