Objective To investigate the effects of mindfulness behavior training on mood states and quality of life in old patients post fixation for hip fracture. Methods Patients were randomly divided into experiment group (n=30) and control group (n=30). The control group received routine rehabilitation, while the experiment group received mindfulness practice therapy in addition. They were evaluated with Profile of Mood States (POMS), Short Form of Health Survey (SF-36), and Mindful Attention Awareness Scale (MAAS) before and 5 weeks after treatment. Results There was no difference in the scores of POMS, MAAS and SF-36 before treatment (P>0.05). The scores of MAAS and SF-36, the subscores of tension-anxiety, depression-dejection, fatigue-inertia and vigor-activity of POMS improved more in the experiment group than in the control group (P<0.05). Conclusion Mindfulness behavior training can improve the mood state and quality of life of old patients post fixation for hip fracture.

Objective　To evaluate the diagnostic value of 99Tcm-HL91 in experimental tumor and inflammation models comparing with 99Tcm-MIBI. Methods　Three kinds of solid neoplasm bearing mice (Ehrlich carcinoma bearing mice, H22 carcinoma bearing mice and human ovarian COC1 neoplasm bearing nude mice) and two inflammation models (chemical and bacterial inflammation) underwent static whole body planar imaging at 1 and 4 hours after injection of 99Tcm-HL91. Two kinds of neoplasm bearing mice (Ehrlich carcinoma bearing mice,H22 carcinoma bearing mice) and two inflammation models (chemical and bacterial inflammation) underwent static planar imaging after injection of 99Tcm-MIBI, at early phase (10～20 minutes) and delayed phase (2 h). All of the mice were sacrificed at 4 h. The tumors, or inflammatory lesions, blood and contralateral normal muscles served as controls were removed, weighted and the radioactivity was measured. ROIs were drawn around tumor, inflammatory lesions and contralateral muscles in planar images, and the radioactivity ratios of target (tumor or inflammatory lesions)-to-blood (T/B), target-to non target (contralateral muscles) i.e. T/NT were calculated. Results　Neoplasms were clearly visible in planar images at 1 and 4 h after injection of 99Tcm-HL91 in all tumor models. At the same time inflammatory lesions could not be seen clearly. Neoplasms were seen in delayed phase in 99Tcm-MIBI group, but it was not easy to distinguish them from inflammation. The T/B ratios and T/NT ratios of 99Tcm-HL91 tumor model groups were significantly higher than that of 99Tcm-MIBI tumor model groups. The T/NT ratios of tumors were significantly higher than that of inflammatory lesions in 99Tcm-HL91 groups. Conclusion　Compared with 99Tcm-MIBI imaging, 99Tcm-HL91 is of much more diagnostic value in detection of certain solid neoplasms, and can distinguish neoplasm from inflammation.

Objective To investigate the emotional intelligence training training's impact on medical students' ethics education.Methods Givingnthe undergraduate and graduate students of Grade 2009,2010 and 2011 emotional intelligent training and distributing questionnaires on ethics education and comparing the result with that of 2006,2007,2008 undergraduate and graduates.Covering issues of medical ethics and the understanding of students' role in alleviating the doctor-patient relationship in medical ethics,evaluation of medical ethics in today's society,the doctor of professional ethics,medical ethics education approaches and problems and so on.By comparing the results of the questionnaire students' knowledge of medical ethics are evaluated and their emotional intelligence training in ethics education role are analyzed.Research data for entry to a database of Excel 2013,using SPSS 19.0 statistical software for analysis,count data taken x2 test,P＜0.05 was considered statistically significant.According to the questionnaire topic A representative proportion of medical students is selected to measure the level of ethics and the role of ethics education in medical students training in emotional intelligence.Results the students of 2006,2007 and 2008 who selected A of five topics were 54.78％(1 278/2 333),79.47％(1 854/2 333),68.41％(1 596/2 333),86.28％(2 013/ 2 333),13.12％(306/2 333); while the students of 2009,2010 and 2011 who selected A of five topics were 73.70％(1 777/2 411),88.68％(2 138/2 411),81.54％(1 966/2 411),93.78％(2 261/2 411),71.75％(1 730/2 411).The difference was statistically significant,indicating that 2009,2010 and 2011 medical students' cognitive level of ethicshas been nore significantly improved than the students of Grade 2006,2007 and 2008.Conclusion EQ training can significantly improve the effectiveness of medical ethics education for medical students and it is worth promoting.

