The aim of this study is to investigate the apoptotic inhibition and its molecular mechanism of dexamethasone (DEX) acting on cisplatin (CDDP)-induced apoptosis of human lung adenocarcinoma cell SPC-A1; SPC-A1 cells were pre-cultured in vitro for 24 hours with DEX in different concentrations and then CDDP was added in different concentrations for culturing for further 48 hours. The survival rates of the cells were determined by MTT. The expression of serum/glucocorticoid-induced kinase (SGK-1) and mitogen-activated protein kinase phosphatase-1 (MKP-1) in SPC-A1 cells after being cultured by 1 micromol/l DEX at different time was detected by semi-quantitative RT-PCR technology. The expression of glucocorticoid receptor (GR) in SPC-A1 cells was measured by immunohistochemistry (IHC) with biotin-labeled anti-GR. The results of MTT showed that SPC-A1 cells had resistance to CDDP-induced apoptosis with pre-cultured DEX and the resistance intensity presented DEX concentration-dependent. The expressing quantity of SGK-1 in SPC-A1 cells stimulated by DEX could be elevated and increased with intention of time, but the express of MKP-1 was not detected. Up-regulated expression of GR in SPC-A1 cells stimulated by DEX was detected by IHC. The number of cells expressing GR in SPC-A1 cells was significantly higher than that in the control group. The results showed that DEX inhibited apoptosis of SPC-A1 cells induced by CDDP. The possible molecular mechanism is that elevated expression of GR induced by DEX up-regulates the expression of SGK-1 which locates at the downstream of anti-apoptosis pathway. The apoptosis resistance of SPC-A1 cells may account for all above the factors.
Adenocarcinoma ; pathology ; Apoptosis ; drug effects ; Cell Line, Tumor ; Cisplatin ; pharmacology ; Dexamethasone ; pharmacology ; Dual Specificity Phosphatase 1 ; metabolism ; Humans ; Immediate-Early Proteins ; metabolism ; Lung Neoplasms ; pathology ; Protein-Serine-Threonine Kinases ; metabolism ; Receptors, Glucocorticoid ; metabolism ; Up-Regulation

Objective To summarize experience of periopertively nursing children with floating thumb treated with composite tissue flap transplantation. Method Health education was done to 5 children with floating thumb treated with composite tissue flap grafting as well as their families including preparing preoperational checks, preventing crisis of blood circulation, medication and pain nursing and paying attention to rehabilitation instruction. Result All thumbs survived successfully and one patient developed with crisis of blood circulation, which disappeared in 15 minutes. Conclusion Good preoperative nursing and effective health instruction are critical for nursing of the children with floating thumb treated with composite tissue flap transplantation.

BACKGROUND: Na+/dicarboxylate transporter protein (NaDC3) participates in the metabolism of energy, which may has effect on cellular senescence; however, the mechanism is not clear. OBJECTIVE: To investigate the role of human NaDC3 in cellular senescence by means of cell cycle and P16 measurements. DESIGN, TIME AND SETTING: The single sample observation was performed in the Nanjing Drum Tower Hospital from December 2007 to May 2008. MATERIALS: The WI-38 cell was purchased from ATCC Company, the retrovirus vector pLNCX2, sense vector PLNCX2-hNaDC3 and antisense vector PLNCX2-AshNaDC3 was provided by Clontech Company. METHODS: hNaDC3 was introduced into WI-38 cells with retroviral virus-mediated transfection. The integration and expression of exotic gene were confirmed. Whereafter, to examine the effects of hNaDC3 on cellular senescence of WI-38 cells by cell cycle and expression of P16. MAIN OUTCOME MEASURES: The integration and expression of hNaDC3, the cell morphology and generation of WI-38, the cell cycle and expression of P16 were observed. RESULTS: The results of Northern blot and Western blot revealed that hNaDC3 were expressed in WI-38/ hNaDC3 cells at gene and protein level, while, in WI-38/As hNaDC3 cells, there were integration of hNaDC3 mRNA with not protein expression. Compared with the control cells, WI-38 cells transfect with hNaDC3 cDNA showed cell generation decreased by eight to twelve passages, which in a low cell growth rate with cell cycle arrested at G1 phase, and the expression of P16 was elevated. CONCLUSION: Human NaDC3 gene may contribute to the process of cellular senescence of human diploid fibroblasts by increasing expression of P16.

