Objective:To investigate the changes of the protein expression in heart tissue of rats after ischemia-reperfusion(I/R) injury and pre-treatment of carvedilol, and to elucidate the new protective mechanism of carvedilol pre-treatment to I/R heart injury. Methods:18 rats were randomly divided into sham-operation group, I/R injury group and carvedilol pre-treated group. The model of myocardial I/R injury was established by 30min ligation of the left anterior descending branch of the coronary artery and 2h reperfusion. Serum malondiadehyde (MDA) was determined, and the cytosolic fraction of myocardial tissue homogenate was separated applying two-dimensional gel electrophoresis technique. Differentially expressed protein spots were figured out with PDQuest software, while preliminary protein-identification was carried out by means of the two-dimensional gel electrophoresis database on the web. Results:Compared with the sham-operation group, after I/R injury, serum MDA was increased(P

OBJECTIVEThis study aimed to evaluate the effect of resin infiltration and fluoride solution on masking different demineralized white spot lesions by assessing color change.

METHODSArtificial white spot lesions were produced on 60 human molars. Each sample had at least two enamel surfaces opened (named A and B). The samples were randomly divided into groups 1, 2, and 3 according to their time of demineralization (24, 48, and 72 h). After demineralization, the A spot of each sample was treated by resin infiltration. The B spot was treated with 0.1% fluoride solution daily for 30 days. After the remineralization of the B spot, resin infiltration was used again on the B spot of each sample. Color assessment was performed by a spectrophotometer in five distinct stages: baseline, after the production of artificial caries, after resin infiltration of A spots, after 30 days of fluoride solution treatment of B spots, and after resin infiltration of remineralized B spots.

RESULTSBefore demineralization, the L* values of spots A and B in all groups were not significantly different (P > 0.05), whereas the L* values of spots A and B were significantly increased after demineralization. The L* values of A spots recovered significantly after treatment by resin infiltration (P<0.05), but only groups 1 and 2 reached the baseline. The L* values of B spots had no significant differences (P > 0.05) after fluoride treatment compared with that after demineralization. After resin infiltration on B spots, the L* values recovered but could not reach the baseline nor the level of A spots treated by resin infiltration only.

CONCLUSIONResin infiltration is a more effective treatment for masking white spot lesions than traditional fluoride treatment. The effect of masking white spot lesions has certain relationships with the degree of demineralization and activity of the lesion.

Objective: To explore rational drug usage for periodontitis treatment through a comparative study of the efficacy of four kinds of drugs on the experimental periodontitis. Methods: 126 Wistar rats,10 randomly selected as normal control group, the other 116 were modeled by using local wire ligation and systemic prednisolone acetate injection. Successful animal models were randomly divided into model control group and berberine hydrochloride, minocycline hydrochloride, ginsenoside Rg-1 and transforming growth factor-β1 treatment groups, which were treated with each kind of medicine separately and were killed at the end of 1, 2, 4 weekend. Detected IL-1β, TNF-α, IL-6, BGP levels by immunohistochemical SABC assay. Results: IL-1β, TNF-α, IL-6 of the treatment groups were lower and BGP was higher than model control group(P<0.05).Among them, the role of berberine hydrochloride to reduce IL-1β and TNF-α was the best. The role of minocycline hydrochloride to reduce IL-6 was the fastest. The role of transforming growth factor-β1 to increase BGP was the fastest. The role of ginsenoside Rg-1 to increase BGP was more lasting and better follow-up. Berberine and ginsenoside Rg-1 reduced IL-1β, TNF-α, IL-6 and increased BGP at the same time. Conclusion: The treatment of minocycline hydrochloride and transforming growth factor-β1 are quick. Berberine and ginsenoside Rg-1, particularly berberine have the advantage of multi-targets' role.

Hepatocellular carcinoma(HCC) is the fifth most common cancer and the third cause of cancer-related death worldwide;meanwhile,it is also one of the fastest growing malignancies.The causes of HCC are multiple;HBV is the most important cause in China,with about 25%-30% of HBV infection patients finally develop hepatic cirrhosis and liver cancer.At present,large scale epidemiological studies revealed that the metabolic syndrome(MS) was closely related to liver cancer,and it even served as an independent risk factor for the occurrence and progression of liver cancer.Non-alcoholic fatty liver disease is a metabolic syndrome clinically manifested in the liver;recently it has been indicated to be closely related with HCC development and progression.This paper reviews the recent researches on metabolic syndrome and liver cancer,so as to provide literature for preventive and therapeutic studies on non-virus-related liver cancer.

Arterial embolization of thyroid gland is a new way to treat Graves' disease. However, there exist some problems about this treatment and no normalized procedure techniques have been stated. The authors reviewed many research papers with regards to the current status of this treatment and its existing problems.