Objective To analyze the clinical features of Takayasu′s arteritis (TA) and to evaluate the usefulness of 2D color Doppler flow imaging (2D CDFI) and digital subtraction angiography (DSA) in diagnosing TA.Methods Fifty eight patients with TA were analyzed.In 26 cases,the 2D CDFI findings were compared with that of DSA.Results ① One hundred and eighty two peripheral arteries were involved in 58 patients including 176 arteries with stenosis or occlusion,mainly with aortic arch syndrome and left side involved.② Compared with DSA,2D CDFI had a higher sensitivity (88%) in diagnosing stenosis and occlusion of vessel cavity in aorta and its main branches,and had a positive predictive value of 100% and anegative predictive value of 0% respectively.Conclusion 2D CDFI has some outstaning features as non invading,safety,cheapness and so on in diagnosing TA,compared with DSA that is usually used as "the gold standard" for the definitive diagnosis of this disease.2D CDFI is able to depict vessel wall thickening,which is a major pathologic feature of the disease and it is suitable for long term repeated follow up.

DEAD box family,characterized by the conserved motif D (Asp)-E (Glu)-A (Ala)-D (Asp),are putative RNA helicases,which participate in all cellular processes regulated by RNAs.Studies show that there are examples of dysregulation of DDX family expression in various types of cancer.They are involved in the occurrence and evolution of tumor,and take part in tumor cell proliferation,apoptosis,cycle,invasion,metastasis and metabolism,which can be acted as new molecular markers for the diagnosis of tumor,the evaluation of malignancy and the prediction of clinical prognosis and also provide new potential molecular targets for tumor therapy.Here,we reviewed the structure,biochemical function and the relationship with tumor of DDX family.

Objective To evaluate the clinical effect of cytokine induced killer cellular immunotherapies ( CIK) on the patients with advanced lung cancer.Methods Peripheral blood mononuclear cell from 32 cases of patients with advanced lung cancer were isolated.Cells were cultured in DMEM supplemented with IFN-γ,IL-2,anti-CD3 monoclonal antibody and other cytokines,and transfused back into the patients in several times after 15 days of culture.Subsequently,we observed the size of tumor,the change of immunologic function by CIK treat-ment,the status of clinical symptoms,and the toxic and side effect.The clinical curative effect was also evaluated. Results The symptoms in 32 patients who received CIK therapy were reduced,and the side effects were light. Immunophenotype analysis showed that CD3 /CD4 coexpression were found in 34.4%patients,CD3 /CD8 co-expression in 68.2%,and CD3 /CD56 co-expression in 13.7% patients.CT images about 15 days after CIK cell transfusion showed that pleural effusion were decreased.Clinical evaluation effective rate was 56.0%,and disease control rate( CR +PR +SD) was 84.3%.Conclusion CIK cell immunotherapies of cancer patients can improve immunologic function of cancer patients and inhibit tumor growth and relieve the clinical symptom of patients,and improve the quality of life.

The etiology of pulmonary fibrosis is complex. The occurance and development of this disease involve many cell signal transduction pathways, including MAPK signaling pathway, JAK-STAT signaling pathway, Smad signaling pathway, PI3K-Akt signaling pathway, NF-κB signaling pathway and so on. Numbers of experiments have shown that intervention in signal transduction pathways with TCM can delay the progression of fibrosis, which provides good therapeutic methods for this disease. This article reviewed study on TCM intervention in different signal transduction pathways in the pulmonary fibrosis in China.

