Amphibian is playing a very important role in the field of environmental toxicological studies. In the present paper, taking the Xenopus laevis, a model animal of amphibian, as an example, reviewed the application of amphibian in environmental toxicology, such as acute toxicity, frog embryo teraogenesis array-Xenopus(FETAX), micronucleus test, environmental endocrine disruptors detection.