AIM:To investigate the effects of tanshinone IIA ( Tan) on cardiac action potential in rats with a-cute cerebral ischemia (ACI).METHODS:ACI was established in rats accordingly .Animals were divided into 3 groups:sham group, ACI group, and ACI with Tan treatment group .The defect of neural function in each group was graded , elec-trocardiogram was measured , monophasic action potential was recorded , and the levels of cardiac troponin I ( cTnI ) and creatine kinase-MB ( CK-MB) were examined by commercially available kits .RESULTS:Compared with ACI group , Tan significantly decreased the scores of the defect of neural function , reduced the incidence and duration of the abnormalities in electrocardiogram , prolonged the effective refractory period , action potential duration at 50%repolarization and action po-tential duration at 90%repolarization, and inhibited the elevation of cTnI and CK-MB levels.CONCLUSION:Tan main-tains a stable heart rhythm in ACI rats , which may be related to its protective effect on ischemic myocardium .

ObjectiveTo investigate the outcomes of mother a nd baby smaller than 37 gestational age caused by preeclampsia. Method sThe data for 122 preterm birth that was single pregnancy with preecla mpsia in our hospital from January 1993 to December 2002 were collected. The pri nciple of diagnosis and treatment, the outcomes were analyzed retrospectively. T he 122 pregnant women were divided into 3 groups according to their gestational age, more than 28 weeks and smaller than 31 +6 weeks was the first group (28 cases), more than 32 weeks and smaller than 33 +6 weeks was the second grou p (26 cases), more than 34 weeks and smaller than 36 +6 weeks was the thir d group (68 cases). The data were statisted with SAS software.Results The earlier the pregnancy-induced hypertension happened, the earlier the preeclampsia occured, the mean time was 14 03 d, 16 85 d,22 22 d, respect ively, (P0.05), the outcomes of mothers were good. Increasing the gestational age under the cond ition that the occurrene of comptications was not increased, 60 64% pregnant wo men accepted the treatment of improving the maturity of fetal lung. There were n o differences among the three groups at the morbidity of perinatal SGA, intracra nial hemorrhage (P 0.05) .ConclusionWe could improve the outcomes of pret erm birth caused by preeclampsia by enhancing the perinatal care, treating and t erminating pregnancy activey Increasing the gestational age, improving the matur ity of fetal lung would decrease the morbidity of severe RDS and improve the pe rinatal outcome effectively.

Regulatory T cells play an important role in regulating immune responses.Recently,a novel immune Treg cell with phenotype TCRαβ + CD3 + CD4-CD8-NK1.1-was known as the Double-negative T cells (DNT).DNT cells are able to suppress CD4 + and CD8 + T cells,B cells as well as NK cells and dendritic cells(DCs) and play an important role in regulating immune responses in models of autoimmunity,infection,inflammatory disease,transplantation and tumor immunity.Now DNT cells have already become a hot point in the research of the immunotherapy.This article mainly introduces the possible sources of DNT cells and the mechanisms of their functions.

Objective To study far femoral malignant bone tumor salvage limb treatment with allogeneic heterotopic half-joint transplantation and the use of GSH nail Methods Eleven cases with far femoral malignant bone tumor were administered tumor resection,transplanted with allogeneic heterotoeic bone half-joint,and fixed with GSH nail Results Nine out of the 11 cases were followed up.None of them had infection,rejection or heterotopic bone fracture.All of them can move with walking after 6 months.The effects of surgery was rated as excellent for 3 cases,fine for 4 cases andbad for 2 cases according to Mankin's rating criteria.Conclusion Salvage limb treatment for bone tumor can improve patients' quality of life.Allogeneic heterotopic bone,with rich source and acceptable joint surface,is a good graft for the treatment of bone defect after bone tumor resection.

Objective To observe the effects of posterior screw and rod fixation on nerve function and vertebral indicators in patients with lumbar vertebral fracture.Methods The clinical data of 32 cases with lumbar vertebral fracture were analyzed retrospectively.According to Frankle grading:grade A 1 case ;grade B 7 cases;grade C 12 cases ;grade D 12 cases.All the patients were treated by posterior screw and rod fixation.The nerve function and vertebral indicators were compared.Results Followed-up for 22.3 months,according to Frankle grading:grade A 1 case;grade B 3 cases;grade C 9 cases;grade D 15 cases;grade E 4 cases.The nerve functions were improved after operation.There was a significant difference between before and after treatment (all P ＜ 0.05).The the height of the front edge of the fractured vertebral and cobb angle were improved after operation (all P ＜ 0.01).Conclusion Posterior screw and rod fixation can significantly improve nerve function and vertebral indicators in patients with lumbar vertebral fracture.

