The process of ATP biosynthesis with immobilized cells were reviewed from three respects: the active biological material of catalysis, the form of immobilized cells and the bioreactor of immobilized cells. The developing trend of ATP production was discussed and the suggestion to its research in future was also presented.

Objective To study the structural and anticorrosive property of microcrystalline α-Zn3 (PO4)2 ·4H2O. Methods Zinc phosphate was prepared from zinc acetate and orthophosphate acid in aqueous solution.Structural characteristics of products were investigated by XRD, RAMAN, FTIR, TG-DTA, SEM, surface area,particle size distribution, and density measurements. Results The title compound, a highly crystalline, micronized (A), c0 =5. 0304(A), V=975.86 (A)3. Its specific area is 0. 701 m2/g, density 3. 1612 g/m3 , and average size 4.75μm . Conclusion Comparing with commercial Zinc phosphate, the synthesized iamellar microcrystalline zinc phosphate had excellent anticorrosive property and dispersibility.

Objective:The best operation condition of extraction was found through analyzing the influences of both process of microwave pretreatment and hot wave washing. Methods: A new extraction method of chlorogenic acid was that the dry FLos Lonicerae was humidified with 75% ethanol-water solution at first, then the wet Flos Lonicerae was rapidly vaporized by microwave heating, at last chlorogenic acid was extracted after hot water washed twice, 10 minutes each time. Results: Compared with traditional extraction, the extraction time was about less than 6 times and then extraction ratio was increased by 1%. Conclusion: The method has high rate of extraction and short time of extraction.

AIM: The mechanism of extracting flavoinoids from Ginkgo biloba leaves by microwave prtreatment was studied systematically. METHODS: The dired leaves were used as the crude drug, the effect of the operation parameters during the extraction on the extraction result was analyzed with monofactor experiment, and spectrophotometer was used to observe the structure of the cells. RESULTS: After the microwave pretreatment, the structure of the leaves cell was destroyed and the effective composite was desorbed completely. The following extraction rate was 4 times higher that of traditional extraction, the yield and the purity were better than the ethanol extraction. CONCLUSION: The short extraction time, higher yield of effective composite, higher usage rate of the microwave, the method is worthy to be used. W

AIM:To extract pueraia isoflavone and soyisoflavones with n-butanol by means of inner ebullition from Radix puerariae lobatae.METHODS:A little ethanol was first used to saturate the powder of Radix puerariae lobatae to make the pueraia isoflavone desorbed adequately,and then hot n-Butanol solution was added to make the ethanol inside Radix puerariae lobatae begin to boil and the extraction was intensified.Based on different solubility of pueraia isoflavone and soyisoflavones in n-Butanol,when solvent vaporized,the soyisoflavones were separated and then the pueraia isoflavone by step vaporizeing on centrating crystal.RESULTS:80 mL of 60% ethanol,saturating time 20 min,600 mL n-Butanol including 20% water,at the temperature of 90 ℃,the yield of total isoflavone was 11.3 %.When 90% solvent vaporized,the ratio of quality of the soyisoflavones and pueraia isoflavone was 1∶3.The puerarin and daidzin's purity reached 46.0% and 6.12% by the HPLC respectively.CONCLUSION:The technology of inner ebullition extraction and solvent vaporizing crystal separation are simple,which provide industry production basis for pueraia isoflavone and soyisoflavones.