Objective To study early face processing of internet addiction patients by using ERP method. Methods Ten healthy young man 18~21 years and 10 internet addiction patients 17~22 years served as subjects in experiment. Two runs of 300 stimuli (duration: 50 ms) of 3 facial and 3 non-facial pictures were randomly presented with equal probability (ISI: from 1 000 ms to 1 500 ms randomly), and the subjects were asked to react to facial stimuli and non-facial stimuli by pressing the left button and right button respectively as quickly as possible. Thirty two channel electroencephalogram (EEG) was recorded by Neuroscan Nuamps System. Results 1) Specific-face component Nd170 (face N170 minus object N170) was found in both groups, which was distributed at the temporal-occipital region in control groups but at occipital region in internet addiction group; 2) Compared with Nd170 in internet addiction group, Nd170 was significantly lower at occipital negion and delayed in control group. Conclusion The early face processing mechanism of internet addiction patients may be different from normal people.

Objective To observe the effect of herbs of cleaning away heat,drying dampness and invigorating spleen on insulin resistance and TNF-? of the patients with early stage of type 2 diabetes mellitus.Method Eighty-eight patients with type 2 diabetes mellitus were randomly divided into 2 groups.Forty-four cases of the treatment group were treated with herbs of cleaning away heat,drying dampness and invigorating spleen,and 44 cases of the control group were treated with metformin.The data of their weight,glucose,insulin and TNF-? before and after treatment was compared.Results Glucose and insulin of two groups declined(P

Objective:To evaluate the alternation of homocysteine (Hcy),folic acid(FA),CA15-3 and vitaminB12(VitB12) in patients with breast cancer and explore the correlation of them.Methods:The levels of FA ,CA15-3 and VitB12 in serum of 55 breast cancer and 35 benign tumor of breast and 50 controls were measured by electro-chemiluminescence immunoassay ( ECLI ) , the Hcy levels were measured by colorimetric method.Results:Compared with the normal controls and benign tumor of breast cancer ,there were some significantly changes in breast cancer patients including Hcy ,CA15-3 increased and FA ,VitB12 reduced ( P＜0.05 ) ,there were no significant differences in Hcy,FA,CA15-3,VitB12 levels between benign breast tumors and normal controls (P>0.05).Companied with TNM stage increased ,the levels of FA breast cancer groups decreased ,the levels of Hcy,CA15-3 increased.The correlation analysis showed that Hcy was negatively correlated with FA and VitB 12,(r=-0.341,r=-0.540),CA15-3 was negatively correlated with FA, CA15-3 was postively correlated with Hcy (r=0.378,r=-0.361).Conclusion: Serum Hcy,CA15-3,FA and VitB12 detection in patients with breast cancer are helpful in the severity judgment and regimen prognosis .

Objective To study the correlation between CT features and pulmonary function indexes in dif-ferent chronic obstructive pulmonary diseases(COPD).Methods Collection of COPD patients with 80 cases were collected,,the correlation analysis of COPD CT features (pixel index)and pulmonary function,including one second forced expiratory volume (FEV1 ),one second forced expiratory volume accounted for the percentage of forced vital capacity (FVC)and residual volume /lung total ratio (RV /TLC)were examined by pulmonary function testing (PFT)and multislice spiral CT (MSCT).Results According to CT,the regional area of lung density (LAA%)and airway wall thickness/lumen area were measured,COPD was divided into 56 cases of emphysema phenotype,13 case of airway phenotype and 11 case of mixed phenotype.The emphysema phenotype index,FEV1 ,FEV1 /FVC pixels,RV /TLC =27.68%,(32.19 ±16.78)%,(43.69 ±11.23),(55.67 ±15.49 ),airway phenotype were 5.23%, (55.67 ±23.12)%,(52.30 ±10.67),(49.48 ±13.27),respectively,mixed phenotype were 22.47% (41.23 ± 12.55)%,(47.31 ±9.98),(54.19 ±16.29),FEV1 ,FEV1 /FVC,pixel index,RV /TLC COPD CT in different phe-notypic differences were statistically significant (χ2 =19.013,F =6.234,7.854,6.389,all P <0.05).The emphyse-ma phenotype and airway phenotype in pixel index and FEV1 and FEV1 /FVC were negative correlation (r =-0.67,-0.94,-0.55,-0.42,all P <0.05),showed a positive correlation with RV /TLC (r =0.57,0.37,all P <0.05). Mixed pixel phenotype index and FEV1 ,FEV1 /FVC and RV /TLC were not significantly correlated (r =-0.56,-0.27,0.32,all P >0.05).Conclusion CT features can be regarded as the reference to diagnosis the COPD pheno-type and evaluate the effect of treatment,it is necessary to re -examine PFT in emphysema and airway phenotypes.

The localization of the ?-subunits of human chorionic gonadotropin(HCG)in 40 cases of colorectal carcinoma was investigated with PAP method.It was found that 9 cases out of the 40 were positive,in which 2 were Dukes' stage A,4 Dukes' stage B and 3 Dukes' stage C,accounting for 7.7%,44.4% and 60.0% of the total number of the corresponding Dukes' staging of this series.The positive cells were mainly situated in the infiltrating portions and distributed in clusters or diffusely.It is suggested that immunohistochemical study of HCG in colorectal carcinoma can be one of the indices in the monitoring of the malignant biological behaviors of colorectal carcinomas.