Objective To study the relationship between cognitive dysfunction and depression and event-related potential(ERP) in patients with silent brain infarction(SBI).Methods 68 SBI patients were tested by the Hamilton Depression Rating Scale for Depression(HAMD),scale of mini mental state examination(MMSE)and ERP;and the results were compared with those in 55 patients without cerebral infarction(control group).Results In the SBI group,the HAMD score was ≥17 in 31(45.6%)cases(depression subgroup)and

The necessity of the Electrocardiograph anti-interference performance test are introduced,and the principle and method of anti-interference performance test based on standard YY 1139-2000 are also provided.Through the experiment of anti-interference,the reliability and practicality of our self-made testing device is confirmed.

Objective:To investigate the interpersonal attribution style of the baccalaureate nursing female students(BNFS)and to explore the difference of attribution style between the female students majoring in clinic medicine and nursing.Methods:A cross-sectional study was carried out by using the Interpersonal Relationship Subscale of Multidimensional-Multiattributional Causality Scale by Lefcourt et al. 500 college female students,from clinic medicine and nursing science of Central South University Xiangya School of Medicine, were recruited by using stratified random sampling.Results:The higher Grade, the higher Interpersonal Relationship Attribution Score (IRAS) of BNFS(44.7?7.8~50.8?9.5,45.9?8.0~51.2?7.9). There was no statistical difference in IRAS of the female students majoring in clinic medicine and nursing science (P=0.15～0.81), but the different Grades had statistical difference in IRAS(P

asthma is a kind of chronic mucosal inflammation and characterized by eosinophilia,hypertrophy of goblet cell,increment of mucous secretion,reversible airway obstruction and hyperresponsiveness to external and endogenous stimuli.Of them,the recruitment and activation of eosinophil have an important role in the pathogenesis of asthma;the aim of this paper is to review the advancement of recruitment of eosinophil in the pathogenesis of asthma,and make a new method for the treatment of asthma.

Objective To find the best methods to make the extraction of alternaria alternate. Methods Alternaria alternate was harvested from air and identified, cultured at 26 ℃ for four weeks. Protein was extracted by different methods and its concentration was determined using the Bradford assay, its bioactivity was tested by RAST inhibition, and residual proteins were analyzed by SDS-PAGE.Results The extraction methods like grinder(a), grinder plus ultrasonic(b), ground with liquid nitrogen(c) and liquid nitrogen plus ultrasonic(d) were applied in the extraction procedure. The concentrations of protein extractions were as follows (g/mL): a was 0.44?0.04;b was 0.75?0.03;c was 0.72?0.03 and d; 1.29?0.05. From SDS-PAGE, we found that the most component in the extraction was that obtained by liquid nitrogen plus ultrasonic. From RAST, 50% inhibition was as follows(g/mL): a was 8.5, b was 9.4, c was 7.0,d was 3.7. Conclusion Ground with liquid nitrogen plus disintegration by ultrasonic was the most effective method of extraction.

Objective:To search for a method for analyzing medical service quality of different disease entities using the first page data of medical records.Methods: Based on documents review and real data analysis,the basic principles,methods and steps of disease entity dividing and selecting,evaluation index selecting,and calculating were introduced.Special attention was paid to certain problems in the discussion.Results: When the inpatients of selected disease entities covered 60% of total hospitalized patients,the medical quality information included exceeded 80% of the total information.For 97.66% disease entities,their inpatient cost and length of hospital stay were logarithmic-normally distributed.Conclusion: Dividing disease entities by fixed digits of ICD-9 code is easily performable.This method should be used when the disease entities include about 60% of total inpatients,and geometric mean should be used in calculating average length of hospital stay and average cost.

Purpose:To study analgesia effect ,adverse reactions and life quality changes of transdermal fentanyl in patients with cancer pain.Methods:30 patients with moderate or severe cancer pain were treated by transdermal fentanyl .The pain intensity, quality of life and adverse reactions were observed before and after the treatment.Results:After using transdermal fentanyl ,all the patients obtained moderate to good remission.The excellent,marked and moderate rates were 43.33%,53.33% and 3.33% respectively. The adverse reactions included nausea, vomiting,constipation,dizziness and lethargy.The rate of the adverse reactions was less.The quality of life improved markedly. Conclusions:Transdermal fentanyl is a safe,effective and simple way to relieve cancer pain in patients with moderate or severe pain.It can improve the quality of life markedly.Its adverse reactions are mild.