AIM:To investigate the effects of tanshinone IIA ( Tan) on cardiac action potential in rats with a-cute cerebral ischemia (ACI).METHODS:ACI was established in rats accordingly .Animals were divided into 3 groups:sham group, ACI group, and ACI with Tan treatment group .The defect of neural function in each group was graded , elec-trocardiogram was measured , monophasic action potential was recorded , and the levels of cardiac troponin I ( cTnI ) and creatine kinase-MB ( CK-MB) were examined by commercially available kits .RESULTS:Compared with ACI group , Tan significantly decreased the scores of the defect of neural function , reduced the incidence and duration of the abnormalities in electrocardiogram , prolonged the effective refractory period , action potential duration at 50%repolarization and action po-tential duration at 90%repolarization, and inhibited the elevation of cTnI and CK-MB levels.CONCLUSION:Tan main-tains a stable heart rhythm in ACI rats , which may be related to its protective effect on ischemic myocardium .

ObjectiveTo investigate the outcomes of mother a nd baby smaller than 37 gestational age caused by preeclampsia. Method sThe data for 122 preterm birth that was single pregnancy with preecla mpsia in our hospital from January 1993 to December 2002 were collected. The pri nciple of diagnosis and treatment, the outcomes were analyzed retrospectively. T he 122 pregnant women were divided into 3 groups according to their gestational age, more than 28 weeks and smaller than 31 +6 weeks was the first group (28 cases), more than 32 weeks and smaller than 33 +6 weeks was the second grou p (26 cases), more than 34 weeks and smaller than 36 +6 weeks was the thir d group (68 cases). The data were statisted with SAS software.Results The earlier the pregnancy-induced hypertension happened, the earlier the preeclampsia occured, the mean time was 14 03 d, 16 85 d,22 22 d, respect ively, (P0.05), the outcomes of mothers were good. Increasing the gestational age under the cond ition that the occurrene of comptications was not increased, 60 64% pregnant wo men accepted the treatment of improving the maturity of fetal lung. There were n o differences among the three groups at the morbidity of perinatal SGA, intracra nial hemorrhage (P 0.05) .ConclusionWe could improve the outcomes of pret erm birth caused by preeclampsia by enhancing the perinatal care, treating and t erminating pregnancy activey Increasing the gestational age, improving the matur ity of fetal lung would decrease the morbidity of severe RDS and improve the pe rinatal outcome effectively.

Microorganism distributes in the organs of human body which connect with external environment, especially those organs in the gastrointestinal tracts, and it also plays a fundamental role in the physiopathology of the host's body. Because the microorganism is very small and has a great variety, it is difficult to reveal the significance of microorganism in the human physiopathology comprehensively and deeply. With the development of molecular biology, genomics, bioinformatics and other disciplines, the microbiome research will be more possible and easier. There are two key contents of microecology. One of these is to identify and quantify the diversity of microorganism, and the other is to reveal activity and the physiopathological function of microorganism in the host. Microbiome research methods, therefore, can be summarized as the traditional detection methods, construction of gene library, the genetic fingerprint analysis and molecular hybridization techniques and so on.
Computational Biology ; Gastrointestinal Microbiome ; Gastrointestinal Tract ; microbiology ; Humans

Inwardly rectifying potassium (Kir) channels exhibit an asymmetrical conductance at hyperpolarization (high conductance) compared to depolarization (low conductance). The KCNJ10 gene which encodes an inwardly rectifying K+ channel Kir4.1 subunit plays an essential role in the inner ear and hearing. Mutations or deficiency of KCNJ10 can cause hearing loss with epilepsy, ataxia, sensorineural deafness, and renal tubulopathy (EAST) or SeSAME (seizures, sensorineural deafness, ataxia, mental retardation, and electrolyte imbalance) syndromes. In this review, we mainly focus on the expression and function of Kir4.1 channels in the inner ear and mutation-induced EAST/SeSAME syndromes to provide insight for understanding the pathogenesis of deafness induced by KCNJ10 deficiency.
Deafness ; genetics ; metabolism ; Ear, Inner ; metabolism ; Hearing Loss, Sensorineural ; genetics ; metabolism ; Humans ; Intellectual Disability ; genetics ; metabolism ; Mutation ; Potassium Channels, Inwardly Rectifying ; genetics ; metabolism ; Seizures ; genetics ; metabolism

Objective; To evaluate the reliability and reasonability of maxillofacial skin color match using the digital camera. Methods; The stability of the experimental light source was evaluated before establishing the color measurement. 122 volunteers aged from 22 to 32 years were recorded by a Canon EOS 20D digital camera in studio environment when the light source was stable. Photographs were taken under a set exposure parameters(f/4.5,1/25 s,custom white balance,ISO 800) and saved as JPEG format. The CIE L~* a~* b~* color values were obtained from the digital images using Adobe Photoshop7.0 after color correction. All data were analyzed using SPSS 10. 0. Results;The experimental light source was stable in 21 to 70 minutes. It was suitable for color measurement during this period. The color parameters of all tested maxillofacial skin were:L~*49～66,a~*7 - 18,b~*5 -22. Conclusion;The assessment of maxillofacial skin color depend on method of measurement and instrument. The method of maxillofacial skin color matching by digital camera seems reliable.

