Objective To understand the main pathogenic bacteria distribution and drug susceptibility in the common bile duct calculi with infection ,so as to provide the basis for rational use of antimicrobial agents .Methods Data′s of 296 cases of bile specimens treated from January 2011 to December 2013 were retrospectively surveyed and drug resistance of bacteria culture results were analyzed .Results Among all the 296 cases ,there were 199 cases of pathogenic bacteria in bile specimens ,the positive rate was 67 .23% ;among them ,there were 21 cases of two kinds of bacteria ,a total of 220 strains of pathogenic bacteria were detected ,and among them there were 158 strains of gram negative bacteria ,accounting for 71 .82% ;there were 46 strains of gram positive bacte‐ria ,accounting for 20 .91% ;there were 16 strains of fungi ,accounting for 7 .27% ;the lowest resistance of gram negative bacilli lies in amikacin ,followed by tazobactam ,aztreonam ,three to four generations of cephalosporins and quinolone .Gram negative bacilli has the highest drug resistance to pseudomonas aeruginosa and e .coli ,and it has a drug resistant rate higher than 50 .00% to most anti‐microbial drugs .Gram positive cocci has 0 drug resistance rate to add ring element ,has a drug resistance rate lower than 30 .00% to vancomycin and rina thiazole amine ,and has highest drug resistance rate (70 .00% -100 .00% ) to erythromycin ,clindamycin and chlorine lincomycin .Conclusion Among all the 296 cases of common bile duct calculi bile with biliary tract infection ,the main path‐ogenic bacteria were e .coli ,klebsiella pneumoniae ,eosinophilic malt narrow and excrement enterococcus bacterium ;and the drug re‐sistance to penicillin is highest .

Objective To liver surgical site infection related influencing factors were discussed.Methods Retrospective analysis the clinical data of A total of 1 187 patients with liver and gallbladder surgery,using SPSS12.0 software for statistical analysis of data.Results A total of 1 187 cases of liver and gallbladder surgery patients and 63 cases of surgical site infection occurred,the in-fection rate of 5.31%,including incision shallow tissue 51 cases,8 incision deep tissue,organ lacuna in 4 cases,accounted for 80.95%,12.70% and 6.35% respectively.Isolated pathogenic bacteria distribution and composition ratios were as follow,15 strains of gram-negative bacteria(55.56%),12 gram positive bacteria strains(44.44%),including gram-negative bacteria in e.coli, gram-positive bacterium with staphylococcus aureus detection most(25.93% and 14.81% respectively),not isolated fungus;Age, presence of diabetes mellitus,drainage time,postoperative complications,operation time and open operation for independent risk fac-tors for surgical site infection (P <0.05).Conclusion Liver and gallbladder surgery in patients with surgical site infection is asso-ciated with a variety of factors,should strengthen the preoperative patients with postoperative condition evaluation,improve the lev-el of operation at the same time,thereby reducing surgical site infection.