50 tracheotomy patients with mechanical breathing were performed respiratory tract control for suitable breathing. Result 5 patients recovered and had spontaneously breathing without respirator in 3~5days, then the trachea cannulas were pulled out in 1~2 weeks. Conclusion effective respiratory tract control may enhance the curative rate and improve patients' life quality.

Objective To study the changes in number and function of peripheral blood T helper cell 17(Th17) in acute urticaria patients .Methods The percentages of Th17 in peripheral blood from 50 acute urticaria patients and 50 healthy persons were ana‐lyzed by immunofluorescence staining and bicolor flow cytometry (FCM ) in vitro .Retinoic acid‐related orphan nuclear receptor γt (RORγt) mRNA from the same research objects were detected by quantitative realtime PCR (qPCR) method .Peripheral blood transforming growth factor‐β(TGF‐β) ,interleukin(IL)‐6 ,IL‐17A and IL‐17F were tested by enzyme linked immunosorbent assay (ELISA) from the same research objects .Results The quantity of percentages of Th17(t= 36 .634 1 ,P< 0 .05) and levels of RORγt mRNA(t=23 .840 1 ,P<0 .05) in urticaria patients were higher than those in healthy persons obviously .Meanwhile ,the levels of TGF‐β(t=15 .521 1 ,P<0 .05) ,IL‐6(t=7 .247 3 ,P<0 .05) ,IL‐17A(t=15 .415 3 ,P<0 .05) and IL‐17F(t=13 .032 1 , P<0 .05) in urticaria patients were higher than those in healthy persons significantly .Conclusion Dysfunction of Th17 in periph‐eral blood may involve in the immunopathogenesis of acute urticaria .

Sox gene family is composed of a class of SRY gene,encoding a series of transcription factors.In the ontogeny,sox genes involved in a variety of development,such as sex determination and differentiation,neural development,cartilage formation.In recent years,researchers found that the abnormal expression of sox gene was related with the development of breast cancer,such as,the overexpression of Sox2 and Sox4 was related with breast cancer; Sox7 and Sox17 in breast cancer could act as tumor suppressor gene,the downregulation of which could activate Wnt/β-catenin signaling pathway.

OBJECTIVE: To analyze the characteristics of adverse drug reaction (ADR) induced by Levofloxacin (LVFX) injection and to promote rational use of drugs in the clinic. METHODS: 137 LVFX injection-induced ADR cases reported in our hospital during 2006～2009 were collected and analyzed statistically with Excel in respect of age, gender, medication and clinical manifestation, etc. RESULTS: ADR cases occurred nearly in all ages of people, and 32.85% were appeared in people over 60 years. Main clinical manifestations were lesion of skin and its appedants (62.04%) and gastrointestinal injury (16.06%). Most of them were mild and medium ADR. CONCLUSION: Great importance should be attached to the rational use of LVFX injection and ADR monitoring to guarantee the safety of patients.

ApolipoptoteinA1isthemaincomponentofhigh-densitylipoproteincholesterolintheplas-ma,which plays an important role in the lipid transportation and metabolism.It is closely related with cardio-vascular disease,liver disease,hereditary amyloidosis and so on.CA1 25 ,as an important biomarker,has been widely used for the diagnosis and monitoring treatment of ovarian cancer.Improved sensitivity and specificity of diagnosis are obtained when it is combined with other tumor markers,especially CA1 25 .As reported recently, it also is related with the generation,progress and prognosis of ovarian cancer.It has been promising to be a new kind of treatment.

Therapeutic effect of short-term administration of IFN-a on non-small cell lung cancer ( NSCLC) was observed . Seventy-two cases of NSCLC patients were randomized into two equal groups. The control and the IFN-a group received the same chemotherapeutic treatments while the latter group were also intramuscularly injected with IFN-? 1 ? 10~(6)U/day for 20 days. Treatment effect rate, the number of white blood cells, the growth of carcinoma cells and the in-terclude time of chemotherapy were studied. Statistically significant difference with each index was observed between the two groups. The results indicated that the short-term administration of IFN-? during chemotherapeutic suppression inhibited tumor growth and made a shortened chemotherapeutic treatment interval acceptable. All showed that administration of IFN-? was feasible and effective in NSCLC chemotherapeutic treatment.

Leukemia is a malignant hyperplasia disease of hematopoietic system. It is characterized that a certain blood cell system proliferates, gets into blood streaming, infiltrates each organism and apparatus and arouse a series of clinical manifestation. In recent years the study of leukemia has discovered that it was correlated with apoptosis of the cells. The roles of XIAP and survivin in apoptosis inhibition have been especially paid attention. It is discovered that they play major roles in the genesis and resistance to chemotherapeutical drug of tumors. We summarize the biological characteristics and the expressions of XIAP and survivin in the children with acute leukemia.

In this paper, the authors analyzed the current problems in domestic hospital nursing human re-source such as widespread erosion, nursing staff slack and others. From the characteristics of nursing ethics and be-havior dynamic analysis, suggest to establish a focus on the humanities concern nursing ethics culture of the people-oriented, fundamentally effectively motivate nurses, specific include: respecting nurses and ethical personality differences;to establish a trust mechanism for nursing staff;meet the needs of nursing staff learning;to establish a nursing staff self management team, thus improve hospital nursing ethics management work, for the nursing staff to provide a stable and efficient working and living environment.

Objective To observe the effect of Chinese herbal compound Yitangkang on the expressions of advanced glyclation end-products (AGEs) and the receptor of advanced glyclation end-products mRNA(RAGE-mRNA) in rats with diabetic nephropathy(DN),and to discuss the mechanism of the formulas in the treatment of DN. Methods The rat model of DN were established by intraperitoneal injection of strepotozotocin(STZ). After 8 weeks intervention with medications, the expression of renal RAGEs mRNA was detected by Real-time PCR, renal RAGE by immunohistochemistry, and renal content of AGEs by spectrophotometry. Results Compared with normal control group, the expression of renal RAGEs mRNA and RAGEs in diabetic nephropathy rats were increased significantly(P<0.01). Compared with DN group, the expression of renal RAGEs mRNA and RAGEs in three doses Yitangkang groups and western medicine group were decreased significantly(P<0.01). Conclusion Chinese herbal compound Yitangkang could protect kidney of diabetic nephropathy rats,and the mechanism is related to its loweirng renal expression of RAGEs mRNA, RAGEs and AGEs content.

ObjectiveTo testify the therapeutic efficacy of acupuncture in treating diarrhea-predominant irritable bowel syndrome (D-IBS) based on brain-gutinteraction.MethodEighty D-IBS patients were randomized into brain-gut interacted acupuncture group (treatment group) and a group with acupoints selected based on conventional syndrome differentiation (control group), 40 in each group. The treatment group was intervened by acupuncture by selecting the thirteen ghost acupoints (invented by SUN Si-miao) plus Tianshu (ST25), Dachangshu (BL25), etc., while the control group was by acupuncture based on conventional point selection, including Zusanli (ST36),Tianshu (ST25), Dachangshu (BL25), Ganshu (BL18), Pishu (BL20), etc., three times a week, and 10 sessions as a treatment course. The clinical efficacies were evaluated after 2 treatment courses.ResultThe two groups were generally equal to each other incomparing each dimension of the Short-form Health Survey (SF-36) before treatment, while the scores were increased after treatment in both groups; the treatment group was significantly superior to the controlgroup in comparing the scores ofrole-physical,bodily pain, general health, vitality, social role functioning, and reported health transition from the SF-36 (P<0.05).ConclusionAcupuncture based on brain-gut interaction can produce a more significant effect in treating D-IBS compared to conventional acupuncture.