Objective To investigate the value of interleukin-6 (IL-6) level as a prognostic indicator in diabetic ketoacidosis (DKA) patients.Methods Data of 88 cases of DKA in Sir Run Run Shaw Hospital collected from June 1,2010 to December 31,2012 were analyzed prospectively.They were divided into two groups according to the survival of patients followed up for 28 days:non-survival group (n =12) and survival group (n =76).Serum levels of IL-6,lactate,base excess and APACHE Ⅱ score in 24 hours after admission were compared between two groups by independent t test.The receiver operating characteristic (ROC) curve was plotted to analyze the performance of IL-6 as a prognostic indicator in the early stage of DKA.Correlations between IL-6 value and lactate,base excess,APACHE Ⅱ score were analyzed.Results The IL-6 values were elevated obviously in non-survival group.The ROC of IL-6 had an area under curve (AUC) of 0.783,and the cut-off value of IL-6 for the death followed up for 28 days was 448.9 ng/L.In all patients,IL-6 values and APACHE Ⅱ score showed a positive correlation between them (r =0.919,P =0.005),and also positive correlations existed between IL-6 and lactate as well as base excess (r=0.922,P=0.003; r=0.887,P=0.015)in24 hours.Conclusions IL-6 value is a reliable prognostic indicator in the early stage of DKA.

Hypogonadotropic hypogonadism (HH) is a heterogeneous group of diseases with variable clinical manifestations.Currently,the primary treatment of HH is hormone replacement therapy,including testosterone replacement for induction of pubertal development and gonadotropin therapy or pulse GnRH administration for induction of spermatogenesis.

Objective To compare the clinical curative effect of proximal femoral anatomic locking plate and DHS plate in treatment of femoral proximal fracture.Methods 86 cases of fracture of the proximal femur were randomly divided into the control group of 40 cases,46 cases in the observation group.The control group used DHS plate for treatment,observation group were treated with anatomical proximal femoral locking plate in the treatment of operation,operation time and the clinical efficacy of two groups were compared,the amount of bleeding; follow-up of 6 months,compared with two groups the quality of life of the patients.Results In the observation group,the excellent and good rate was 86.9%,which was better than 75% in the control group (x2 =4.307,P ＜ 0.05).The observation group operation time,operation bleeding volume were (106.3 ± 29.6) min,(171.6 ± 34.1) ml,were significantly better than the control group(149.7 ± 32.7) min,(329.7 ± 47.2) mL(t =6.459,7.569,all P ＜ 0.01).Two groups of patients with postoperative quality of life than the preoperative improve,observation group than in the control group was more obviously improved(t =4.417,P ＜ 0.05).Conclusion The anatomical proximal femoral locking plate is applied to treat proximal femoral fractures,which not only improves the effect of operation,but also improves quality of life.

Objective:To observe the clinical treatment effects of Tangkang Xifang in the patients with type 0 diabetes foot ( DF) . Methods:Totally 73 patients with type 0 DF were randomly divided into the treatment group (n=37) and the control group (n=36). All the patients discontinued other medications for 7d before the treatment. Base on the blood glucose control with the traditional treat-ment by metformin hydrochloride (1. 5 g·d-1), the treatment group was additionally given warm water foot bath with Tangkang Xi-fang, while the control group was additionally given warm water foot bath, 30 min per time and twice a day for 60 days. The changes in the clinical symptoms, ankle brachial index ( ABI) , motor nerve conduction velocity ( MCV) and some other indices were measured before and after the treatment. Results:In comparison with the control group (58. 3%), the treatment group had higher total effective rate (86. 5%), and the usage of Tangkang Xifang also significantly improved the physiopathologic syndromes of peripheral artery dis-ease and neuropathy (P<0. 01). The ABI and MCV in the treatment group were significantly better than those in the control group (P<0. 05 or P<0. 01). Conclusion:Tangkang Xifang can effectively improve the clinical symptoms of type 0 DF, which is suitable for the clinical application.

Objective; To evaluate the reliability and reasonability of maxillofacial skin color match using the digital camera. Methods; The stability of the experimental light source was evaluated before establishing the color measurement. 122 volunteers aged from 22 to 32 years were recorded by a Canon EOS 20D digital camera in studio environment when the light source was stable. Photographs were taken under a set exposure parameters(f/4.5,1/25 s,custom white balance,ISO 800) and saved as JPEG format. The CIE L~* a~* b~* color values were obtained from the digital images using Adobe Photoshop7.0 after color correction. All data were analyzed using SPSS 10. 0. Results;The experimental light source was stable in 21 to 70 minutes. It was suitable for color measurement during this period. The color parameters of all tested maxillofacial skin were:L~*49～66,a~*7 - 18,b~*5 -22. Conclusion;The assessment of maxillofacial skin color depend on method of measurement and instrument. The method of maxillofacial skin color matching by digital camera seems reliable.