Objective To study far femoral malignant bone tumor salvage limb treatment with allogeneic heterotopic half-joint transplantation and the use of GSH nail Methods Eleven cases with far femoral malignant bone tumor were administered tumor resection,transplanted with allogeneic heterotoeic bone half-joint,and fixed with GSH nail Results Nine out of the 11 cases were followed up.None of them had infection,rejection or heterotopic bone fracture.All of them can move with walking after 6 months.The effects of surgery was rated as excellent for 3 cases,fine for 4 cases andbad for 2 cases according to Mankin's rating criteria.Conclusion Salvage limb treatment for bone tumor can improve patients' quality of life.Allogeneic heterotopic bone,with rich source and acceptable joint surface,is a good graft for the treatment of bone defect after bone tumor resection.

Objective To compare the clinical curative effect of proximal femoral anatomic locking plate and DHS plate in treatment of femoral proximal fracture.Methods 86 cases of fracture of the proximal femur were randomly divided into the control group of 40 cases,46 cases in the observation group.The control group used DHS plate for treatment,observation group were treated with anatomical proximal femoral locking plate in the treatment of operation,operation time and the clinical efficacy of two groups were compared,the amount of bleeding; follow-up of 6 months,compared with two groups the quality of life of the patients.Results In the observation group,the excellent and good rate was 86.9%,which was better than 75% in the control group (x2 =4.307,P ＜ 0.05).The observation group operation time,operation bleeding volume were (106.3 ± 29.6) min,(171.6 ± 34.1) ml,were significantly better than the control group(149.7 ± 32.7) min,(329.7 ± 47.2) mL(t =6.459,7.569,all P ＜ 0.01).Two groups of patients with postoperative quality of life than the preoperative improve,observation group than in the control group was more obviously improved(t =4.417,P ＜ 0.05).Conclusion The anatomical proximal femoral locking plate is applied to treat proximal femoral fractures,which not only improves the effect of operation,but also improves quality of life.

Objective:To observe the clinical treatment effects of Tangkang Xifang in the patients with type 0 diabetes foot ( DF) . Methods:Totally 73 patients with type 0 DF were randomly divided into the treatment group (n=37) and the control group (n=36). All the patients discontinued other medications for 7d before the treatment. Base on the blood glucose control with the traditional treat-ment by metformin hydrochloride (1. 5 g·d-1), the treatment group was additionally given warm water foot bath with Tangkang Xi-fang, while the control group was additionally given warm water foot bath, 30 min per time and twice a day for 60 days. The changes in the clinical symptoms, ankle brachial index ( ABI) , motor nerve conduction velocity ( MCV) and some other indices were measured before and after the treatment. Results:In comparison with the control group (58. 3%), the treatment group had higher total effective rate (86. 5%), and the usage of Tangkang Xifang also significantly improved the physiopathologic syndromes of peripheral artery dis-ease and neuropathy (P<0. 01). The ABI and MCV in the treatment group were significantly better than those in the control group (P<0. 05 or P<0. 01). Conclusion:Tangkang Xifang can effectively improve the clinical symptoms of type 0 DF, which is suitable for the clinical application.

Objective To investigate the safety and efficacy of laparoscopic complete mesocolic excision (CME) with a medial-to-lateral approach for the treatment of right colonic cancer.Methods The clinical data of 46 patients with right colonic cancer who were admitted to the Longjiang Hospital from January 2010 to October 2013 were retrospectively analyzed.Twenty-four patients who received laparoscopic CME were in the laparoscopic group,and the other 22 patients who received open CME were in the control group.The intra-and postoperative condition and tumor recurrence of the 2 groups were compared.Patients were followed up till April 2014 after the operation.The measurement data were analyzed using the t test,and the count data were analyzed using the chi square test or Fisher exact probability.Results The operation was successfully done in the 2 groups.The operation time and intraoperative blood loss were (130 ± 26) minutes and (105 ± 29) mL in the laparoscopic group,and (156 ± 32)minutes and (136 ± 35)mL in the control group,with significant differences between the 2 groups (t =6.070,3.310,P＜0.05).The numbers of lymph nodes resected and lengths of resected specimen were 19 ± 4 and (28.0 ± 2.5)cm in the laparoscopic group,and 18 ± 4 and (26.8 ± 2.3)cm in the control group,with no significant differences between the 2 groups (t =0.560,1.770,P ＞0.05).The postoperative exhaust time and duration of postoperative hospital stay were (2.9 ±0.8)days and (12.3 ±2.7) days in the laparoscopic group,and (3.8 ±0.9)days and (14.1 ±2.2) days in the control group,with significant differences between the 2 groups (t =3.880,2.400,P ＜ 0.05).No operation-related complications was detected in the 2 groups.Forty-six patients were followed up for a mean time of 15 months (range,6-24 months).Two patients in the laparoscopic group and 2 in the control group were complicated with tumor local recurrence,with no significant difference between the 2 groups (P ＞ 0.05).No patients died during the follow-up.Conclusion Laparoscopic complete mesorectal excision with a medial-to-lateral approach for right colonic cancer is safe and feasible with satisfactory short-term outcome.