Objective To analysis the hot spots of innovative technology of Chinese medicine industry in Shanghai during the recent years.Methods Patent Database of State Intelletual Property Office was retrieved by IPC class numbers combined with keywords and the IPC classification of the Chinese medicine patents in Shanghai area during 2002-2013 were under statistical analysis.Results The hot spot distribution of therapeutic activity of TCM preparations:Antineoplastic is the most important activity of Chinese medicine development, followed by the activities of treatment of inadequate blood flow or cardiovascular system diseases, such as hyperglycemia, high cholesterol, and so on. The activity of treatment of the digestive tract or digestive system diseases, followed by the activities of anti infection, antiviral, antibacterial, anti-inflammatory treatment, etc. The hot spot distribution of dosage:Researches on new dosage of Chinese medicine were mainly reflected in the improvement of traditional dosage form and development of new formulations. The capsules, tablets, oral liquid, and injections were the new dosage forms under the most researches. The distribution of medical resources:Medicinal plants or animals mainly came from “angiosperm”. Medicinal animals were less than medicinal plants, which mainly belonged to insecta.ConclusionThe hot spots of innovative technology of Chinese medicine industry in Shanghai are mainly distributed in the medicine of anti-tumor, cardiocerebral vascular diseases, digestive system diseases and so on in recent years. The researches of new formulation focus on the traditional dosage form improvement and new formulations development. “Angiosperm” is the key to the development of medicinal plants.

BACKGROUND:At present, the study to balance ability of functional ankle instability patients is less. The
balance ability deficiency may also be one of the important reasons for ankle repeated injury, so the research can effectively reduce the damage probability.
OBJECTIVE:To explore the static balance ability characteristics of functional ankle instability patients.
METHODS:Ten male subjects with unilateral functional ankle instability were enrol ed as the experimental group in the study, and other 10 healthy match-paired subjects were served as the control group. Their static balance ability was measured under double feet standing and single foot standing using win-pod system, the double feet testing lasted for 30 seconds and the single foot testing lasted for 10 seconds. Evaluation parameters of balance ability were obtained.
RESULTS AND CONCLUSION:During double feet testing, there were no significant differences in balance
ability parameters between the experimental group and the control group whether eyes be opened or closed (P>0.05), but the index values of double feet testing were obviously less than those of single foot testing. So we
concluded that in the double feet standing, there was no significant difference in the static balance ability between functional ankle instability patients and normal persons. Moreover, the static balance ability of double feet was
better than that of single feet. In the single foot standing of the affected foot, the static balance ability of functional ankle instability patients was slightly worse than that of the normal persons, especial y with eyes closed.
Comparison of the average swing between the X-axis and Y-axis found that in the process of maintaining balance posture, the swing direction of the body center of gravity was mainly in the anteroposterior direction.

BACKGROUND: The coating induced by conventional simulated body fluid (SBF) and 1.5-folds SBF as biomimetic method has the shortcomings of slowly growth with weeks or months growth cycle. OBJECTIVE: To evaluate Ti-6Al-4V alloy surface deposition of hydroxyapatite coating induced by 3-folds SBF. DESIGN, TIME AND SETTING: In vitro biological analysis. The experiment was performed at the Institute of Metal Research, Chinese Academy of Sciences from November 2008 to March 2009. MATERIALS: Ti-6A1-4V alloy was supported by Boda Metel Materials Co., Ltd. METHODS: The pretreated Ti-6Al-4V alloy was immersed in 37 'C 3-folds SBF. The SBF was replaced every 24 hours for 3 days. MAIN OUTCOME MEASURES: The morphology, phase and elemental composition of calcium phosphate coating were analyzed by scanning electron microscopy, X-ray diffraction and infrared spectroscopy analysis. RESULTS: The scanning electron microscopy exhibited that the surface of Ti-6A1 -4V alloy was covered with deposited film with granular crystal on local areas at day 1 after immersion. The deposited film covered surface of Ti-6A1-4V alloy at day 3 after immersion, and granular or scale-shaped crystal could be seen. The crystal deposition displayed sustained growth with certain area as center. The product comprised Ca, P, O, C elements without Al, V. X-ray diffraction and infrared spectroscopy analysis revealed that the main component of the coating was hydroxyapatite. CONCLUSION: The 3-folds SBF can accelerate the deposition as well as shorten formation duration of hydroxyapatite coating on Ti-6A1-4V alloy.

Objective To explore whether the inhibited expression of fibrocystin by RNA interference can increase epidermal growth factor (EGF)-induced cell proliferation and its possible mechanism . Methods A stable PKHD1-silenced HEK 293 cell line was established . Cell proliferation rate, intracellular Ca2+ concentration and extracellular signal-reguhted kinase 1/2(ERK1/2) activity were assessed after treatment with EGF, verapamil and Bay K8644 . Results The proliferation rate of PKHD1-silenced HEK-293 cells was found to be significantly higher after EGF stimulation compared to the control HEK 293 cell (231 .5% vs 152 .8%, P＜0 .01) . PKHD1-silencing lowered the intracellular Ca2+ concentration and caused EGF-induced ERK1/2 overactivation in the cells(P＜0 .01 ) . When cells were treated with verapamil for 4 hours to lower the intracellular Ca2+ concentration, the cell proliferation rate was significantly increased after 20 ng EGF for 24 hours . The verapamil treatment increased the level of activated ERK1/2 in EGF-treated cells . An increase of intracellular Ca2 + in PKHD1-silenced ceils repressed the EGF-dependent ERK1/2 activation and the hyperproliferative response to EGF stimulation . Conclusions Inhibition of fibrocystin can cause EGF-induced excessive proliferation through decreasing intracellular Ca2+ resulting in EGF-induced ERK1/2 activation . The loss of fibrocystin may lead to abnormal proliferation in kidney epithelial cells and cyst formation in ARPKD through modulation of intracellular Ca2+ concentration .

