1.The development of military acute stress scale
Chinese Journal of Behavioral Medicine and Brain Science 2012;21(7):658-661
ObjectiveTo develop the military acute stress scale and examine the reliability and validity of this scale.MethodsBased upon the previous acute stress researches and the rule of psychometrics,the military acute stress scale was compiled according to open-ended inventory survey.A total of 630 personnel were chosen by random cluster sampling,and tested with this scale,50 of them were tested by Chinese Military psychosomatic health Scale simultaneously,and 50 of them were retested after two week.The reliability and validity were examined by exploratory factor analysis,confirmatory factor analysis and correlation analysis.ResultsThe military acute stress scale was comprised of 9 factors and one lie sub-scale ; Main fit indexes of model were the following CMIN =1219.476,DF =593,P =0.000,CMIN/DF =2.056,IFI =0.927,TLI =0.902,CFI =0.924,RMSEA =0.059; The conrelation coefficients between subscales and subscales were 0.284-0.572.The correlation coefficients between subscales and total scale were 0.700 ~ 0.846 (P < 0.05 or 0.01 ) ; The correlation coefficients was significant between this scale and Chinese military psychosomatic health scale.The correlation coefficients were 0.338-0.962 (P < 0.01 ).The test-retest correlation coefficients of total scale and subscales were 0.379-0.909 (P < 0.01 ).The Cronbaeh's α coefficients were 0.606-0.926 ; and the split-half correlation coefficients were 0.466-0.855.ConclusionThe military acute stress scale has acceptable psychometrics properties on reliability and validity.
2.Surface Enhanced Raman Scattering Analysis on Safflower Injections
China Pharmacist 2015;(5):759-760,761
Objective:To study the surface enhanced Raman scattering ( SERS) of safflower and identify safflower injections by SERS quickly and effectively. Methods:Through comparative analysis of the Raman spectroscopy of safflower injections and the corre-sponding control herbs, the rapid identification of safflower injections was realized. Results:The results showed that several character-istic peaks of safflower were enhanced obviously in SERS, which could be used to identify safflower injections. Conclusion:The meth-od is reliable, rapid, accurate and specific, which can be applied as a method to identify safflower and its injections.
3.Clinical laboratory tests of venous thromboembolism in obstetrics and gynecology
Chinese Journal of Laboratory Medicine 2017;40(3):149-151
Venous thrombotic disease is a serious disease,which impact on health and life-threatening.Pulmonary embolism and deep-vein thrombosis are the two components of a single disease called venous thromboembolism in obstetrics and gynecology.but it can be difficult to diagnose because clinical symptoms and signs are non-specific or absent in early venous thrombus embolism (VTE).It has great value that how to use the most economic,simple,efficient method for screened high-risk groups,timely and accurate laboratory diagnosis of VTE.
4.Analysis of lymphocyte sub-cluster in the patients with ovarian cancer around the chemotherapy
Chinese Journal of Postgraduates of Medicine 2011;34(21):12-14
Objective To detect the effects of chemotherapy on the immune function of patients with ovarian cancer.Methods Thirty-four patients with ovarian cancer(ovarian cancer group)who accepted chemotherapy were collected.Thirty healthy women were used as control group.The lymphocyte sub-cluster CD3+,CD4+,CD8+ and CD25+of peripheral blood were detected by flow cytometry.Results The peripheral blood CD3+,CD4+ and CD4+/CD8+ in ovarian cancer group were lower than those in control group(0.5524±0.1121 vs.0.6685±0.0869,0.3183±0.0864 vs.0.4139±0.0524,1.06±0.29 vs.1.43±0.21),CD4+ CD25+was higher than that in control group(0.1026±0.0418 vs.0.0287±0.0132),there was significant difference between two groups(P<0.05).There was no significant changes observed 1 week after chemotherapy(P>0.05).Three weeks after chemotherapy,the CD3+,CD4+ and CD4+/CD8+ in ovarian cancer group were higher and CD4+ CD25+ was lower,there were significant difference between two groups(P<0.05).Conclusions The ratio of lymphocyte sub-cluster is imbalance in the patients with ovarian cancer.Chemotherapy can improve the immunosuppression condition and recovery the ratio of lymphocyte sub-cluster in the patients with ovarian cancer.
