Objective:To study the expression of angiotensin-converting enzyme(ACE),extracellular signal-regulated kinase(ERK),and the changes of atrial fibrosis in patients with rheumatic heart disease(RHD) and atrial fibrillation(AF) treated with angiotensin-converting enzyme inhibitor(ACEI).Methods:Atrial tissues were obtained from the right appendage during open surgery in 35 patients with RHD.The mRNA of ACE and ERK2 were semi-qualified by reverse transcription-ploymerase chain reaction(RT-PCR) and normalized to the glyceraldehyde 3-phosphate dehydrogenase(GAPDH).Western blotting analysis was employed to examine the expressions of ACE and phosphorylated ERK(pERK).Atrial collagen volume fraction(CVF) was detected by Masson's stain.Results:The mRNA of ACE and ERK2 or the protein of ACE and pERK were significantly increased,and CVF was significantly increased in patients with chronic atrial fibrillation(CAF) compared with sinus rhythm group(SR)( P 0.05).Conclusion:The expressions of ACE,ERK2 and pERK increase,and fibrosis is more severe in RHD patients with CAF as compared with those with SR.Compared with CAF patients treated without ACEI,the expressions of ERK2 and pERK significantly decrease( P 0.05)in CAF patients treated with ACEI.This suggests that the increasing expression of ERK2 and pERK resulting from local renal angiotensin-converting enzyme system activation mediates the development of atrial fibrosis,and ACEI may contribute to lesser atrial fibrosis in RHD patients with AF.

According to the sera aquaporin-4 antibody (AQP4), neuromyelitis optica (NMO) can be classified into two types as AQP4 antibody positive (AQP4+) and negative (AQP4-). However, the NMO patients with AQP4- are prone to delayed treatment, and may have a different pathogenesis compared to that in patients with AQP4+. Scientific researches and the clinical trials on NMO with AQP4- will deepen the understanding of NMO pathogenesis and help to make an early accurate diagnosis and rational therapy for NMO with AQP4-. This review aims to summarize the progress in clinic diagnosis for NMO patients with AQP4-.

Pulmonary hypertension (PH) is a progressive disorder characterized by abnormally elevated blood pressure of the pulmonary circulation. PH progresses rapidly to right ventricular (RV) failure and even death without treatment. Cardiac magnetic resonance (CMR) is an accurate and reproducible tool for the assessment of RV morphology and function, which plays an important role in the prognosis of patients with PH. The aim of this study is to review the clinical application and research progress of CMR in evaluation of PH.

Apoptosis ; drug effects ; Asbestos ; toxicity ; Humans ; Mineral Fibers ; toxicity

Three different arrangements of neurology curriculum were compared including the traditional neurology teaching in Wuhan University School of Medicine,neurology teaching modules in clinical pathophysiology and therapeutics (CPPT) in University of Chicago Pritzker School of Medicine and the clinical pathophysiology and therapeutics (CPPT) as well as the current reform of neurology teaching in Wuhan University School of Medicine.This article discussed the issues related to the neurology curriculum integration in medical school.Results showed that curriculum integration in ‘ teaching reform class' was praised by teachers and students.Integrating basic medical courses into clinical courses of neurology is of great importances in improving the quality of medical education in China and cultivating high-quality medical personnel to meet the requirements of the times.

Pediatric knowledge was not fully learned by seven-year program medical students of pediatrics major in first five years,so two years of postgraduate was the key period for culturing their pediatric specialty.Students of seven-year program were characterized by strong theoretical knowledge but poor pediatric clinic knowledge,therefore,pediatric clinic teaching should emphasize on basic knowledge and skills,avoid dwelling on specific details at early stage,pay attention to their communicative ability with children and their relatives,combine theory with clinic practice and teach from surface to point.Meanwhile,we aimed at changing them into pediatric doctor through cultivating their clinic skills and scientific research ability.

This article disusses several ethical problems between patients and doctors from patients' entering into the hospital to the whole course of diagnosis and treat,meanwhile it brings forward some elementary countermeasures.In order to promote the development of orthopedical surgeons and orthopedics,it makes everybody familiar with ethical problems during the diagnosis and treat of orthopedical diseases

Objective To evaluate postoperative life quality of patients with chronic venous insufficiency(CVI). Methods According to the chronic venous insufficiency questionnaire(CIVIQ) of four sorts and twenty items, the life qualities of 169 postoperative patients receiving respectively three different surgery: super venous, perforator venous, and deep venous. The average life score of 80 is regarded as good. ResultsThe average score is 84?16 in super venous group、88?14 in perforator venous group and 82?16 in deep venous group. ConclusionsThe life quality of postoperative patients treated by deep venous valvuloplasty is satisfactory. CIVIQ is reliable and practicable for the life quality evaluation.

OBJECTIVE:To optimize the extraction process of Asiaticoside.METHODS:The extraction technique was optimized by single factor test and orthogonal design taking extraction rate of Asiaticoside as the indicator.RESULTS:The best extracting technical conditions were as follows:ultrasonically extracting 12 times amount of 60% ethanol for an hour.CONCLUSION:The extraction method is simple and feasible and by which high extraction rate of Asiaticoside was obtained.

OBJECTIVE:To observe the curative effect of Qingyan lifei mixture on cough with lung heat.METHODS:190 patients with cough with lung heat were randomly divided into two groups:104 cases in treatment group were assigned to receive Qingyan lifei mixtures 100 mL?time-1 bid,and 86 in control group to receive Qingfei yihuo tablets(4 tablets?time-1 bid).The course of treatment was 10 days for both groups.RESULTS:The total effective rate was 89.42% in treatment group versus 75.58% in control group,and the difference between the two groups was significant(P