Objective To investigate the effect of ethyl pyruvate (EP) on the expression of caspase-3 in the hippocampus and the learn-ing and memory ability in neonatal rats with hypoxic ischemic brain damage (HIBD). Methods 90 Wistar rats of 7 days old were randomly divided into sham operation group (n=15), HIBD group (n=15), EP1 group (n=15, 10 ml/kg), EP2 group (n=15, 30 mg/kg), EP3 group (n=15, 50 mg/kg) and EP4 group (n=15, 100 mg/kg). The model was established with Rice's method. 30 minutes before operation, and every 24 hours after operation, EP groups were injected with 10 ml/kg, 30 mg/kg, 50 mg/kg, 100 mg/kg EP in abdomen respectively, for 2 weeks. Af-ter treatment, the caspase-3 positive cells were observed by immunohistochemical staining, and the latency and the times crossing the target quadrant were tested by Morris water maze test. Results The caspase-3 positive cells were less in EP groups than in HIBD group (P<0.05), expect EP1 group (P>0.05), especially in EP3 group (P<0.01). The latency and the times crossing the target quadrant were better in EP groups than in HIBD group (P<0.05), expect EP1 group (P>0.05), especially in EP3 group (P<0.01). Conclusion Ethyl pyruvate can de-crease the expression of caspase-3 in hippocampus, and improve the ability of memory and learning in neonatal rats with HIBD.

Objective To investigate the influence of salvianolic acid B on the expressions of TGF-β1/Smad signaling pathway related proteins in human lung fibroblasts,and to explore the mechanism of the inhibitory effect of Sal B on TGF-β1-induced lung fibroblast activation.Methods The human embryonic lung fibroblasts (MRC-5) were cultured in vitro and randomly divided into control group ( the cells were cultured with DMEM without TGF-β1 or Sal B),Sal B group (the cells were cultured with 10μmol·L-1 Sal B),TGF-β1 group (the cells were cultured with 10μg·L-1 TGF-β1),and TGF-β1 (10μg·L-1 )+ Sal B (10μmol·L-1 )group.The protein levels of p-Smad2,p-Smad3,TβRⅠ,and Smad7 in the fibroblasts in various groups were detected by Western blotting method.Results Compared with control group,the expression levels of p-Smad2,p-Smad3,and TβRⅠproteins in TGF-β1 group were significantly increased (P<0.05),and the expression level of Smad7 protein was decreased (P<0.05).Compared with control group,the expression levels of p-Smad2,p-Smad3,TβRⅠ,and Smad7 proteins in lung fibroblasts in Sal B group had no significant change (P>0.05).Compared with TGF-β1 group,the expression levels of p-Smad2,p-Smad3,and TβRⅠ in TGF-β1+ Sal B group were descended (P<0.05),and the expression level of Smad7 was increased (P<0.05).Conclusion Sal B could suppress the TGF-β/Smad signaling pathway in lung fibroblasts and to inhibit the TGF-β1-induced lung fibroblast activation.

OBJECTIVE:To provide reference for rational use of Fentanyl transdermal patch in the clinic. METHODS:By ex-tracting data from hospital information system and digital medical record management system,medical records of 150 cancer pain inpatients receiving Fentanyl transdermal patch were collected from our hospital during Jun.-Dec. 2014,and then investigated and analyzed retrospectively. RESULTS:Numeric rating scale (NRS) score of 150 cancer pain patients receiving Fentanyl transdermal patch were as follows:pain scores of 17 patients (11.3%) were 1-3 points,those of 63 patients (42.0%) were 4-6 points and those of 5 patients(3.3%)were 7-10 points;the rest of the patients were not rated. Among those 150 cases,88 cases were well tol-erated with opioid and 62 patients were considered to be opioid intolerant. Those intolerant patients were who could not be treated orally or could not bore side effects of opioid hepatic or renal dysfunction. 25 cases used Fentanyl transdermal patch without defi-nite evidence. As to drug combination,22 cases additionally received Morphine hydrochloride injection;12 cases additionally re-ceived Bucinnazine hydrochloride injection;8 cases additionally received Morphine tablet;10 cases additionally received Oxycodo-ne hydrochloride sustained-release tablets and Morphine hydrochloride sustained-release tablets;8 cases additionally received Oxyco-done and acetaminophen tablets;3 cases additionally received Carbamazepine tablets. CONCLUSIONS:Fentanyl transdermal patch are widely used for cancer pain treatment in our hospital. There still is irrational use of it,such as absence of pain score before use,no medication indication,irrational drug combination. Physicians should obey medication indication strictly,and clinical phar-macists should strengthen communication with physicians and pay attention to medication education for patients so as to guarantee rational drug use.