Objective To explore the changes of the levels of IL-8,LTB4 and its function in invasion in patients with chronic obstructive pulmonary disease(COPD).Methods The concentrations of IL-8 and LTB4 in serum and sputum were determined with enzyme linked immunosorbent assay(ELISA)in 30 cases of patients with COPD in acute aggravating stage and paracmasis and in 20 cases healthy adults.Blood routines were detected in all obstructs.Results The concentrations of IL-8 in serum and sputum in acute aggravating stage were(181.41±18.49)ng/L and(74.03±20.64)ng/L,significantly higher than those in paracmasis and the reference group(152.33±17.48)ng/L and(42.30±14.99)ng/L,P＜0.05).The concentrations of IL-8 in serum and sputum in paracmasis were significantly higher than those in reference group[(52.75±13.79)pg/ml and(17.15±5.36)ng/L,P＜0.05].The concentrations of LTB4 in sputum in acute aggravating stage were(2300.91±496.01)ng/L,significantly higher than those in paracmasis and the reference group[(1884.14±651.37)ng/L,P＜0.05].LTB4 level in acute aggravating is not significantly different from those in paracmasis and the reference group[(1941.30±301.62)ng/L and(1880.77±302.11)ng/L,P＞0.05).Conclusion Both IL-8 and LTB4 play important roles in the coupe of inflammatory reaction of air passage of COPD.

Post-stroke depression (PSD) is a common complication of stroke. Its patogenesis is complex, mainly including the mechanisms of neurobiotogy and social psychology. So far, there has been no uniform conclusion both at home and abroad. Now it is considered that PSD is the results of comprehensive effect of various neurobiology and social psychology, and its onset is in accordance with the bio-psycho-social medical model.

Aim To investigate the effect of fenofibrate on focal cerebral ischemia injury in rats and its mechanism.Methods The rat model of global cerebral ischemia/reperfusion injury was established by bilateral common carotid arteries occlusion combined with hemorrhagic hypotension.Fenofibrate (33, 100, 300 mg·kg~(-1)) was intragastriclly administered 30 min before the operation, MK886 (6 mg·kg~(-1)) was given intraperitoneally 30 min before administration of fenofibrate (300 mg·kg~(-1)).Morris water maze was used to evaluate the ability of spatial learning and memory function.HE staining was used to observe pathological morphological changes of hippocampal neurons. NF-κBp65 expression was detected by immunohistochemistry, SOD activities and MDA contents were analyzed by biochemistry, and IL-1β, IL-6, IL-10, TNF-α levels were detected by ELISA.Results Fenofibrate remarkably improved the spatial learning and memory function, obviously prevented the hippocampal neurons from karyopycnosis and losing induced by I/R. Fenofibrate significantly blunted the increase of MDA, NF-κBp65, TNF-α, IL-1β, IL-6, and the decrease of IL-10 and SOD activities of I/R rats.Conclusions Fenofibrate has an obviously neuroprotective effect on global cerebral ischemia/reperfusion damage by activating PPARα.The anti-inflammation and antioxidative stress of fenofibrate may be involved in the protective mechanism.

Objective To analyze the efficacy and safety of acupuncture treatment on eczema for providing the objective and reliable evaluation.Methods Cochrane systematic review methods were adopted.The author searched data in the VIP database(1987～2009),Qing Hua Tong Fang database(1997～2009),and China Academic Journals' Full-text Database(CNKJ)(1997～2009)concerned on acupuncture treatment of eczema and evaluated by RCT. Odds ratio(OR) and 95%confidence interval(95%C1)were used as the statistic efficacy for analysis.The quality of the papers was analyzed according to the four quality evaluation criteria in Cochrane Handbook and then meta-analysis(Meta)by RevMan4.2.2 software.Results There were 19 relevant Papers of randomized controlled trials(RCT)and non-randomized controlled trial (CCT)met the included criteria Meta analysis showed that there were statistically significant difference between acupuncture and conventional treatment of eczema.Conclusion Acupuncture treatment can improve the efficiency and reduce the recurrence rate of eczema.However,due to relatively low methodological trial adopted in these included papers,more scientific clinical trial designs were expected for provided high quality papers used for statistical analysis.

Tourette syndrome is a neuropsychiatric disorder characterized by the presence of both multiple motor and vocal tics. The pathogenesis is obscure . Infection and the immunologic abnormality after infection may be associated with this disease.The major study is about streptococcal infection. Interleukin may play an important role. Morever, some studies indicate that Mycoplasma pneumoniae infection is related to the Tourette syndrome.

Objective To investigate the prevention of intestines adhesion about sodium hyaluronic acid in postoperative intestines adhesion.Methods Eighteen cases of adhesive intestine obstruction were done intestinal release or bowel resection.2～4mL sodium hyaluronic acid was put to the wound,anastigmatic and rough surface of peritoneal.Gastrointestinal decompression,anti-infective and infusion were taken after oper-ations.Followed up 8～24 months.Results Obstructive symptoms haven't happened,the effective rate is 100%.Only 2 cases have intermittent abdominal pain without obstruction,the incidence was 11%.Conclu-sion Sodium hylauronic acid is effective to prevent the adhesion of postoperative intestines adhesion,simp-ler use,fewer side-effects and great value to appliance.