The methods for analysis of cosmetics including chemical analysis and instrument analysis, such as spectrophotometry, electrochemical analysis, chromatography (gas chromatography, liquid chromatography and thin-layer chromatography), fluorescence analysis, were summarized in this article in recent years. The prohibited and limited components in cosmetics were determined mainly by chromatography method. The methods for determination of hygienic chemical indexes of cosmetics had been mature. The methods for determination of prohibited and limited components in cosmetics had been studied a little, so should be strengthened more.

0. 05). Conclusion :Cordyceps sinensis can inhibit the hepatic fibrosis,and at the same time,doesn't improve the hepatic glycogen synthesis.

Objective:To study the effects of combination of arsenic trioxide and octreotide on the proliferation and apoptosis of hepatocellular carcinoma cells(HCC),and to explore the mechanism of their action on liver cancer.Methods:Heptocellular carcinoma cell SMMC-7721 was treated with arsenic trioxide and octreotide.Cell survival rate was determined by MTT assay;cell cycle and the apoptosis index were determined by flow cytometry (FCM);the morphological changes were observed by transmission electron microscope.Results:The combination of arsenic trioxide and octreotide significantly inhibited the growth of SMMC-7721 and the concentration of each drug in combination is 2.022?mol/L and 8.088?mol/L that was less than single use of arsenic trioxide (3.707?mol/L)and octreotide (22.637?mol/L).The combination of two drugs can arrest HCC in S phases and induce apoptosis.Conclusion:Arsenic trioxide can enhance the antiproliferative effect of octreotide to human HCC and induce apoptosis.

Objective:To study the changes of cell cycle phase and cell cycle phase regulation protein during arsenic trioxide induced apoptosis in hepatoma cells,and to explore mechanisms of arsenic trioxide inhibited HCC.Methods:Cultured in vitre,HCC SMMC-7721 were treated 72h with 2?mol/L arsenic trioxide.The cell cycle and apoptosis index were detected by flow cytometry(FCM);the morphological changes were observed by transmission electron microscope.The immunohistochemistry was used to detect the protein expression of p21、cyclin D_1,cyclinA.Results:SMMC-7721 cell cycle was arrested in G_1 and S phase by arsenic trioxide.Compared with control in the course of apoptosis induced by arsenic trioxide p21 protein expressions rose significantly(control:1.128;arsenic trioxide:3.794),while cyclinD_1,cyclinA protein expressions decreased significantly(control 1:3.647,2.833;arsenic trioxide:1.133,1.179).Conclusion:Arsenic trioxide could disturb cell cycle progression of SMMC-7721 and induce apoptosis.The mechanism may relate to that arsenic trioxide up-regulate expression of p21 and down-regulate expression of cyclinD_1,cyclinA.

Objective To investigate the chnical effect of levonorgestrel-releasing intrauterine system(LNG-IUS) in patients with adenomyosis.Methods A total of eighty patients with adenomyosis were enrolled and underwent LNG-IUS insertion.The degree of dysmenorrhea,amount of menorrhea,size of the uterus,endometrial thickness,hemoglobin as well as hormonal changes and adverse reaction before treatment and after treatment for 3,6,12 months were compared.Results After treatment for3,6,12months,the amount of menorrhea was reduced [(52.4 ± 8.4)％,(22.6 ± 9.8)％,(14.8 ± 8.6)％ vs.(100.0 ±0.0)％],the scores of dysmenorrhea was reduced [(1.2 ± 0.5),(0.8 ± 0.4),(0.3 ± 0.2) scores vs.(2.4 ±0.6) scores],the level of hemoglobin was increased [(104.4 ± 11.4),(109.2 ± 12.6),(118.8 ± 13.4) g/L vs.(88.6 ± 16.5) g/L],the endometrial thickness was thinner [(6.3 ± 1.4),(3.6 ± 0.8),(1.8 ± 0.5) mm vs.(9.2 ± 2.8) mm],the size of the uterus was reduced [(156.8 ± 56.8),(142.4 ± 48.9),(126.6 ± 36.5)cm3 vs.(172.6 ± 62.5) cm3],there was significant difference compared with that before treatment (P ＜ 0.05).After treatment for 3,6,12 months,the levels of folhcle-stimulating hormone,luteinizing hormone and estradiol had no significant difference compared with those before treatment (P ＞ 0.05).After treatment for 3,6,12 months,the level of cancer antigen 125 was significantly reduced compared with that before treatment [(34.6 ± 11.6),(16.6 ± 9.8),(11.7 ± 6.4) kU/L vs.(62.4 ± 24.3) kU/L].Conclusiom The LNG-IUS has exact therapeutic effect in treatment of adenomyosis,and has little influence on ovarian function.It is worth of using in clinic.