5.Heparin-binding epidermal growth factor-like growth factor and necrotizing enterocolitis
International Journal of Pediatrics 2013;40(6):585-588
Necrotizing enterocolitis(NEC) is a common and devastating gastrointestinal disease that occurs predominantly in premature infants.Despite various advances in management,the mortality of this disease remains high.During the last decade,studies have shown that heparin-binding epidermal growth factor-like growth factor (HB-EGF),can protect intestinal epithelial cells from various forms of injury in vitro.Furthermore,studies using both an intestinal ischemia-reperfusion injury model in adult rats and neonatal rat model of NEC,have shown that HB-EGF can protect the intestines from injury.Present studies provide a new research direction in the prevention and treatment.
6.Effects of electro-acupuncture combined with hyperbaric oxygen on cognitive ability and plasma homocysteine in patients with dementia after cerebral infarction
Chinese Journal of Primary Medicine and Pharmacy 2013;20(21):3223-3225
Objective To explore the influence of electro-acupuncture combined with hyperbaric oxygen on cognitive ability and plasma homocysteine(Hcy) in patients with dementia after cerebral infarction.Methods 68 patients with dementia after cerebral infarction were randomly divided into the control group (34 cases) and the observation group(34 cases).The patients in the control group were treated through conventional treatment,and the patients in the observation group were treated through electric stimulation plus hyperbaric oxygen,one time every day,15 times was a course of treatment.The intelligence was evaluated and the level of Hcy was detected after treatment.Results Before treatment,the MMSE score and the plasma Hcy level of the observation group was (19.01 ±6.92) points,(26.84 ± 9.06) μmol/L,respectively,those of the control group was (18.76 ± 6.71) points,(26.14 ±8.79) μmol/L,respectively.After treatment,the MMSE score and the plasma Hcy level of the observation group was (25.77 ± 8.21) points,(15.15 ± 5.24) μmol/L,respectively,those of the control group was (20.18 ± 7.52) points,(21.32 ± 6.53) μmol/L,respectively.After treatment,the MMSE score of the observation group was significantly increased (t =3.6710,P < 0.05),and there was no significant change in the control group (t =0.5472,P > 0.05).The plasma Hcy level was significantly reduced after treatment in both two groups (t =2.0345,P < 0.01,t =2.5667,P <0.05),and that in the observation group was siginificantly lower than the control group (t =4.2970,P < 0.05).Conclusion Electric stimulation combined with hyperbaric oxygen can improve cognitive ability and decrease plasma Hcy level in patients with dementia after cerebral infarction.
7.Clinical study for dose-effect relationship of sufentanil with subarachnoid block during labor
Chinese Journal of Postgraduates of Medicine 2011;34(12):20-23
Objective To observe the effect and safety of different doses of sufentanil plus subarachnoid block during labor. Methods One hundred parturients (single birth) were divided into 5groups by random digits table with 20 cases in each, which sufentanil 3,4,5,6,7 μ g in group A, B, C, D, E plus ropivacaine 3 mg were injected respectively into subarachnoid space. The onset and duration of subarachnoid analgesia were observed; visual analogue scale (VAS) were recorded respectively before injection and 5,15,30,60 min after injection; the vital sign,labor stage,labor type,adverse reactions of parturients,fetal heart rate (FHR) and the Apgar score at 1,5 min of neonate were recorded respectively.Results The onset of subarachnoid analgesia in group A was higher than that in group B,C, D,E;the duration of subarachnoid analgesia in group A,B was shorter than that in group C,D,E.VAS declined gradually 5,15,30,60 min after injection compared with before injection in 5 groups (P < 0.01 );VAS in group B, C, D, E was lower than that in group A at 5 min after injection (P < 0.05 ), and VAS in group C, D, E was lower than that in group A,B at 60 min after injection (P< 0.05). There was no significant difference in vital sign,labor stage,labor type,FHR and the Apgar score of neonate among 5 groups (P >0.05). The occurrence of itch of skin and nausea in group D,E [25%(5/20), 15%(3/20) in group D,and 40%(8/20),20%(4/20) in group E] was higher than that in group A, B, C [5%(1/20), 0 in group A, 10%(2/20), 0 in group B, 10% (2/20), 0 in group C] (P < 0.05 ). Conclusion Subarachnoid block (sufentanil 4-6 μ g +ropivacaine 3 mg) can offer safety and efficacy of labor analgesia; sufentanil 5 μg is the best dose with subarachnoid block during labor at present.