Objective To explore the clinical efficacy of ethinylestradiol and cyproterone acetate (Diane-35) combined with mefformin in patients with polycystic ovary syndrome (PCOS).Methods One hundred and sixty patients with PCOS were divided into control group and observation group by random digits table method with 80 cases each.Patients in the control group were given Diane-35 only,while patients in the observation group were given Diane-35 combined with metformin.The triglycerides (TG),total cholesterol (TC),low density lipoprotein cholesterol (LDL-C),fasting insulin (FINS),fasting blood glucose (FBG),follicle-stimulating hormone (FSH),luteinizing hormone (LH) and testosterone (T) of patients in the two groups were measured before treatment and after treatment 6 months,the rates of pregnancy and abortion were recorded.Results TC,LDL-C,FINS,FBG,FSH,LHt and T of both the 2 groups after treatment decreased obviously than before treatment,and TG in observation group decreased obviously than before treatment,there were statistical differences (P ＜ 0.05).There was no statistical difference in TG in control group between before treatment and after treatment (P ＞ 0.05).After treatment,TG,FINS,FBG,LH in observation group were lower than those in control group [(1.0 ± 0.2) mmol/L vs.(1.6 ± 0.8) mmol/L,(15.2 ± 6.3) mU/L vs.(18.9 ± 6.2) mU/L,(4.5 ± 1.1) mmol/L vs.(5.1 ± 0.9) mmol/L,(6.3 ± 3.0) U/L vs.(7.4 ± 2.9) U/L],there were statistical differences (P ＜ 0.05).After treatment,the rate of pregnancy in observation group was higher than that in control group [61.2％(49/80) vs.42.5％(34/80)],there was statistical difference (x2 =5.633,P＜ 0.05).The rote of abortion in observation group was lower than that in control group [6.1％(3/49) vs.17.6％(6/34)],but there was no statistical difference (x2 =1.694,P ＞ 0.05).Conclusion The clinical effect of Diane-35 combined with metformin in patients with PCOS is better than Diane-35 only.

Objective To investigate the clinical characteristics and prognosis of young patients with endometrial cancer.Methods Thirty cases with endometrial cancer were selected as our observed group (age less than 40 years old).Forty cases with endometrial cancer were selected as control group and their age were over 40 years old.The information about clinical characteristics,diagnosis,treatment and prognosis of patients in two groups were collected.Results The rates of primary infertility and less than 3 times of pregnancy in observed group were 30.0％ (9/30),56.7％ (17/30) respectively,higher than that in control group (5.0％(2/40) or 30.0％ (12/40)).The differences were statistically significant (x2 =6.183,5.372 respectively,P ＜ 0.05).The positive rate of estrogen receptor(ER) and progesterone receptor(PR) expression were 93.3％ (28/30) and 86.7％ (26/30) in observed group,higher than that in control(42.5％ (17/40),52.5％ (21/40)respectively).The differences were statistically significant (x2 =5.951,4.721 respectively,P ＜ 0.05).Two groups of patients were statistically significant differences in pathological stage,the pathological type of observed group was mainly stage Ⅰ (76.7％ (23/30)),higher than that of control group (47.5 (19/40),and the difference was statistically significant (x2 =5.464,P ＜ 0.05).There was statistically significant difference between the observed and control group on Cervical infiltration and lymph node metastasis(x2 =5.464,1.018respectively,P ＜ 0.05).Conclusion The infeaility and less than 3 times of pregnancy may be the risk factors of younger endometrial cancer and the pathological type of observed group was mainly stage Ⅰ.However the prognosis is similar between the young and the old patients.

Actins ; metabolism ; Adult ; Breast Neoplasms ; metabolism ; pathology ; surgery ; Diagnosis, Differential ; Female ; Humans ; Immunohistochemistry ; Middle Aged ; Neoplasm Recurrence, Local ; Phyllodes Tumor ; metabolism ; pathology ; surgery ; Vimentin ; metabolism

Adult ; Humans ; Liver Transplantation ; United States

Objective To investigate the correlated factors of prognosis for acute myeloid leukemia (AML).Methods A retrospective analysis of initial 182 patients with AML was performed to assess the relationship between prognostic factors including sex,age,white cell count at diagnosis,immunophenotype,cytogenetics,allo-HSCT and complete remission (CR) after one-course of induction and overall survival (OS) or event free survival (EFS).Results The median age of 182 patients was 49 (14-80) years old,and the median follow up was 9.7 (0.5-75.5) months.There were 107 patients achieved CR after one-course of induction (total response rate was 65.9 ％,120/182),age≥60 years.CD64 negative,unfavorable cyto-genetics and failing to achieve CR after one-course of induction were associated with lower total CR rate.Univariate analysis results demonstrated that age ≥60 years,unfavorable cytogenetics,CD11b expression and failing to achieve CR after one-course of induction had poor influence on 3-years OS and EFS,while the 3-years OS and EFS for patients with CD19 expression,CD64 expression and allo-HSCT were better than that of the patients with negative CD19 and CD64.Multivariate analysis suggested that age,cytogenetics,CD11b,CD64,allo-HSCT and CR after one-course of induction were independent prognostic factors for OS,while cytogenetics,CD64,allo-HSCT and CR after one-course of induction were important on EFS.Conclusions Prognostic analysis depending on AML corelation factors is benifit to individual therapy and can be applied to improve the survival.