8.Humanities quality education in college English teaching at medical colleges and universities
Chinese Journal of Medical Education Research 2013;(10):1075-1077
Based on the analysis of the medical students' humanities quality and current college English teaching,this paper explored four strategies including pre-class lead-in activity,making full use of current teaching materials,reforming present college English teaching method and developing extracur-ricular practical activities in order to foster humanities quality in college English teaching and to improve medical students' humanities quality.
9.Effect of Fructus Ligustri Lucidi on calcium metabolism and vitamin D-dependent gene expressions in ovariectomized rats
Chinese Traditional and Herbal Drugs 1994;0(04):-
Objective To investigate the effect of Fructus Ligustri Lucidi(FLL),a kidney-tonifying Chinese herbal medicine,on calcium metabolism and vitamin D-dependent gene expression in ovariectomized(OVX) rats.Methods Four weeks after OVX surgical operation the OVX rats were ig admini-(stered) with estradiol and FLL for 14 weeks.Ca~(2+) Level in serum,urine,and feces was measured by colorimetric methods,and gene expressions in duodenum and kidney were analyzed by reversed transcriptase-polymerase chain reaction(RT-PCR).Results Ovariectomy of rat led to significant loss of calcium in vivo,as demonstrated by decreasing serum Ca~(2+) level and increasing Ca~(2+) excretion rate in urine and feces in OVX rat group.Treatment of OVX rats with FLL could effectively restore serum Ca~(2+) level and prevent the increase of Ca~(2+) excretion from urine and feces.RT-PCR Analysis showed that both estradiol and FLL could prevent OVX-induced decrease of mRNA expression in duodenal vitamin D receptor(VDR).In kidney,FLL did not alter mRNA expressions of 25-hydroxyvitamin D 1-hydroxylase(1-OHase) and 25-hydroxyvitamin D 24-hydroxylase(24-OHase),while increased the mRNA expression levels of renal cal-ciumbinding protein-9k(CaBP-9k) and calcium-binding protein-28k(CaBP-28k).Conclusion FLL could improve the state of calcium imbalance induced by estrogen deficiency and the potential mechanism that contributes to the improvement of calcium status by FLL might involve the increase in intestinal sensitivity to active vitamin D through its up-regulation of VDR expression in duodenum and the enhancement in Ca~(2+) reabsorption via the up-regulation of calcium binding proteins expression in kidney.
10.Expression characteristics of 5 drug-resistance genes in 6 common adult malignant tumors
Journal of Chongqing Medical University 2007;0(10):-
Objective:To investigate the expression of five drug resistance genes in hepato cellular cancer,lung cancer,stomach cancer,colorectal cancer,ovarian cancer,breast cancer tissue. Methods:The expression of P-gp,MRP,LRP,GST-?,Topo-Ⅱ in 120 cases of malignant tumors were examined by immunohistochemistry stain. Results: 1. The expression of P-gp,MRP,LRP,GST-? in the 6 common cancers were relatively high,while Topo-Ⅱ was relatively low. 2. P-gp,MRP,LRP,GST-?,Topo-Ⅱ were all high expressed in hepatocellular cancer. 3. The coexpression rate of two and more genes was 95.83%,the coexpression rate of five genes was 21.67%. Conclusion:P-gp,MRP,LRP,GST-?,Topo-Ⅱ are associated with multidrug resistance in different degree,which can be regarded as valuable indicators for the decision of individual chemotherapeutic program.